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The present experiments were performed in order to characterize the ionic mechanisms underlying this response which has been thought to be due to Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    secretion. As there are regional differences within the caecal epithelium, the experiments were conducted at oral and aboral rat corpus caeci. In both caecal segments, the propionate-induced\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>sc<\/jats:sub>\n                    (\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Prop<\/jats:sub>\n                    ) was inhibited by\u2009&gt;\u200985%, when the experiments were performed either in nominally Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    - or nominally HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    -free buffer. In the case of Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    , the dependency was restricted to the presence of Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    in the serosal bath. Bumetanide, a blocker of the Na\n                    <jats:sup>+<\/jats:sup>\n                    -K\n                    <jats:sup>+<\/jats:sup>\n                    -2Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    -cotransporter, only numerically reduced\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Prop<\/jats:sub>\n                    suggesting that a large part of this current must be carried by an ion other than Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    . In the aboral caecum,\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Prop<\/jats:sub>\n                    was significantly inhibited by mucosally administered stilbene derivatives (SITS, DIDS, DNDS), which block anion exchangers. Serosal Na\n                    <jats:sup>+<\/jats:sup>\n                    -free buffer reduced I\n                    <jats:sub>Prop<\/jats:sub>\n                    significantly in the oral (and numerically also in aboral) corpus caeci. RT-PCR experiments revealed the expression of several forms of Na\n                    <jats:sup>+<\/jats:sup>\n                    -dependent HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    -cotransporters in caecum, which might underlie the observed Na\n                    <jats:sup>+<\/jats:sup>\n                    dependency. These results suggest that propionate sensing in caecum is coupled to HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2013<\/jats:sup>\n                    secretion, which functionally would stabilize luminal pH when the microbial fermentation leads to an increase in the concentration of short-chain fatty acids in the caecal lumen.\n                  <\/jats:p>","DOI":"10.1007\/s00424-021-02565-8","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T06:03:22Z","timestamp":1619676202000},"page":"937-951","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The role of HCO3\u2013 in propionate-induced anion secretion across rat caecal epithelium"],"prefix":"10.1007","volume":"473","author":[{"given":"Jasmin","family":"Ballout","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2194-4636","authenticated-orcid":false,"given":"Martin","family":"Diener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"2565_CR1","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1016\/j.mam.2012.07.009","volume":"34","author":"SL Alper","year":"2013","unstructured":"Alper SL, Sharma AK (2013) The SLC26 gene family of anion transporters and channels. 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