{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T15:14:37Z","timestamp":1778166877445,"version":"3.51.4"},"reference-count":61,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K10117"],"award-info":[{"award-number":["19K10117"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K10117"],"award-info":[{"award-number":["19K10117"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K10117"],"award-info":[{"award-number":["19K10117"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Promotion and Mutual Aid Corporation for Private School of Japan","award":["the Science Research Promotion Fund"],"award-info":[{"award-number":["the Science Research Promotion Fund"]}]},{"name":"the Promotion and Mutual Aid Corporation for Private School of Japan","award":["the Science Research Promotion Fund"],"award-info":[{"award-number":["the Science Research Promotion Fund"]}]},{"name":"the Promotion and Mutual Aid Corporation for Private School of Japan","award":["the Science Research Promotion Fund"],"award-info":[{"award-number":["the Science Research Promotion Fund"]}]},{"name":"Tokyo Dental College","award":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"],"award-info":[{"award-number":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"]}]},{"name":"Tokyo Dental College","award":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"],"award-info":[{"award-number":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"]}]},{"name":"Tokyo Dental College","award":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"],"award-info":[{"award-number":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"]}]},{"name":"Tokyo Dental College","award":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"],"award-info":[{"award-number":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"]}]},{"name":"Tokyo Dental College","award":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"],"award-info":[{"award-number":["the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial Functions)"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pflugers Arch - Eur J Physiol"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>In odontoblasts, intracellular Ca<jats:sup>2+<\/jats:sup> signaling plays key roles in reactionary dentin formation and generation of dentinal pain. Odontoblasts also express several G<jats:sub>s<\/jats:sub> protein-coupled receptors that promote production of cyclic AMP (cAMP). However, the crosstalk between intracellular cAMP and Ca<jats:sup>2+<\/jats:sup> signaling, as well as the role of cAMP in the cellular functions of odontoblasts, remains unclear. In this study, we measured intracellular cAMP levels and intracellular free Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>). We also investigated the effect of intracellular cAMP on mineralization by the odontoblasts. In the presence of extracellular Ca<jats:sup>2+<\/jats:sup>, the application of forskolin (adenylyl cyclase activator) or isoproterenol (G<jats:sub>s<\/jats:sub> protein-coupled beta-2 adrenergic receptor agonist) increased intracellular cAMP levels and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in odontoblasts. The [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increases could not be observed by removing extracellular Ca<jats:sup>2+<\/jats:sup>, indicating that cAMP is capable to activate Ca<jats:sup>2+<\/jats:sup> entry. Forskolin-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase was inhibited by a protein kinase A inhibitor in odontoblasts. The [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase was sensitive to Gd<jats:sup>3+<\/jats:sup>, 2APB, or Zn<jats:sup>2+<\/jats:sup> but not verapamil, ML218, or La<jats:sup>3+<\/jats:sup>. In immunofluorescence analyses, odontoblasts were immunopositive for calcium homeostasis modulator 1 (CALHM1), which was found close to ionotropic ATP receptor subtype, P2X<jats:sub>3<\/jats:sub> receptors. When CALHM1 was knocked down, forskolin-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase was suppressed. Alizarin red and von Kossa staining showed that forskolin decreased mineralization. These findings suggest that activation of adenylyl cyclase elicited increases in the intracellular cAMP level and Ca<jats:sup>2+<\/jats:sup> influx via protein kinase A activation in odontoblasts. Subsequent cAMP-dependent Ca<jats:sup>2+<\/jats:sup> influx was mediated by CALHM1 in odontoblasts. In addition, the intracellular cAMP signaling pathway in odontoblasts negatively mediated dentinogenesis.