{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:09:34Z","timestamp":1787612974499,"version":"build-2736575974"},"reference-count":68,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T00:00:00Z","timestamp":1625184000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T00:00:00Z","timestamp":1625184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PID2019-103969RB-C33"],"award-info":[{"award-number":["PID2019-103969RB-C33"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PID2019-103969RB-C31"],"award-info":[{"award-number":["PID2019-103969RB-C31"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["BES-2017-079708"],"award-info":[{"award-number":["BES-2017-079708"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["AGL2016-74857-C3-3-R"],"award-info":[{"award-number":["AGL2016-74857-C3-3-R"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003339","name":"Consejo Superior de Investigaciones Cientificas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Brain Struct Funct"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Serotonin (5-HT) is one of the principal neurotransmitters in the nervous system of vertebrates. It is initially synthesized by hydroxylation of tryptophan (Trp) by means of tryptophan hydroxylase or TPH which is the rate-limiting enzyme in the production of 5-HT. In most vertebrates, there are two isoforms of TPH present, TPH1 and TPH2, which exhibit different catalytic or substrate specificity as well as different expression domains. Studies carried out in mammals show that only <jats:italic>tph2<\/jats:italic> is expressed in the brain whereas <jats:italic>tph1<\/jats:italic>-mRNA is primarily localized in the enterochromaffin cells and pineal gland. A large number of neurons are also considered to be serotonergic or \u201cpseudo-serotonergic\u201d as they accumulate and release 5-HT yet do not produce it as no amine-synthetic enzymes are expressed, yet a combination of 5-HT transporters is observed. Therefore, <jats:italic>tph<\/jats:italic> expression is considered to be the only specific marker of 5-HT-producing neurons that can discriminate true 5-HT from pseudo-serotonergic neurons. This work examined in situ hybridization to study the mRNA distribution of one paralogue for <jats:italic>tph1<\/jats:italic> and <jats:italic>tph2<\/jats:italic> in the central nervous system of rainbow trout. Results show a segregated expression for both paralogues that predominantly match previous immunocytochemical studies. This study thus adds valuable information to the scarce analyses focusing on the central distribution of the expression of serotonergic markers, particularly <jats:italic>tphs<\/jats:italic>, in the vertebrate brain thus characterizing the true serotonergic brain territories.<\/jats:p>","DOI":"10.1007\/s00429-021-02322-8","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T08:02:59Z","timestamp":1625212979000},"page":"2265-2278","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Distribution of two isoforms of tryptophan hydroxylase in the brain of rainbow trout (Oncorhynchus mykiss). An in situ hybridization study"],"prefix":"10.1007","volume":"226","author":[{"given":"Mauro","family":"Chivite","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Leal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas M.","family":"M\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1405-5750","authenticated-orcid":false,"given":"Jose Miguel","family":"Cerd\u00e1-Reverter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,2]]},"reference":[{"key":"2322_CR1","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1002\/(SICI)1096-9861(19990510)407:3<333::AID-CNE3>3.0.CO;2-R","volume":"407","author":"F Adrio","year":"1999","unstructured":"Adrio F, Anad\u00f3n R, Rodr\u00edguez-Moldes I (1999) Distribution of serotonin (5HT)-immunoreactive structures in the central nervous system of two chondrostean species (Acipenser baeri and Huso huso). J Comp Neurol 407:333\u2013348. https:\/\/doi.org\/10.1002\/(SICI)1096-9861(19990510)407:3%3c333::AID-CNE3%3e3.0.CO;2-R","journal-title":"J Comp Neurol"},{"key":"2322_CR2","doi-asserted-by":"publisher","first-page":"1711","DOI":"10.1126\/science.282.5394.1711","volume":"282","author":"A