{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:25:20Z","timestamp":1789730720352,"version":"4.0.1"},"reference-count":69,"publisher":"Springer Science and Business Media LLC","issue":"3-4","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Ministerio de Educacion y Ciencia, Spain","award":["BES-2016-077912"],"award-info":[{"award-number":["BES-2016-077912"]}]},{"DOI":"10.13039\/100020230","name":"Consejer\u00eda de Transformaci\u00f3n Econ\u00f3mica, Industria, Conocimiento y Universidades","doi-asserted-by":"publisher","award":["P20_00529"],"award-info":[{"award-number":["P20_00529"]}],"id":[{"id":"10.13039\/100020230","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020230","name":"Consejer\u00eda de Transformaci\u00f3n Econ\u00f3mica, Industria, Conocimiento y Universidades","doi-asserted-by":"publisher","award":["P20_00529"],"award-info":[{"award-number":["P20_00529"]}],"id":[{"id":"10.13039\/100020230","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MCIN\/AEI\/FEDER","award":["PGC2018-094654-B-100"],"award-info":[{"award-number":["PGC2018-094654-B-100"]}]},{"name":"MCIN\/AEI\/FEDER","award":["PGC2018-094654-B-100"],"award-info":[{"award-number":["PGC2018-094654-B-100"]}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01 NS111969"],"award-info":[{"award-number":["R01 NS111969"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Brain Struct Funct"],"DOI":"10.1007\/s00429-023-02635-w","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T01:57:57Z","timestamp":1680487077000},"page":"967-984","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Preservation of KCC2 expression in axotomized abducens motoneurons and its enhancement by VEGF"],"prefix":"10.1007","volume":"228","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9817-8728","authenticated-orcid":false,"given":"Paula M.","family":"Calvo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5301-5108","authenticated-orcid":false,"given":"Rosa R.","family":"de la Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6213-7454","authenticated-orcid":false,"given":"Angel M.","family":"Pastor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7011-3244","authenticated-orcid":false,"given":"Francisco J.","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,4,1]]},"reference":[{"key":"2635_CR1","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1242\/dev.00351","volume":"130","author":"F Aguado","year":"2003","unstructured":"Aguado F, Carmona MA, Pozas E, Aguil\u00f3 A, Mart\u00ednez-Guijarro FJ, Alcantara S, Borrell V, Yuste R, Iba\u00f1ez CF, Soriano E (2003) BDNF regulates spontaneous correlated activity at early developmental stages by increasing synaptogenesis and expression of the K+\/Cl- co-transporter KCC2. Development 130:1267\u20131280. https:\/\/doi.org\/10.1242\/dev.00351","journal-title":"Development"},{"key":"2635_CR2","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1523\/eneuro.0172-19.2019","volume":"6","author":"ET Akhter","year":"2019","unstructured":"Akhter ET, Griffith RW, English AW, Alvarez FJ (2019) Removal of the potassium chloride co-transporter from the somatodendritic membrane of axotomized motoneurons is independent of BDNF\/TrkB signaling but is controlled by neuromuscular innervation. eNeuro 6:5. https:\/\/doi.org\/10.1523\/eneuro.0172-19.2019","journal-title":"eNeuro"},{"key":"2635_CR3","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1007\/s00424-020-02375-4","volume":"472","author":"T Akita","year":"2020","unstructured":"Akita T, Fukuda A (2020) Intracellular Cl - dysregulation causing and caused by pathogenic neuronal activity. Pflugers Arch 472:977\u2013987. https:\/\/doi.org\/10.1007\/s00424-020-02375-4","journal-title":"Pflugers Arch"},{"key":"2635_CR5","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1038\/nature02544","volume":"429","author":"M Azzouz","year":"2004","unstructured":"Azzouz M, Ralph GS, Storkebaum E, Walmsley LE, Mitrophanous KA, Kingsman SM, Carmeliet P, Mazarakis ND (2004) VEGF delivery with retrogradely transported lentivector prolongs survival in a mouse ALS model. Nature 429:413\u2013417. https:\/\/doi.org\/10.1038\/nature02544","journal-title":"Nature"},{"key":"2635_CR6","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.neuroscience.2014.08.001","volume":"279","author":"Y Ben-Ari","year":"2014","unstructured":"Ben-Ari Y (2014) The GABA excitatory\/inhibitory developmental sequence: a personal journey. Neuroscience 