{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:46:46Z","timestamp":1792028806006,"version":"4.4.0"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Planta"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s00425-026-05194-6","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:39:44Z","timestamp":1792028384000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering cotton with ASAL and MYB genes confer dual benefits of pest resistance and fiber improvement"],"prefix":"10.1007","volume":"264","author":[{"given":"Ayesha","family":"Imran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saira","family":"Azam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha","family":"Latif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naila","family":"Shahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneela","family":"Yasmeen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahir Rehman","family":"Sammiullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sania","family":"Naeem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Ali","family":"Baig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul Qayyum","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Ali","family":"Shahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"5194_CR1","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.indcrop.2018.12.070","volume":"130","author":"A Abdelraheem","year":"2019","unstructured":"Abdelraheem A, Esmaeili N, O\u2019Connell M, Zhang J (2019) Progress and perspective on drought and salt stress tolerance in cotton. Ind Crops Prod 130:118\u2013129","journal-title":"Ind Crops Prod"},{"key":"5194_CR2","doi-asserted-by":"publisher","DOI":"10.1093\/aobpla\/plw008","volume":"8","author":"MA Abid","year":"2016","unstructured":"Abid MA, Malik W, Yasmeen A, Qayyum A, Zhang R, Liang C, Guo S, Ashraf J (2016) Mode of inheritance for biochemical traits in genetically engineered cotton under water stress. AoB Plants 8:plw008","journal-title":"AoB Plants"},{"key":"5194_CR3","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.476251","volume":"11","author":"M Ahmed","year":"2020","unstructured":"Ahmed M, Iqbal A, Latif A, Din SU, Sarwar MB, Wang X, Rao AQ, Husnain T, Ali Shahid A (2020) Overexpression of a sucrose synthase gene indirectly improves cotton fiber quality through sucrose cleavage. Front Plant Sci 11:476251","journal-title":"Front Plant Sci"},{"key":"5194_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2022.111576","volume":"328","author":"S Akhtar","year":"2023","unstructured":"Akhtar S, Shahid AA, Shakoor S, Ahmed M, Iftikhar S, Usmaan M, Sadaqat S, Latif A, Iqbal A, Rao AQ (2023) Tissue specific expression of bacterial cellulose synthase (Bcs) genes improves cotton fiber length and strength. Plant Sci 328:111576","journal-title":"Plant Sci"},{"key":"5194_CR5","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1002\/9781119385523.ch12","volume-title":"Cotton production","author":"MA Ali","year":"2019","unstructured":"Ali MA, Farooq J, Batool A, Zahoor A, Azeem F, Mahmood A, Jabran K (2019) Cotton production in Pakistan. In: Jabran K, Chauhan BD (eds) Cotton production. Wiley, pp 249\u2013276"},{"key":"5194_CR6","first-page":"206","volume":"7","author":"KS Bajwa","year":"2013","unstructured":"Bajwa KS, Shahid AA, Rao AQ, Kiani MS, Ashraf MA, Dahab AA, Bakhsh A, Latif A, Khan MAU, Puspito AN (2013) Expression of Calotropis procera expansin gene CpEXPA3 enhances cotton fibre strength. Aust J Crop Sci 7:206\u2013212","journal-title":"Aust J Crop Sci"},{"key":"5194_CR7","first-page":"333","volume":"1143","author":"A Bakhsh","year":"2015","unstructured":"Bakhsh A, Zia M, Hussain T, Tekeli F, Gokce A (2015) Members of Alliaceae; better source of plant lectins to combat resistance against sucking pests of crops. VII InterSymp Edible Alliaceae 1143:333\u2013340","journal-title":"VII InterSymp Edible Alliaceae"},{"key":"5194_CR8","doi-asserted-by":"publisher","first-page":"1426","DOI":"10.20546\/ijcmas.2018.709.171","volume":"7","author":"Y