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However, it remains unclear whether increased loading also affects body weight through acute changes in extracellular water. This study aimed to determine whether increased weight-bearing load acutely produces negative sodium and water balances, as indicators of changes in whole-body extracellular sodium and water content. Diet-induced obese (DIO) rats were housed in metabolic cages with free access to hypotonic 0.5% NaCl solution and a low-sodium, high-fat diet. A novel, less traumatic loading method was used, where intra-abdominal capsules implanted two weeks earlier were either filled in vivo with wolfram granulate (Load group) or sham-filled (Control group), resulting in 15% and 2% increases in body weight, respectively. Compared to controls, increased weight-bearing load decreased body weight by 4.2% (95% CI -6.3, -2.0; <jats:italic>P<\/jats:italic>\u2009=\u20090.001) and reduced food intake by 1.6 percentage points (95% CI -2.6, -0.6; <jats:italic>P<\/jats:italic>\u2009=\u20090.003). No significant differences in sodium or water balance were observed. These findings suggest that load-induced reductions in body weight are not mediated by changes in whole-body extracellular sodium or water content, indicating that fluid homeostasis does not contribute to the homeostatic regulation of body weight.<\/jats:p>","DOI":"10.1007\/s00424-025-03114-3","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T01:25:58Z","timestamp":1756776358000},"page":"1287-1294","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Increased weight-bearing load reduces biological body weight while sodium and water balances are unaffected"],"prefix":"10.1007","volume":"477","author":[{"given":"Jovana","family":"Zlatkovic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakob","family":"Bellman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"H\u00e4gg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathilda","family":"Magnusson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claes","family":"Ohlsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald","family":"DiBona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fredrik","family":"Anesten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John-Olov","family":"Jansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"issue":"1","key":"3114_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1056\/NEJMoa1614362","volume":"377","author":"A Afshin","year":"2017","unstructured":"Afshin A, Forouzanfar MH, Reitsma MB, Sur P, Estep K, Lee A et al (2017) Health effects of overweight and obesity in 195 countries over 25 years. 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