{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T11:30:01Z","timestamp":1783855801076,"version":"3.55.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2021,7,3]],"date-time":"2021-07-03T00:00:00Z","timestamp":1625270400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,7,3]],"date-time":"2021-07-03T00:00:00Z","timestamp":1625270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Planta"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1007\/s00425-021-03666-5","type":"journal-article","created":{"date-parts":[[2021,7,3]],"date-time":"2021-07-03T08:02:57Z","timestamp":1625299377000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Improved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods"],"prefix":"10.1007","volume":"254","author":[{"given":"Thuanne Pires","family":"Ribeiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela Tristan","family":"Louren\u00e7o-Tessutti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Paes","family":"de Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Vianna","family":"Morgante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro Salles","family":"Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Barrozo Jesus","family":"Lins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilanna Falc\u00e3o","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gl\u00eania Nunes","family":"Mello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Lima Pepino","family":"Macedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner Alexandre","family":"Lucena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina Mattar","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osmundo Brilhante","family":"Oliveira-Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8184-9599","authenticated-orcid":false,"given":"Maria Fatima","family":"Grossi-de-Sa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,3]]},"reference":[{"key":"3666_CR1","unstructured":"ABRAPA (2020) Estat\u00edsticas: O algod\u00e3o no mundo. Associa\u00e7\u00e3o Brasileira de Produtores de Algod\u00e3o. https:\/\/www.abrapa.com.br\/Paginas\/dados\/algodao-no-mundo. Accessed 13 Jan 2021"},{"key":"3666_CR2","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1002\/ps.5234","volume":"75","author":"W Akbar","year":"2019","unstructured":"Akbar W, Gowda A, Ahrens JE et al (2019) First transgenic trait for control of plant bugs and thrips in cotton. Pest Manag Sci 75:867\u2013877. https:\/\/doi.org\/10.1002\/ps.5234","journal-title":"Pest Manag Sci"},{"key":"3666_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s001220051441","volume":"101","author":"FJL Arag\u00e3o","year":"2000","unstructured":"Arag\u00e3o FJL, Sarokin L, Vianna GR, Rech EL (2000) Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean [Glycine max (L.) Merril] plants at a high frequency. Theor Appl Genet 101:1\u20136. https:\/\/doi.org\/10.1007\/s001220051441","journal-title":"Theor Appl Genet"},{"issue":"5","key":"3666_CR4","doi-asserted-by":"publisher","first-page":"1227","DOI":"10.1016\/j.plantsci.2004.12.024","volume":"168","author":"FJ Aragao","year":"2005","unstructured":"Aragao FJ, Vianna GR, Carvalheira SB, Rech EL (2005) Germ line genetic transformation in cotton (Gossypium hirsutum L.) by selection of transgenic meristematic cells with a herbicide molecule. Plant Sci 168(5):1227\u20131233. https:\/\/doi.org\/10.1016\/j.plantsci.2004.12.024","journal-title":"Plant Sci"},{"key":"3666_CR5","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1007\/s12033-008-9072-5","volume":"40","author":"S Asad","year":"2008","unstructured":"Asad S, Mukhtar Z, Nazir F et al (2008) Silicon carbide whisker-mediated embryogenic callus transformation of cotton (Gossypium hirsutum L.) and regeneration of salt tolerant plants. Mol Biotechnol 40:161\u2013169. https:\/\/doi.org\/10.1007\/s12033-008-9072-5","journal-title":"Mol Biotechnol"},{"key":"3666_CR6","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.plaphy.2021.05.009","volume":"165","author":"MF