Amores","year":"1998","unstructured":"Amores A, Force A, Yan YL et al (1998) Zebrafish hox clusters and vertebrate genome evolution. Science 282:1711\u20131714. https:\/\/doi.org\/10.1126\/science.282.5394.1711","journal-title":"Science"},{"key":"2322_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2019.107423","volume":"205","author":"M Bader","year":"2020","unstructured":"Bader M (2020) Inhibition of serotonin synthesis: a novel therapeutic paradigm. Pharmacol Ther 205:107423. https:\/\/doi.org\/10.1016\/j.pharmthera.2019.107423","journal-title":"Pharmacol Ther"},{"key":"2322_CR4","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.jchemneu.2008.06.001","volume":"36","author":"A Barreiro-Iglesias","year":"2008","unstructured":"Barreiro-Iglesias A, Villar-Cervi\u00f1o V, Anad\u00f3n R, Rodicio MC (2008) Development and organization of the descending serotonergic brainstem-spinal projections in the sea lamprey. J Chem Neuroanat 36:77\u201384. https:\/\/doi.org\/10.1016\/j.jchemneu.2008.06.001","journal-title":"J Chem Neuroanat"},{"key":"2322_CR5","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/S0925-4773(03)00119-9","volume":"119","author":"G Bellipanni","year":"2002","unstructured":"Bellipanni G, Rink E, Bally-Cuif L (2002) Cloning of two tryptophan hydroxylase genes expressed in the diencephalon of the developing zebrafish brain. Mech Dev 119:215\u2013220. https:\/\/doi.org\/10.1016\/S0925-4773(03)00119-9","journal-title":"Mech Dev"},{"key":"2322_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1051\/rnd:19820101","volume":"22","author":"R Billard","year":"1982","unstructured":"Billard R, Peter RE (1982) A stereotaxic atlas and technique for nuclei of the diencephalon of rainbow trout (Salmo gairdneri). Reprod Nutr Dev 22:1\u201325. https:\/\/doi.org\/10.1051\/rnd:19820101","journal-title":"Reprod Nutr Dev"},{"key":"2322_CR7","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1111\/jfb.12446","volume":"85","author":"A Birba","year":"2014","unstructured":"Birba A, Ramallo MR, Morandini L et al (2014) The pineal complex in the cichlid Cichlasoma dimerus: effect of different photoperiods on its cell morphology. J Fish Biol 85:605\u2013620. https:\/\/doi.org\/10.1111\/jfb.12446","journal-title":"J Fish Biol"},{"key":"2322_CR8","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1016\/j.nlm.2009.01.009","volume":"91","author":"RE Carpenter","year":"2009","unstructured":"Carpenter RE, Summers CH (2009) Learning strategies during fear conditioning. Neurobiol Learn Mem 91:415\u2013423. https:\/\/doi.org\/10.1016\/j.nlm.2009.01.009","journal-title":"Neurobiol Learn Mem"},{"key":"2322_CR9","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1016\/j.ygcen.2008.01.003","volume":"156","author":"RM Ceinos","year":"2008","unstructured":"Ceinos RM, Polakof S, Illamola AR et al (2008) Food deprivation and refeeding effects on pineal indoles metabolism and melatonin synthesis in the rainbow trout Oncorhynchus mykiss. Gen Comp Endocrinol 156:410\u2013417. https:\/\/doi.org\/10.1016\/j.ygcen.2008.01.003","journal-title":"Gen Comp Endocrinol"},{"key":"2322_CR10","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1002\/1097-4687(200103)247:3<229::AID-JMOR1014>3.0.CO;2-K","volume":"247","author":"JMZ Cerd\u00e1-Reverter","year":"2001","unstructured":"Cerd\u00e1-Reverter JMZ, S., Mu\u00f1oz-Cuento JA. (2001) Cytoarchitectonic study of the brain of a perciform species, the Sea Bass (Dicentrarchus labrax). II. The Diencephalon. J Morphol 247:229\u2013251. https:\/\/doi.org\/10.1002\/1097-4687(200103)247:3%3c229::AID-JMOR1014%3e3.0.CO;2-K","journal-title":"J Morphol"},{"key":"2322_CR11","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1007\/s00429-012-0412-7","volume":"218","author":"ME Cornide-Petronio","year":"2013","unstructured":"Cornide-Petronio ME, Anad\u00f3n R, Rodicio MC, Barreiro-Iglesias A (2013) The sea lamprey tryptophan hydroxylase: new insight into the evolution of the serotonergic system of vertebrates. Brain Struct Funct 218:587\u2013593. https:\/\/doi.org\/10.1007\/s00429-012-0412-7","journal-title":"Brain Struct Funct"},{"key":"2322_CR12","doi-asserted-by":"publisher","first-page":"4322","DOI":"10.1073\/pnas.84.12.4322","volume":"84","author":"RJ D\u2019Amato","year":"1987","unstructured":"D\u2019Amato RJ, Blue ME, Largent BL, Lynch DR, Ledbetter DJ, Molliver ME, Snyder SH (1987) Ontogeny of the serotonergic projection to rat neocortex: transient expression of a dense innervation to primary sensory areas. Proc Natl Acad Sci USA 84:4322\u20134326. https:\/\/doi.org\/10.1073\/pnas.84.12.4322","journal-title":"Proc Natl Acad Sci USA"},{"key":"2322_CR13","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.bbr.2009.06.022","volume":"205","author":"RJ Egan","year":"2009","unstructured":"Egan RJ, Bergner CL, Hart PC et al (2009) Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish. Behav Brain Res 205:38\u201344. https:\/\/doi.org\/10.1016\/j.bbr.2009.06.022","journal-title":"Behav Brain Res"},{"key":"2322_CR14","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1002\/cne.902260302","volume":"226","author":"P Ekstr\u00f6m","year":"1984","unstructured":"Ekstr\u00f6m P, Van Veen T (1984) Distribution of 5-hydroxytryptamine (serotonin) in the brain of the teleost Gasterosteus aculeatus L. J Comp Neurol 226:307\u2013320. https:\/\/doi.org\/10.1002\/cne.902260302","journal-title":"J Comp Neurol"},{"key":"2322_CR15","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.ygcen.2009.04.026","volume":"1","author":"J Falc\u00f3n","year":"2010","unstructured":"Falc\u00f3n J, Migaud H, Mu\u00f1oz-Cueto JA, Carrillo M (2010) Current knowledge on the melatonin system in teleost fish. Gen Comp Endocrinol 1:165. https:\/\/doi.org\/10.1016\/j.ygcen.2009.04.026","journal-title":"Gen Comp Endocrinol"},{"key":"2322_CR16","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1007\/BF00303149","volume":"169","author":"THM Frankenhuis-van den Heuvel","year":"1984","unstructured":"Frankenhuis-van den Heuvel THM, Nieuwenhuys R (1984) Distribution of serotonin-immunoreactivity in the diencephalon and mesencephalon of the trout, Salmo gairdneri\u2014Cellbodies, fibres and terminals. Anat Embryol (berl) 169:193\u2013204. https:\/\/doi.org\/10.1007\/BF00303149","journal-title":"Anat Embryol (berl)"},{"key":"2322_CR17","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1002\/cne.902460205","volume":"246","author":"M Fujimiya","year":"1986","unstructured":"Fujimiya M, Kimura H, Maeda T (1986) Postnatal development of serotonin nerve fibers in the somatosensory cortex of mice studied by immunohistochemistry. J Comp Neurol 246:191\u2013201. https:\/\/doi.org\/10.1002\/cne.902460205","journal-title":"J Comp Neurol"},{"key":"2322_CR18","doi-asserted-by":"publisher","first-page":"10387","DOI":"10.1523\/JNEUROSCI.1978-09.2009","volume":"29","author":"JP Gabriel","year":"2009","unstructured":"Gabriel JP, Mahmood R, Kyriakatos A et al (2009) Serotonergic modulation of locomotion in zebrafish\u2014Endogenous release and synaptic mechanisms. J Neurosci 29:10387\u201310395. https:\/\/doi.org\/10.1523\/JNEUROSCI.1978-09.2009","journal-title":"J Neurosci"},{"key":"2322_CR19","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1007\/s004410050812","volume":"288","author":"JM Garc\u00eda-Fern\u00e1ndez","year":"1997","unstructured":"Garc\u00eda-Fern\u00e1ndez JM, Jim\u00e9nez AJ, Gonz\u00e1lez B et al (1997) An immunocytochemical study of encephalic photoreceptors in three species of lamprey. Cell Tissue Res 288:267\u2013278. https:\/\/doi.org\/10.1007\/s004410050812","journal-title":"Cell Tissue Res"},{"key":"2322_CR20","doi-asserted-by":"publisher","first-page":"2382","DOI":"10.1098\/rstb.2011.0378","volume":"367","author":"P Gaspar","year":"2012","unstructured":"Gaspar P, Lillesaar C (2012) Probing the diversity of serotonin neurons. Philos Trans R Soc B Biol Sci 367:2382\u20132394. https:\/\/doi.org\/10.1098\/rstb.2011.0378","journal-title":"Philos Trans R Soc B Biol Sci"},{"key":"2322_CR21","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1111\/jne.12248","volume":"27","author":"M Gesto","year":"2015","unstructured":"Gesto M, L\u00f3pez-Pati\u00f1o MA, Hern\u00e1ndez J et al (2015) Gradation of the stress response in rainbow trout exposed to stressors of different severity: the role of brain serotonergic and dopaminergic systems. J Neuroendocrinol 27:131\u2013141. https:\/\/doi.org\/10.1111\/jne.12248","journal-title":"J