{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T17:16:18Z","timestamp":1756574178995,"version":"3.40.3"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T00:00:00Z","timestamp":1536710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T00:00:00Z","timestamp":1536710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Med Microbiol Immunol"],"published-print":{"date-parts":[[2019,2,4]]},"DOI":"10.1007\/s00430-018-0559-9","type":"journal-article","created":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T14:53:58Z","timestamp":1536764038000},"page":"89-100","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Intranasal co-administration of recombinant active fragment of Zonula occludens toxin and truncated recombinant EspB triggers potent systemic, mucosal immune responses and reduces span of E. coli O157:H7 fecal shedding in BALB\/c mice"],"prefix":"10.1007","volume":"208","author":[{"given":"Aravind","family":"Shekar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shylaja","family":"Ramlal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph Kingston","family":"Jeyabalaji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murali Harishchandra","family":"Sripathy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,9,12]]},"reference":[{"key":"559_CR1","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/j.vetmic.2009.04.011","volume":"140","author":"MA Karmali","year":"2010","unstructured":"Karmali MA, Gannon V, Sargeant JM (2010) Verocytotoxin-producing Escherichia coli (VTEC). Vet Microbiol 140:360\u2013370","journal-title":"Vet Microbiol"},{"key":"559_CR2","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1111\/j.1863-2378.2012.01523.x","volume":"60","author":"NP Varela","year":"2013","unstructured":"Varela NP, Dick P, Wilson J (2013) Assessing the existing information on the efficacy of bovine vaccination against Escherichia coli o157: H7\u2014a systematic review and meta-analysis. Zoonoses Public Health 60:253\u2013268","journal-title":"Zoonoses Public Health"},{"key":"559_CR3","doi-asserted-by":"publisher","first-page":"16265","DOI":"10.1073\/pnas.1304978110","volume":"110","author":"L Matthews","year":"2013","unstructured":"Matthews L, Reeve R, Gally DL et al (2013) Predicting the public health benefit of vaccinating cattle against Escherichia coli O157. Proc Natl Acad Sci USA 110:16265\u201316270. https:\/\/doi.org\/10.1073\/pnas.1304978110","journal-title":"Proc Natl Acad Sci USA"},{"key":"559_CR4","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/j.vaccine.2003.08.007","volume":"22","author":"AA Potter","year":"2004","unstructured":"Potter AA, Klashinsky S, Li Y et al (2004) Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins. Vaccine 22:362\u2013369. https:\/\/doi.org\/10.1016\/j.vaccine.2003.08.007","journal-title":"Vaccine"},{"key":"559_CR5","doi-asserted-by":"publisher","first-page":"1422","DOI":"10.1016\/j.vaccine.2009.10.076","volume":"28","author":"TN McNeilly","year":"2010","unstructured":"McNeilly TN, Mitchell MC, Rosser T et al (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge. Vaccine 28:1422\u20131428. https:\/\/doi.org\/10.1016\/j.vaccine.2009.10.076","journal-title":"Vaccine"},{"key":"559_CR6","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.micpath.2017.01.062","volume":"105","author":"R Lin","year":"2017","unstructured":"Lin R, Zhu B, Zhang Y et al (2017) Intranasal immunization with novel EspA-Tir-M fusion protein induces protective immunity against enterohemorrhagic Escherichia