{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T14:56:33Z","timestamp":1772722593890,"version":"3.50.1"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2016,8,27]],"date-time":"2016-08-27T00:00:00Z","timestamp":1472256000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["KL595\/2-3"],"award-info":[{"award-number":["KL595\/2-3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004937","name":"Bundesministerium f\u00fcr Forschung und Technologie","doi-asserted-by":"publisher","award":["13EZ0817"],"award-info":[{"award-number":["13EZ0817"]}],"id":[{"id":"10.13039\/501100004937","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virchows Arch"],"published-print":{"date-parts":[[2016,11]]},"DOI":"10.1007\/s00428-016-2008-8","type":"journal-article","created":{"date-parts":[[2016,8,26]],"date-time":"2016-08-26T23:02:21Z","timestamp":1472252541000},"page":"553-562","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A new monoclonal antibody (Cox mAB 31A2) detects VP1 protein of coxsackievirus B3 with high sensitivity and specificity"],"prefix":"10.1007","volume":"469","author":[{"given":"Nicole","family":"Ettischer-Schmid","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Normann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Sauter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Kraft","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Kalbacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Kandolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertram","family":"Flehmig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Klingel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,8,27]]},"reference":[{"key":"2008_CR1","doi-asserted-by":"publisher","first-page":"1222","DOI":"10.1086\/315818","volume":"182","author":"L Andreoletti","year":"2000","unstructured":"Andreoletti L, Bourlet T, Moukassa D, Rey L, Hot D, Li Y, Lambert V, Gosselin B, Mosnier JF, Stankowiak C, Wattre P (2000) Enteroviruses can persist with or without active viral replication in cardiac tissue of patients with end-stage ischemic or dilated cardiomyopathy. J Infect Dis 182:1222\u20131227. doi: 10.1086\/315818","journal-title":"J Infect Dis"},{"key":"2008_CR2","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1002\/(SICI)1096-9071(199706)52:2<206::AID-JMV15>3.0.CO;2-I","volume":"52","author":"L Andreoletti","year":"1997","unstructured":"Andreoletti L, Hober D, Becquart P, Belaich S, Copin MC, Lambert V, Wattre P (1997) Experimental CVB3-induced chronic myocarditis in two murine strains: evidence of interrelationships between virus replication and myocardial damage in persistent cardiac infection. J Med Virol 52:206\u2013214","journal-title":"J Med Virol"},{"key":"2008_CR3","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S0046-8177(87)80363-5","volume":"18","author":"HT Aretz","year":"1987","unstructured":"Aretz HT (1987) Myocarditis: the Dallas criteria. Hum Pathol 18:619\u2013624","journal-title":"Hum Pathol"},{"key":"2008_CR4","doi-asserted-by":"publisher","unstructured":"Blauwet LA, Cooper LT Myocarditis Prog Cardiovasc Dis 52:274\u2013288. doi: 10.1016\/j.pcad.2009.11.006","DOI":"10.1016\/j.pcad.2009.11.006"},{"key":"2008_CR5","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1002\/elps.1150080203","volume":"8","author":"H Blum","year":"1987","unstructured":"Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins. RNA and DNA in polyacrylamide gels Electrophoresis 8:93\u201399. doi: 10.1002\/elps.1150080203","journal-title":"RNA and DNA in polyacrylamide gels Electrophoresis"},{"key":"2008_CR6","doi-asserted-by":"publisher","first-page":"197","DOI":"10.2144\/000113382","volume":"48","author":"J Bordeaux","year":"2010","unstructured":"Bordeaux J, Welsh