Neuroendocrinol"},{"key":"2322_CR22","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/s0306-4522(98)00281-4","volume":"89","author":"SR Hansson","year":"1998","unstructured":"Hansson SR, Mezey E, Hoffman BJ (1998) Serotonin transporter messenger RNA expression in neural crest-derived structures and sensory pathways of the developing rat embryo. Neuroscience 89:243\u2013265. https:\/\/doi.org\/10.1016\/s0306-4522(98)00281-4","journal-title":"Neuroscience"},{"key":"2322_CR23","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.1098\/rspb.2000.1111","volume":"267","author":"A Hay-Schmidt","year":"2000","unstructured":"Hay-Schmidt A (2000) The evolution of the serotonergic nervous system. Proc R Soc B Biol Sci 267:1071\u20131079. https:\/\/doi.org\/10.1098\/rspb.2000.1111","journal-title":"Proc R Soc B Biol Sci"},{"key":"2322_CR24","doi-asserted-by":"publisher","first-page":"158","DOI":"10.3389\/fendo.2019.00158","volume":"10","author":"E H\u00f6glund","year":"2019","unstructured":"H\u00f6glund E, \u00d8verli \u00d8, Winberg S (2019) Tryptophan metabolic pathways and brain serotonergic activity: a comparative review. Front Endocrinol (lausanne) 10:158. https:\/\/doi.org\/10.3389\/fendo.2019.00158","journal-title":"Front Endocrinol (lausanne)"},{"key":"2322_CR25","doi-asserted-by":"publisher","first-page":"933","DOI":"10.1002\/cne.22739","volume":"520","author":"V Kuscha","year":"2012","unstructured":"Kuscha V, Barreiro-Iglesias A, Becker CG, Becker T (2012) Plasticity of tyrosine hydroxylase and serotonergic systems in the regenerating spinal cord of adult zebrafish. J Comp Neurol 520:933\u2013951. https:\/\/doi.org\/10.1002\/cne.22739","journal-title":"J Comp Neurol"},{"key":"2322_CR26","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1016\/s0896-6273(00)80215-9","volume":"17","author":"C Lebrand","year":"1996","unstructured":"Lebrand C, Cases O, Adelbrecht C, Doye A, Alvarez C, El Mestikawy S, Seif I, Gaspar P (1996) Transient uptake and storage of serotonin in developing thalamic neurons. Neuron 17:823\u2013835. https:\/\/doi.org\/10.1016\/s0896-6273(00)80215-9","journal-title":"Neuron"},{"key":"2322_CR27","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.yhbeh.2005.02.012","volume":"48","author":"O Lepage","year":"2005","unstructured":"Lepage O, Larson ET, Mayer I, Winberg S (2005) Serotonin, but not melatonin, plays a role in shaping dominant-subordinate relationships and aggression in rainbow trout. Horm Behav 48:233\u2013242. https:\/\/doi.org\/10.1016\/j.yhbeh.2005.02.012","journal-title":"Horm Behav"},{"key":"2322_CR28","doi-asserted-by":"publisher","first-page":"3679","DOI":"10.1242\/jeb.205.23.3679","volume":"205","author":"O Lepage","year":"2002","unstructured":"Lepage O, Tottmar O, Winberg S (2002) Elevated dietary intake of l-tryptophan counteracts the stress-induced elevation of plasma cortisol in rainbow trout (Oncorhynchus mykiss). J Exp Biol 205:3679\u20133687","journal-title":"J Exp Biol"},{"key":"2322_CR29","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1016\/j.jchemneu.2011.05.009","volume":"44","author":"C Lillesaar","year":"2011","unstructured":"Lillesaar C (2011) The serotonergic system in fish. J Chem Neuroanat 44:294\u2013308. https:\/\/doi.org\/10.1016\/j.jchemneu.2011.05.009","journal-title":"J Chem Neuroanat"},{"key":"2322_CR30","doi-asserted-by":"crossref","unstructured":"Lillesaar C, Gaspar P (2018) Serotonergic neurons in vertebrate and invertebrate model organisms (Rodents, zebrafish, drosophila melanogaster, aplysia californica, caenorhabditis elegans). In: Serotonin: The Mediator that Spans Evolution. pp 49\u201380","DOI":"10.1016\/B978-0-12-800050-2.00003-6"},{"key":"2322_CR31","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1002\/cne.21887","volume":"512","author":"C Lillesaar","year":"2009","unstructured":"Lillesaar C, Stigloher C, Tannh\u00e4user B et al (2009) Axonal projections originating from raphe serotonergic neurons in the developing and adult Zebrafish, Danio Rerio, using transgenics to visualize Raphe-specific pet1 expression. J Comp Neurol 512:158\u2013182. https:\/\/doi.org\/10.1002\/cne.21887","journal-title":"J Comp