{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T07:27:43Z","timestamp":1786692463089,"version":"build-2736575974"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universit\u00e4tsklinikum Hamburg-Eppendorf (UKE)"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pflugers Arch - Eur J Physiol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The kidney slit diaphragm (SD) connects the foot processes of glomerular podocytes, anatomically forming the outermost part of the three-layered glomerular filtration barrier. The SD is a large molecular polymer that, together with the foot processes, covers the glomerular capillaries and has diverse functions. It establishes a strong yet flexible cell-cell contact between foot processes from neighboring podocytes and additionally acts as a dynamic signaling platform linking structural integrity with cellular responses. Understanding the physiology of the SD has been hampered by limited structural insights and a lack of precise knowledge of protein composition of its building blocks.<\/jats:p>\n                  <jats:p>The recent convergence of the proteomic analyses of the SD with atomically resolved structures and in situ cryo-electron tomography imaging have led to the fishnet SD. The fishnet SD is based on the high-resolution 3D cryo-electron tomographic images of native glomeruli that revealed an unexpected fishnet architecture, observed in mouse, fly and human. This breakthrough has provided a new and markedly different view of how we understand and analyze the SD. It provides important principles for understanding spatial self-organization, assembly, turnover, and force compensation and offers an important basis for functional investigations, including the elucidation of molecular mechanisms of clinically relevant diseases.<\/jats:p>","DOI":"10.1007\/s00424-026-03200-0","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T01:58:11Z","timestamp":1784685491000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Towards an integrative structural model of the fishnet slit diaphragm: Understanding its physiology through proteomics and structural insight"],"prefix":"10.1007","volume":"478","author":[{"given":"Achilleas S.","family":"Frangakis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra N.","family":"Birtasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Grahammer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"3200_CR1","doi-asserted-by":"publisher","first-page":"1437","DOI":"10.1056\/NEJMRA1808786\/SUPPL_FILE","volume":"384","author":"T Benzing","year":"2021","unstructured":"Benzing T, Salant D (2021) Insights into Glomerular Filtration and Albuminuria. 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