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The most common subtype of invasive breast carcinoma, invasive carcinoma of no special type (IBC-NST), is known for its stromal loss of CD34+ fibroblasts while acquiring alpha smooth muscle actin-positive (\u03b1-SMA+) myofibroblasts, i.e., cancer-associated fibroblasts (CAF), whereas invasive lobular carcinoma (ILC) displays partial preservation of CD34+ fibroblasts. The aim of this study was to evaluate the prognostic relevance of stromal CD34+ fibroblasts and \u03b1-SMA+ myofibroblasts in an extended collection of ILC. A total of 133 cases of ILC, primarily resected between 1996 and 2004 at University Hospital Marburg, were examined semiquantitatively for stromal content of CD34+ fibroblasts and \u03b1-SMA+ myofibroblasts. Partial preservation of CD34+ fibroblasts in the tumor stroma of ILC was confirmed. Absence of CD34+ fibroblasts in the tumor stroma significantly correlated with the presence of \u03b1-SMA+ myofibroblasts (<jats:italic>p<\/jats:italic>\u2009=\u20090.010), positive lymph node status (<jats:italic>p<\/jats:italic>\u2009=\u20090.004), and pN stage (<jats:italic>p<\/jats:italic>\u2009=\u20090.006). Stromal loss of CD34+ fibroblasts was significantly associated with lower overall and disease-free survival rates (<jats:italic>p<\/jats:italic>\u2009=\u20090.012 and 0.013, respectively). Multivariate analysis adjusted for pT and pN stage revealed stromal loss of CD34+ fibroblasts as independent prognostic parameter (<jats:italic>p<\/jats:italic>\u2009=\u20090.05). To our knowledge, this is the first report defining prognostically relevant stromal subtypes of ILC with long-term follow-up. Future research targeting the potential diagnostic and therapeutic implications of CD34+ fibroblasts and CAF in breast cancer, especially ILC, is a promising field of interest.<\/jats:p>","DOI":"10.1007\/s00428-020-02835-3","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T04:42:59Z","timestamp":1589949779000},"page":"717-724","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Prognostic relevance of the loss of stromal CD34 positive fibroblasts in invasive lobular carcinoma of the breast"],"prefix":"10.1007","volume":"477","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6812-3563","authenticated-orcid":false,"given":"Christina C.","family":"Westhoff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Jank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian O.","family":"Jacke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute-Susann","family":"Albert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Schokufe","family":"Ebrahimsade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Barth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Moll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,20]]},"reference":[{"key":"2835_CR1","unstructured":"Robert-Koch-Institut Krebs in Deutschland | 2015\/2016"},{"key":"2835_CR2","unstructured":"Lakhani SR, Rakha E, Simpson PT (2012) Invasive lobular carcinoma. 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