Neurol"},{"key":"2322_CR32","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1002\/dvdy.21095","volume":"236","author":"C Lillesaar","year":"2007","unstructured":"Lillesaar C, Tannh\u00e4user B, Stigloher C et al (2007) The serotonergic phenotype is acquired by converging genetic mechanisms within the zebrafish central nervous system. Dev Dyn 236:1072\u20131084. https:\/\/doi.org\/10.1002\/dvdy.21095","journal-title":"Dev Dyn"},{"key":"2322_CR33","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1159\/000505473","volume":"95","author":"D Lozano","year":"2020","unstructured":"Lozano D, Gonz\u00e1lez A, L\u00f3pez JM (2020) Neuroanatomical distribution of the serotonergic system in the brain and retina of holostean fishes, the sister group to teleosts. Brain Behav Evol 95:25\u201344. https:\/\/doi.org\/10.1159\/000505473","journal-title":"Brain Behav Evol"},{"key":"2322_CR34","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1159\/000067194","volume":"60","author":"PR Manger","year":"2002","unstructured":"Manger PR, Fahringer HM, Pettigrew JD, Siegel JM (2002) The distribution and morphological characteristics of serotonergic cells in the brain of monotremes. Brain Behav Evol 60:315\u2013332. https:\/\/doi.org\/10.1159\/000067194","journal-title":"Brain Behav Evol"},{"key":"2322_CR35","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1002\/cne.20280","volume":"480","author":"DL McLean","year":"2004","unstructured":"McLean DL, Fetcho JR (2004) Ontogeny and innervation patterns of dopaminergic, noradrenergic, and serotonergic neurons in larval zebrafish. J Comp Neurol 480:38\u201356. https:\/\/doi.org\/10.1002\/cne.20280","journal-title":"J Comp Neurol"},{"key":"2322_CR500","doi-asserted-by":"crossref","unstructured":"Norton WHJ, Folchert A, Bally-Cuif L (2008) Comparative analysis of serotonin receptor (HTR1A\/HTR1B Families) and transporter (slc6a4a\/b) gene expression in the zebrafish brain. J Com Neurol 511:521-542","DOI":"10.1002\/cne.21831"},{"key":"2322_CR36","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/j.pbb.2014.09.022","volume":"126","author":"M Nowicki","year":"2014","unstructured":"Nowicki M, Tran S, Muraleetharan A et al (2014) Serotonin antagonists induce anxiolytic and anxiogenic-like behavior in zebrafish in a receptor-subtype dependent manner. Pharmacol Biochem Behav 126:170\u2013180. https:\/\/doi.org\/10.1016\/j.pbb.2014.09.022","journal-title":"Pharmacol Biochem Behav"},{"key":"2322_CR37","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1016\/j.neuron.2019.05.038","volume":"103","author":"G Oikonomou","year":"2019","unstructured":"Oikonomou G, Altermatt M, Zhang R, wei, et al (2019) The serotonergic raphe promote sleep in zebrafish and mice. Neuron 103:686\u2013701. https:\/\/doi.org\/10.1016\/j.neuron.2019.05.038","journal-title":"Neuron"},{"key":"2322_CR38","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/j.yhbeh.2003.12.002","volume":"45","author":"\u00d8 \u00d8verli","year":"2004","unstructured":"\u00d8verli \u00d8, Korzan WJ, H\u00f6glund E et al (2004) Stress coping style predicts aggression and social dominance in rainbow trout. Horm Behav 45:235\u2013241. https:\/\/doi.org\/10.1016\/j.yhbeh.2003.12.002","journal-title":"Horm Behav"},{"key":"2322_CR39","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1093\/icb\/45.3.463","volume":"45","author":"\u00d8 \u00d8verli","year":"2005","unstructured":"\u00d8verli \u00d8, Winberg S, Pottinger TG (2005) Behavioral and neuroendocrine correlates of selection for stress responsiveness in rainbow trout\u2014a review. Integr Comp Biol 45:463\u2013474. https:\/\/doi.org\/10.1093\/icb\/45.3.463","journal-title":"Integr Comp Biol"},{"key":"2322_CR40","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.nbd.2010.05.010","volume":"40","author":"P Panula","year":"2010","unstructured":"Panula P, Chen YC, Priyadarshini M et al (2010) The comparative neuroanatomy and neurochemistry of zebrafish CNS systems of relevance to human neuropsychiatric diseases. Neurobiol Dis 40:46\u201357. https:\/\/doi.org\/10.1016\/j.nbd.2010.05.010","journal-title":"Neurobiol Dis"},{"key":"2322_CR41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cbpc.2013.12.003","volume":"161","author":"JJ P\u00e9rez-Maceira","year":"2014","unstructured":"P\u00e9rez-Maceira