Bharathi","year":"2018","unstructured":"Bharathi Y, Reddy V, Rao K, Pasalu I (2018) Effect of mannose specific lectins ASAL and GNA on the feeding behavior of BPH (Nilaparvata lugens stal.). Int J Curr Microbiol App Sci 7:1426\u20131436","journal-title":"Int J Curr Microbiol App Sci"},{"key":"5194_CR9","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1093\/pcp\/pcv019","volume":"56","author":"T Chen","year":"2015","unstructured":"Chen T, Li W, Hu X, Guo J, Liu A, Zhang B (2015) A cotton MYB transcription factor, GbMYB5, is positively involved in plant adaptive response to drought stress. Plant Cell Physiol 56:917\u2013929","journal-title":"Plant Cell Physiol"},{"key":"5194_CR10","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1007\/s00299-021-02669-6","volume":"40","author":"S Din","year":"2021","unstructured":"Din S, Azam S, Rao AQ, Shad M, Ahmed M, Gul A, Latif A, Ali MA, Husnain T, Shahid AA (2021) Development of broad-spectrum and sustainable resistance in cotton against major insects through the combination of Bt and plant lectin genes. Plant Cell Rep 40:707\u2013721","journal-title":"Plant Cell Rep"},{"key":"5194_CR11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-65249-w","volume":"10","author":"A Gul","year":"2020","unstructured":"Gul A, Hussain G, Iqbal A, Rao AQ, Din SU, Yasmeen A, Shahid N, Ahad A, Latif A, Azam S (2020) Constitutive expression of asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci. Sci Rep 10:8958","journal-title":"Sci Rep"},{"key":"5194_CR12","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1111\/tpj.13273","volume":"88","author":"H Hu","year":"2016","unstructured":"Hu H, He X, Tu L, Zhu L, Zhu S, Ge Z, Zhang X (2016) GhJAZ2 negatively regulates cotton fiber initiation by interacting with the R2R3-MYB transcription factor GhMYB25-like. Plant J 88:921\u2013935","journal-title":"Plant J"},{"key":"5194_CR13","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1093\/pcp\/pcy238","volume":"60","author":"J Huang","year":"2019","unstructured":"Huang J, Guo Y, Sun Q, Zeng W, Li J, Li X, Xu W (2019) Genome-wide identification of R2R3-MYB transcription factors regulating secondary cell wall thickening in cotton fiber development. Plant Cell Physiol 60:687\u2013701","journal-title":"Plant Cell Physiol"},{"key":"5194_CR14","first-page":"360","volume-title":"Molecular physiology of abiotic stresses in plant productivity","author":"P Kumar","year":"2018","unstructured":"Kumar P, Dwivedi P (2018) Plant lectins, agricultural advancements and mammalian toxicity. In: Hemantaranjan A (ed) Molecular physiology of abiotic stresses in plant productivity. Scientific Publishers, Jodhpur, pp 360\u2013378"},{"key":"5194_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13104-015-1397-0","volume":"8","author":"A Latif","year":"2015","unstructured":"Latif A, Rao AQ, Khan MAU, Shahid N, Bajwa KS, Ashraf MA, Abbas MA, Azam M, Shahid AA, Nasir IA (2015) Herbicide-resistant cotton (Gossypium hirsutum) plants: an alternative way of manual weed removal. BMC Res Notes 8:1\u20138","journal-title":"BMC Res Notes"},{"issue":"2","key":"5194_CR35","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1111\/pbr.12247","volume":"134","author":"B Li","year":"2015","unstructured":"Li B, Yang Y, Hu WR, Li XD, Cao JQ, Fan L (2015) Over\u2010expression of Gh UGP 1 in upland cotton improves fibre quality and reduces fibre sugar content. Plant Breeding 134(2):197\u2013202","journal-title":"Plant Breeding"},{"issue":"12","key":"5194_CR16","doi-asserted-by":"publisher","first-page":"1282","DOI":"10.1016\/j.tplants.2024.07.013","volume":"29","author":"L Li","year":"2024","unstructured":"Li L, Zhang Z, Zhang B (2024) iJAZ: the next breakthrough for engineering pest-resistance in plants? Trends Plant Sci 29(12):1282\u20131284","journal-title":"Trends Plant Sci"},{"key":"5194_CR17","doi-asserted-by":"publisher","first-page":"2014","DOI":"10.3390\/molecules20022014","volume":"20","author":"MLR