{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:48:18Z","timestamp":1789130898313,"version":"build-2803163510"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","award":["National Institute for Health and Care Research"],"award-info":[{"award-number":["National Institute for Health and Care Research"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Appl Physiol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Postural changes elicit well-described haemodynamic effects on plasma volume, affecting blood-based hydration variables. While the time required for plasma volume stabilisation is well-established for supine rest, less is known for blood samples collected in a seated position, as is commonplace in physiology and nutrition experiments. Seventeen healthy participants (9 males; 8 females) stood stationary for 20\u00a0min in an upright position before walking on a treadmill at 4\u00a0km\/h for 20\u00a0min (to simulate active travel to blood sampling locations). After walking, participants sat upright (within 7\u2009\u00b1\u20091\u00a0s), and venous blood samples were drawn from a cannula at 0, 5, 10, 20, 30 and 40\u00a0min after sitting. Time points were compared to 40\u00a0min. At 0 and 5\u00a0min, blood (0\u00a0min: -3.3%;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001; 5\u00a0min: -1.2%;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.034) and plasma (0\u00a0min: -5.9%;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001; 5\u00a0min: -1.7%;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.035) volume were lower than 40\u00a0min values. Conversely, plasma osmolality was higher at 0\u00a0min (+\u20090.61%;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.005) and 5\u00a0min (+\u20090.55%;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.007), whilst blood\/plasma volume and plasma osmolality were not different from 10\u00a0min onwards. Plasma sodium, potassium and chloride concentrations were not different from 40\u00a0min throughout the sitting period (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009\u2265\u20090.056). These data demonstrate that blood\/plasma volume, as well as plasma osmolality were affected by posture and\/or walking, but all are not significantly different from 40\u00a0min within 10\u00a0min of seated rest. Therefore, studies involving blood-based hydration measures taken in an upright seated position should allow at least 10\u00a0min of postural control prior to sampling.\n                  <\/jats:p>","DOI":"10.1007\/s00421-025-06048-x","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T11:55:17Z","timestamp":1763726117000},"page":"2155-2160","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["At least 10\u00a0minutes of seated rest is required for stabilisation of blood-based hydration biomarkers"],"prefix":"10.1007","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0285-5762","authenticated-orcid":false,"given":"Kirsty M.","family":"Reynolds","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1458-5244","authenticated-orcid":false,"given":"Samantha N.","family":"Rowland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7473-5999","authenticated-orcid":false,"given":"Mark P.","family":"Funnell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0484-2953","authenticated-orcid":false,"given":"Tom","family":"Clifford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8791-0167","authenticated-orcid":false,"given":"Liam M.","family":"Heaney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3941-9426","authenticated-orcid":false,"given":"Stephen A.","family":"Mears","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0923-6362","authenticated-orcid":false,"given":"Stephen J.","family":"Bailey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6514-5823","authenticated-orcid":false,"given":"Lewis J.","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"6048_CR1","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1002\/cphy.c130017","volume":"4","author":"SN Cheuvront","year":"2014","unstructured":"Cheuvront SN, Kenefick RW (2014) Dehydration: physiology, assessment, and performance effects. 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This report is independent research by the National Institute for Health Research. MPF is funded by the National Institute for Health and Care Research (NIHR) Applied Research Collaboration East Midlands (ARC EM). The views expressed in this publication are those of the authors and not necessarily those of the NIHR, NHS, or the Department of Health. LJJ has current\/previous funding from Entrinsic Beverage Company LLC, Entrinsic Bioscience LLC, Herbalife Europe Ltd, Bridge Farm Nurseries, Decathlon SE, PepsiCo Inc., Volac International; has performed consultancy for PepsiCo Inc. and Lucozade, Ribena Suntory; and has received conference fees from PepsiCo Inc. and Danone Nutricia. In all cases, monies have been paid to LJJs institution and not directly to LJJ. SAM has current\/previous funding from Entrinsic Beverage Company LLP and Herbalife Europe Ltd and has received consultancy fees from Unilever. SNR was supported by external research funding awarded to LMH from Biopolis S.L. The authors declare no other conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}