{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:58:38Z","timestamp":1778918318699,"version":"3.51.4"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T00:00:00Z","timestamp":1562025600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T00:00:00Z","timestamp":1562025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virchows Arch"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1007\/s00428-019-02616-7","type":"journal-article","created":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T07:01:12Z","timestamp":1562050872000},"page":"313-323","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study"],"prefix":"10.1007","volume":"475","author":[{"given":"Fabio","family":"Aimi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria-Giuseppina","family":"Procopio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Teresa","family":"Alvarez Flores","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Philippe","family":"Brouland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Piazzon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sa\u0161ka","family":"Brajkovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego Gabriel","family":"Dupouy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Gijs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence","family":"de Leval","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,7,2]]},"reference":[{"issue":"2","key":"2616_CR1","doi-asserted-by":"publisher","first-page":"908","DOI":"10.3390\/cancers7020815","volume":"7","author":"A McGuire","year":"2015","unstructured":"McGuire A, Brown J, Malone C, McLaughlin R, Kerin M (2015) Effects of age on the detection and management of breast cancer. Cancers (Basel) 7(2):908\u2013929","journal-title":"Cancers (Basel)"},{"issue":"5","key":"2616_CR2","doi-asserted-by":"publisher","first-page":"E359","DOI":"10.1002\/ijc.29210","volume":"136","author":"J Ferlay","year":"2015","unstructured":"Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F (2015) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136(5):E359\u2013E386","journal-title":"Int J Cancer"},{"key":"2616_CR3","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/513158","volume":"2014","author":"BK Banin Hirata","year":"2014","unstructured":"Banin Hirata BK, Oda JM, Losi Guembarovski R, Ariza CB, de Oliveira CE, Watanabe MA (2014) Molecular markers for breast cancer: prediction on tumor behavior. Dis Markers 2014:513158","journal-title":"Dis Markers"},{"issue":"3","key":"2616_CR4","doi-asserted-by":"publisher","first-page":"382","DOI":"10.5306\/wjco.v5.i3.382","volume":"5","author":"DC Zaha","year":"2014","unstructured":"Zaha DC (2014) Significance of immunohistochemistry in breast cancer. World J Clin Oncol 5(3):382\u2013392","journal-title":"World J Clin Oncol"},{"issue":"2","key":"2616_CR5","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1016\/j.cll.2018.02.006","volume":"38","author":"S Bandyopadhyay","year":"2018","unstructured":"Bandyopadhyay S, Bluth MH, Ali-Fehmi R (2018) Breast carcinoma: updates in molecular profiling 2018. Clin Lab Med 38(2):401\u2013420","journal-title":"Clin Lab Med"},{"issue":"14","key":"2616_CR6","doi-asserted-by":"publisher","first-page":"5363","DOI":"10.1073\/pnas.1211273110","volume":"110","author":"AT Ciftlik","year":"2013","unstructured":"Ciftlik AT, Lehr H-A, Gijs MAM (2013) Microfluidic processor allows rapid HER2 immunohistochemistry of breast carcinomas and significantly reduces ambiguous (2+) read-outs. Proc Natl Acad Sci 110(14):5363\u20135368","journal-title":"Proc Natl Acad Sci"},{"key":"2616_CR7","doi-asserted-by":"crossref","unstructured":"Dupouy DG et al (2016) Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer. Sci Rep 6","DOI":"10.1038\/srep20277"},{"issue":"8","key":"2616_CR8","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1038\/labinvest.2017.49","volume":"97","author":"S Brajkovic","year":"2017","unstructured":"Brajkovic S, Dupouy DG, De Leval L, Gijs MAM (2017) Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections. Lab Investig 97(8):983\u2013991","journal-title":"Lab Investig"},{"issue":"16","key":"2616_CR9","doi-asserted-by":"publisher","first-page":"2784","DOI":"10.1200\/JCO.2009.25.6529","volume":"28","author":"MEH Hammond","year":"2010","unstructured":"Hammond MEH et al (2010) American Society of Clinical Oncology\/College of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. J Clin Oncol 28(16):2784\u20132795","journal-title":"J Clin Oncol"},{"issue":"31","key":"2616_CR10","doi-asserted-by":"publisher","first-page":"3997","DOI":"10.1200\/JCO.2013.50.9984","volume":"31","author":"AC Wolff","year":"2013","unstructured":"Wolff AC, Hammond ME, Hicks DG, Dowsett M, McShane L, Allison KH, Allred DC, Bartlett JM, Bilous M, Fitzgibbons P, Hanna W, Jenkins RB, Mangu PB, Paik S, Perez EA, Press MF, Spears PA, Vance GH, Viale G, Hayes DF, American Society of Clinical Oncology, College of American Pathologists (2013) Recommendations for human epidermal growth factor receptor 2 testing in breast cancer: American Society of Clinical Oncology\/College of