Macedo","year":"2015","unstructured":"Macedo MLR, Oliveira CF, Oliveira CT (2015) Insecticidal activity of plant lectins and potential application in crop protection. Molecules 20:2014\u20132033","journal-title":"Molecules"},{"key":"5194_CR18","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1007\/s12033-011-9476-5","volume":"52","author":"RC Muoki","year":"2012","unstructured":"Muoki RC, Paul A, Kumari A, Singh K, Kumar S (2012) An improved protocol for the isolation of RNA from roots of tea (Camellia sinensis (L.) O. Kuntze). Mol Biotechnol 52:82\u201388","journal-title":"Mol Biotechnol"},{"key":"5194_CR19","doi-asserted-by":"publisher","first-page":"299","DOI":"10.4172\/2157-7471.1000263","volume":"6","author":"K Nath","year":"2015","unstructured":"Nath K, Solanky KU, Mahatma MK, Madhubala SR, Rakesh M (2015) Role of total soluble sugar, phenols and defense related enzymes in relation to banana fruit rot by Lasiodiplodia theobromae [(Path) Griff and Maubl] during ripening. J. Plant Pathol Microbiol 6:299. https:\/\/doi.org\/10.4172\/2157-7471.1000263","journal-title":"J. Plant Pathol Microbiol"},{"key":"5194_CR20","doi-asserted-by":"publisher","first-page":"943","DOI":"10.3389\/fpls.2015.00943","volume":"6","author":"AN Puspito","year":"2015","unstructured":"Puspito AN, Rao AQ, Hafeez MN, Iqbal MS, Bajwa KS, Ali Q, Rashid B, Abbas MA, Latif A, Shahid AA (2015) Transformation and evaluation of Cry1Ac+Cry2A and GTGene in Gossypium hirsutum L. Front Plant Sci 6:943","journal-title":"Front Plant Sci"},{"issue":"11","key":"5194_CR21","doi-asserted-by":"publisher","first-page":"3135","DOI":"10.1111\/pbi.14436","volume":"22","author":"G Qi","year":"2024","unstructured":"Qi G, Si Z, Xuan L, Han Z, Hu Y, Fang L, Dai F, Zhang T (2024) Unravelling the genetic basis and regulation networks related to fibre quality improvement using chromosome segment substitution lines in cotton. Plant Biotechnol J 22(11):3135\u20133150","journal-title":"Plant Biotechnol J"},{"key":"5194_CR22","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1631\/jzus.B1000168","volume":"12","author":"AQ Rao","year":"2011","unstructured":"Rao AQ, Irfan M, Saleem Z, Nasir IA, Riazuddin S, Husnain T (2011) Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum). J Zhejiang Univ Sci B 12:326\u2013334","journal-title":"J Zhejiang Univ Sci B"},{"issue":"19","key":"5194_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s42397-021-00094-4","volume":"4","author":"A Sahar","year":"2021","unstructured":"Sahar A, Zafar MM, Razzaq A et al (2021) Genetic variability for yield and fiber related traits in genetically modified cotton. J Cotton Res 4(19):1\u201310. https:\/\/doi.org\/10.1186\/s42397-021-00094-4","journal-title":"J Cotton Res"},{"key":"5194_CR24","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/S0168-9452(01)00547-7","volume":"162","author":"V Satyavathi","year":"2002","unstructured":"Satyavathi V, Prasad V, Lakshmi BG, Sita GL (2002) High efficiency transformation protocol for three Indian cotton varieties via Agrobacterium tumefaciens. Plant Sci 162:215\u2013223","journal-title":"Plant Sci"},{"key":"5194_CR25","doi-asserted-by":"publisher","first-page":"5315","DOI":"10.1007\/s11033-021-06972-z","volume":"49","author":"M Shad","year":"2022","unstructured":"Shad M, Yasmeen A, Azam S, Bakhsh A, Latif A, Shahid N, Sadaqat S, Rao AQ, Shahid AA (2022) Enhancing the resilience of transgenic cotton for insect resistance. Mol Biol Rep 49:5315\u20135323. https:\/\/doi.org\/10.1007\/s11033-021-06972-z","journal-title":"Mol Biol Rep"},{"key":"5194_CR26","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1111\/plb.13299","volume":"23","author":"X Shangguan","year":"2021","unstructured":"Shangguan X, Yang Q, Wu X, Cao J (2021) Function analysis of a cotton R2R3 MYB transcription factor GhMYB3 in regulating plant trichome development. Plant Biol 23:1118\u20131127","journal-title":"Plant