{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T14:04:03Z","timestamp":1763993043043,"version":"3.45.0"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virchows Arch"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We report the first well-documented case of in situ follicular B-cell neoplasm (ISFN) arising in splenosis in a patient with a remote history of splenectomy. A 60-year-old man undergoing evaluation for hydrocele was incidentally found to have multiple mesenteric masses on imaging. His past surgical history was notable for splenectomy 40\u00a0years earlier following a motor vehicle accident. Biopsy of the largest mass revealed fragments of splenic parenchyma containing two small follicles infiltrated by atypical lymphocytes. Follicular architecture was preserved without expansion or disruption. Immunohistochemistry demonstrated that the neoplastic cells were positive for CD20, PAX5, CD10, BCL2, and BCL6 and negative for CD3 and CD5. Flow cytometry performed on a concurrent specimen identified a clonal CD10-positive aberrant B-cell population. Fluorescence in situ hybridization (FISH) revealed a chromosomal translocation, t(14;18). These findings supported a diagnosis of ISFN.<\/jats:p>","DOI":"10.1007\/s00428-025-04322-z","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T01:34:03Z","timestamp":1763948043000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Splenosis involved by in situ follicular B-cell neoplasm"],"prefix":"10.1007","author":[{"given":"Mahreen","family":"Hussain","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kseniia","family":"Malkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandr","family":"Romanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faisal","family":"Rawas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gulrukh K.","family":"Botiralieva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalya","family":"Pak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaroslav N.","family":"Chernov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9983-9735","authenticated-orcid":false,"given":"Kirill A.","family":"Lyapichev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"4322_CR1","unstructured":"Xerri, L., In situ follicular B-cell neoplasm. 5 ed. The 5th edition of the World Health Organization classification of haematolymphoid tumours: lymphoid neoplasms. 2022, Lyon: IARC Press. 1720\u20131748."},{"issue":"9","key":"4322_CR2","doi-asserted-by":"publisher","first-page":"3376","DOI":"10.1182\/blood.V99.9.3376","volume":"99","author":"P Cong","year":"2002","unstructured":"Cong P et al (2002) In situ localization of follicular lymphoma: description and analysis by laser capture microdissection. Blood 99(9):3376\u20133382","journal-title":"Blood"},{"issue":"5","key":"4322_CR3","doi-asserted-by":"publisher","first-page":"802","DOI":"10.3324\/haematol.2013.085548","volume":"99","author":"E Mamessier","year":"2014","unstructured":"Mamessier E et al (2014) Nature and importance of follicular lymphoma precursors. Haematologica 99(5):802\u2013810","journal-title":"Haematologica"},{"issue":"11","key":"4322_CR4","doi-asserted-by":"publisher","first-page":"2976","DOI":"10.1182\/blood-2011-05-355255","volume":"118","author":"AG Jegalian","year":"2011","unstructured":"Jegalian AG et al (2011) Follicular lymphoma in situ: clinical implications and comparisons with partial involvement by follicular lymphoma. Blood 118(11):2976\u20132984","journal-title":"Blood"},{"key":"4322_CR5","doi-asserted-by":"crossref","unstructured":"Arora V, S.M., In-situ follicular neoplasia: a case series. Journal on Oncology. 3(1): p. 1089.","DOI":"10.52768\/2692-563X\/1089"},{"issue":"6","key":"4322_CR6","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1111\/his.14535","volume":"79","author":"GS Tamber","year":"2021","unstructured":"Tamber GS et al (2021) In-situ follicular neoplasia: a clinicopathological spectrum. Histopathology 79(6):1072\u20131086","journal-title":"Histopathology"},{"issue":"10","key":"4322_CR7","doi-asserted-by":"publisher","first-page":"2673","DOI":"10.3324\/haematol.2020.254854","volume":"106","author":"A Vogelsberg","year":"2021","unstructured":"Vogelsberg A et al (2021) Genetic evolution of in situ follicular neoplasia to aggressive B-cell lymphoma of germinal center subtype. Haematologica 106(10):2673\u20132681","journal-title":"Haematologica"},{"issue":"1","key":"4322_CR8","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1111\/j.1365-2559.2011.03897.x","volume":"59","author":"T Henopp","year":"2011","unstructured":"Henopp T et al (2011) Prevalence of follicular lymphoma in situ in consecutively analysed reactive lymph nodes. Histopathology 59(1):139\u2013142","journal-title":"Histopathology"},{"issue":"7","key":"4322_CR9","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1097\/PAS.0000000000000628","volume":"40","author":"G Bermudez","year":"2016","unstructured":"Bermudez G et al (2016) Incidental and isolated follicular lymphoma in situ and mantle cell lymphoma in situ lack clinical significance. Am J Surg Pathol 40(7):943\u2013949","journal-title":"Am J Surg Pathol"},{"issue":"3","key":"4322_CR10","doi-asserted-by":"publisher","first-page":"482","DOI":"10.3109\/10428194.2013.807926","volume":"55","author":"A Carbone","year":"2014","unstructured":"Carbone A, Gloghini A (2014) Emerging issues after the recognition of in situ follicular lymphoma. Leuk Lymphoma 55(3):482\u2013490","journal-title":"Leuk Lymphoma"},{"issue":"5","key":"4322_CR11","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1111\/j.1365-2559.2010.03529.x","volume":"56","author":"S Montes-Moreno","year":"2010","unstructured":"Montes-Moreno S et al (2010) Intrafollicular neoplasia\/in situ follicular lymphoma: review of a series of 13 cases. Histopathology 56(5):658\u2013662","journal-title":"Histopathology"},{"issue":"5","key":"4322_CR12","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1309\/AJCPF9V8XRDYWTIR","volume":"131","author":"MT Howard","year":"2009","unstructured":"Howard MT et al (2009) Follicular lymphoma of the spleen: multiparameter analysis of 16 cases. Am J Clin Pathol 131(5):656\u2013662","journal-title":"Am J Clin Pathol"},{"issue":"15","key":"4322_CR13","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1111\/1759-7714.14878","volume":"14","author":"T Uchida","year":"2023","unstructured":"Uchida T et al (2023) In situ follicular neoplasm discovered as an enlarging pulmonary nodule complicated by pulmonary aspergillosis. Thorac Cancer 14(15):1401\u20131403","journal-title":"Thorac Cancer"},{"issue":"3","key":"4322_CR14","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1097\/DAD.0000000000002579","volume":"46","author":"P Bavi","year":"2024","unstructured":"Bavi P et al (2024) In situ follicular B-cell neoplasm presenting as miliary facial papules: a unique clinical presentation and spontaneous regression. 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The collection and evaluation of all protected patient health information were performed in a HIPAA-compliant manner.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Patient consent was not required as de-identified data was used.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Written informed consent to publication was obtained from the patient.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"No materials were reproduced from other sources.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Permission to reproduce material from other sources"}}]}}