279:187\u2013219. https:\/\/doi.org\/10.1016\/j.neuroscience.2014.08.001","journal-title":"Neuroscience"},{"key":"2635_CR7","doi-asserted-by":"publisher","first-page":"2016","DOI":"10.3390\/ijms17122016","volume":"17","author":"B Ben\u00edtez-Temi\u00f1o","year":"2016","unstructured":"Ben\u00edtez-Temi\u00f1o B, Davis-L\u00f3pez de Carrizosa MA, Morcuende S, Matarredona ER, de la Cruz RR, Pastor AM (2016) Functional diversity of neurotrophin actions on the oculomotor system. Int J Mol Sci 17:2016. https:\/\/doi.org\/10.3390\/ijms17122016","journal-title":"Int J Mol Sci"},{"key":"2635_CR8","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1089\/neu.2019.6526","volume":"37","author":"H Beverungen","year":"2020","unstructured":"Beverungen H, Klaszky SC, Klaszky M, C\u00f4t\u00e9 MP (2020) Rehabilitation decreases spasticity by restoring chloride homeostasis through the brain-derived neurotrophic factor-KCC2 pathway after spinal cord injury. J Neurotrauma 37:846\u2013859. https:\/\/doi.org\/10.1089\/neu.2019.6526","journal-title":"J Neurotrauma"},{"key":"2635_CR9","doi-asserted-by":"publisher","first-page":"113605","DOI":"10.1016\/j.expneurol.2021.113605","volume":"338","author":"JN Bilchak","year":"2021","unstructured":"Bilchak JN, Yeakle K, Caron G, Malloy D, C\u00f4t\u00e9 MP (2021) Enhancing KCC2 activity decreases hyperreflexia and spasticity after chronic spinal cord injury. Exp Neurol 338:113605. https:\/\/doi.org\/10.1016\/j.expneurol.2021.113605","journal-title":"Exp Neurol"},{"key":"2635_CR10","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1073\/pnas.1213680110","volume":"110","author":"R Bos","year":"2013","unstructured":"Bos R, Sadlaoud K, Boulenguez P, Buttigieg D, Liabeuf S, Brocard C, Haase G, Bras H, Vinay L (2013) Activation of 5-HT2A receptors upregulates the function of the neuronal K-Cl cotransporter KCC2. Proc Natl Acad Sci USA 110:348\u2013353. https:\/\/doi.org\/10.1073\/pnas.1213680110","journal-title":"Proc Natl Acad Sci USA"},{"key":"2635_CR11","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1038\/nm.2107","volume":"16","author":"P Boulenguez","year":"2010","unstructured":"Boulenguez P, Liabeuf S, Bos R, Bras H, Jean-Xavier C, C\u00e9cile Brocard C, Stil A, Darbon P, Cattaert D, Delpire E, Marsala M, Vinay L (2010) Down-regulation of the potassium-chloride cotransporter KCC2 contributes to spasticity after spinal cord injury. Nat Med 16:302\u2013307. https:\/\/doi.org\/10.1038\/nm.2107","journal-title":"Nat Med"},{"key":"2635_CR12","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.expneurol.2018.03.004","volume":"304","author":"PM Calvo","year":"2018","unstructured":"Calvo PM, de la Cruz RR, Pastor AM (2018) Synaptic loss and firing alterations in axotomized motoneurons are restored by vascular endothelial growth factor (VEGF) and VEGF-B. Exp Neurol 304:67\u201381. https:\/\/doi.org\/10.1016\/j.expneurol.2018.03.004","journal-title":"Exp Neurol"},{"key":"2635_CR13","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1523\/eneuro.0467-19.2020","volume":"7","author":"PM Calvo","year":"2020","unstructured":"Calvo PM, de la Cruz RR, Pastor AM (2020) A single intraventricular injection of VEGF leads to long-term neurotrophic effects in axotomized motoneurons. eNeuro 7:3. https:\/\/doi.org\/10.1523\/eneuro.0467-19.2020","journal-title":"eNeuro"},{"key":"2635_CR14","doi-asserted-by":"publisher","first-page":"5","DOI":"10.3389\/fncel.2012.00005","volume":"6","author":"I Chamma","year":"2012","unstructured":"Chamma I, Chevy Q, Poncer JC, L\u00e9vi S (2012) Role of the neuronal K-Cl co-transporter KCC2 in inhibitory and excitatory neurotransmission. Front Cell Neurosci 6:5. https:\/\/doi.org\/10.3389\/fncel.2012.00005","journal-title":"Front Cell Neurosci"},{"key":"2635_CR15","doi-asserted-by":"publisher","first-page":"48","DOI":"10.3389\/fncel.2019.00048","volume":"13","author":"E C\u00f4me","year":"2019","unstructured":"C\u00f4me E, Heubl M, Schwartz EJ, Poncer JC, L\u00e9vi S (2019) Reciprocal regulation of KCC2 trafficking and synaptic activity. Front Cell Neurosci 13:48. https:\/\/doi.org\/10.3389\/fncel.2019.00048","journal-title":"Front Cell Neurosci"},{"key":"2635_CR16","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1186\/1744-8069-4-36","volume":"4","author":"SW Cramer","year":"2008","unstructured":"Cramer SW, Baggott C, Cain J, Tilghman J, Allcock B, Miranpuri G, Rajpal S, Sun D, Resnick D (2008) The role of cation-dependent chloride transporters in neuropathic pain