Biol"},{"key":"5194_CR27","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1111\/ppl.12317","volume":"154","author":"X Sun","year":"2015","unstructured":"Sun X, Gong SY, Nie XY, Li Y, Li W, Huang GQ, Li XB (2015) A R2R3-MYB transcription factor that is specifically expressed in cotton (Gossypium hirsutum) fibers affects secondary cell wall biosynthesis and deposition in transgenic Arabidopsis. Physiol Plant 154:420\u2013432","journal-title":"Physiol Plant"},{"key":"5194_CR28","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.bbaexp.2003.08.009","volume":"1630","author":"J Suo","year":"2003","unstructured":"Suo J, Liang X, Pu L, Zhang Y, Xue Y (2003) Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (Gossypium hirsutum L.). Biochim Biophys Acta (BBA) Gene Struct Expr 1630:25\u201334","journal-title":"Biochim Biophys Acta (BBA) Gene Struct Expr"},{"issue":"3","key":"5194_CR29","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/S0003-2697(69)80009-6","volume":"32","author":"DM Updegraff","year":"1969","unstructured":"Updegraff DM (1969) Semimicro determination of cellulose inbiological materials. Anal Biochem 32(3):420\u2013424","journal-title":"Anal Biochem"},{"key":"5194_CR30","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1111\/nph.14844","volume":"217","author":"H Wu","year":"2018","unstructured":"Wu H, Tian Y, Wan Q, Fang L, Guan X, Chen J, Hu Y, Ye W, Zhang H, Guo W (2018) Genetics and evolution of MIXTA genes regulating cotton lint fiber development. New Phytol 217:883\u2013895","journal-title":"New Phytol"},{"key":"5194_CR31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0230519","volume":"15","author":"A Yaqoob","year":"2020","unstructured":"Yaqoob A, Ali Shahid A, Salisu IB, Shakoor S, Usmaan M, Shad M, Rao AQ (2020) Comparative analysis of constitutive and fiber-specific promoters under the expression pattern of expansin gene in transgenic cotton. PLoS ONE 15:e0230519","journal-title":"PLoS ONE"},{"issue":"21","key":"5194_CR32","first-page":"3771","volume":"12","author":"Z Zhai","year":"2023","unstructured":"Zhai Z, Zhang K, Fang Y, Yang Y, Cao X, Liu L, Tian Y (2023) Systematically and comprehensively understanding the regulation of cotton fiber initiation: a review. Plants (Basel) 12(21):3771","journal-title":"Plants (Basel)"},{"key":"5194_CR33","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1038\/nbt.3207","volume":"33","author":"T Zhang","year":"2015","unstructured":"Zhang T, Hu Y, Jiang W, Fang L, Guan X, Chen J, Zhang J, Saski CA, Scheffler BE, Stelly DM (2015) Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotechnol 33:531\u2013537","journal-title":"Nat Biotechnol"},{"issue":"6","key":"5194_CR34","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1016\/j.cj.2024.11.001","volume":"12","author":"X Zhao","year":"2024","unstructured":"Zhao X, He Y, Liu Y, Wang Z, Zhao J (2024) JAZ proteins: key regulators of plant growth and stress response. Crop J 12(6):1505\u20131516","journal-title":"Crop J"}],"container-title":["Planta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00425-026-05194-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00425-026-05194-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00425-026-05194-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:39:45Z","timestamp":1792028385000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00425-026-05194-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,14]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["5194"],"URL":"https:\/\/doi.org\/10.1007\/s00425-026-05194-6","relation":{},"ISSN":["0032-0935","1432-2048"],"issn-type":[{"value":"0032-0935","type":"print"},{"value":"1432-2048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,14]]},"assertion":[{"value":"7 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 October 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"There is no conflict of interest among authors.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"168"}}