coli O157:H7 challenge in mice. Microb Pathog 105:19\u201324. https:\/\/doi.org\/10.1016\/j.micpath.2017.01.062","journal-title":"Microb Pathog"},{"key":"559_CR7","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.1348-0421.2011.00316.x","volume":"55","author":"C Wan","year":"2011","unstructured":"Wan C, Zhou Y, Yu Y et al (2011) B-cell epitope KT-12 of enterohemorrhagic Escherichia coli O157:H7: a novel peptide vaccine candidate. Microbiol Immunol 55:247\u2013253. https:\/\/doi.org\/10.1111\/j.1348-0421.2011.00316.x","journal-title":"Microbiol Immunol"},{"key":"559_CR8","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1111\/j.1574-695X.2007.00341.x","volume":"52","author":"P Nart","year":"2008","unstructured":"Nart P, Holden N, McAteer SP et al (2008) Mucosal antibody responses of colonized cattle to Escherichia coli O157-secreted proteins, flagellin, outer membrane proteins and lipopolysaccharide. FEMS Immunol Med Microbiol 52:59\u201368. https:\/\/doi.org\/10.1111\/j.1574-695X.2007.00341.x","journal-title":"FEMS Immunol Med Microbiol"},{"key":"559_CR9","doi-asserted-by":"publisher","first-page":"5242","DOI":"10.1073\/pnas.88.12.5242","volume":"88","author":"A Fasano","year":"1991","unstructured":"Fasano A, Baudry B, Pumplin DW et al (1991) Vibrio cholerae produces a second enterotoxin, which affects intestinal tight junctions. Proc Natl Acad Sci USA 88:5242\u20135246. https:\/\/doi.org\/10.1073\/pnas.88.12.5242","journal-title":"Proc Natl Acad Sci USA"},{"key":"559_CR10","doi-asserted-by":"publisher","first-page":"1158","DOI":"10.1172\/JCI119271","volume":"99","author":"A Fasano","year":"1997","unstructured":"Fasano A, Uzzau S (1997) Modulation of intestinal tight junctions by zonula occludens toxin permits enteral administration of insulin and other macromolecules in an animal model. J Clin Invest 99:1158\u20131164. https:\/\/doi.org\/10.1172\/JCI119271","journal-title":"J Clin Invest"},{"key":"559_CR11","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1172\/JCI118114","volume":"96","author":"A Fasano","year":"1995","unstructured":"Fasano A, Fiorentini C, Donelli G et al (1995) Zonula occludens toxin modulates tight junctions through protein kinase C-dependent actin reorganization, in vitro. J Clin Invest 96:710\u2013720. https:\/\/doi.org\/10.1172\/JCI118114","journal-title":"J Clin Invest"},{"key":"559_CR12","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1128\/IAI.71.4.1897-1902.2003","volume":"71","author":"M Marinaro","year":"2003","unstructured":"Marinaro M, Fasano A, De Magistris MT (2003) Zonula occludens toxin acts as an adjuvant through different mucosal routes and induces protective immune responses. Infect Immun 71:1897\u20131902. https:\/\/doi.org\/10.1128\/IAI.71.4.1897-1902.2003","journal-title":"Infect Immun"},{"key":"559_CR13","doi-asserted-by":"publisher","first-page":"19160","DOI":"10.1074\/jbc.M009674200","volume":"276","author":"M Di Pierro","year":"2001","unstructured":"Di Pierro M, Lu R, Uzzau S et al (2001) Zonula occludens toxin structure-function analysis. Identification of the fragment biologically active on tight junctions and of the zonulin receptor binding domain. J Biol Chem 276:19160\u201319165. https:\/\/doi.org\/10.1074\/jbc.M009674200","journal-title":"J Biol Chem"},{"key":"559_CR14","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1124\/jpet.104.073205","volume":"312","author":"NN Salama","year":"2005","unstructured":"Salama NN, Fasano A, Thakar M, Eddington ND (2005) The impact of \u2206G