{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T21:36:11Z","timestamp":1794346571599,"version":"4.9.0"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["PRIN 2017ZXT5WR"],"award-info":[{"award-number":["PRIN 2017ZXT5WR"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017727","name":"Fondazione Beretta","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017727","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["23747"],"award-info":[{"award-number":["23747"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virchows Arch"],"published-print":{"date-parts":[[2024,7]]},"DOI":"10.1007\/s00428-023-03682-8","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T19:01:40Z","timestamp":1698346900000},"page":"43-51","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Immunoglobulin light chain transcript detection by ultrasensitive RNA in situ hybridization for B-cell lymphoma diagnosis"],"prefix":"10.1007","volume":"485","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2483-2955","authenticated-orcid":false,"given":"Luisa","family":"Lorenzi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Lonardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Bonezzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Zini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Bugatti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Valzelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Melotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Facchetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iacopo","family":"Ghini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Villanacci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piera","family":"Balzarini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Pizzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Giustini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Galvagni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Chiarini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo Paolo","family":"Dei Tos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Vermi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Casola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Facchetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,26]]},"reference":[{"key":"3682_CR1","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1097\/PAI.0000000000000787","volume":"28","author":"M Landry","year":"2020","unstructured":"Landry M, Bienz MN, Sawan B, Temmar R, Beauregard P, Chaunt F, Lavigne J, Knecht H (2020) Bone marrow immunohistochemistry and flow cytometry in the diagnosis of malignant hematologic diseases with emphasis on lymphomas: a comparative retrospective study. Appl Immunohistochem Mol Morphol 28:508\u2013512. https:\/\/doi.org\/10.1097\/PAI.0000000000000787","journal-title":"Appl Immunohistochem Mol Morphol"},{"key":"3682_CR2","doi-asserted-by":"publisher","first-page":"2159","DOI":"10.1038\/leu.2012.246","volume":"26","author":"AW Langerak","year":"2012","unstructured":"Langerak AW, Groenen PJ, Bruggemann M, Beldjord K, Bellan C, Bonello L, Boone E, Carter GI, Catherwood M, Davi F, Delfau-Larue MH, Diss T, Evans PA, Gameiro P, Garcia Sanz R, Gonzalez D, Grand D, Hakansson A, Hummel M, Liu H, Lombardia L, Macintyre EA, Milner BJ, Montes-Moreno S, Schuuring E, Spaargaren M, Hodges E, van Dongen JJ (2012) EuroClonality\/BIOMED-2 guidelines for interpretation and reporting of Ig\/TCR clonality testing in suspected lymphoproliferations. Leukemia 26:2159\u20132171. https:\/\/doi.org\/10.1038\/leu.2012.246","journal-title":"Leukemia"},{"key":"3682_CR3","doi-asserted-by":"publisher","first-page":"2227","DOI":"10.1038\/s41375-019-0508-7","volume":"33","author":"B Scheijen","year":"2019","unstructured":"Scheijen B, Meijers RWJ, Rijntjes J, van der Klift MY, Mobs M, Steinhilber J, Reigl T, van den Brand M, Kotrova M, Ritter JM, Catherwood MA, Stamatopoulos K, Bruggemann M, Davi F, Darzentas N, Pott C, Fend F, Hummel M, Langerak AW, Groenen P (2019) EuroClonality NGSWG Next-generation sequencing of immunoglobulin gene rearrangements for