<\/jats:p>","DOI":"10.1007\/s00424-024-03038-4","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T03:24:35Z","timestamp":1731381875000},"page":"273-290","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Intracellular cAMP signaling-induced Ca2+ influx mediated by calcium homeostasis modulator 1 (CALHM1) in human odontoblasts"],"prefix":"10.1007","volume":"477","author":[{"given":"Maki","family":"Kimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachie","family":"Nomura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehito","family":"Ouchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuya","family":"Kurashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rei","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hinako","family":"Sekiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetaka","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyosuke","family":"Kono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Shibukawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,11,12]]},"reference":[{"key":"3038_CR1","doi-asserted-by":"publisher","first-page":"25556","DOI":"10.1074\/jbc.M002327200","volume":"275","author":"B Allard","year":"2000","unstructured":"Allard B, Couble ML, Magloire H, Bleicher F (2000) Characterization and gene expression of high conductance calcium-activated potassium channels displaying mechanosensitivity in human odontoblasts. J Biol Chem 275:25556\u201325561. https:\/\/doi.org\/10.1074\/jbc.M002327200","journal-title":"J Biol Chem"},{"key":"3038_CR2","doi-asserted-by":"publisher","first-page":"14860","DOI":"10.3390\/ijms232314860","volume":"23","author":"I Andriul\u0117","year":"2022","unstructured":"Andriul\u0117 I, Pangonyt\u0117 D, Gwanyanya A, Kar\u010diauskas D, Mubagwa K, Ma\u010dianskien\u0117 R (2022) Detection of TRPM6 and TRPM7 proteins in normal and diseased cardiac atrial tissue and isolated cardiomyocytes. Int J Mol Sci 23:14860. https:\/\/doi.org\/10.3390\/ijms232314860","journal-title":"Int J Mol Sci"},{"key":"3038_CR3","doi-asserted-by":"publisher","first-page":"C515","DOI":"10.1152\/ajpcell.1990.259.3.C515","volume":"259","author":"BA Biagi","year":"1990","unstructured":"Biagi BA, Enyeart JJ (1990) Gadolinium blocks low- and high-threshold calcium currents in pituitary cells. Am J Physiol 259:C515-520. https:\/\/doi.org\/10.1152\/ajpcell.1990.259.3.C515","journal-title":"Am J Physiol"},{"key":"3038_CR4","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1124\/pr.57.4.6","volume":"57","author":"DE Clapham","year":"2005","unstructured":"Clapham DE, Julius D, Montell C, Schultz G (2005) International union of pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels. Pharmacol Rev 57:427\u2013450. https:\/\/doi.org\/10.1124\/pr.57.4.6","journal-title":"Pharmacol Rev"},{"key":"3038_CR5","doi-asserted-by":"publisher","first-page":"e14781","DOI":"10.14814\/phy2.14781","volume":"9","author":"J Dolinina","year":"2021","unstructured":"Dolinina J, Rippe A, \u00d6berg CM (2021) Clemizole and La3+ salts ameliorate angiotensin II-induced glomerular hyperpermeability in vivo. Physiol Rep 9:e14781. https:\/\/doi.org\/10.14814\/phy2.14781","journal-title":"Physiol Rep"},{"key":"3038_CR6","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/j.ejphar.2015.05.016","volume":"763","author":"NM Duc","year":"2015","unstructured":"Duc NM, Kim HR, Chung KY (2015) Structural mechanism of G protein activation by G protein-coupled receptor. Eur J Pharmacol 763:214\u2013222. https:\/\/doi.org\/10.1016\/j.ejphar.2015.05.016","journal-title":"Eur J Pharmacol"},{"key":"3038_CR7","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1177\/0022034514544507","volume":"93","author":"O Egbuniwe","year":"2014","unstructured":"Egbuniwe O, Grover