on the oral bioavailability of low bioavailable therapeutic agents. J Pharmacol Exp Ther 312:199\u2013205. https:\/\/doi.org\/10.1124\/jpet.104.073205","journal-title":"J Pharmacol Exp Ther"},{"key":"559_CR15","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.vaccine.2011.11.003","volume":"30","author":"V Pavot","year":"2012","unstructured":"Pavot V, Rochereau N, Genin C et al (2012) New insights in mucosal vaccine development. Vaccine 30:142\u2013154. https:\/\/doi.org\/10.1016\/j.vaccine.2011.11.003","journal-title":"Vaccine"},{"key":"559_CR16","doi-asserted-by":"publisher","first-page":"2409","DOI":"10.1111\/j.1742-4658.2010.07655.x","volume":"277","author":"D Hamada","year":"2010","unstructured":"Hamada D, Hamaguchi M, Suzuki KN et al (2010) Cytoskeleton-modulating effectors of enteropathogenic and enterohemorrhagic Escherichia coli: a case for EspB as an intrinsically less-ordered effector. FEBS J 277:2409\u20132415. https:\/\/doi.org\/10.1111\/j.1742-4658.2010.07655.x","journal-title":"FEBS J"},{"key":"559_CR17","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1046\/j.1365-2958.1998.01144.x","volume":"30","author":"G Frankel","year":"1998","unstructured":"Frankel G, Phillips AD, Rosenshine I et al (1998) Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol 30:911\u2013921. https:\/\/doi.org\/10.1046\/j.1365-2958.1998.01144.x","journal-title":"Mol Microbiol"},{"key":"559_CR18","doi-asserted-by":"publisher","first-page":"7991","DOI":"10.1073\/pnas.92.17.7991","volume":"92","author":"B Kenny","year":"1995","unstructured":"Kenny B, Finlay BB (1995) Protein secretion by enteropathogenic Escherichia coli is essential for transducing signals to epithelial cells. Proc Natl Acad Sci USA 92:7991\u20137995","journal-title":"Proc Natl Acad Sci USA"},{"key":"559_CR19","doi-asserted-by":"publisher","first-page":"1052","DOI":"10.1128\/CVI.00068-11","volume":"18","author":"DJ Asper","year":"2011","unstructured":"Asper DJ, Karmali MA, Townsend H et al (2011) Serological response of Shiga toxin-producing Escherichia coli type III secreted proteins in sera from vaccinated rabbits, naturally infected cattle, and humans. Clin Vaccine Immunol 18:1052\u20131057. https:\/\/doi.org\/10.1128\/CVI.00068-11","journal-title":"Clin Vaccine Immunol"},{"key":"559_CR20","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.gene.2012.08.011","volume":"509","author":"PK Reddy","year":"2012","unstructured":"Reddy PK, Ramlal S, Sripathy MH, Batra H (2012) A simple and universal ligation mediated fusion of genes based on hetero-staggered PCR for generating immunodominant chimeric proteins. Gene 509:104\u2013109. https:\/\/doi.org\/10.1016\/j.gene.2012.08.011","journal-title":"Gene"},{"key":"559_CR21","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.pep.2005.09.027","volume":"46","author":"P Reichelt","year":"2006","unstructured":"Reichelt P, Schwarz C, Donzeau M (2006) Single step protocol to purify recombinant proteins with low endotoxin contents. Protein Expr Purif 46:483\u2013488. https:\/\/doi.org\/10.1016\/j.pep.2005.09.027","journal-title":"Protein Expr Purif"},{"key":"559_CR22","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.imlet.2017.10.013","volume":"192","author":"G Tadepalli","year":"2017","unstructured":"Tadepalli G, Konduru B, Murali HS, Batra HV (2017) Intraperitoneal administration of a novel chimeric immunogen (rOP) elicits IFN-\u03b3 and IL-12p70 protective immune response in BALB\/c mice against virulent Brucella. Immunol Lett 