Basso","year":"2021","unstructured":"Basso MF, Costa JA, Ribeiro TP, Arraes FBM, Louren\u00e7o-Tessutti IT et al (2021) Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance. Plant Physiol Biochem 165:80\u201393. https:\/\/doi.org\/10.1016\/j.plaphy.2021.05.009","journal-title":"Plant Physiol Biochem"},{"key":"3666_CR7","doi-asserted-by":"publisher","first-page":"1714","DOI":"10.3316\/informit.644871058289920","volume":"7","author":"N Bibi","year":"2013","unstructured":"Bibi N, Fan K, Yuan S et al (2013) An efficient and highly reproducible approach for the selection of upland transgenic cotton produced by pollen tube pathway method. Aust J Crop Sci 7:1714\u20131722. https:\/\/doi.org\/10.3316\/informit.644871058289920","journal-title":"Aust J Crop Sci"},{"issue":"1\u20132","key":"3666_CR8","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1\u20132):248\u2013254. https:\/\/doi.org\/10.1016\/0003-2697(76)90527-3","journal-title":"Anal Biochem"},{"key":"3666_CR100","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1111\/j.1751-7915.2012.00342.x","volume":"6","author":"A Bravo","year":"2013","unstructured":"Bravo A, G\u00f3mez I, Porta H et al (2013) Evolution of Bacillus thuringiensis Cry toxins insecticidal activity. Microb Biotechnol 6:17\u201326. https:\/\/doi.org\/10.1111\/j.1751-7915.2012.00342.x","journal-title":"Microb Biotechnol"},{"key":"3666_CR9","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1093\/pcp\/pct204","volume":"55","author":"X Chen","year":"2014","unstructured":"Chen X, Wang Y, Lv B et al (2014) The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway. Plant Cell Physiol 55:604\u2013619. https:\/\/doi.org\/10.1093\/pcp\/pct204","journal-title":"Plant Cell Physiol"},{"key":"3666_CR10","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.575283","volume":"11","author":"Y Chen","year":"2020","unstructured":"Chen Y, Lange A, Vaghchhipawala Z et al (2020) Direct germline transformation of cotton meristem explants with no selection. Front Plant Sci 11:575283. https:\/\/doi.org\/10.3389\/fpls.2020.575283","journal-title":"Front Plant Sci"},{"key":"3666_CR11","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1007\/BF02668391","volume":"13","author":"CA Chlan","year":"1995","unstructured":"Chlan CA, Lin J, Cary JW, Cleveland TE (1995) A procedure for biolistic transformation and regeneration of transgenic cotton from meristematic tissue. Plant Mol Biol Rep\/ISPMB 13:31\u201337. https:\/\/doi.org\/10.1007\/BF02668391","journal-title":"Plant Mol Biol Rep\/ISPMB"},{"key":"3666_CR12","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1071\/PP9910481","volume":"18","author":"Y Cousins","year":"1991","unstructured":"Cousins Y, Lyon B, Llewellyn D (1991) Transformation of an Australian cotton cultivar: prospects for cotton improvement through genetic engineering. Funct Plant Biol 18:481. https:\/\/doi.org\/10.1071\/PP9910481","journal-title":"Funct Plant Biol"},{"key":"3666_CR13","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/978-3-642-04796-1_4","volume-title":"Cotton: biotechnological advances. Biotechnology agriculture","author":"DR Duncan","year":"2010","unstructured":"Duncan DR (2010) Cotton transformation. In: Zehr UB (ed) Cotton: biotechnological advances. Biotechnology agriculture. Springer Berlin Heidelberg, pp 65\u201377. https:\/\/doi.org\/10.1007\/978-3-642-04796-1_4"},{"issue":"1","key":"3666_CR14","doi-asserted-by":"publisher","first-page":"56","DOI":"10.3390\/genes10010056","volume":"10","author":"MA El-Esawi","year":"2019","unstructured":"El-Esawi MA, Alayafi AA (2019) Overexpression of rice Rab7 gene improves drought and heat tolerance and increases grain yield in rice (Oryza sativa L.). Genes 10(1):56. https:\/\/doi.org\/10.3390\/genes10010056","journal-title":"Genes"},{"key":"3666_CR15","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1007\/978-3-319-44570-0_10","volume-title":"Fiber plants","author":"C Emani","year":"2016","unstructured":"Emani C (2016) Transgenic cotton for agronomical useful