JJ, Mancebo MJ, Aldegunde M (2014) The involvement of 5-HT-like receptors in the regulation of food intake in rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol C Toxicol Pharmacol 161:1\u20136. https:\/\/doi.org\/10.1016\/j.cbpc.2013.12.003","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"key":"2322_CR42","doi-asserted-by":"publisher","first-page":"3292","DOI":"10.1111\/ejn.12348","volume":"38","author":"MR P\u00e9rez","year":"2013","unstructured":"P\u00e9rez MR, Pellegrini E, Cano-Nicolau J et al (2013) Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish\u2014potential effects of serotonin on adult neurogenesis. Eur J Neurosci 38:3292\u20133301. https:\/\/doi.org\/10.1111\/ejn.12348","journal-title":"Eur J Neurosci"},{"issue":"102","key":"2322_CR43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1095\/biolreprod.115.129965","volume":"93","author":"P Prasad","year":"2015","unstructured":"Prasad P, Ogawa S, Parhar IS (2015) Serotonin reuptake inhibitor citalopram inhibits gnrh synthesis and spermatogenesis in the male zebrafish. Biol Reprod 93(102):1\u201310. https:\/\/doi.org\/10.1095\/biolreprod.115.129965","journal-title":"Biol Reprod"},{"key":"2322_CR44","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1002\/jmor.20581","volume":"277","author":"L Rinc\u00f3n Camacho","year":"2016","unstructured":"Rinc\u00f3n Camacho L, Morandini L, Birba A et al (2016) The pineal complex: a morphological and immunohistochemical comparison between a tropical (Paracheirodon axelrodi) and a subtropical (Aphyocharax anisitsi) characid species. J Morphol 277:1355\u20131367. https:\/\/doi.org\/10.1002\/jmor.20581","journal-title":"J Morphol"},{"key":"2322_CR45","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/S0006-8993(00)03174-7","volume":"889","author":"E Rink","year":"2001","unstructured":"Rink E, Wullimann MF (2001) The teleostean (zebrafish) dopaminergic system ascending to the subpallium (striatum) is located in the basal diencephalon (posterior tuberculum). Brain Res 889:316\u2013330. https:\/\/doi.org\/10.1016\/S0006-8993(00)03174-7","journal-title":"Brain Res"},{"key":"2322_CR46","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/S0891-0618(99)00049-6","volume":"18","author":"FJ Rodr\u00edguez-G\u00f3mez","year":"2000","unstructured":"Rodr\u00edguez-G\u00f3mez FJ, Rend\u00f3n-Unceta MC, Sarasquete C, Mu\u00f1oz-Cueto JA (2000) Distribution of serotonin in the brain of the Senegalese sole, Solea senegalensis: an immunohistochemical study. J Chem Neuroanat 18:103\u2013115. https:\/\/doi.org\/10.1016\/S0891-0618(99)00049-6","journal-title":"J Chem Neuroanat"},{"key":"2322_CR47","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.physbeh.2005.08.030","volume":"87","author":"VC Rubio","year":"2006","unstructured":"Rubio VC, S\u00e1nchez-V\u00e1zquez FJ, Madrid JA (2006) Oral serotonin administration affects the quantity and the quality of macronutrients selection in European sea bass Dicentrarchus labrax L. Physiol Behav 87:7\u201315. https:\/\/doi.org\/10.1016\/j.physbeh.2005.08.030","journal-title":"Physiol Behav"},{"key":"2322_CR48","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1085\/JGP.201812066","volume":"151","author":"G Rudnick","year":"2019","unstructured":"Rudnick G, Sandtner W (2019) Serotonin transport in the 21st century. J Gen Physiol 151:1248\u20131264. https:\/\/doi.org\/10.1085\/JGP.201812066","journal-title":"J Gen Physiol"},{"key":"2322_CR49","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1007\/s00359-002-0320-z","volume":"188","author":"C Ruibal","year":"2002","unstructured":"Ruibal C, Soengas JL, Aldegunde M (2002) Brain serotonin and the control of food intake in rainbow trout (Oncorhynchus mykiss): effects of changes in plasma glucose levels. J Comp Physiol A Neuroethol Sensory, Neural, Behav Physiol 188:479\u2013484. https:\/\/doi.org\/10.1007\/s00359-002-0320-z","journal-title":"J Comp Physiol A Neuroethol Sensory, Neural, Behav Physiol"},{"key":"2322_CR50","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1002\/cne.902530106","volume":"253","author":"H Sako","year":"1986","unstructured":"Sako H, Kojima T, Okado N (1986) Immunohistochemical study on the development of serotoninergic