{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:36:03Z","timestamp":1791520563995,"version":"4.3.4"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2003,5,28]],"date-time":"2003-05-28T00:00:00Z","timestamp":1054080000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2003,5,28]],"date-time":"2003-05-28T00:00:00Z","timestamp":1054080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pflugers Arch - Eur J Physiol"],"published-print":{"date-parts":[[2003,8]]},"DOI":"10.1007\/s00424-003-1097-9","type":"journal-article","created":{"date-parts":[[2003,11,21]],"date-time":"2003-11-21T12:38:55Z","timestamp":1069418335000},"page":"553-558","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":120,"title":["A novel approach to in situ characterization of pancreatic \u03b2-cells"],"prefix":"10.1007","volume":"446","author":[{"given":"Stephan","family":"Speier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Rupnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2003,5,28]]},"reference":[{"key":"1097_CR1","doi-asserted-by":"crossref","unstructured":"Sakmann B, Stuart G (1995) Patch-pipette recordings from the soma, dendrites and axon of neurons in brain slices. In: Sakmann B, Neher E (eds) Single channel recording. Plenum Press, New York, 199\u2013211","DOI":"10.1007\/978-1-4419-1229-9_8"},{"key":"1097_CR2","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1007\/BF00374463","volume":"420","author":"Schneggenburger","year":"1992","unstructured":"Schneggenburger R, Lopez-Barneo J (1992) Patch-clamp analysis of voltage-gated currents in intermediate lobe cells from rat pituitary thin slices. Pflugers Arch 420:302\u2013312","journal-title":"Pflugers Arch"},{"key":"1097_CR3","doi-asserted-by":"crossref","first-page":"2314","DOI":"10.1523\/JNEUROSCI.17-07-02314.1997","volume":"17","author":"Moser","year":"1997","unstructured":"Moser T, Neher E (1997) Rapid exocytosis in single chromaffin cells recorded from mouse adrenal slices. J Neurosci 17:2314\u20132323","journal-title":"J Neurosci"},{"key":"1097_CR4","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1113\/jphysiol.1970.sp009207","volume":"210","author":"PM Dean","year":"1970","unstructured":"Dean PM, Matthews EK (1970) Glucose-induced electrical activity in pancreatic islet cells. J Physiol (Lond) 210:255\u2013264","journal-title":"J Physiol (Lond)"},{"key":"1097_CR5","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0079-6107(89)90013-8","volume":"54","author":"Ashcroft","year":"1989","unstructured":"Ashcroft FM, Rorsman P (1989) Electrophysiology of the pancreatic beta-cell. Prog Biophys Mol Biol 54:87\u2013143","journal-title":"Prog Biophys Mol Biol"},{"key":"1097_CR6","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/978-1-4684-5314-0_14","volume":"211","author":"Trube","year":"1986","unstructured":"Trube G, Rorsman P (1986) Calcium and potassium currents recorded from pancreatic beta-cells under voltage clamp control. Adv Exp Med Biol 211:167\u2013175","journal-title":"Adv Exp Med Biol"},{"key":"1097_CR7","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/BF00378654","volume":"420","author":"Gillis","year":"1992","unstructured":"Gillis KD, Misler S (1992) Single cell assay of exocytosis from pancreatic islet B cells. Pflugers Arch 420:121\u2013123","journal-title":"Pflugers Arch"},{"key":"1097_CR8","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1111\/j.1469-7793.1999.00717.x","volume":"521","author":"S G\u00f6pel","year":"1999","unstructured":"G\u00f6pel S, Kanno T, Barg S, Galvanovskis J, Rorsman P (1999) Voltage-gated and resting membrane currents recorded from B-cells in intact mouse pancreatic islets. J Physiol (Lond) 521:717\u2013728","journal-title":"J Physiol (Lond)"},{"key":"1097_CR9","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/j.1469-7793.2000.00509.x","volume":"528","author":"SO G\u00f6pel","year":"2000","unstructured":"G\u00f6pel SO, Kanno T, Barg S, Weng XG, Gromada J, Rorsman P (2000) Regulation of glucagon release in mouse-cells by KATP channels and inactivation of TTX-sensitive Na+ channels. J Physiol (Lond) 528:509\u2013520","journal-title":"J Physiol (Lond)"},{"key":"1097_CR10","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1111\/j.1469-7793.2000.00497.x","volume":"528","author":"SO G\u00f6pel","year":"2000","unstructured":"G\u00f6pel SO, Kanno T, Barg S, Rorsman P (2000) Patch-clamp characterisation of somatostatin-secreting-cells in intact mouse pancreatic islets. J Physiol (Lond) 