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Humanin (HN), a recently identified mitochondrial polypeptide, exhibits antioxidative and antiapoptotic properties. This work aimed to study the role of HN in a model of experimental intestinal I\/R injury and its effect on associated dysmotility. A total of 36 male adult albino rats were allocated into 3 equal groups. Sham group: merely a laparotomy was done. I\/R group: for 1\u00a0h, clamping of the superior mesenteric artery was done, and then reperfusion was allowed for 2\u00a0h later. HN-I\/R group: rats underwent ischemia and reperfusion, and 30\u00a0min before the reperfusion, they received an intraperitoneal injection of 252\u00a0\u03bcg\/kg of HN. Small intestinal motility was evaluated, and jejunal samples were got for biochemical and histological analysis. I\/R group showed elevation of intestinal NO, MDA, TNF- \u03b1, and IL-6 and decline of GPx and SOD levels. Furthermore, histologically, there were destructed jejunal villi especially their tips and increased tissue expression of caspase-3 and i-NOS, in addition to reduced small intestinal motility. Compared to I\/R group, HN-I\/R group exhibited decrease intestinal levels of NO, MDA, TNF- \u03b1, and IL-6 and increase GPx and SOD. Moreover, there was noticeable improvement of the histopathologic features and decreased caspase-3 and iNOS immunoreactivity, beside enhanced small intestinal motility. HN alleviates inflammation, apoptosis, and intestinal dysmotility encouraged by I\/R. Additionally, I\/R-induced apoptosis and motility alterations depend partly on the production of nitric oxide.<\/jats:p>","DOI":"10.1007\/s00424-023-02804-0","type":"journal-article","created":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T20:49:42Z","timestamp":1680727782000},"page":"655-666","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A novel beneficial role of humanin on intestinal apoptosis and dysmotility in a rat model of ischemia reperfusion injury"],"prefix":"10.1007","volume":"475","author":[{"given":"Eman R.","family":"Abozaid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8285-4496","authenticated-orcid":false,"given":"Reham H.","family":"Abdel-Kareem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa A.","family":"Habib","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,4,5]]},"reference":[{"key":"2804_CR1","doi-asserted-by":"publisher","first-page":"36","DOI":"10.5603\/FHC.2014.0004","volume":"52","author":"RA Akdogan","year":"2014","unstructured":"Akdogan RA, Kalkan Y, Tumkaya L, Alacam H, Erdivanli B, Aydin I (2014) Influence of infliximab pretreatment on ischemia\/reperfusion injury in rat intestine. 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