clonality assessment: a technical feasibility study by EuroClonality-NGS. Leukemia 33:2227\u20132240. https:\/\/doi.org\/10.1038\/s41375-019-0508-7","journal-title":"Leukemia"},{"key":"3682_CR4","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1097\/00019606-200303000-00002","volume":"12","author":"RC Beck","year":"2003","unstructured":"Beck RC, Tubbs RR, Hussein M, Pettay J, Hsi ED (2003) Automated colorimetric in situ hybridization (CISH) detection of immunoglobulin (Ig) light chain mRNA expression in plasma cell (PC) dyscrasias and non-Hodgkin lymphoma. Diagn Mol Pathol 12:14\u201320. https:\/\/doi.org\/10.1097\/00019606-200303000-00002","journal-title":"Diagn Mol Pathol"},{"key":"3682_CR5","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jmoldx.2011.08.002","volume":"14","author":"F Wang","year":"2012","unstructured":"Wang F, Flanagan J, Su N, Wang LC, Bui S, Nielson A, Wu X, Vo HT, Ma XJ, Luo Y (2012) RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn 14:22\u201329. https:\/\/doi.org\/10.1016\/j.jmoldx.2011.08.002","journal-title":"J Mol Diagn"},{"key":"3682_CR6","unstructured":"Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, Stein H, Thiele J (2017)\u00a0\u00a0(Eds): WHO classification of tumours of haematopoietic and lymphoid tissues\u00a0(Revised 4th edition).\u00a0IARC:\u00a0Lyon"},{"key":"3682_CR7","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1309\/AJCPJTWK07FSABRJ","volume":"140","author":"RR Tubbs","year":"2013","unstructured":"Tubbs RR, Wang H, Wang Z, Minca EC, Portier BP, Gruver AM, Lanigan C, Luo Y, Cook JR, Ma XJ (2013) Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low-abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders. Am J Clin Pathol 140:736\u2013746. https:\/\/doi.org\/10.1309\/AJCPJTWK07FSABRJ","journal-title":"Am J Clin Pathol"},{"key":"3682_CR8","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1038\/modpathol.2017.142","volume":"31","author":"L Guo","year":"2018","unstructured":"Guo L, Wang Z, Anderson CM, Doolittle E, Kernag S, Cotta CV, Ondrejka SL, Ma XJ, Cook JR (2018) Ultrasensitive automated RNA in situ hybridization for kappa and lambda light chain mRNA detects B-cell clonality in tissue biopsies with performance comparable or superior to flow cytometry. Mod Pathol 31:385\u2013394. https:\/\/doi.org\/10.1038\/modpathol.2017.142","journal-title":"Mod Pathol"},{"key":"3682_CR9","doi-asserted-by":"publisher","first-page":"1078","DOI":"10.1097\/PAS.0000000000001881","volume":"46","author":"H Kaseb","year":"2022","unstructured":"Kaseb H, Wang Z, Cook JR (2022) Ultrasensitive RNA in situ hybridization for kappa and lambda light chains assists in the differential diagnosis of nodular lymphocyte-predominant Hodgkin lymphoma. Am J Surg Pathol 46:1078\u20131083. https:\/\/doi.org\/10.1097\/PAS.0000000000001881","journal-title":"Am J Surg Pathol"},{"key":"3682_CR10","doi-asserted-by":"publisher","first-page":"3301","DOI":"10.3109\/10428194.2015.1034702","volume":"56","author":"A Ohmoto","year":"2015","unstructured":"Ohmoto A, Maeshima AM, Taniguchi H, Tanioka K, Makita S, Kitahara H, Fukuhara S, Munakata W, Suzuki T, Maruyama D, Kobayashi Y, Tobinai K (2015) Histopathological analysis of B-cell non-Hodgkin lymphomas without light chain restriction by using flow cytometry. Leuk Lymphoma 56:3301\u20133305. https:\/\/doi.org\/10.3109\/10428194.2015.1034702","journal-title":"Leuk Lymphoma"},{"key":"3682_CR11","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1309\/AJCP3C2QZZBPTMLB","volume":"136","author":"P Horna","year":"2011","unstructured":"Horna P, Olteanu H, Kroft SH, Harrington AM (2011) Flow cytometric analysis of surface light chain expression patterns in B-cell lymphomas using monoclonal and polyclonal antibodies. Am J Clin Pathol 136:954\u2013959. https:\/\/doi.org\/10.1309\/AJCP3C2QZZBPTMLB","journal-title":"Am J Clin Pathol"},{"key":"3682_CR12","first-page":"110","volume":"5","author":"DD Grier","year":"2012","unstructured":"Grier DD, Al-Quran SZ, Cardona DM, Li Y, Braylan RC (2012) Flow cytometric analysis of immunoglobulin heavy chain