S, Duggal AK, Mavroudis A, Yazdi M, Renton T, Di Silvio L, Grant AD (2014) TRPA1 and TRPV4 activation in human odontoblasts stimulates ATP release. J Dent Res 93:911\u2013917. https:\/\/doi.org\/10.1177\/0022034514544507","journal-title":"J Dent Res"},{"key":"3038_CR8","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1111\/j.1600-0722.2006.00343.x","volume":"114","author":"IA El Karim","year":"2006","unstructured":"El Karim IA, Lamey P-J, Linden GJ, Awawdeh LA, Lundy FT (2006) Caries-induced changes in the expression of pulpal neuropeptide Y. Eur J Oral Sci 114:133\u2013137. https:\/\/doi.org\/10.1111\/j.1600-0722.2006.00343.x","journal-title":"Eur J Oral Sci"},{"key":"3038_CR9","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.neures.2006.09.016","volume":"57","author":"SH Gautam","year":"2007","unstructured":"Gautam SH, Otsuguro K-I, Ito S, Saito T, Habara Y (2007) T-type Ca2+ channels contribute to IBMX\/forskolin- and K+-induced Ca2+ transients in porcine olfactory receptor neurons. Neurosci Res 57:129\u2013139. https:\/\/doi.org\/10.1016\/j.neures.2006.09.016","journal-title":"Neurosci Res"},{"key":"3038_CR10","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.1016\/j.joen.2015.01.010","volume":"41","author":"J Gu","year":"2015","unstructured":"Gu J, Ikeda H, Suda H (2015) Sympathetic regulation of tertiary dentinogenesis via beta-2 adrenergic receptor on rat odontoblasts. J Endod 41:1056\u20131060. https:\/\/doi.org\/10.1016\/j.joen.2015.01.010","journal-title":"J Endod"},{"key":"3038_CR11","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1359\/jbmr.1999.14.8.1357","volume":"14","author":"L Guo","year":"1999","unstructured":"Guo L, Davidson RM (1999) Extracellular Ca2+ increases cytosolic free Ca2+ in freshly isolated rat odontoblasts. J Bone Miner Res Off J Am Soc Bone Miner Res 14:1357\u20131366. https:\/\/doi.org\/10.1359\/jbmr.1999.14.8.1357","journal-title":"J Bone Miner Res Off J Am Soc Bone Miner Res"},{"key":"3038_CR12","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1016\/s0306-4522(03)00269-0","volume":"120","author":"SR Haug","year":"2003","unstructured":"Haug SR, Heyeraas KJ (2003) Effects of sympathectomy on experimentally induced pulpal inflammation and periapical lesions in rats. Neuroscience 120:827\u2013836. https:\/\/doi.org\/10.1016\/s0306-4522(03)00269-0","journal-title":"Neuroscience"},{"key":"3038_CR13","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1111\/j.1365-2591.1993.tb00537.x","volume":"26","author":"KJ Heyeraas","year":"1993","unstructured":"Heyeraas KJ (1993) Micropuncture measurements of interstitial fluid and vascular pressures in dental pulp. Int Endod J 26:15\u201316. https:\/\/doi.org\/10.1111\/j.1365-2591.1993.tb00537.x","journal-title":"Int Endod J"},{"key":"3038_CR14","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1016\/j.joen.2012.06.015","volume":"38","author":"H Ichikawa","year":"2012","unstructured":"Ichikawa H, Kim H-J, Shuprisha A, Shikano T, Tsumura M, Shibukawa Y, Tazaki M (2012) Voltage-dependent sodium channels and calcium-activated potassium channels in human odontoblasts in vitro. J Endod 38:1355\u20131362. https:\/\/doi.org\/10.1016\/j.joen.2012.06.015","journal-title":"J Endod"},{"key":"3038_CR15","doi-asserted-by":"publisher","first-page":"3562","DOI":"10.1074\/jbc.M211484200","volume":"278","author":"S Jung","year":"2003","unstructured":"Jung S, M\u00fchle A, Schaefer M, Strotmann R, Schultz G, Plant TD (2003) Lanthanides potentiate TRPC5 currents by an action at extracellular sites close to the pore mouth. J Biol Chem 278:3562\u20133571. https:\/\/doi.org\/10.1074\/jbc.M211484200","journal-title":"J Biol Chem"},{"key":"3038_CR16","doi-asserted-by":"publisher","first-page":"5799","DOI":"10.3748\/wjg.15.5799","volume":"15","author":"BJ Kim","year":"2009","unstructured":"Kim BJ, Park KJ, Kim HW, Choi S, Jun JY, Chang IY, Jeon J-H, So I, Kim SJ (2009) Identification of TRPM7 channels in human intestinal interstitial cells of Cajal. World J Gastroenterol 15:5799\u20135804. https:\/\/doi.org\/10.3748\/wjg.15.5799","journal-title":"World J