A, Agarwal S, Killiam E, Baquero M, Hanna J, Anagnostou V, Rimm D (2010) Antibody validation BioTechniques 48:197\u2013209. doi: 10.2144\/000113382","journal-title":"Antibody validation BioTechniques"},{"key":"2008_CR7","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1080\/08916930701619235","volume":"41","author":"AL Caforio","year":"2008","unstructured":"Caforio AL, Tona F, Bottaro S, Vinci A, Dequal G, Daliento L, Thiene G, Iliceto S (2008) Clinical implications of anti-heart autoantibodies in myocarditis and dilated cardiomyopathy. Autoimmunity 41:35\u201345. doi: 10.1080\/08916930701619235","journal-title":"Autoimmunity"},{"key":"2008_CR8","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1073\/pnas.89.4.1320","volume":"89","author":"MW Cunningham","year":"1992","unstructured":"Cunningham MW, Antone SM, Gulizia JM, McManus BM, Fischetti VA, Gauntt CJ (1992) Cytotoxic and viral neutralizing antibodies crossreact with streptococcal M protein, enteroviruses, and human cardiac myosin. Proc Natl Acad Sci U S A 89:1320\u20131324","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2008_CR9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0022-1759(80)90146-5","volume":"35","author":"SF StGroth de","year":"1980","unstructured":"de StGroth SF, Scheidegger D (1980) Production of monoclonal antibodies: strategy and tactics. J Immunol Methods 35:1\u201321","journal-title":"J Immunol Methods"},{"key":"2008_CR10","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1002\/path.4166","volume":"229","author":"SF Hansson","year":"2013","unstructured":"Hansson SF, Korsgren S, Ponten F, Korsgren O (2013) Enteroviruses and the pathogenesis of type 1 diabetes revisited: cross-reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins. J Pathol 229:719\u2013728. doi: 10.1002\/path.4166","journal-title":"J Pathol"},{"key":"2008_CR11","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1073\/pnas.89.1.314","volume":"89","author":"K Klingel","year":"1992","unstructured":"Klingel K, Hohenadl C, Canu A, Albrecht M, Seemann M, Mall G, Kandolf R (1992) Ongoing enterovirus-induced myocarditis is associated with persistent heart muscle infection: quantitative analysis of virus replication, tissue damage, and inflammation. Proc Natl Acad Sci U S A 89:314\u2013318","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2008_CR12","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/s00430-003-0190-1","volume":"193","author":"K Klingel","year":"2004","unstructured":"Klingel K, Sauter M, Bock CT, Szalay G, Schnorr JJ, Kandolf R (2004) Molecular pathology of inflammatory cardiomyopathy. Med Microbiol Immunol 193:101\u2013107. doi: 10.1007\/s00430-003-0190-1","journal-title":"Med Microbiol Immunol"},{"key":"2008_CR13","doi-asserted-by":"crossref","first-page":"8888","DOI":"10.1128\/JVI.70.12.8888-8895.1996","volume":"70","author":"K Klingel","year":"1996","unstructured":"Klingel K, Stephan S, Sauter M, Zell R, McManus BM, Bultmann B, Kandolf R (1996) Pathogenesis of murine enterovirus myocarditis: virus dissemination and immune cell targets. J Virol 70:8888\u20138895","journal-title":"J Virol"},{"key":"2008_CR14","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680\u2013685","journal-title":"Nature"},{"key":"2008_CR15","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1161\/01.CIR.101.3.231","volume":"101","author":"Y Li","year":"2000","unstructured":"Li Y, Bourlet T, Andreoletti L, Mosnier JF, Peng T, Yang Y, Archard LC, Pozzetto B, Zhang H (2000) Enteroviral capsid protein VP1 is present in myocardial tissues from some patients with myocarditis or dilated cardiomyopathy. Circulation 101:231\u2013234","journal-title":"Circulation"},{"issue":"Suppl