following spinal cord injury. Mol Pain 4:36. https:\/\/doi.org\/10.1186\/1744-8069-4-36","journal-title":"Mol Pain"},{"key":"2635_CR17","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/j.jneumeth.2007.08.021","volume":"167","author":"MA Davis-L\u00f3pez de Carrizosa","year":"2008","unstructured":"Davis-L\u00f3pez de Carrizosa MA, Tena JJ, Ben\u00edtez-Temi\u00f1o B, Morado-D\u00edaz CJ, Pastor AM, de la Cruz RR (2008) A chronically implantable device for the controlled delivery of substances, and stimulation and recording of activity in severed nerves. J Neurosci Methods 167:302\u2013309. https:\/\/doi.org\/10.1016\/j.jneumeth.2007.08.021","journal-title":"J Neurosci Methods"},{"key":"2635_CR18","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1523\/jneurosci.5312-08.2009","volume":"29","author":"MA Davis-L\u00f3pez de Carrizosa","year":"2009","unstructured":"Davis-L\u00f3pez de Carrizosa MA, Morado-D\u00edaz CJ, Tena JJ, Ben\u00edtez-Temi\u00f1o B, Pecero ML, Morcuende SR, de la Cruz RR, Pastor AM (2009) Complementary actions of BDNF and neurotrophin-3 on the firing patterns and synaptic composition of motoneurons. J Neurosci 29:575\u2013587. https:\/\/doi.org\/10.1523\/jneurosci.5312-08.2009","journal-title":"J Neurosci"},{"key":"2635_CR19","doi-asserted-by":"publisher","first-page":"8308","DOI":"10.1523\/jneurosci.0719-10.2010","volume":"30","author":"MA Davis-L\u00f3pez de Carrizosa","year":"2010","unstructured":"Davis-L\u00f3pez de Carrizosa MA, Morado-D\u00edaz CJ, Morcuende S, de la Cruz RR, Pastor AM (2010) Nerve growth factor regulates the firing patterns and synaptic composition of motoneurons. J Neurosci 30:8308\u20138319. https:\/\/doi.org\/10.1523\/jneurosci.0719-10.2010","journal-title":"J Neurosci"},{"key":"2635_CR20","doi-asserted-by":"publisher","first-page":"2271","DOI":"10.1523\/jneurosci.5416-10.2011","volume":"31","author":"MA Davis-L\u00f3pez de Carrizosa","year":"2011","unstructured":"Davis-L\u00f3pez de Carrizosa MA, Morado-D\u00edaz CJ, Miller JM, de la Cruz RR, Pastor AM (2011) Dual encoding of muscle tension and eye position by abducens motoneurons. J Neurosci 31:2271\u20132279. https:\/\/doi.org\/10.1523\/jneurosci.5416-10.2011","journal-title":"J Neurosci"},{"key":"2635_CR21","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1002\/(sici)1096-9861(19980119)390:3%3C377::aid-cne6%3E3.0.co;2-z","volume":"390","author":"RR de la Cruz","year":"1998","unstructured":"de la Cruz RR, Pastor AM, Mart\u00ednez-Guijarro FJ, L\u00f3pez-Garc\u00eda C, Delgado-Garc\u00eda JM (1998) Localization of parvalbumin, calretinin, and calbindin D-28k in identified extraocular motoneurons and internuclear neurons of the cat. J Comp Neurol 390:377\u2013391. https:\/\/doi.org\/10.1002\/(sici)1096-9861(19980119)390:3%3C377::aid-cne6%3E3.0.co;2-z","journal-title":"J Comp Neurol"},{"key":"2635_CR22","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1016\/0306-4522(86)90072-2","volume":"17","author":"JM Delgado-Garc\u00eda","year":"1986","unstructured":"Delgado-Garc\u00eda JM, del Pozo F, Baker R (1986a) Behavior of neurons in the abducens nucleus of the alert cat-I. Motoneurons Neuroscience 17:929\u2013952. https:\/\/doi.org\/10.1016\/0306-4522(86)90072-2","journal-title":"Motoneurons Neuroscience"},{"key":"2635_CR23","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1016\/0306-4522(86)90073-4","volume":"17","author":"JM Delgado-Garc\u00eda","year":"1986","unstructured":"Delgado-Garc\u00eda JM, del Pozo F, Baker R (1986b) Behavior of neurons in the abducens nucleus of the alert cat-II. Internuclear Neurons Neuroscience 17:953\u2013973. https:\/\/doi.org\/10.1016\/0306-4522(86)90073-4","journal-title":"Internuclear Neurons Neuroscience"},{"key":"2635_CR24","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/0306-4522(88)90319-3","volume":"24","author":"JM Delgado-Garc\u00eda","year":"1988","unstructured":"Delgado-Garc\u00eda JM, Del Pozo F, Spencer RF, Baker R (1988) Behavior of neurons in the abducens nucleus of the alert cat-III. Axotomized Motoneurons Neurosci 24:143\u2013160. https:\/\/doi.org\/10.1016\/0306-4522(88)90319-3","journal-title":"Axotomized Motoneurons Neurosci"},{"issue":"1","key":"2635_CR25","doi-asserted-by":"publisher","first-page":"ENEURO.0281-16.","DOI":"10.1523\/ENEURO.0281-16.2016","volume":"4","author":"SS Dukkipati","year":"2017","unstructured":"Dukkipati SS, Chihi A, Wang Y, Elbasiouny SM (2017) Experimental design and data analysis issues contribute to