Gastroenterol"},{"key":"3038_CR17","doi-asserted-by":"publisher","first-page":"104864","DOI":"10.1016\/j.archoralbio.2020.104864","volume":"118","author":"JH Kim","year":"2020","unstructured":"Kim JH, Won J, Oh SB (2020) Expression of CaV3.1 T-type calcium channels in acutely isolated adult rat odontoblasts. Arch Oral Biol 118:104864. https:\/\/doi.org\/10.1016\/j.archoralbio.2020.104864","journal-title":"Arch Oral Biol"},{"key":"3038_CR18","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.3390\/biom11071010","volume":"11","author":"M Kimura","year":"2021","unstructured":"Kimura M, Mochizuki H, Satou R, Iwasaki M, Kokubu E, Kono K, Nomura S, Sakurai T, Kuroda H, Shibukawa Y (2021) Plasma membrane Ca2+-ATPase in rat and human odontoblasts mediates dentin mineralization. Biomolecules 11:1010. https:\/\/doi.org\/10.3390\/biom11071010","journal-title":"Biomolecules"},{"key":"3038_CR19","doi-asserted-by":"publisher","first-page":"443","DOI":"10.3389\/fphys.2018.00443","volume":"9","author":"M Kimura","year":"2018","unstructured":"Kimura M, Nishi K, Higashikawa A, Ohyama S, Sakurai K, Tazaki M, Shibukawa Y (2018) High pH-sensitive store-operated Ca2+ entry mediated by Ca2+ release-activated Ca2+ channels in rat odontoblasts. Front Physiol 9:443. https:\/\/doi.org\/10.3389\/fphys.2018.00443","journal-title":"Front Physiol"},{"key":"3038_CR20","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1177\/0022034516644702","volume":"95","author":"M Kimura","year":"2016","unstructured":"Kimura M, Sase T, Higashikawa A, Sato M, Sato T, Tazaki M, Shibukawa Y (2016) High pH-sensitive TRPA1 activation in odontoblasts regulates mineralization. J Dent Res 95:1057\u20131064. https:\/\/doi.org\/10.1177\/0022034516644702","journal-title":"J Dent Res"},{"key":"3038_CR21","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1016\/j.archoralbio.2007.02.006","volume":"52","author":"M Kitagawa","year":"2007","unstructured":"Kitagawa M, Ueda H, Iizuka S, Sakamoto K, Oka H, Kudo Y, Ogawa I, Miyauchi M, Tahara H, Takata T (2007) Immortalization and characterization of human dental pulp cells with odontoblastic differentiation. Arch Oral Biol 52:727\u2013731. https:\/\/doi.org\/10.1016\/j.archoralbio.2007.02.006","journal-title":"Arch Oral Biol"},{"key":"3038_CR22","doi-asserted-by":"publisher","first-page":"879","DOI":"10.3390\/biom13060879","volume":"13","author":"E Kitayama","year":"2023","unstructured":"Kitayama E, Kimura M, Ouchi T, Furusawa M, Shibukawa Y (2023) Functional expression of IP, 5-HT4, D1, A2A, and VIP receptors in human odontoblast cell line. Biomolecules 13:879. https:\/\/doi.org\/10.3390\/biom13060879","journal-title":"Biomolecules"},{"key":"3038_CR23","doi-asserted-by":"publisher","first-page":"1078","DOI":"10.3389\/fphys.2017.01078","volume":"8","author":"Y Kojima","year":"2017","unstructured":"Kojima Y, Kimura M, Higashikawa A, Kono K, Ando M, Tazaki M, Shibukawa Y (2017) Potassium currents activated by depolarization in odontoblasts. Front Physiol 8:1078. https:\/\/doi.org\/10.3389\/fphys.2017.01078","journal-title":"Front Physiol"},{"key":"3038_CR24","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1097\/WNR.0b013e3283568a29","volume":"23","author":"H Kuroda","year":"2012","unstructured":"Kuroda H, Shibukawa Y, Soya M, Masamura A, Kasahara M, Tazaki M, Ichinohe T (2012) Expression of P2X\u2081 and P2X\u2084 receptors in rat trigeminal ganglion neurons. NeuroReport 23:752\u2013756. https:\/\/doi.org\/10.1097\/WNR.0b013e3283568a29","journal-title":"NeuroReport"},{"issue":"Suppl 1","key":"3038_CR25","first-page":"1","volume":"24","author":"L Lacinov\u00e1","year":"2005","unstructured":"Lacinov\u00e1 L (2005) Voltage-dependent calcium channels. Gen Physiol Biophys 24(Suppl 1):1\u201378","journal-title":"Gen Physiol Biophys"},{"key":"3038_CR26","doi-asserted-by":"publisher","first-page":"403","DOI":"10.4149\/gpb_2011_04_403","volume":"30","author":"L Lacinov\u00e1","year":"2011","unstructured":"Lacinov\u00e1 L (2011) T-type calcium channel blockers - new and notable. Gen Physiol Biophys 30:403\u2013409. https:\/\/doi.org\/10.4149\/gpb_2011_04_403","journal-title":"Gen Physiol