D","key":"2008_CR16","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1093\/eurheartj\/12.suppl_D.18","volume":"12","author":"BM McManus","year":"1991","unstructured":"McManus BM, Chow LH, Radio SJ, Tracy SM, Beck MA, Chapman NM, Klingel K, Kandolf R (1991) Progress and challenges in the pathological diagnosis of myocarditis. Eur Heart J 12(Suppl D):18\u201321","journal-title":"Eur Heart J"},{"key":"2008_CR17","first-page":"655","volume-title":"Fields virology","author":"J Melnick","year":"1996","unstructured":"Melnick J (1996) Enteroviruses: poliovirus, coxsackieviruses, echoviruses and newer enteroviruses. In: Fields BN, Knipe D, Howley P (eds) Fields virology. Lippincott-Raven, New York, pp. 655\u2013712"},{"key":"2008_CR18","doi-asserted-by":"publisher","unstructured":"Mercalli A, Lampasona V, Klingel K, Albarello L, Lombardoni C, Ekstrom J, Sordi V, Bolla A, Mariani A, Bzhalava D, Dillner J, Roivainen M, Bosi E, Piemonti L No evidence of enteroviruses in the intestine of patients with type 1 diabetes. Diabetologia 55:2479\u20132488. doi: 10.1007\/s00125-012-2591-4","DOI":"10.1007\/s00125-012-2591-4"},{"key":"2008_CR19","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1007\/s10741-012-9357-4","volume":"18","author":"S Pankuweit","year":"2013","unstructured":"Pankuweit S, Klingel K (2013) Viral myocarditis: from experimental models to molecular diagnosis in patients. Heart Fail Rev 18:683\u2013702. doi: 10.1007\/s10741-012-9357-4 Review","journal-title":"Heart Fail Rev"},{"key":"2008_CR20","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.yexmp.2015.12.007","volume":"100","author":"DJ Pisapia","year":"2016","unstructured":"Pisapia DJ, Lavi E (2016) VZV, temporal arteritis, and clinical practice: false positive immunohistochemical detection due to antibody cross-reactivity. Exp Mol Pathol 100:114\u2013115. doi: 10.1016\/j.yexmp.2015.12.007","journal-title":"Exp Mol Pathol"},{"key":"2008_CR21","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1007\/s00125-013-3094-7","volume":"57","author":"SJ Richardson","year":"2014","unstructured":"Richardson SJ, Leete P, Dhayal S, Russell MA, Oikarinen M, Laiho JE, Svedin E, Lind K, Rosenling T, Chapman N, Bone AJ, Foulis AK, Frisk G, Flodstrom-Tullberg M, Hober D, Hyoty H, Morgan NG (2014) Evaluation of the fidelity of immunolabelling obtained with clone 5D8\/1, a monoclonal antibody directed against the enteroviral capsid protein, VP1, in human pancreas. Diabetologia 57:392\u2013401. doi: 10.1007\/s00125-013-3094-7","journal-title":"Diabetologia"},{"key":"2008_CR22","doi-asserted-by":"publisher","first-page":"1143","DOI":"10.1007\/s00125-009-1276-0","volume":"52","author":"SJ Richardson","year":"2009","unstructured":"Richardson SJ, Willcox A, Bone AJ, Foulis AK, Morgan NG (2009) The prevalence of enteroviral capsid protein vp1 immunostaining in pancreatic islets in human type 1 diabetes. Diabetologia 52:1143\u20131151. doi: 10.1007\/s00125-009-1276-0","journal-title":"Diabetologia"},{"key":"2008_CR23","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1007\/s00125-009-1332-9","volume":"52","author":"M Roivainen","year":"2009","unstructured":"Roivainen M, Klingel K (2009) Role of enteroviruses in the pathogenesis of type 1 diabetes. Diabetologia 52:995\u2013996. doi: 10.1007\/s00125-009-1332-9","journal-title":"Diabetologia"},{"key":"2008_CR24","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1093\/clinids\/20.4.971","volume":"20","author":"HA Rotbart","year":"1995","unstructured":"Rotbart HA (1995) Enteroviral infections of the central nervous system. Clin Infect Dis 20:971\u2013981","journal-title":"Clin Infect Dis"},{"key":"2008_CR25","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1128\/CDLI.1.3.336-341.1994","volume":"1","author":"A