192:79\u201387. https:\/\/doi.org\/10.1016\/j.imlet.2017.10.013","journal-title":"Immunol Lett"},{"key":"559_CR23","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1016\/j.plantsci.2011.01.004","volume":"180","author":"J Amani","year":"2011","unstructured":"Amani J, Mousavi SL, Rafati S, Salmanian AH (2011) Immunogenicity of a plant-derived edible chimeric EspA, Intimin and Tir of Escherichia coli O157:H7 in mice. Plant Sci 180:620\u2013627. https:\/\/doi.org\/10.1016\/j.plantsci.2011.01.004","journal-title":"Plant Sci"},{"key":"559_CR24","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.toxicon.2015.06.008","volume":"103","author":"PN Reddy","year":"2015","unstructured":"Reddy PN, Paul S, Sripathy MH, Batra HV (2015) Evaluation of recombinant SEA-TSST fusion toxoid for protection against superantigen induced toxicity in mouse model. Toxicon 103:106\u2013113. https:\/\/doi.org\/10.1016\/j.toxicon.2015.06.008","journal-title":"Toxicon"},{"key":"559_CR25","doi-asserted-by":"publisher","first-page":"e0169422","DOI":"10.1371\/journal.pone.0169422","volume":"12","author":"L Martorelli","year":"2017","unstructured":"Martorelli L, Garbaccio S, Vilte DA et al (2017) Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2b subunit of Escherichia coli O157:H7. PLoS One 12:e0169422. https:\/\/doi.org\/10.1371\/journal.pone.0169422","journal-title":"PLoS One"},{"key":"559_CR26","doi-asserted-by":"publisher","first-page":"e0121623","DOI":"10.1371\/journal.pone.0121623","volume":"10","author":"X Ruan","year":"2015","unstructured":"Ruan X, Sack DA, Zhang W (2015) Genetic fusions of a CFA\/I\/II\/IV MEFA (multiepitope fusion antigen) and a toxoid fusion of heat-stable toxin (STa) and heat-labile toxin (LT) of enterotoxigenic Escherichia coli (ETEC) retain broad anti-CFA and antitoxin antigenicity. PLoS One 10:e0121623. https:\/\/doi.org\/10.1371\/journal.pone.0121623","journal-title":"PLoS One"},{"key":"559_CR27","doi-asserted-by":"publisher","first-page":"6923","DOI":"10.1016\/j.vaccine.2010.07.061","volume":"28","author":"J Amani","year":"2010","unstructured":"Amani J, Salmanian AH, Rafati S, Mousavi SL (2010) Immunogenic properties of chimeric protein from espA, eae and tir genes of Escherichia coli O157:H7. Vaccine 28:6923\u20136929. https:\/\/doi.org\/10.1016\/j.vaccine.2010.07.061","journal-title":"Vaccine"},{"key":"559_CR28","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1111\/j.1863-2378.2011.01426.x","volume":"59","author":"KG Snedeker","year":"2012","unstructured":"Snedeker KG, Campbell M, Sargeant JM (2012) A systematic review of vaccinations to reduce the shedding of Escherichia coli O157 in the faeces of domestic ruminants. Zoonoses Public Health 59:126\u2013138. https:\/\/doi.org\/10.1111\/j.1863-2378.2011.01426.x","journal-title":"Zoonoses Public Health"},{"key":"559_CR29","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.vetimm.2010.11.010","volume":"140","author":"MA Yekta","year":"2011","unstructured":"Yekta MA, Goddeeris BM, Vanrompay D, Cox E (2011) Immunization of sheep with a combination of intimin\u03b3, EspA and EspB decreases Escherichia coli O157:H7 shedding. Vet Immunol Immunopathol 140:42\u201346. https:\/\/doi.org\/10.1016\/j.vetimm.2010.11.010","journal-title":"Vet Immunol Immunopathol"},{"key":"559_CR30","doi-asserted-by":"publisher","first-page":"3962","DOI":"10.1016\/j.vaccine.2011.03.079","volume":"29","author":"DA Vilte","year":"2011","unstructured":"Vilte DA, Larz\u00e1bal M, Garbaccio S et al (2011) Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with \u03b3-intimin C280 and EspB proteins. Vaccine 29:3962\u20133968. https:\/\/doi.org\/10.1016\/j.vaccine.2011.03.079","journal-title":"Vaccine"},{"key":"559_CR31","doi-asserted-by":"publisher","first-page":"5662","DOI":"10.1016\/j.vaccine.2008.07.027","volume":"26","author":"A Cataldi","year":"2008","unstructured":"Cataldi A, Yevsa T, Vilte DA et al (2008) Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2\/6 agonist MALP-2 as adjuvant. Vaccine 26:5662\u20135667. https:\/\/doi.org\/10.1016\/j.vaccine.2008.07.027","journal-title":"Vaccine"},{"key":"559_CR32","doi-asserted-by":"publisher","first-page":"3909","DOI":"10.1016\/j.vaccine.2014.05.054","volume":"32","author":"B Ahmed","year":"2014","unstructured":"Ahmed B, Loos M, Vanrompay D, Cox E (2014) Oral immunization with Lactococcus lactis-expressing EspB induces protective immune responses against Escherichia coli O157:H7 in a murine model of colonization. Vaccine 32:3909\u20133916. https:\/\/doi.org\/10.1016\/j.vaccine.2014.05.054","journal-title":"Vaccine"},{"key":"559_CR33","doi-asserted-by":"publisher","first-page":"417","DOI":"10.3389\/fmicb.2017.00417","volume":"8","author":"R Lin","year":"2017","unstructured":"Lin R, Zhang Y, Long B et al (2017) Oral immunization with recombinant lactobacillus acidophilus expressing espA-Tir-M confers protection against enterohemorrhagic Escherichia coli O157:H7 challenge in mice. Front Microbiol 8:417. https:\/\/doi.org\/10.3389\/fmicb.2017.00417","journal-title":"Front Microbiol"},{"key":"559_CR34","doi-asserted-by":"publisher","first-page":"3277","DOI":"10.1099\/mic.0.28115-0","volume":"151","author":"H-J Chiu","year":"2005","unstructured":"Chiu H-J, Syu W-J (2005) Functional analysis of EspB from enterohaemorrhagic Escherichia coli. Microbiology 151:3277\u20133286. https:\/\/doi.org\/10.1099\/mic.0.28115-0","journal-title":"Microbiology"},{"key":"559_CR35","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/j.ijmm.2018.03.005","volume":"308","author":"D Baumann","year":"2018","unstructured":"Baumann D, Salia H, Greune L et al (2018) Multitalented EspB of enteropathogenic Escherichia coli (EPEC) enters cells autonomously and induces programmed cell death in human monocytic THP-1 cells. Int J Med Microbiol 308:387\u2013404. https:\/\/doi.org\/10.1016\/j.ijmm.2018.03.005","journal-title":"Int J Med Microbiol"},{"key":"559_CR36","doi-asserted-by":"publisher","first-page":"3218","DOI":"10.1128\/IAI.72.6.3218-3227.2004","volume":"72","author":"VK Viswanathan","year":"2004","unstructured":"Viswanathan VK, Koutsouris A, Lukic S et al (2004) Comparative analysis of EspF from enteropathogenic and enterohemorrhagic Escherichia coli in alteration of epithelial barrier function. Infect Immun 72:3218\u20133227. https:\/\/doi.org\/10.1128\/IAI.72.6.3218-3227.2004","journal-title":"Infect Immun"},{"key":"559_CR37","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/j.immuni.2011.02.002","volume":"34","author":"H Tezuka","year":"2011","unstructured":"Tezuka H, Abe Y, Asano J et al (2011) Prominent role for plasmacytoid dendritic cells in mucosal T cell-independent IgA induction. Immunity 34:247\u2013257. https:\/\/doi.org\/10.1016\/j.immuni.2011.02.002","journal-title":"Immunity"},{"key":"559_CR38","doi-asserted-by":"publisher","first-page":"1871","DOI":"10.1084\/jem.20122762","volume":"210","author":"D Ruane","year":"2013","unstructured":"Ruane D, Brane L, Reis BS et al (2013) Lung dendritic cells induce migration of protective T cells to the