Biophys"},{"key":"3038_CR27","doi-asserted-by":"publisher","first-page":"155","DOI":"10.3389\/fncel.2013.00155","volume":"7","author":"G Lepski","year":"2013","unstructured":"Lepski G, Jannes CE, Nikkhah G, Bischofberger J (2013) cAMP promotes the differentiation of neural progenitor cells in vitro via modulation of voltage-gated calcium channels. Front Cell Neurosci 7:155. https:\/\/doi.org\/10.3389\/fncel.2013.00155","journal-title":"Front Cell Neurosci"},{"key":"3038_CR28","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1161\/CIRCRESAHA.115.306529","volume":"118","author":"F Lezoualc\u2019h","year":"2016","unstructured":"Lezoualc\u2019h F, Fazal L, Laudette M, Conte C (2016) Cyclic AMP sensor EPAC proteins and their role in cardiovascular function and disease. Circ Res 118:881\u2013897. https:\/\/doi.org\/10.1161\/CIRCRESAHA.115.306529","journal-title":"Circ Res"},{"key":"3038_CR29","doi-asserted-by":"publisher","first-page":"1259","DOI":"10.1177\/0022034515592858","volume":"94","author":"X Liu","year":"2015","unstructured":"Liu X, Wang C, Fujita T, Malmstrom HS, Nedergaard M, Ren YF, Dirksen RT (2015) External dentin stimulation induces ATP release in human teeth. J Dent Res 94:1259\u20131266. https:\/\/doi.org\/10.1177\/0022034515592858","journal-title":"J Dent Res"},{"key":"3038_CR30","doi-asserted-by":"publisher","first-page":"E1963","DOI":"10.1073\/pnas.1204023109","volume":"109","author":"Z Ma","year":"2012","unstructured":"Ma Z, Siebert AP, Cheung K-H, Lee RJ, Johnson B, Cohen AS, Vingtdeux V, Marambaud P, Foskett JK (2012) Calcium homeostasis modulator 1 (CALHM1) is the pore-forming subunit of an ion channel that mediates extracellular Ca2+ regulation of neuronal excitability. Proc Natl Acad Sci U S A 109:E1963-1971. https:\/\/doi.org\/10.1073\/pnas.1204023109","journal-title":"Proc Natl Acad Sci U S A"},{"key":"3038_CR31","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1007\/s00424-015-1757-6","volume":"468","author":"Z Ma","year":"2016","unstructured":"Ma Z, Tanis JE, Taruno A, Foskett JK (2016) Calcium homeostasis modulator (CALHM) ion channels. Pflugers Arch 468:395\u2013403. https:\/\/doi.org\/10.1007\/s00424-015-1757-6","journal-title":"Pflugers Arch"},{"key":"3038_CR32","doi-asserted-by":"publisher","first-page":"704518","DOI":"10.3389\/fphys.2021.704518","volume":"12","author":"M Matsunaga","year":"2021","unstructured":"Matsunaga M, Kimura M, Ouchi T, Nakamura T, Ohyama S, Ando M, Nomura S, Azuma T, Ichinohe T, Shibukawa Y (2021) Mechanical stimulation-induced calcium signaling by Piezo1 channel activation in human odontoblast reduces dentin mineralization. Front Physiol 12:704518. https:\/\/doi.org\/10.3389\/fphys.2021.704518","journal-title":"Front Physiol"},{"key":"3038_CR33","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1113\/jphysiol.1993.sp019835","volume":"469","author":"B Mlinar","year":"1993","unstructured":"Mlinar B, Enyeart JJ (1993) Block of current through T-type calcium channels by trivalent metal cations and nickel in neural rat and human cells. J Physiol 469:639\u2013652. https:\/\/doi.org\/10.1113\/jphysiol.1993.sp019835","journal-title":"J Physiol"},{"key":"3038_CR34","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1085\/jgp.20028740","volume":"121","author":"MK Monteilh-Zoller","year":"2003","unstructured":"Monteilh-Zoller MK, Hermosura MC, Nadler MJS, Scharenberg AM, Penner R, Fleig A (2003) TRPM7 provides an ion channel mechanism for cellular entry of trace metal ions. J Gen Physiol 121:49\u201360. https:\/\/doi.org\/10.1085\/jgp.20028740","journal-title":"J Gen Physiol"},{"key":"3038_CR35","doi-asserted-by":"publisher","first-page":"258","DOI":"10.3389\/fphys.2016.00258","volume":"7","author":"Y Nakano","year":"2016","unstructured":"Nakano Y, Le MH, Abduweli D, Ho SP, Ryazanova LV, Hu Z, Ryazanov AG, Den Besten PK, Zhang Y (2016) A critical role of TRPM7 as an ion channel protein in mediating the mineralization of the craniofacial hard tissues. Front Physiol 7:258. https:\/\/doi.org\/10.3389\/fphys.2016.00258","journal-title":"Front Physiol"},{"key":"3038_CR36","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.ceca.2016.07.003","volume":"60","author":"A