neurons in the chick: I. Distribution of cell bodies and fibers in the brain. J Comp Neurol 253:61\u201378. https:\/\/doi.org\/10.1002\/cne.902530106","journal-title":"J Comp Neurol"},{"key":"2322_CR51","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1111\/j.1471-4159.2008.05793.x","volume":"108","author":"V Sallinen","year":"2009","unstructured":"Sallinen V, Torkko V, Sundvik M et al (2009) MPTP and MPP+ target specific aminergic cell populations in larval zebrafish. J Neurochem 108:719\u2013731. https:\/\/doi.org\/10.1111\/j.1471-4159.2008.05793.x","journal-title":"J Neurochem"},{"key":"2322_CR52","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1016\/j.physbeh.2006.02.009","volume":"87","author":"J Schjolden","year":"2006","unstructured":"Schjolden J, Pulman KGT, Pottinger TG et al (2006) Serotonergic characteristics of rainbow trout divergent in stress responsiveness. Physiol Behav 87:938\u2013947. https:\/\/doi.org\/10.1016\/j.physbeh.2006.02.009","journal-title":"Physiol Behav"},{"key":"2322_CR53","doi-asserted-by":"publisher","first-page":"44777","DOI":"10.1038\/srep44777","volume":"7","author":"I Shainer","year":"2017","unstructured":"Shainer I, Buchshtab A, Hawkins TA, Wilson SW, Cone RD, Gothilf Y (2017) Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish. Sci Rep 7:44777. https:\/\/doi.org\/10.1038\/srep44777","journal-title":"Sci Rep"},{"key":"2322_CR54","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1111\/jfb.13757","volume":"93","author":"MC Soares","year":"2018","unstructured":"Soares MC, Gerlai R, Maximino C (2018) The integration of sociality, monoamines and stress neuroendocrinology in fish models: applications in the neurosciences. J Fish Biol 93:170\u2013191. https:\/\/doi.org\/10.1111\/jfb.13757","journal-title":"J Fish Biol"},{"key":"2322_CR600","doi-asserted-by":"crossref","unstructured":"Sobrido-Came\u00e1n D, Robledo D, S\u00e1nchez L, Rodicio MC, Barreiro-Iglesias A (2019) Serotonin inhibits axonal regeneration of identifiable descending neurons after a complete spinal cord injury in lampreys. Dis Model Mech 12(2):dmm037085","DOI":"10.1242\/dmm.037085"},{"key":"2322_CR55","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1002\/neu.20023","volume":"60","author":"H Teraoka","year":"2004","unstructured":"Teraoka H, Russell C, Regan J et al (2004) Hedgehog and Fgf signaling pathways regulate the development of tphR-expressing serotonergic raphe neurons in zebrafish embryos. J Neurobiol 60:275\u2013288. https:\/\/doi.org\/10.1002\/neu.20023","journal-title":"J Neurobiol"},{"key":"2322_CR56","doi-asserted-by":"publisher","first-page":"3451","DOI":"10.1002\/cne.24938","volume":"528","author":"M Timothy","year":"2020","unstructured":"Timothy M, Forlano PM (2020) Serotonin distribution in the brain of the plainfin midshipman: Substrates for vocal-acoustic modulation and a reevaluation of the serotonergic system in teleost fishes. J Comp Neurol 528:3451\u20133478. https:\/\/doi.org\/10.1002\/cne.24938","journal-title":"J Comp Neurol"},{"key":"2322_CR57","doi-asserted-by":"publisher","first-page":"30","DOI":"10.3389\/fncir.2016.00030","volume":"10","author":"KJ Turner","year":"2016","unstructured":"Turner KJ, Hawkins TA, Y\u00e1\u00f1ez J et al (2016) Afferent connectivity of the zebrafish habenulae. Front Neural Circuits 10:30. https:\/\/doi.org\/10.3389\/fncir.2016.00030","journal-title":"Front Neural Circuits"},{"key":"2322_CR58","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/0736-5748(86)90028-6","volume":"4","author":"P Van Mier","year":"1986","unstructured":"Van Mier P, Joosten HWJ, Van Rheden R, Donkelaar HJT (1986) The development of serotonergic raphespinal projections in Xenopus laevis. Int J Dev Neurosci 4:465\u2013475. https:\/\/doi.org\/10.1016\/0736-5748(86)90028-6","journal-title":"Int J Dev Neurosci"},{"key":"2322_CR59","doi-asserted-by":"publisher","first-page":"e85543","DOI":"10.1371\/journal.pone.0085543","volume":"9","author":"MA Vindas","year":"2014","unstructured":"Vindas MA, S\u00f8rensen C, Johansen IB et al (2014) Coping with unpredictability: Dopaminergic and neurotrophic responses to omission of expected reward in Atlantic salmon (Salmo salar L). PLoS ONE 