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The differential diagnosis of NC is broad, including obstructive and non-obstructive causes. In addition, histologic features of certain disorders may be non-specific in the early course of the disease. To evaluate liver biopsies using a practical histopathologic approach for NC and to define a simple scoring system for biliary atresia (BA) for routine clinical practice. From June 2006 to December 2021, liver biopsy specimens from infants with persistent NC were examined by two independent pathologists. The cases diagnosed as BA were correlated with clinical, radiologic, and laboratory data to calculate the final score. Four hundred and fifty-nine cases were enrolled in the study. They had a mean age of 63.94 \u00b1 20.62 days and were followed for a median time of 58 (1\u2013191) months. They included 162 (35.3%) cases of BA. On multivariate analysis, portal edema, ductular proliferation, cholangiolitis, and bile duct\/ductular plugs were the histopathologic predictors of BA. A liver biopsy did perform well with a 95.1% sensitivity, 91.6% specificity, 86% PPV, and 97.1% NPV. At a cutoff of 5 of the scoring system, diagnosis of BA could be done with a sensitivity of 95.1% and a specificity of 100%. We have shown detailed histopathologic features of BA with more depth to infants aged \u2264 6 weeks. We have developed a simple scoring system using a combination of liver biopsy with non-invasive methods to increase the diagnostic accuracy of BA.<\/jats:p>","DOI":"10.1007\/s00428-023-03704-5","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T02:01:46Z","timestamp":1701050506000},"page":"93-102","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pathologic approach to Neonatal cholestasis with a simple scoring system for biliary atresia"],"prefix":"10.1007","volume":"484","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7556-7173","authenticated-orcid":false,"given":"Khadiga M.","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaled R.","family":"Zalata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarik","family":"Barakat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherine M.","family":"Elzeiny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"3704_CR1","first-page":"283","volume":"26","author":"D Alagille","year":"1969","unstructured":"Alagille D, Gautier M, Habib E, Dommergues J (1969) Pre-and postoperative hepatic biopsy data in prolonged cholestasis in infants. 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