traits. In: Ramawat KG, Ahuja MR (eds) Fiber plants. Springer Intern Publ, Cham, pp 201\u2013216"},{"key":"3666_CR16","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1007\/BF00270059","volume":"8","author":"JJ Finer","year":"1990","unstructured":"Finer JJ, McMullen MD (1990) Transformation of cotton (Gossypium hirsutum L.) via particle bombardment. Plant Cell Rep 8:586\u2013589. https:\/\/doi.org\/10.1007\/BF00270059","journal-title":"Plant Cell Rep"},{"key":"3666_CR17","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/BF00016148","volume":"10","author":"E Firoozabady","year":"1987","unstructured":"Firoozabady E, Deboer DL, Merlo DJ et al (1987) Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants. Plant Mol Biol 10:105\u2013116. https:\/\/doi.org\/10.1007\/BF00016148","journal-title":"Plant Mol Biol"},{"key":"3666_CR18","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.jretai.2008.12.001","volume":"85","author":"S Ganesan","year":"2009","unstructured":"Ganesan S, George M, Jap S et al (2009) Supply chain management and retailer performance: emerging trends, issues, and implications for research and practice. J Retail 85:84\u201394. https:\/\/doi.org\/10.1016\/j.jretai.2008.12.001","journal-title":"J Retail"},{"issue":"6","key":"3666_CR19","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1093\/jxb\/eru021","volume":"65","author":"YM Gaspar","year":"2014","unstructured":"Gaspar YM, McKenna JA, McGinness BS et al (2014) Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1. J Exp Bot 65(6):1541\u20131550. https:\/\/doi.org\/10.1093\/jxb\/eru021","journal-title":"J Exp Bot"},{"key":"3666_CR20","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1007\/s11627-019-10030-6","volume":"56","author":"P Gurusaravanan","year":"2020","unstructured":"Gurusaravanan P, Vinoth S, Jayabalan N (2020) An improved Agrobacterium-mediated transformation method for cotton (Gossypium hirsutum L. \u2018KC3\u2019) assisted by microinjection and sonication. Vitro Cell Dev Biol Plant 56:111\u2013121. https:\/\/doi.org\/10.1007\/s11627-019-10030-6","journal-title":"Vitro Cell Dev Biol Plant"},{"key":"3666_CR21","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1007\/s11262-011-0569-9","volume":"42","author":"JA Hashmi","year":"2011","unstructured":"Hashmi JA, Zafar Y, Arshad M et al (2011) Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences. Virus Genes 42:286\u2013296. https:\/\/doi.org\/10.1007\/s11262-011-0569-9","journal-title":"Virus Genes"},{"key":"3666_CR22","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1007\/978-981-15-1472-2_25","volume-title":"Cotton production and uses: agronomy, crop protection, and postharvest technologies","author":"K Hayat","year":"2020","unstructured":"Hayat K, Bardak A, Parlak D et al (2020) Biotechnology for cotton improvement. In: Ahmad S, Hasanuzzaman M (eds) Cotton production and uses: agronomy, crop protection, and postharvest technologies. Springer Singapore, Singapore, pp 509\u2013525"},{"key":"3666_CR23","unstructured":"ICAC (2020) International Cotton Advisory Committee. https:\/\/www.icac.org\/DataPortal\/DataPortal?Units=Area&Year=2019\/20%20for. Accessed 13 Jan 2021"},{"key":"3666_CR24","unstructured":"IMAmt (2015) Manual de boas pr\u00e1ticas de manejo do algodoeiro em Mato Grosso. Editores: Ima-MT e AMPA, Cuiab\u00e1\u2013Mato Grosso, Brasil (ISBN 978-85-66457-06-3)"},{"key":"3666_CR25","unstructured":"ISAAA (2018) International Service for the Acquisition of Agri-biotech Applications, GM ApprovalDatabase. https:\/\/www.isaaa.org\/resources\/publications\/biotech_crop_annual_update\/download\/biotech-crop-annual-update-cotton-2019.pdf. Accessed 13 Jan 2021"},{"key":"3666_CR26","unstructured":"ISAAA (2020) International Service for the Acquisition of Agri-biotech Applications, GM Approval Database Approval Database (http:\/\/www.isaaa.org\/gmapprovaldatabase\/advsearch\/default.asp?CropID=7&TraitTypeID=Any&DeveloperID=Any&CountryID=Any&ApprovalTypeID=Any). Accessed 13 Jan 