Nishiyama","year":"2016","unstructured":"Nishiyama A, Sato M, Kimura M, Katakura A, Tazaki M, Shibukawa Y (2016) Intercellular signal communication among odontoblasts and trigeminal ganglion neurons via glutamate. Cell Calcium 60:341\u2013355. https:\/\/doi.org\/10.1016\/j.ceca.2016.07.003","journal-title":"Cell Calcium"},{"key":"3038_CR37","doi-asserted-by":"publisher","first-page":"91759","DOI":"10.3389\/fphys.2022.891759","volume":"13","author":"S Ohyama","year":"2022","unstructured":"Ohyama S, Ouchi T, Kimura M, Kurashima R, Yasumatsu K, Nishida D, Hitomi S, Ubaidus S, Kuroda H, Ito S, Takano M, Ono K, Mizoguchi T, Katakura A, Shibukawa Y (2022) Piezo1-pannexin-1-P2X3 axis in odontoblasts and neurons mediates sensory transduction in dentinal sensitivity. Front Physiol 13:91759. https:\/\/doi.org\/10.3389\/fphys.2022.891759","journal-title":"Front Physiol"},{"key":"3038_CR38","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1113\/jphysiol.1991.sp018746","volume":"441","author":"DB Reichling","year":"1991","unstructured":"Reichling DB, MacDermott AB (1991) Lanthanum actions on excitatory amino acid-gated currents and voltage-gated calcium currents in rat dorsal horn neurons. J Physiol 441:199\u2013218. https:\/\/doi.org\/10.1113\/jphysiol.1991.sp018746","journal-title":"J Physiol"},{"key":"3038_CR39","doi-asserted-by":"publisher","first-page":"1657","DOI":"10.1113\/jphysiol.2009.170555","volume":"587","author":"CD Roberts","year":"2009","unstructured":"Roberts CD, Dvoryanchikov G, Roper SD, Chaudhari N (2009) Interaction between the second messengers cAMP and Ca2+ in mouse presynaptic taste cells. J Physiol 587:1657\u20131668. https:\/\/doi.org\/10.1113\/jphysiol.2009.170555","journal-title":"J Physiol"},{"key":"3038_CR40","doi-asserted-by":"publisher","unstructured":"Rychkov G, Barritt GJ (2007) TRPC1 Ca2+-permeable channels in animal cells. Handb Exp Pharmacol 23\u201352. https:\/\/doi.org\/10.1007\/978-3-540-34891-7_2","DOI":"10.1007\/978-3-540-34891-7_2"},{"key":"3038_CR41","doi-asserted-by":"publisher","first-page":"1747","DOI":"10.3390\/biom12121747","volume":"12","author":"N Saito","year":"2022","unstructured":"Saito N, Kimura M, Ouchi T, Ichinohe T, Shibukawa Y (2022) G\u03b1s-coupled CGRP receptor signaling axis from the trigeminal ganglion neuron to odontoblast negatively regulates dentin mineralization. Biomolecules 12:1747. https:\/\/doi.org\/10.3390\/biom12121747","journal-title":"Biomolecules"},{"key":"3038_CR42","doi-asserted-by":"publisher","first-page":"326","DOI":"10.3389\/fphys.2015.00326","volume":"6","author":"M Sato","year":"2015","unstructured":"Sato M, Furuya T, Kimura M, Kojima Y, Tazaki M, Sato T, Shibukawa Y (2015) Intercellular odontoblast communication via ATP mediated by Pannexin-1 channel and phospholipase C-coupled receptor activation. Front Physiol 6:326. https:\/\/doi.org\/10.3389\/fphys.2015.00326","journal-title":"Front Physiol"},{"key":"3038_CR43","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1016\/j.joen.2018.02.020","volume":"44","author":"M Sato","year":"2018","unstructured":"Sato M, Ogura K, Kimura M, Nishi K, Ando M, Tazaki M, Shibukawa Y (2018) Activation of mechanosensitive transient receptor potential\/Piezo channels in odontoblasts generates action potentials in cocultured isolectin B4-negative medium-sized trigeminal ganglion neurons. J Endod 44:984-991.e2. https:\/\/doi.org\/10.1016\/j.joen.2018.02.020","journal-title":"J Endod"},{"key":"3038_CR44","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1016\/j.joen.2013.01.012","volume":"39","author":"M Sato","year":"2013","unstructured":"Sato M, Sobhan U, Tsumura M, Kuroda H, Soya M, Masamura A, Nishiyama A, Katakura A, Ichinohe T, Tazaki M, Shibukawa Y (2013) Hypotonic-induced stretching of plasma membrane activates transient receptor potential vanilloid channels and sodium-calcium exchangers in mouse odontoblasts. J Endod 39:779\u2013787. https:\/\/doi.org\/10.1016\/j.joen.2013.01.012","journal-title":"J Endod"},{"key":"3038_CR45","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1007\/s00424-014-1551-x","volume":"467","author":"Y