9:e85543. https:\/\/doi.org\/10.1371\/journal.pone.0085543","journal-title":"PLoS ONE"},{"key":"2322_CR60","doi-asserted-by":"publisher","first-page":"1673","DOI":"10.1016\/S0006-2952(03)00556-2","volume":"66","author":"DJ Walther","year":"2003","unstructured":"Walther DJ, Bader M (2003) A unique central tryptophan hydroxylase isoform. Biochem Pharmacol 66:1673\u20131680. https:\/\/doi.org\/10.1016\/S0006-2952(03)00556-2","journal-title":"Biochem Pharmacol"},{"key":"2322_CR61","doi-asserted-by":"publisher","first-page":"3867","DOI":"10.1242\/jeb.204.22.3867","volume":"204","author":"S Winberg","year":"2001","unstructured":"Winberg S, \u00d8verli \u00d8, Lepage O (2001) Suppression of aggression in rainbow trout (Oncorhynchus mykiss) by dietary l-tryptophan. J Exp Biol 204:3867\u20133876","journal-title":"J Exp Biol"},{"key":"2322_CR62","doi-asserted-by":"crossref","unstructured":"Wullimann MF, Rupp B, Reichert H (1996) Neuroanatomy of the Zebrafish Brain","DOI":"10.1007\/978-3-0348-8979-7"},{"key":"2322_CR63","doi-asserted-by":"publisher","first-page":"203","DOI":"10.3390\/genes10030203","volume":"10","author":"J Xu","year":"2019","unstructured":"Xu J, Li Y, Lv Y et al (2019) Molecular evolution of tryptophan hydroxylases in vertebrates: a comparative genomic survey. Genes (basel) 10:203. https:\/\/doi.org\/10.3390\/genes10030203","journal-title":"Genes (basel)"},{"key":"2322_CR64","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/j.neures.2006.10.004","volume":"57","author":"HG Xue","year":"2007","unstructured":"Xue HG, Yang CY, Yamamoto N, Ozawa H (2007) Fiber connections of the periventricular pretectal nucleus in a teleost, tilapia (Oreochromis niloticus). Neurosci Res 57:184\u2013193. https:\/\/doi.org\/10.1016\/j.neures.2006.10.004","journal-title":"Neurosci Res"},{"key":"2322_CR65","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1016\/j.mcn.2010.01.006","volume":"43","author":"K Yamamoto","year":"2010","unstructured":"Yamamoto K, Ruuskanen JO, Wullimann MF, Vernier P (2010) Two tyrosine hydroxylase genes in vertebrates. New dopaminergic territories revealed in the zebrafish brain. Mol Cell Neurosci 43:394\u2013402. https:\/\/doi.org\/10.1016\/j.mcn.2010.01.006","journal-title":"Mol Cell Neurosci"},{"key":"2322_CR66","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.8765","volume":"8","author":"M Ziegler","year":"2020","unstructured":"Ziegler M, Knoll S, K\u00f6hler HR et al (2020) Impact of the antidepressant citalopram on the behaviour of two different life stages of brown trout. PeerJ 8:e8765. https:\/\/doi.org\/10.7717\/peerj.8765","journal-title":"PeerJ"}],"container-title":["Brain Structure and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00429-021-02322-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00429-021-02322-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00429-021-02322-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T13:30:02Z","timestamp":1628602202000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00429-021-02322-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,2]]},"references-count":68,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["2322"],"URL":"https:\/\/doi.org\/10.1007\/s00429-021-02322-8","relation":{},"ISSN":["1863-2653","1863-2661"],"issn-type":[{"value":"1863-2653","type":"print"},{"value":"1863-2661","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,2]]},"assertion":[{"value":"8 February 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 June 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Authors have nothing to declare.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All experiments were carried out in accordance with the principles published in the European animal directive (86\/609\/EEC) for the protection of experimental animals and approved by the Consejo Superior de Investigaciones Cient\u00edficas (CSIC) ethics committee (project number AGL2016-74857-C3-3-R and PID2019-103969RB-C33).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"No applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Studies involving humans and\/or animals"}},{"value":"No applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"No applicable.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}]}}