inconsistent results of C-bouton changes in amyotrophic lateral sclerosis. eNeuro 4(1):ENEURO.0281-16.2016. https:\/\/doi.org\/10.1523\/ENEURO.0281-16.2016","journal-title":"eNeuro"},{"key":"2635_CR26","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1007\/bf00247538","volume":"71","author":"M Escudero","year":"1988","unstructured":"Escudero M, Delgado-Garc\u00eda JM (1988) Behavior of reticular, vestibular and prepositus neurons terminating in the abducens nucleus of the alert cat. Exp Brain Res 71:218\u2013222. https:\/\/doi.org\/10.1007\/bf00247538","journal-title":"Exp Brain Res"},{"key":"2635_CR27","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1113\/jphysiol.1992.sp019433","volume":"458","author":"M Escudero","year":"1992","unstructured":"Escudero M, de la Cruz RR, Delgado-Garc\u00eda JM (1992) A physiological study of vestibular and prepositus hypoglossi neurones projecting to the abducens nucleus in the alert cat. J Physiol 458:539\u2013560. https:\/\/doi.org\/10.1113\/jphysiol.1992.sp019433","journal-title":"J Physiol"},{"key":"2635_CR28","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1097\/nen.0b013e3181f4dcef","volume":"10","author":"A Fuchs","year":"2010","unstructured":"Fuchs A, Ringer C, Bilkei-Gorzo A, Weihe E, Roeper J, Sch\u00fctz B (2010) Downregulation of the potassium chloride cotransporter KCC2 in vulnerable motoneurons in the SOD1-G93A mouse model of amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 10:1057\u20131070. https:\/\/doi.org\/10.1097\/nen.0b013e3181f4dcef","journal-title":"J Neuropathol Exp Neurol"},{"key":"2635_CR29","doi-asserted-by":"publisher","first-page":"1524","DOI":"10.1038\/nm.3356","volume":"19","author":"M Gagnon","year":"2013","unstructured":"Gagnon M, Bergeron MJ, Lavertu G, Castonguay A, Tripathy S, Bonin RP, Perez-Sanchez J, Boudreau D, Wang B, Dumas L, Valade I, Bachand K, Jacob-Wagner M, Tardif C, Kianicka I, Isenring P, Attardo G, Coull JA, De Koninck Y (2013) Chloride extrusion enhancers as novel therapeutics for neurological diseases. Nat Med 19:1524\u20131528. https:\/\/doi.org\/10.1038\/nm.3356","journal-title":"Nat Med"},{"key":"2635_CR30","doi-asserted-by":"publisher","first-page":"160091","DOI":"10.1098\/rsob.160091","volume":"6","author":"M Gey","year":"2016","unstructured":"Gey M, Wanner R, Schilling C, Pedro MT, Sinske D, Kn\u00f6ll B (2016) Atf3 mutant mice show reduced axon regeneration and impaired regeneration-associated gene induction after peripheral nerve injury. Open Biol 6:160091. https:\/\/doi.org\/10.1098\/rsob.160091","journal-title":"Open Biol"},{"key":"2635_CR31","doi-asserted-by":"publisher","first-page":"1628","DOI":"10.1042\/bst20110720","volume":"39","author":"T Gustafsson","year":"2011","unstructured":"Gustafsson T (2011) Vascular remodelling in human skeletal muscle. Biochem Soc Trans 39:1628\u20131632. https:\/\/doi.org\/10.1042\/bst20110720","journal-title":"Biochem Soc Trans"},{"key":"2635_CR32","doi-asserted-by":"publisher","first-page":"10661","DOI":"10.1074\/jbc.274.15.10661","volume":"274","author":"K Hiki","year":"1999","unstructured":"Hiki K, D\u2019Andrea RJ, Furze J, Crawford J, Woollatt E, Sutherland GR, Vadas MA, Gamble JR (1999) Cloning, characterization, and chromosomal location of a novel human K+-Cl- cotransporter. J Biol Chem 274:10661\u2013106617","journal-title":"J Biol Chem"},{"key":"2635_CR33","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1038\/s41592-019-0470-3","volume":"16","author":"J Ho","year":"2019","unstructured":"Ho J, Tumkaya T, Aryal S, Choi H, Claridge-Chang A (2019) Moving beyond P values: data analysis with estimation graphics. Nat Methods 16:565\u2013566. https:\/\/doi.org\/10.1038\/s41592-019-0470-3","journal-title":"Nat Methods"},{"key":"2635_CR34","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1074\/jbc.274.15.10661","volume":"21","author":"B Hoier","year":"2014","unstructured":"Hoier B, Hellsten Y (2014) Exercise-induced capillary growth in human skeletal muscle and the dynamics of VEGF. Microcirculation 21:301\u2013314. https:\/\/doi.org\/10.1074\/jbc.274.15.10661","journal-title":"Microcirculation"},{"key":"2635_CR35","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1523\/eneuro.0025-19.2019","volume":"6","author":"SD Holland","year":"2019","unstructured":"Holland SD, Ramer LM, McMahon SB, Denk F, Ramer MS (2019) An ATF3-creert2 knock-in mouse for axotomy-induced genetic editing: proof of principle. eNeuro 