Shibukawa","year":"2015","unstructured":"Shibukawa Y, Sato M, Kimura M, Sobhan U, Shimada M, Nishiyama A, Kawaguchi A, Soya M, Kuroda H, Katakura A, Ichinohe T, Tazaki M (2015) Odontoblasts as sensory receptors: transient receptor potential channels, pannexin-1, and ionotropic ATP receptors mediate intercellular odontoblast-neuron signal transduction. Pfl\u00fcg Arch Eur J Physiol 467:843\u2013863. https:\/\/doi.org\/10.1007\/s00424-014-1551-x","journal-title":"Pfl\u00fcg Arch Eur J Physiol"},{"key":"3038_CR46","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1359\/jbmr.2003.18.1.30","volume":"18","author":"Y Shibukawa","year":"2003","unstructured":"Shibukawa Y, Suzuki T (2003) Ca2+ signaling mediated by IP3-dependent Ca2+ releasing and store-operated Ca2+ channels in rat odontoblasts. J Bone Miner Res Off J Am Soc Bone Miner Res 18:30\u201338. https:\/\/doi.org\/10.1359\/jbmr.2003.18.1.30","journal-title":"J Bone Miner Res Off J Am Soc Bone Miner Res"},{"key":"3038_CR47","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.neulet.2008.02.015","volume":"435","author":"Y Shimeno","year":"2008","unstructured":"Shimeno Y, Sugawara Y, Iikubo M, Shoji N, Sasano T (2008) Sympathetic nerve fibers sprout into rat odontoblast layer, but not into dentinal tubules, in response to cavity preparation. Neurosci Lett 435:73\u201377. https:\/\/doi.org\/10.1016\/j.neulet.2008.02.015","journal-title":"Neurosci Lett"},{"key":"3038_CR48","doi-asserted-by":"publisher","first-page":"3","DOI":"10.3389\/fphys.2017.00003","volume":"8","author":"Y Shiozaki","year":"2017","unstructured":"Shiozaki Y, Sato M, Kimura M, Sato T, Tazaki M, Shibukawa Y (2017) Ionotropic P2X ATP receptor channels mediate purinergic signaling in mouse odontoblasts. Front Physiol 8:3. https:\/\/doi.org\/10.3389\/fphys.2017.00003","journal-title":"Front Physiol"},{"key":"3038_CR49","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/j.joen.2016.10.021","volume":"43","author":"Z Song","year":"2017","unstructured":"Song Z, Chen L, Guo J, Qin W, Wang R, Huang S, Yang X, Tian Y, Lin Z (2017) The role of transient receptor potential cation channel, subfamily C, member 1 in the odontoblast-like differentiation of human dental pulp cells. J Endod 43:315\u2013320. https:\/\/doi.org\/10.1016\/j.joen.2016.10.021","journal-title":"J Endod"},{"key":"3038_CR50","doi-asserted-by":"publisher","first-page":"3850","DOI":"10.1016\/j.jmb.2016.08.002","volume":"428","author":"V Syrovatkina","year":"2016","unstructured":"Syrovatkina V, Alegre KO, Dey R, Huang X-Y (2016) Regulation, signaling, and physiological functions of G-proteins. J Mol Biol 428:3850\u20133868. https:\/\/doi.org\/10.1016\/j.jmb.2016.08.002","journal-title":"J Mol Biol"},{"key":"3038_CR51","doi-asserted-by":"publisher","first-page":"808","DOI":"10.3390\/ijms19030808","volume":"19","author":"A Taruno","year":"2018","unstructured":"Taruno A (2018) ATP release channels. Int J Mol Sci 19:808. https:\/\/doi.org\/10.3390\/ijms19030808","journal-title":"Int J Mol Sci"},{"key":"3038_CR52","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s00424-020-02464-4","volume":"473","author":"A Taruno","year":"2021","unstructured":"Taruno A, Nomura K, Kusakizako T, Ma Z, Nureki O, Foskett JK (2021) Taste transduction and channel synapses in taste buds. Pflugers Arch 473:3\u201313. https:\/\/doi.org\/10.1007\/s00424-020-02464-4","journal-title":"Pflugers Arch"},{"key":"3038_CR53","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1016\/j.joen.2010.01.006","volume":"36","author":"M Tsumura","year":"2010","unstructured":"Tsumura M, Okumura R, Tatsuyama S, Ichikawa H, Muramatsu T, Matsuda T, Baba A, Suzuki K, Kajiya H, Sahara Y, Tokuda M, Momose Y, Tazaki M, Shimono M, Shibukawa Y (2010) Ca2+ extrusion via Na+-Ca2+ exchangers in rat odontoblasts. J Endod 36:668\u2013674. https:\/\/doi.org\/10.1016\/j.joen.2010.01.006","journal-title":"J Endod"},{"key":"3038_CR54","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.ceca.2012.05.002","volume":"52","author":"M Tsumura","year":"2012","unstructured":"Tsumura M, Sobhan U, Muramatsu T, Sato