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Rho-kinases (ROCK) are known to be important for stable changes in synaptic strength, especially LTP. In this study, we investigated whether LTP inhibition in synapses primed with 1-Hz stimulation was affected by ROCK inhibition in young adult male rats. The study also examined the pattern of tau phosphorylation that occurs during metaplastic regulation, applying into perspective the phosphorylation of tau protein by ROCK. Field potentials consisting of an excitatory postsynaptic potential (fEPSP) and population spike (PS) were recorded from the granule cell layer of the hippocampal dentate gyrus (DG). Metaplastic LTP was induced by strong tetanic stimulation (HFS) of the lateral perforant path after a low-frequency stimulation (LFS) protocol. A glass micropipette was inserted into the granule cell layer of the ipsilateral dentate gyrus to record fEPSP and drug infusion. Drug infusion (saline, <jats:italic>n<\/jats:italic>\u2009=\u20098; fasudil, <jats:italic>n<\/jats:italic>\u2009=\u20098, 10\u00a0\u00b5M) was started after the 15-min baseline recording and lasted for 60\u00a0min. Total and phosphorylated tau levels were measured in the stimulated hippocampus, which was immediately removed after the electrophysiological recording. LFS prevented the induction of LTP in response to HFS and even produced synaptic LTD in the saline-infused group (83.8\u2009\u00b1\u20092.6% of the baseline), but moderate potentiation of fEPSP (121.1\u2009\u00b1\u20097.7% of the baseline) occurred at the end of recording in the experiments where fasudil infusion was performed. LFS caused a comparable early depression, and HFS resulted in a comparable potentiation of the PS amplitude in both groups. Granular cells of the DG failed to exhibit synaptic LTP inhibition in the presence of fasudil, and levels of total and phosphorylated GSK-3\u03b2 and levels of phosphorylated tau (Ser<jats:sup>396<\/jats:sup> and Ser<jats:sup>202<\/jats:sup>-Thr<jats:sup>205<\/jats:sup>) were found to be lower than those of the control group. Based on these findings, it can be concluded that pharmacological inhibition of ROCK results in impaired ability of dentate gyrus neurons to inhibit synaptic LTP, and this result is accompanied by decreased phosphorylation of GSK-3\u03b2 and tau proteins. The negative effect of fasudil on neuronal function should not be neglected when evaluating its effects as a therapeutic agent for diseases.<\/jats:p>","DOI":"10.1007\/s00424-025-03078-4","type":"journal-article","created":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T02:41:06Z","timestamp":1744425666000},"page":"787-796","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Inhibition of Rho-kinase by fasudil contributes to the modulation of the synaptic plasticity response in the rat hippocampus"],"prefix":"10.1007","volume":"477","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1445-6423","authenticated-orcid":false,"given":"Ercan","family":"Babur","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8361-0618","authenticated-orcid":false,"given":"Hatice","family":"Saray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6455-6644","authenticated-orcid":false,"given":"Cem","family":"S\u00fcer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7560-216X","authenticated-orcid":false,"given":"Nurcan","family":"Dursun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,12]]},"reference":[{"issue":"4","key":"3078_CR1","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1016\/S0166-2236(96)80018-X","volume":"19","author":"WC Abraham","year":"1996","unstructured":"Abraham WC, Bear MF (1996) Metaplasticity: the plasticity of synaptic plasticity. 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