6:2. https:\/\/doi.org\/10.1523\/eneuro.0025-19.2019","journal-title":"eNeuro"},{"key":"2635_CR36","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1001\/jamaneurol.2014.21","volume":"71","author":"KT Kahle","year":"2014","unstructured":"Kahle KT, Khanna A, Clapham DE, Woolf CJ (2014) Therapeutic restoration of spinal inhibition via druggable enhancement of potassium-chloride cotransporter KCC2-mediated chloride extrusion in peripheral neuropathic pain. JAMA Neurol 71:640\u2013645. https:\/\/doi.org\/10.1001\/jamaneurol.2014.21","journal-title":"JAMA Neurol"},{"key":"2635_CR37","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1038\/nrn3819","volume":"15","author":"K Kaila","year":"2014","unstructured":"Kaila K, Price TJ, Payne JA, Puskarjov M, Voipio J (2014) Cation-chloride cotransporters in neuronal development, plasticity and disease. Nat Rev Neurosci 15:637\u2013654. https:\/\/doi.org\/10.1038\/nrn3819","journal-title":"Nat Rev Neurosci"},{"key":"2635_CR38","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.jchemneu.2017.11.002","volume":"88","author":"J Kim","year":"2018","unstructured":"Kim J, Kobayashi S, Shimizu-Okabe C, Okabe A, Moon C, Shin T, Takayama CJ (2018) Changes in the expression and localization of signaling molecules in mouse facial motor neurons during regeneration of facial nerves. Chem Neuroanat 88:13\u201321. https:\/\/doi.org\/10.1016\/j.jchemneu.2017.11.002","journal-title":"Chem Neuroanat"},{"key":"2635_CR39","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1038\/nn.2806","volume":"14","author":"HH Lee","year":"2011","unstructured":"Lee HH, Deeb TZ, Walker JA, Davies PA, Moss SJ (2011) NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci 14:736\u2013743. https:\/\/doi.org\/10.1038\/nn.2806","journal-title":"Nat Neurosci"},{"key":"2635_CR40","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.neuint.2019.04.003","volume":"128","author":"S Lee-Hotta","year":"2019","unstructured":"Lee-Hotta S, Uchiyama Y, Kametaka S (2019) Role of the BDNF-TrkB pathway in KCC2 regulation and rehabilitation following neuronal injury: a mini review. Neurochem Int 128:32\u201338","journal-title":"Neurochem Int"},{"key":"2635_CR41","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1038\/s41467-019-14154-6","volume":"11","author":"LE Lorenzo","year":"2020","unstructured":"Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, Ribeiro-da-Silva A, De Koninck Y (2020) Enhancing neuronal chloride extrusion rescues \u03b12\/\u03b13 GABAA-mediated analgesia in neuropathic pain. Nat Commun 11:869. https:\/\/doi.org\/10.1038\/s41467-019-14154-6","journal-title":"Nat Commun"},{"key":"2635_CR42","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1523\/jneurosci.2006-10.2011","volume":"31","author":"A Ludwig","year":"2011","unstructured":"Ludwig A, Uvarov P, Soni S, Thomas-Crusells J, Airaksinen MS, Rivera CJ (2011) Early growth response 4 mediates BDNF induction of potassium chloride cotransporter 2 transcription. J Neurosci 31:644\u2013649. https:\/\/doi.org\/10.1523\/jneurosci.2006-10.2011","journal-title":"J Neurosci"},{"key":"2635_CR43","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1002\/cne.23510","volume":"522","author":"M Markkanen","year":"2014","unstructured":"Markkanen M, Karhunen T, Llano O, Ludwig A, Rivera C, Uvarov P, Airaksinen MS (2014) Distribution of neuronal KCC2a and KCC2b isoforms in mouse CNS. J Comp Neurol 522:1897\u20131914. https:\/\/doi.org\/10.1002\/cne.23510","journal-title":"J Comp Neurol"},{"key":"2635_CR44","doi-asserted-by":"publisher","first-page":"27","DOI":"10.3389\/fncel.2014.00027","volume":"8","author":"I Medina","year":"2014","unstructured":"Medina I, Friedel P, Rivera C, Kahle KT, Kourdougli N, Uvarov P, Pellegrino C (2014) Current view on the functional regulation of the neuronal K(+)-Cl(-) cotransporter KCC2. Front Cell Neurosci 8:27. https:\/\/doi.org\/10.3389\/fncel.2014.00027","journal-title":"Front Cell Neurosci"},{"key":"2635_CR45","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1016\/j.pain.2007.10.016","volume":"137","author":"G Miletic","year":"2008","unstructured":"Miletic G, Miletic V (2008) Loose ligation of the sciatic nerve is associated with TrkB receptor-dependent decreases in KCC2 protein levels in the ipsilateral spinal dorsal horn. Pain 137:532\u2013539. https:\/\/doi.org\/10.1016\/j.pain.2007.10.016","journal-title":"Pain"},{"key":"2635_CR46","doi-asserted-by":"publisher","first-page":"10166","DOI":"10.1073\/pnas.1810134115","volume":"115","author":"YE