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Despite anatomical and functional similarities between human and mouse adrenal glands, notable species-specific differences exist. Here, we leveraged spatial transcriptomics (10\u00d7 Genomics Visium) to comprehensively map gene expression in adult human and mouse adrenal glands, aiming to identify canonical marker genes conserved across species. The analysis was based on a 31-year-old female human sample (GEO dataset) and four 10-week-old male CD-1 mice. Human adrenal sections were processed using optimal cutting temperature (OCT) embedding, whereas mouse adrenal sections were processed as formalin-fixed paraffin-embedded (FFPE) samples, highlighting differences in sample preparation. Using unsupervised clustering of spatial gene expression data, we delineated distinct adrenal cortex and medulla zones in both species, confirming known zonation patterns. Our cross-species analysis revealed highly conserved spatial expression of key known marker genes characteristic of the adrenal cortex (e.g.,\n                    <jats:italic>CYP11B2<\/jats:italic>\n                    for ZG,\n                    <jats:italic>CYP11B1<\/jats:italic>\n                    for ZF) and medullary chromaffin cells (e.g.,\n                    <jats:italic>TH<\/jats:italic>\n                    ), as well as a core set of additional marker genes previously less characterized in adrenal biology. By integrating transcriptional profiles, we generated a catalogue of conserved canonical marker genes that define adrenal zonation and function in both humans and mice. These results highlight the fundamental molecular conservation of adrenal gland organization and support the translational value of mouse models in adrenal research. Our findings provide new insights into the evolutionary preservation of adrenal function and a valuable resource for studies on adrenal physiology and disease.\n                  <\/jats:p>","DOI":"10.1007\/s00418-025-02446-6","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T11:34:10Z","timestamp":1766576050000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of conserved canonical marker genes in human and mouse adrenal glands using Visium spatial transcriptomics"],"prefix":"10.1007","volume":"164","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5506-2397","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Blatkiewicz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0150-3665","authenticated-orcid":false,"given":"Marta","family":"Szyszka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4388-286X","authenticated-orcid":false,"given":"Szymon","family":"Hryhorowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7560-9350","authenticated-orcid":false,"given":"Joanna","family":"Suszy\u0144ska-Zajczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3025-4717","authenticated-orcid":false,"given":"Andrea","family":"Porzionato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7243-2473","authenticated-orcid":false,"given":"Adam","family":"Plewi\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludwik K.","family":"Malendowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2525-5777","authenticated-orcid":false,"given":"Marcin","family":"Rucinski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"issue":"8","key":"2446_CR1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1798","volume":"14","author":"B Altieri","year":"2024","unstructured":"Altieri B, Secener AK, Sai S, Fischer C, Sbiera S, Arampatzi P, Kircher S, Herterich S, Landwehr LS, Vitcetz SN, Braeuning C, Fassnacht M, Ronchi CL, Sauer S (2024) Single-nucleus and spatial transcriptome reveal adrenal homeostasis in normal and tumoural adrenal glands. 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