expression in B-cell lymphoma and reactive lymphoid hyperplasia Int J. Clin Exp Pathol 5:110\u2013118","journal-title":"Clin Exp Pathol"},{"key":"3682_CR13","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/s0002-9440(10)63815-1","volume":"162","author":"SA Pileri","year":"2003","unstructured":"Pileri SA, Gaidano G, Zinzani PL, Falini B, Gaulard P, Zucca E, Pieri F, Berra E, Sabattini E, Ascani S, Piccioli M, Johnson PW, Giardini R, Pescarmona E, Novero D, Piccaluga PP, Marafioti T, Alonso MA, Cavalli F (2003) Primary mediastinal B-cell lymphoma: high frequency of BCL-6 mutations and consistent expression of the transcription factors OCT-2, BOB.1, and PU.1 in the absence of immunoglobulins. Am J Pathol 162:243\u2013253. https:\/\/doi.org\/10.1016\/s0002-9440(10)63815-1","journal-title":"Am J Pathol"},{"key":"3682_CR14","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1309\/AJCP3Q1DHWKCQRWY","volume":"144","author":"OK Weinberg","year":"2015","unstructured":"Weinberg OK, Rodig SJ, Pozdnyakova O, Ren L, Arber DA, Ohgami RS (2015) Surface light chain expression in primary mediastinal large B-cell lymphomas by multiparameter flow cytometry. Am J Clin Pathol 144:635\u2013641. https:\/\/doi.org\/10.1309\/AJCP3Q1DHWKCQRWY","journal-title":"Am J Clin Pathol"},{"key":"3682_CR15","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1002\/cyto.b.21544","volume":"94","author":"S Cherian","year":"2018","unstructured":"Cherian S, Fromm JR (2018) Evaluation of primary mediastinal large B cell lymphoma by flow cytometry. Cytometry B Clin Cytom 94:459\u2013467. https:\/\/doi.org\/10.1002\/cyto.b.21544","journal-title":"Cytometry B Clin Cytom"},{"key":"3682_CR16","doi-asserted-by":"publisher","first-page":"1411","DOI":"10.1097\/01.pas.0000180856.74572.73","volume":"29","author":"A Traverse-Glehen","year":"2005","unstructured":"Traverse-Glehen A, Pittaluga S, Gaulard P, Sorbara L, Alonso MA, Raffeld M, Jaffe ES (2005) Mediastinal gray zone lymphoma: the missing link between classic Hodgkin\u2019s lymphoma and mediastinal large B-cell lymphoma. Am J Surg Pathol 29:1411\u20131421. https:\/\/doi.org\/10.1097\/01.pas.0000180856.74572.73","journal-title":"Am J Surg Pathol"}],"updated-by":[{"DOI":"10.1007\/s00428-023-03705-4","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000}}],"container-title":["Virchows Archiv"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00428-023-03682-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00428-023-03682-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00428-023-03682-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T07:04:39Z","timestamp":1721372679000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00428-023-03682-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,26]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["3682"],"URL":"https:\/\/doi.org\/10.1007\/s00428-023-03682-8","relation":{"correction":[{"id-type":"doi","id":"10.1007\/s00428-023-03705-4","asserted-by":"object"}]},"ISSN":["0945-6317","1432-2307"],"issn-type":[{"value":"0945-6317","type":"print"},{"value":"1432-2307","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,26]]},"assertion":[{"value":"22 May 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 October 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 October 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 October 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 November 2023","order":5,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Correction","order":6,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"A Correction to this paper has been published:","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"https:\/\/doi.org\/10.1007\/s00428-023-03705-4","URL":"https:\/\/doi.org\/10.1007\/s00428-023-03705-4","order":8,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Experiments included in this study on human tissue were approved by the Local Ethical Committee (study FF-BCR2, code NP 3719).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical issues"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}