Moore","year":"2018","unstructured":"Moore YE, Deeb TZ, Chadchankar H, Brandon NJ, Moss SJ (2018) Potentiating KCC2 activity is sufficient to limit the onset and severity of seizures. Proc Natl Acad Sci USA 115:10166\u201310171. https:\/\/doi.org\/10.1073\/pnas.1810134115","journal-title":"Proc Natl Acad Sci USA"},{"key":"2635_CR47","doi-asserted-by":"publisher","first-page":"7007","DOI":"10.1523\/jneurosci.4231-13.2014","volume":"34","author":"CJ Morado-D\u00edaz","year":"2014","unstructured":"Morado-D\u00edaz CJ, Matarredona ER, Morcuende S, Talaver\u00f3n R, Davis-L\u00f3pez de Carrizosa MA, de la Cruz RR, Pastor AM (2014) Neural progenitor cell implants in the lesioned medial longitudinal fascicle of adult cats regulate synaptic composition and firing properties of abducens internuclear neurons. J Neurosci 34:7007\u20137017. https:\/\/doi.org\/10.1523\/jneurosci.4231-13.2014","journal-title":"J Neurosci"},{"key":"2635_CR48","doi-asserted-by":"publisher","first-page":"4412","DOI":"10.1523\/jneurosci.22-11-04412.2002","volume":"22","author":"J Nabekura","year":"2002","unstructured":"Nabekura J, Ueno T, Okabe A, Furuta A, Iwaki T, Shimizu-Okabe C, Fukuda A, Akaike N (2002) Reduction of KCC2 expression and GABAA receptor-mediated excitation after in vivo axonal injury. J Neurosci 22:4412\u20134417. https:\/\/doi.org\/10.1523\/jneurosci.22-11-04412.2002","journal-title":"J Neurosci"},{"key":"2635_CR49","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1038\/88842","volume":"28","author":"B Oosthuyse","year":"2001","unstructured":"Oosthuyse B, Moons L, Storkebaum E et al (2001) Deletion of the hypoxia-response element in the vascular endothelial growth factor promoter causes motor neuron degeneration. Nat Genet 28:131\u2013138. https:\/\/doi.org\/10.1038\/88842","journal-title":"Nat Genet"},{"key":"2635_CR50","doi-asserted-by":"publisher","first-page":"8465","DOI":"10.1073\/pnas.0602979103","volume":"103","author":"E Palma","year":"2006","unstructured":"Palma E, Amici M, Sobrero F, Spinelli G, Di Angelantonio S, Ragozzino D, Mascia A, Scoppetta C, Esposito V, Miledi R, Eusebi F (2006) Anomalous levels of Cl- transporters in the hippocampal subiculum from temporal lobe epilepsy patients make GABA excitatory. Proc Natl Acad Sci USA 103:8465\u20138468. https:\/\/doi.org\/10.1073\/pnas.0602979103","journal-title":"Proc Natl Acad Sci USA"},{"key":"2635_CR51","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.neuroscience.2008.03.072","volume":"154","author":"E Papp","year":"2008","unstructured":"Papp E, Rivera C, Kaila K, Freund TF (2008) Relationship between neuronal vulnerability and potassium-chloride cotransporter 2 immunoreactivity in hippocampus following transient forebrain ischemia. Neuroscience 154:677\u2013689. https:\/\/doi.org\/10.1016\/j.neuroscience.2008.03.072","journal-title":"Neuroscience"},{"key":"2635_CR52","doi-asserted-by":"publisher","first-page":"8","DOI":"10.3389\/fnmol.2012.00008","volume":"5","author":"S Patodia","year":"2012","unstructured":"Patodia S, Raivich G (2012) Role of transcription factors in peripheral nerve regeneration. Front Mol Neurosci 5:8. https:\/\/doi.org\/10.3389\/fnmol.2012.00008","journal-title":"Front Mol Neurosci"},{"key":"2635_CR53","doi-asserted-by":"publisher","first-page":"16245","DOI":"10.1074\/jbc.271.27.16245","volume":"271","author":"JA Payne","year":"1996","unstructured":"Payne JA, Stevenson TJ, Donaldson LF (1996) Molecular characterization of a putative K-Cl cotransporter in rat brain. A neuronal-specific isoform. J Biol Chem 271:16245\u201316252. https:\/\/doi.org\/10.1074\/jbc.271.27.16245","journal-title":"J Biol Chem"},{"key":"2635_CR54","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/s0166-2236(03)00068-7","volume":"26","author":"JA Payne","year":"2003","unstructured":"Payne JA, Rivera C, Voipio J, Kaila K (2003) Cation-chloride co-transporters in neuronal communication, development and trauma. Trends Neurosci 26:199\u2013206. https:\/\/doi.org\/10.1016\/s0166-2236(03)00068-7","journal-title":"Trends Neurosci"},{"key":"2635_CR55","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.neubiorev.2021.01.024","volume":"124","author":"C Peerboom","year":"2021","unstructured":"Peerboom C, Wierenga CJ (2021) The postnatal GABA shift: a developmental perspective. Neurosci Biobehav Rev 124:179\u2013192. https:\/\/doi.org\/10.1016\/j.neubiorev.2021.01.024","journal-title":"Neurosci Biobehav