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High-density electromyographic signals were recorded and decomposed into MU discharge times. We calculated mean discharge rate (MDR), the coefficient of variation for interspike interval (CoV ISI), and the standard deviation of the filtered cumulative spike train (SD of fCST). In addition, the neural drive within and between muscles was estimated from the cross-correlation of the fCST.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Analysis of variance indicated that MVC was greatest at the long length and torque steadiness was worst at the intermediate length (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Linear mixed models revealed that BF exhibited greater MDR and variability in neural drive (SD of fCST), whereas the MUs in ST displayed greater discharge rate variability (CoV ISI) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Cross-correlation of the estimated neural drives to ST and BF was relatively low, suggesting independent neural control of the two muscles. Moreover, the variability in neural drive for ST was more strongly correlated with torque steadiness (CoV torque) than that for BF.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The findings indicate that MU discharge characteristics differed for ST and BF across knee-joint angles, with each muscle receiving a distinct neural drive highlighting the importance of muscle-specific training strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00421-025-05953-5","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T09:28:29Z","timestamp":1756978109000},"page":"839-852","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Independent neural drives and distinct motor unit discharge characteristics in hamstring muscles during isometric knee flexion"],"prefix":"10.1007","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5974-7948","authenticated-orcid":false,"given":"Chrysostomos","family":"Sahinis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3731-8998","authenticated-orcid":false,"given":"Ioannis G.","family":"Amiridis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7883-2697","authenticated-orcid":false,"given":"Dario","family":"Farina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7881-2397","authenticated-orcid":false,"given":"Roger M.","family":"Enoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8179-6340","authenticated-orcid":false,"given":"Eleftherios","family":"Kellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,9,4]]},"reference":[{"key":"5953_CR1","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1249\/MSS.0000000000003377","volume":"56","author":"IG Amiridis","year":"2024","unstructured":"Amiridis IG, Kannas T, Sahinis C et al (2024) More variability in tibialis anterior function during adduction of the foot than dorsiflexion of the ankle. 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