528:497\u2013507","journal-title":"J Physiol (Lond)"},{"key":"1097_CR11","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1113\/jphysiol.1993.sp019966","volume":"472","author":"C \u00c4mm\u00e4l\u00e4","year":"1993","unstructured":"\u00c4mm\u00e4l\u00e4 C, Eliasson L, Bokvist K, Larsson O, Ashcroft FM, Rorsman P (1993) Exocytosis elicited by action potentials and voltage-clamp calcium currents in individual mouse pancreatic B-cells. J Physiol (Lond) 472:665\u2013688","journal-title":"J Physiol (Lond)"},{"key":"1097_CR12","doi-asserted-by":"publisher","first-page":"1407","DOI":"10.1016\/0006-2952(82)90036-3","volume":"31","author":"Henquin","year":"1982","unstructured":"Henquin JC, Meissner HP (1982) Opposite effects of tolbutamide and diazoxide on 86Rb+ fluxes and membrane potential in pancreatic B cells. Biochem Pharmacol 31:1407\u20131415","journal-title":"Biochem Pharmacol"},{"key":"1097_CR13","doi-asserted-by":"crossref","first-page":"6712","DOI":"10.1073\/pnas.79.21.6712","volume":"79","author":"Neher","year":"1982","unstructured":"Neher E, Marty A (1982) Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells. Proc Natl Acad Sci USA 79:6712\u20136716","journal-title":"Proc Natl Acad Sci USA"},{"key":"1097_CR14","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1007\/BF00374568","volume":"428","author":"Carter","year":"1994","unstructured":"Carter TD, Ogden D (1994) Acetylcholine-stimulated changes of membrane potential and intracellular Ca2+ concentration recorded in endothelial cells in situ in the isolated rat aorta. Pflugers Arch 428:476\u2013484","journal-title":"Pflugers Arch"},{"key":"1097_CR15","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1113\/jphysiol.1978.sp012296","volume":"278","author":"I Atwater","year":"1978","unstructured":"Atwater I, Ribalet B, Rojas E (1978) Cyclic changes in potential and resistance of the beta-cell membrane induced by glucose in islets of Langerhans from mouse. J Physiol (Lond) 278:117\u2013139","journal-title":"J Physiol (Lond)"},{"key":"1097_CR16","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1042\/bj1320223","volume":"132","author":"Ashcroft","year":"1973","unstructured":"Ashcroft SJ, Weerasinghe LC, Randle PJ (1973) Interrelationship of islet metabolism, adenosine triphosphate content and insulin release. Biochem J 132:223\u2013231","journal-title":"Biochem J"},{"key":"1097_CR17","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/BF00582316","volume":"411","author":"Pusch","year":"1988","unstructured":"Pusch M, Neher E (1988) Rates of diffusional exchange between small cells and a measuring patch pipette. Pflugers Arch 411:204\u2013211","journal-title":"Pflugers Arch"},{"key":"1097_CR18","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/S0006-3495(90)82418-8","volume":"58","author":"Mathias","year":"1990","unstructured":"Mathias RT, Cohen IS, Oliva C (1990) Limitations of the whole cell patch clamp technique in the control of intracellular concentrations. Biophys J 58:759\u2013770","journal-title":"Biophys J"},{"key":"1097_CR19","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1002\/dmrr.329","volume":"18","author":"Straub","year":"2002","unstructured":"Straub SG, Sharp GW (2002) Glucose-stimulated signaling pathways in biphasic insulin secretion. Diabetes Metab Res Rev 18:451\u2013463","journal-title":"Diabetes Metab Res Rev"}],"container-title":["Pfl\u00fcgers Archiv"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00424-003-1097-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00424-003-1097-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00424-003-1097-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00424-003-1097-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T16:29:45Z","timestamp":1600273785000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00424-003-1097-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5,28]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["1097"],"URL":"https:\/\/doi.org\/10.1007\/s00424-003-1097-9","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1015135.195855","asserted-by":"object"}]},"ISSN":["0031-6768","1432-2013"],"issn-type":[{"value":"0031-6768","type":"print"},{"value":"1432-2013","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5,28]]},"assertion":[{"value":"21 January 2003","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 April 2003","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 May 2003","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}