Rev"},{"key":"2635_CR56","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1038\/s41398-020-01027-6","volume":"10","author":"D Pozzi","year":"2020","unstructured":"Pozzi D, Rasile M, Corradini I, Matteoli M (2020) Environmental regulation of the chloride transporter KCC2: switching inflammation off to switch the GABA on? Transl Psychiatry 10:349. https:\/\/doi.org\/10.1038\/s41398-020-01027-6","journal-title":"Transl Psychiatry"},{"key":"2635_CR57","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1038\/16697","volume":"397","author":"C Rivera","year":"1999","unstructured":"Rivera C, Voipio J, Payne JA, Ruusuvuori E, Lahtinen H, Lamsa K, Pirvola U, Saarma M, Kaila K (1999) The K+\/Cl- co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation. Nature 397:251\u2013255. https:\/\/doi.org\/10.1038\/16697","journal-title":"Nature"},{"key":"2635_CR58","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1083\/jcb.200209011","volume":"159","author":"C Rivera","year":"2002","unstructured":"Rivera C, Li H, Thomas-Crusells J, Lahtinen H, Viitanen T, Nanobashvili A, Kokaia Z, Airaksinen MS, Voipio J, Kaila K, Saarma M (2002) BDNF-induced TrkB activation down-regulates the K+-Cl- cotransporter KCC2 and impairs neuronal Cl- extrusion. J Cell Biol 159:747\u2013752. https:\/\/doi.org\/10.1083\/jcb.200209011","journal-title":"J Cell Biol"},{"key":"2635_CR59","doi-asserted-by":"publisher","first-page":"4683","DOI":"10.1523\/jneurosci.5265-03.2004","volume":"24","author":"C Rivera","year":"2004","unstructured":"Rivera C, Voipio J, Thomas-Crusells J, Li H, Emri Z, Sipil\u00e4 S, Payne JA, Minichiello L, Saarma M, Kaila K (2004) Mechanism of activity-dependent downregulation of the neuron-specific K-Cl cotransporter KCC2. J Neurosci 24:4683\u20134691. https:\/\/doi.org\/10.1523\/jneurosci.5265-03.2004","journal-title":"J Neurosci"},{"key":"2635_CR60","doi-asserted-by":"publisher","first-page":"7911","DOI":"10.1523\/jneurosci.5313-06.2007","volume":"27","author":"R Seijffers","year":"2007","unstructured":"Seijffers R, Mills CD, Woolf CJ (2007) ATF3 increases the intrinsic growth state of DRG neurons to enhance peripheral nerve regeneration. J Neurosci 27:7911\u20137920. https:\/\/doi.org\/10.1523\/jneurosci.5313-06.2007","journal-title":"J Neurosci"},{"key":"2635_CR61","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1007\/s00429-020-02057-y","volume":"225","author":"S Silva-Hucha","year":"2020","unstructured":"Silva-Hucha S, Carrero-Rojas G, Fern\u00e1ndez de Sevilla ME, Ben\u00edtez-Temi\u00f1o B, Davis-L\u00f3pez de Carrizosa MA, Pastor AM, Morcuende S (2020) Sources and lesion-induced changes of VEGF expression in brainstem motoneurons. Brain Struct Funct 225:1033\u20131053. https:\/\/doi.org\/10.1007\/s00429-020-02057-y","journal-title":"Brain Struct Funct"},{"key":"2635_CR62","doi-asserted-by":"publisher","first-page":"e54519","DOI":"10.1371\/journal.pone.0054519","volume":"8","author":"R Talaver\u00f3n","year":"2013","unstructured":"Talaver\u00f3n R, Matarredona ER, de la Cruz RR, Pastor AM (2013) Neural progenitor cell implants modulate vascular endothelial growth factor and brain-derived neurotrophic factor expression in rat axotomized neurons. PLoS ONE 8:e54519. https:\/\/doi.org\/10.1371\/journal.pone.0054519","journal-title":"PLoS ONE"},{"key":"2635_CR63","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.brainres.2012.01.048","volume":"1446","author":"M Tatetsu","year":"2012","unstructured":"Tatetsu M, Kim J, Kina S, Sunakawa H, Takayama C (2012) GABA\/glycine signaling during degeneration and regeneration of mouse hypoglossal nerves. Brain Res 1446:22\u201333. https:\/\/doi.org\/10.1016\/j.brainres.2012.01.048","journal-title":"Brain Res"},{"key":"2635_CR64","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1097\/nen.0b013e3181567c16","volume":"66","author":"LB Tovar-y-Romo","year":"2007","unstructured":"Tovar-y-Romo LB, Zepeda A, Tapia R (2007) Vascular endothelial growth factor prevents paralysis and motoneuron death in a rat model of excitotoxic spinal cord neurodegeneration. J Neuropathol Exp Neurol 66:913\u2013922. https:\/\/doi.org\/10.1097\/nen.0b013e3181567c16","journal-title":"J Neuropathol Exp Neurol"},{"key":"2635_CR65","doi-asserted-by":"publisher","first-page":"1353","DOI":"10.1152\/jn.00721.2002","volume":"89","author":"H Toyoda","year":"2003","unstructured":"Toyoda H, Ohno K, Yamada J, Ikeda M, Okabe A, Sato K, Hashimoto K, Fukuda A (2003) Induction of NMDA and GABAA receptor-mediated Ca2+ oscillations with KCC2 mRNA downregulation in injured facial motoneurons. J Neurophysiol 89:1353\u20131362. https:\/\/doi.org\/10.1152\/jn.00721.2002","journal-title":"J Neurophysiol"},{"key":"2635_CR66","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1006\/mcne.1999.0814","volume":"15","author":"H Tsujino","year":"2000","unstructured":"Tsujino H, Kondo E, Fukuoka T, Dai Y, Tokunaga A, Miki K, Yonenobu K, Ochi T, Noguchi K (2000) Activating transcription factor 3 (ATF3) induction by axotomy in sensory and motoneurons: a novel neuronal marker of nerve injury. Mol Cell Neurosci 15:170\u2013182. https:\/\/doi.org\/10.1006\/mcne.1999.0814","journal-title":"Mol Cell Neurosci"},{"key":"2635_CR67","doi-asserted-by":"publisher","first-page":"30570","DOI":"10.1074\/jbc.m705095200","volume":"282","author":"P Uvarov","year":"2007","unstructured":"Uvarov P, Ludwig A, Markkanen M, Pruunsild P, Kaila K, Delpire E, Timmusk T, Rivera C, Airaksinen MS (2007) A novel N-terminal isoform of the neuron-specific K-Cl cotransporter KCC2. J Biol Chem 282:30570\u201330576. https:\/\/doi.org\/10.1074\/jbc.m705095200","journal-title":"J Biol Chem"},{"key":"2635_CR68","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1002\/hipo.10014","volume":"12","author":"N-S Woo","year":"2002","unstructured":"Woo N-S, Lu J, England R, McClellan R, Dufour S, Mount DB, Deutch AY, Lovinger DM, Delpire E (2002) Hyperexcitability and epilepsy associated with disruption of the mouse neuronal-specific K-Cl cotransporter gene. Hippocampus 12:258\u2013268. https:\/\/doi.org\/10.1002\/hipo.10014","journal-title":"Hippocampus"},{"key":"2635_CR69","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1038\/nrn2336","volume":"9","author":"S Zacchigna","year":"2008","unstructured":"Zacchigna S, Lambrechts D, Carmeliet P (2008) Neurovascular signaling defects in neurodegeneration. Nat Rev Neurosci 9:169\u2013181. https:\/\/doi.org\/10.1038\/nrn2336","journal-title":"Nat Rev Neurosci"},{"key":"2635_CR70","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.eplepsyres.2008.02.005","volume":"79","author":"L Zhu","year":"2008","unstructured":"Zhu L, Polley N, Mathews GC, Delpire E (2008) NKCC1 and KCC2 prevent hyperexcitability in the mouse hippocampus. Epilepsy Res 79:201\u2013212. https:\/\/doi.org\/10.1016\/j.eplepsyres.2008.02.005","journal-title":"Epilepsy Res"}],"container-title":["Brain Structure and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00429-023-02635-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00429-023-02635-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00429-023-02635-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T11:06:20Z","timestamp":1682679980000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00429-023-02635-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,1]]},"references-count":69,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["2635"],"URL":"https:\/\/doi.org\/10.1007\/s00429-023-02635-w","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-2252183\/v1","asserted-by":"object"}]},"ISSN":["1863-2661"],"issn-type":[{"value":"1863-2661","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,1]]},"assertion":[{"value":"8 November 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 March 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 April 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have no financial or non-financial interests to disclose.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All animal procedures were performed at the University of Seville (Spain) and in accordance with the guidelines of the European Union (2010\/63\/EU) and Spanish legislation (R.D. 53\/2013, BOE 34\/11370-421) for the use and care of laboratory animals. They were approved by the local ethics committee (Protocol #04\/11\/15\/349). Animal procedures also followed NIH guidelines and legislation in the US. No animal experimentation was performed in the US for this project. Work at the US (Emory University) consisted in immunocytochemical and morphological analyses of tissues collected at University of Seville in Spain. All efforts were made to reduce the number of animals used and their suffering during the present experiments and, in fact, some of the material derives from the tissue bank of previously published studies (Calvo et al.\n                      \n                      ,\n                      \n                      ).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}