M, Ichikawa H, Sahara Y, Tazaki M, Shibukawa Y (2012) TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium\u2013calcium exchangers in rat odontoblasts. Cell Calcium 52:124\u2013136. https:\/\/doi.org\/10.1016\/j.ceca.2012.05.002","journal-title":"Cell Calcium"},{"key":"3038_CR55","doi-asserted-by":"publisher","first-page":"e82233","DOI":"10.1371\/journal.pone.0082233","volume":"8","author":"M Tsumura","year":"2013","unstructured":"Tsumura M, Sobhan U, Sato M, Shimada M, Nishiyama A, Kawaguchi A, Soya M, Kuroda H, Tazaki M, Shibukawa Y (2013) Functional expression of TRPM8 and TRPA1 channels in rat odontoblasts. PLoS ONE 8:e82233. https:\/\/doi.org\/10.1371\/journal.pone.0082233","journal-title":"PLoS ONE"},{"key":"3038_CR56","doi-asserted-by":"publisher","first-page":"5767","DOI":"10.1167\/iovs.07-0412","volume":"48","author":"S Wimmers","year":"2007","unstructured":"Wimmers S, Strauss O (2007) Basal calcium entry in retinal pigment epithelial cells is mediated by TRPC channels. Invest Ophthalmol Vis Sci 48:5767\u20135772. https:\/\/doi.org\/10.1167\/iovs.07-0412","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"3038_CR57","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.archoralbio.2018.09.011","volume":"96","author":"J Won","year":"2018","unstructured":"Won J, Kim JH, Oh SB (2018) Molecular expression of Mg2+ regulator TRPM7 and CNNM4 in rat odontoblasts. Arch Oral Biol 96:182\u2013188. https:\/\/doi.org\/10.1016\/j.archoralbio.2018.09.011","journal-title":"Arch Oral Biol"},{"key":"3038_CR58","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1038\/sj.bjp.0706197","volume":"145","author":"S-Z Xu","year":"2005","unstructured":"Xu S-Z, Zeng F, Boulay G, Grimm C, Harteneck C, Beech DJ (2005) Block of TRPC5 channels by 2-aminoethoxydiphenyl borate: a differential, extracellular and voltage-dependent effect. Br J Pharmacol 145:405\u2013414. https:\/\/doi.org\/10.1038\/sj.bjp.0706197","journal-title":"Br J Pharmacol"},{"key":"3038_CR59","doi-asserted-by":"publisher","first-page":"73","DOI":"10.3892\/etm.2017.4471","volume":"14","author":"X Yang","year":"2017","unstructured":"Yang X, Song Z, Chen L, Wang R, Huang S, Qin W, Guo J, Lin Z (2017) Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells. Exp Ther Med 14:73\u201378. https:\/\/doi.org\/10.3892\/etm.2017.4471","journal-title":"Exp Ther Med"},{"key":"3038_CR60","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1124\/pharmrev.120.000086","volume":"73","author":"M Zaccolo","year":"2021","unstructured":"Zaccolo M, Zerio A, Lobo MJ (2021) Subcellular organization of the cAMP signaling pathway. Pharmacol Rev 73:278\u2013309. https:\/\/doi.org\/10.1124\/pharmrev.120.000086","journal-title":"Pharmacol Rev"},{"key":"3038_CR61","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1111\/iej.13400","volume":"54","author":"C Zhan","year":"2021","unstructured":"Zhan C, Huang M, Yang X, Hou J (2021) Dental nerves: a neglected mediator of pulpitis. Int Endod J 54:85\u201399. https:\/\/doi.org\/10.1111\/iej.13400","journal-title":"Int Endod J"}],"container-title":["Pfl\u00fcgers Archiv - European Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00424-024-03038-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00424-024-03038-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00424-024-03038-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T05:50:03Z","timestamp":1737697803000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00424-024-03038-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,12]]},"references-count":61,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["3038"],"URL":"https:\/\/doi.org\/10.1007\/s00424-024-03038-4","relation":{},"ISSN":["0031-6768","1432-2013"],"issn-type":[{"value":"0031-6768","type":"print"},{"value":"1432-2013","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,12]]},"assertion":[{"value":"4 January 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 August 2024","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2024","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 November 2024","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}