2021"},{"key":"3666_CR27","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1007\/s11240-014-0640-z","volume":"120","author":"VN Juturu","year":"2015","unstructured":"Juturu VN, Mekala GK, Kirti PB (2015) Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.). Plant Cell Tissue Organ Cult 120:813\u2013839. https:\/\/doi.org\/10.1007\/s11240-014-0640-z","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"3666_CR28","unstructured":"Keshamma E, Rohini S, Rao KS, Madhusudhan B, Kumar MU (2008) Tissue culture-independent in planta transformation strategy: an Agrobacterium tumefaciens-mediated gene transfer method to overcome recalcitrance in cotton (Gossypium hirsutum L.). J Cotton Sci 12:264\u2013272. http:\/\/cotton.org\/journal\/2008-12\/3\/upload\/JCS12-264.pdf. Accessed 13 Jan 2021"},{"key":"3666_CR29","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1007\/s11103-004-2907-y","volume":"56","author":"S Kumar","year":"2004","unstructured":"Kumar S, Dhingra A, Daniell H (2004) Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol 56:203\u2013216. https:\/\/doi.org\/10.1007\/s11103-004-2907-y","journal-title":"Plant Mol Biol"},{"key":"3666_CR30","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s00299-003-0710-x","volume":"22","author":"S Leelavathi","year":"2004","unstructured":"Leelavathi S, Sunnichan V, Kumria R et al (2004) A simple and rapid Agrobacterium-mediated transformation protocol for cotton (Gossypium hirsutum L.): Embryogenic calli as a source to generate large numbers of transgenic plants. Plant Cell Rep 22:465\u2013470. https:\/\/doi.org\/10.1007\/s00299-003-0710-x","journal-title":"Plant Cell Rep"},{"key":"3666_CR31","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1007\/s00299-003-0751-1","volume":"22","author":"X Li","year":"2004","unstructured":"Li X, Wang XD, Zhao X, Dutt Y (2004) Improvement of cotton fiber quality by transforming the acsA and acsB genes into Gossypium hirsutum L. by means of vacuum infiltration. Plant Cell Rep 22:691\u2013697. https:\/\/doi.org\/10.1007\/s00299-003-0751-1","journal-title":"Plant Cell Rep"},{"key":"3666_CR32","doi-asserted-by":"publisher","first-page":"246","DOI":"10.3389\/fpls.2017.00246","volume":"8","author":"S Li","year":"2017","unstructured":"Li S, Cong Y, Liu Y et al (2017) Optimization of Agrobacterium-mediated transformation in soybean. Front Plant Sci 8:246. https:\/\/doi.org\/10.3389\/fpls.2017.00246","journal-title":"Front Plant Sci"},{"key":"3666_CR33","doi-asserted-by":"publisher","first-page":"1195","DOI":"10.4236\/as.2017.810087","volume":"8","author":"ME Lisei-de-Sa","year":"2017","unstructured":"Lisei-de-Sa ME, Arraes FB, Brito G et al (2017) AtDREB2A-CA influences root architecture and increases drought tolerance in transgenic cotton. Agric Sci 8:1195\u20131225. https:\/\/doi.org\/10.4236\/as.2017.810087","journal-title":"Agric Sci"},{"key":"3666_CR34","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1007\/s11240-010-9908-0","volume":"106","author":"JF Liu","year":"2011","unstructured":"Liu JF, Wang XF, Li QL et al (2011) Biolistic transformation of cotton (Gossypium hirsutum L.) with the phyA gene from Aspergillus ficum. Plant Cell Tissue Organ Cult 106:207\u2013214. https:\/\/doi.org\/10.1007\/s11240-010-9908-0","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"3666_CR35","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1038\/nbt0593-596","volume":"11","author":"DE McCabe","year":"1993","unstructured":"McCabe DE, Martinell BJ (1993) Transformation of elite cotton cultivars via particle bombardment of meristems. Nat Biotechnol 11:596\u2013598. https:\/\/doi.org\/10.1038\/nbt0593-596","journal-title":"Nat Biotechnol"},{"key":"3666_CR36","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1007\/s11627-007-9031-z","volume":"43","author":"Z-H Meng","year":"2007","unstructured":"Meng Z-H, Liang A-H, Yang W-C (2007) Effects of hygromycin on cotton cultures and its application in Agrobacterium-mediated cotton transformation. Vitro Cell Dev Biol Plant 