American Pathologists clinical practice guideline update. TL - 31. J Clin Oncol 31(31):3997\u20134013","journal-title":"J Clin Oncol"},{"key":"2616_CR11","unstructured":"Coates AS et al (2015) Tailoring therapies-improving the management of early breast cancer: St Gallen International Expert Consensus on the Primary Therapy of Early Breast Cancer 2015. Ann Oncol"},{"issue":"1","key":"2616_CR12","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1038\/labinvest.2016.121","volume":"97","author":"HT Nguyen","year":"2017","unstructured":"Nguyen HT, Trouillon R, Matsuoka S, Fiche M, de Leval L, Bisig B, Gijs MA (2017) Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment in breast cancer. Lab Investig 97(1):93\u2013103","journal-title":"Lab Investig"},{"issue":"10","key":"2616_CR13","doi-asserted-by":"publisher","first-page":"1402","DOI":"10.5858\/arpa.2016-0497-OA","volume":"141","author":"ML Troxell","year":"2017","unstructured":"Troxell ML, Long T, Hornick JL, Ambaye AB, Jensen KC (2017) Comparison of estrogen and progesterone receptor antibody reagents using proficiency testing data. Arch Pathol Lab Med 141(10):1402\u20131412","journal-title":"Arch Pathol Lab Med"},{"key":"2616_CR14","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.anndiagpath.2018.02.007","volume":"35","author":"BC Calhoun","year":"2018","unstructured":"Calhoun BC, Mosteller B, Warren D, Smith M, Jordi Rowe J, Lanigan CP, Mrazeck KC, Walker E, Newell AH, Jones R (2018) Analytical and clinical performance of progesterone receptor antibodies in breast cancer. Ann Diagn Pathol 35:21\u201326","journal-title":"Ann Diagn Pathol"},{"issue":"22","key":"2616_CR15","doi-asserted-by":"publisher","first-page":"7003","DOI":"10.1158\/1078-0432.CCR-09-0449","volume":"15","author":"P Roepman","year":"2009","unstructured":"Roepman P, Horlings HM, Krijgsman O, Kok M, Bueno-de-Mesquita JM, Bender R, Linn SC, Glas AM, van de Vijver M (2009) Microarray-based determination of estrogen receptor, progesterone receptor, and HER2 receptor status in breast cancer. Clin Cancer Res 15(22):7003\u20137011","journal-title":"Clin Cancer Res"},{"key":"2616_CR16","doi-asserted-by":"crossref","unstructured":"Prat A et al (2013) Prognostic significance of progesterone receptor-positive tumor cells within immunohistochemically defined luminal a breast cancer. J Clin Oncol","DOI":"10.1200\/JCO.2012.43.4134"},{"key":"2616_CR17","doi-asserted-by":"publisher","first-page":"2206","DOI":"10.1093\/annonc\/mdt303","volume":"24","author":"A Goldhirsch","year":"2013","unstructured":"Goldhirsch A, Winer EP, Coates AS, Gelber RD, Piccart-Gebhart M, Th\u00fcrlimann B, Senn HJ, Albain KS, Andr\u00e9 F, Bergh J, Bonnefoi H, Bretel-Morales D, Burstein H, Cardoso F, Castiglione-Gertsch M, Coates AS, Colleoni M, Costa A, Curigliano G, Davidson NE, di Leo A, Ejlertsen B, Forbes JF, Gelber RD, Gnant M, Goldhirsch A, Goodwin P, Goss PE, Harris JR, Hayes DF, Hudis CA, Ingle JN, Jassem J, Jiang Z, Karlsson P, Loibl S, Morrow M, Namer M, Kent Osborne C, Partridge AH, Penault-Llorca F, Perou CM, Piccart-Gebhart MJ, Pritchard KI, Rutgers EJT, Sedlmayer F, Semiglazov V, Shao ZM, Smith I, Th\u00fcrlimann B, Toi M, Tutt A, Untch M, Viale G, Watanabe T, Wilcken N, Winer EP, Wood WC (2013) Personalizing the treatment of women with early breast cancer: highlights of the St Gallen International Expert Consensus on the Primary Therapy of Early Breast Cancer 2013. Ann Oncol 24:2206\u20132223","journal-title":"Ann Oncol"}],"container-title":["Virchows Archiv"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00428-019-02616-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00428-019-02616-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00428-019-02616-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:21:18Z","timestamp":1593562878000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00428-019-02616-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,2]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["2616"],"URL":"https:\/\/doi.org\/10.1007\/s00428-019-02616-7","relation":{},"ISSN":["0945-6317","1432-2307"],"issn-type":[{"value":"0945-6317","type":"print"},{"value":"1432-2307","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,2]]},"assertion":[{"value":"28 March 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 June 2019","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 June 2019","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2019","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"At the submission of the paper, DGD and SB are employed by Lunaphore Technologies SA, which is commercializing a microfluidic-based staining device. DGD and MAMG have equity interest in Lunaphore Technologies SA. The other authors declare no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"A total of 50 FFPE breast cancer tissue specimens were retrieved from the Pathology Institute of the University Hospital of Lausanne (CHUV) between January 2016 and April 2017 with the ethical committee approval (protocol number 511-12).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}