Samuelson","year":"1994","unstructured":"Samuelson A, Forsgren M, Johansson B, Wahren B, Sallberg M (1994) Molecular basis for serological cross-reactivity between enteroviruses. Clin Diagn Lab Immunol 1:336\u2013341","journal-title":"Clin Diagn Lab Immunol"},{"key":"2008_CR26","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1128\/CDLI.2.3.385-386.1995","volume":"2","author":"A Samuelson","year":"1995","unstructured":"Samuelson A, Forsgren M, Sallberg M (1995) Characterization of the recognition site and diagnostic potential of an enterovirus group-reactive monoclonal antibody. Clin Diagn Lab Immunol 2:385\u2013386","journal-title":"Clin Diagn Lab Immunol"},{"key":"2008_CR27","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1016\/S0025-6196(11)60670-8","volume":"84","author":"JC Schultz","year":"2009","unstructured":"Schultz JC, Hilliard AA, Cooper LT Jr, Rihal CS (2009) Diagnosis and treatment of viral myocarditis. Mayo Clin Proc 84:1001\u20131009. doi: 10.1016\/S0025-6196(11)60670-8","journal-title":"Mayo Clin Proc"},{"key":"2008_CR28","doi-asserted-by":"publisher","first-page":"4108","DOI":"10.1096\/fj.13-230193","volume":"27","author":"K Steinke","year":"2013","unstructured":"Steinke K, Sachse F, Ettischer N, Strutz-Seebohm N, Henrion U, Rohrbeck M, Klosowski R, Wolters D, Brunner S, Franz WM, Pott L, Munoz C, Kandolf R, Schulze-Bahr E, Lang F, Klingel K, Seebohm G (2013) Coxsackievirus B3 modulates cardiac ion channels. FASEB J 27:4108\u20134121. doi: 10.1096\/fj.13-230193","journal-title":"FASEB J"},{"key":"2008_CR29","first-page":"52","volume":"33","author":"ON Ursu","year":"2014","unstructured":"Ursu ON, Sauter M, Ettischer N, Kandolf R, Klingel K (2014) Heme oxygenase-1 mediates oxidative stress and apoptosis in coxsackievirus B3-induced myocarditis cellular. Physiology and Biochemistry 33:52\u201366","journal-title":"Physiology and Biochemistry"},{"key":"2008_CR30","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1089\/dna.1.1988.7.307","volume":"7","author":"S Werner","year":"1988","unstructured":"Werner S, Klump WM, Schonke H, Hofschneider PH, Kandolf R (1988) Expression of coxsackievirus B3 capsid proteins in Escherichia coli and generation of virus-specific antisera. DNA 7:307\u2013316","journal-title":"DNA"},{"key":"2008_CR31","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1159\/000150012","volume":"28","author":"GE Yousef","year":"1987","unstructured":"Yousef GE, Brown IN, Mowbray JF (1987) Derivation and biochemical characterization of an enterovirus group-specific monoclonal antibody. Intervirology 28:163\u2013170","journal-title":"Intervirology"},{"key":"2008_CR32","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1159\/000150017","volume":"28","author":"GE Yousef","year":"1987","unstructured":"Yousef GE, Mann GF, Brown IN, Mowbray JF (1987) Clinical and research application of an enterovirus group-reactive monoclonal antibody. Intervirology 28:199\u2013205","journal-title":"Intervirology"}],"container-title":["Virchows Archiv"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00428-016-2008-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00428-016-2008-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00428-016-2008-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T15:40:06Z","timestamp":1601048406000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00428-016-2008-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,27]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["2008"],"URL":"https:\/\/doi.org\/10.1007\/s00428-016-2008-8","relation":{},"ISSN":["0945-6317","1432-2307"],"issn-type":[{"value":"0945-6317","type":"print"},{"value":"1432-2307","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,27]]}}}