43:111\u2013118. https:\/\/doi.org\/10.1007\/s11627-007-9031-z","journal-title":"Vitro Cell Dev Biol Plant"},{"key":"3666_CR37","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1007\/s11105-009-0102-7","volume":"27","author":"SB Nandeshwar","year":"2009","unstructured":"Nandeshwar SB, Moghe S, Chakrabarty PK et al (2009) Agrobacterium-mediated transformation of cry1Ac gene into shoot-tip meristem of diploid cotton Gossypium arboreum cv. RG8 and regeneration of transgenic plants. Plant Mol Biol Rep 27:549\u2013557. https:\/\/doi.org\/10.1007\/s11105-009-0102-7","journal-title":"Plant Mol Biol Rep"},{"key":"3666_CR39","doi-asserted-by":"publisher","first-page":"1228","DOI":"10.3389\/fpls.2020.01228","volume":"11","author":"B Paes de Melo","year":"2020","unstructured":"Paes de Melo B, Louren\u00e7o-Tessutti IT, Morgante CV et al (2020) Soybean embryonic axis transformation: combining biolistic and Agrobacterium-mediated protocols to overcome typical complications of in vitro plant regeneration. Front Plant Sci 11:1228. https:\/\/doi.org\/10.3389\/fpls.2020.01228","journal-title":"Front Plant Sci"},{"key":"3666_CR40","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1038\/nbt1090-939","volume":"8","author":"FJ Perlak","year":"1990","unstructured":"Perlak FJ, Deaton RW, Armstrong TA et al (1990) Insect resistant cotton plants. Biotechnology 8:939\u2013943. https:\/\/doi.org\/10.1038\/nbt1090-939","journal-title":"Biotechnology"},{"key":"3666_CR41","doi-asserted-by":"publisher","first-page":"519","DOI":"10.3389\/fpls.2013.00519","volume":"4","author":"A Pitzschke","year":"2013","unstructured":"Pitzschke A (2013) Agrobacterium infection and plant defense-transformation success hangs by a thread. Front Plant Sci 4:519. https:\/\/doi.org\/10.3389\/fpls.2013.00519","journal-title":"Front Plant Sci"},{"key":"3666_CR42","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1007\/s002990050770","volume":"19","author":"K Rajasekaran","year":"2000","unstructured":"Rajasekaran K, Hudspeth RL, Cary JW et al (2000) High-frequency stable transformation of cotton (Gossypium hirsutum L.) by particle bombardment of embryogenic cell suspension cultures. Plant Cell Rep 19:539\u2013545. https:\/\/doi.org\/10.1007\/s002990050770","journal-title":"Plant Cell Rep"},{"key":"3666_CR43","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1038\/nprot.2008.9","volume":"3","author":"EL Rech","year":"2008","unstructured":"Rech EL, Vianna GR, Arag\u00e3o FJL (2008) High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants. Nat Protoc 3:410\u2013418. https:\/\/doi.org\/10.1038\/nprot.2008.9","journal-title":"Nat Protoc"},{"key":"3666_CR44","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1111\/pbi.12694","volume":"15","author":"TP Ribeiro","year":"2017","unstructured":"Ribeiro TP, Arraes FBM, Louren\u00e7o-Tessutti IT et al (2017) Transgenic cotton expressing Cry10Aa toxin confers high resistance to the cotton boll weevil. Plant Biotechnol J 15:997\u20131009. https:\/\/doi.org\/10.1111\/pbi.12694","journal-title":"Plant Biotechnol J"},{"key":"3666_CR45","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.biori.2019.12.003","volume":"3","author":"TP Ribeiro","year":"2020","unstructured":"Ribeiro TP, Basso MF, Carvalho MH et al (2020) Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. Biotechnol Res Innov 3:27\u201341. https:\/\/doi.org\/10.1016\/j.biori.2019.12.003","journal-title":"Biotechnol Res Innov"},{"key":"3666_CR46","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1023\/A:1011906701925","volume":"8","author":"G Sunilkumar","year":"2001","unstructured":"Sunilkumar G, Rathore KS (2001) Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration. Mol Breed 8:37\u201352. https:\/\/doi.org\/10.1023\/A:1011906701925","journal-title":"Mol Breed"},{"key":"3666_CR47","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1038\/nbt0387-263","volume":"5","author":"P Umbeck","year":"1987","unstructured":"Umbeck P, Johnson G, Barton K, Swain W (1987) Genetically