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Following trials to assess speed at the first ventilatory threshold (VT<jats:sub>1<\/jats:sub>) on a 0% gradient and -10% gradient, participants completed two trials: (i) level trial (LVL): 120-min of running at 0% gradient and (ii) downhill trial (DH): 120-min of running with 90-min at 0% gradient and 6\u2009\u00d7\u20095-min intervals at -10% gradient (DH). Running was conducted at 90% of VT<jats:sub>1<\/jats:sub> speed for respective gradients. Speed at VT<jats:sub>1<\/jats:sub> on 0% gradient, perceived leg muscle soreness, and maximal voluntary isometric knee extensor torque were assessed pre- and post-prolonged exercise.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>An effect of time was observed for speed at VT<jats:sub>1<\/jats:sub> (\u2206-6.2\u2009\u00b1\u20093.6% in LVL and \u2206-7.6\u2009\u00b1\u20093.2% in DH, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), with no effect of condition (LVL vs. DH,<jats:italic> P<\/jats:italic>\u2009=\u20090.382), or time \u00d7 condition interaction (<jats:italic>P<\/jats:italic>\u2009=\u20090.295). Reduced metabolic energy expenditure (metabolic power) significantly contributed to the reduced speed at VT<jats:sub>1<\/jats:sub> in both trials (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), whereas changes in running economy (energetic efficiency) did not (<jats:italic>P<\/jats:italic>\u2009=\u20090.228).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Prolonged moderate-intensity running reduced speed at the moderate-to-heavy intensity transition, but this was not exacerbated by increased eccentric load. Reduced speed at the moderate-to-heavy intensity transition following prolonged running was primarily attributed to decreased metabolic power.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s00421-025-05792-4","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T15:01:45Z","timestamp":1745938905000},"page":"2897-2910","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Prolonged running reduces speed at the moderate-to-heavy intensity transition without additional reductions due to increased eccentric load"],"prefix":"10.1007","volume":"125","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8462-8849","authenticated-orcid":false,"given":"Andrew M. 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