transformed cotton (Gossypium hirsutum L.) plants. Nat Biotechnol 5:263\u2013266. https:\/\/doi.org\/10.1038\/nbt0387-263","journal-title":"Nat Biotechnol"},{"key":"3666_CR48","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1080\/07352680091139286","volume":"19","author":"TA Wilkins","year":"2000","unstructured":"Wilkins TA, Rajasekaran K, Anderson DM (2000) Cotton biotechnology. Crit Rev Plant Sci 19:511\u2013550. https:\/\/doi.org\/10.1080\/07352680091139286","journal-title":"Crit Rev Plant Sci"},{"key":"3666_CR101","doi-asserted-by":"publisher","unstructured":"Yang X (2012) Analysis of the copy number of exogenous genes in transgenic cotton using real-time quantitative PCR and the 2-\u25b3\u25b3CT method. Afr J Biotechnol 11. https:\/\/doi.org\/10.5897\/AJB11.4117","DOI":"10.5897\/AJB11.4117"},{"key":"3666_CR49","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1007\/s001220051065","volume":"98","author":"C Zapata","year":"1999","unstructured":"Zapata C, Park SH, El-Zik KM, Smith RH (1999) Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex. Theor Appl Genet 98:252\u2013256. https:\/\/doi.org\/10.1007\/s001220051065","journal-title":"Theor Appl Genet"},{"key":"3666_CR50","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1023\/B:BIOP.0000047142.07987.e1","volume":"42","author":"B Zhang","year":"2001","unstructured":"Zhang B, Feng R, Wang Y, Wang Q (2001) High frequency somatic embryogenesis and plant regeneration of an elite Chinese cotton variety. Bot Bull Acad Sin 42:9\u201316. https:\/\/doi.org\/10.1023\/B:BIOP.0000047142.07987.e1","journal-title":"Bot Bull Acad Sin"},{"key":"3666_CR51","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/s0167-4781(02)00623-1","volume":"1625","author":"XD Zhang","year":"2003","unstructured":"Zhang XD, Jenkins JN, Callahan FE et al (2003) Molecular cloning, differential expression, and functional characterization of a family of class I ubiquitin-conjugating enzyme (E2) genes in cotton (Gossypium). Biochem Biophys Acta 1625:269\u2013327. https:\/\/doi.org\/10.1016\/s0167-4781(02)00623-1","journal-title":"Biochem Biophys Acta"},{"key":"3666_CR52","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1007\/s10529-006-9078-7","volume":"28","author":"F-Y Zhao","year":"2006","unstructured":"Zhao F-Y, Li Y-F, Xu P (2006) Agrobacterium-mediated transformation of cotton (Gossypium hirsutum L. cv. Zhongmian 35) using glyphosate as a selectable marker. Biotechnol Lett 28:1199\u20131207. https:\/\/doi.org\/10.1007\/s10529-006-9078-7","journal-title":"Biotechnol Lett"},{"key":"3666_CR53","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1079\/IVP2006777","volume":"42","author":"S-W Zhu","year":"2006","unstructured":"Zhu S-W, Gao P, Sun J-S et al (2006) Genetic transformation of green-colored cotton. Vitro Cell Dev Biol Plant 42:439\u2013444. https:\/\/doi.org\/10.1079\/IVP2006777","journal-title":"Vitro Cell Dev Biol Plant"}],"container-title":["Planta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00425-021-03666-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00425-021-03666-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00425-021-03666-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,8]],"date-time":"2021-08-08T16:05:07Z","timestamp":1628438707000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00425-021-03666-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,3]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["3666"],"URL":"https:\/\/doi.org\/10.1007\/s00425-021-03666-5","relation":{},"ISSN":["0032-0935","1432-2048"],"issn-type":[{"value":"0032-0935","type":"print"},{"value":"1432-2048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,3]]},"assertion":[{"value":"13 April 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 June 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 July 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be interpreted as a potential conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}}],"article-number":"20"}}