gastrointestinal tract. J Exp Med 210:1871\u20131888. https:\/\/doi.org\/10.1084\/jem.20122762","journal-title":"J Exp Med"},{"key":"559_CR39","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1111\/j.1749-6632.1996.tb21115.x","volume":"778","author":"M Yamamoto","year":"1996","unstructured":"Yamamoto M, Vancott JL, Okahashi N et al (1996) The role of Th1 and Th2 cells for mucosal IgA responses. Ann N Y Acad Sci 778:64\u201371. https:\/\/doi.org\/10.1111\/j.1749-6632.1996.tb21115.x","journal-title":"Ann N Y Acad Sci"},{"key":"559_CR40","doi-asserted-by":"crossref","first-page":"4303","DOI":"10.4049\/jimmunol.138.12.4303","volume":"138","author":"LM Sollid","year":"1987","unstructured":"Sollid LM, Kvale D, Brandtzaeg P et al (1987) Interferon-gamma enhances expression of secretory component, the epithelial receptor for polymeric immunoglobulins. J Immunol (Baltimore Md 1950) 138:4303\u20134306","journal-title":"J Immunol (Baltimore Md 1950)"},{"key":"559_CR41","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2018.06.007","author":"C R\u00fcter","year":"2018","unstructured":"R\u00fcter C, Lubos ML, Norkowski S, Schmidt MA (2018) All in\u2014Multiple parallel strategies for intracellular delivery by bacterial pathogens. Int J Med Microbiol. https:\/\/doi.org\/10.1016\/j.ijmm.2018.06.007","journal-title":"Int J Med Microbiol"},{"key":"559_CR42","doi-asserted-by":"publisher","first-page":"366","DOI":"10.4161\/viru.24642","volume":"4","author":"AI Etcheverr\u00eda","year":"2013","unstructured":"Etcheverr\u00eda AI, Padola NL (2013) Shiga toxin-producing Escherichia coli: factors involved in virulence and cattle colonization. Virulence 4:366\u2013372","journal-title":"Virulence"},{"key":"559_CR43","doi-asserted-by":"publisher","first-page":"3949","DOI":"10.1016\/j.vaccine.2018.05.059","volume":"36","author":"L Martorelli","year":"2018","unstructured":"Martorelli L, Garimano N, Fiorentino GA et al (2018) Efficacy of a recombinant Intimin, EspB and Shiga toxin 2B vaccine in calves experimentally challenged with Escherichia coli O157:H7. Vaccine 36:3949\u20133959","journal-title":"Vaccine"},{"key":"559_CR44","doi-asserted-by":"publisher","first-page":"2016","DOI":"10.1128\/IAI.01437-13","volume":"82","author":"VA Garc\u00eda-Angulo","year":"2014","unstructured":"Garc\u00eda-Angulo VA, Kalita A, Kalita M et al (2014) Comparative genomics and immunoinformatics approach for the identification of vaccine candidates for enterohemorrhagic Escherichia coli O157:H7. Infect Immun 82:2016\u20132026. https:\/\/doi.org\/10.1128\/IAI.01437-13","journal-title":"Infect Immun"},{"key":"559_CR45","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.micpath.2008.01.005","volume":"45","author":"S Babiuk","year":"2008","unstructured":"Babiuk S, Asper DJ, Rogan D et al (2008) Subcutaneous and intranasal immunization with type III secreted proteins can prevent colonization and shedding of Escherichia coli O157:H7 in mice. Microb Pathog 45:7\u201311. https:\/\/doi.org\/10.1016\/j.micpath.2008.01.005","journal-title":"Microb Pathog"},{"key":"559_CR46","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1016\/j.immuni.2004.05.007","volume":"20","author":"M Yoshida","year":"2004","unstructured":"Yoshida M, Claypool SM, Wagner JS et al (2004) Human neonatal Fc receptor mediates transport of IgG into luminal secretions for delivery of antigens to mucosal dendritic cells. Immunity 20:769\u2013783. https:\/\/doi.org\/10.1016\/j.immuni.2004.05.007","journal-title":"Immunity"},{"key":"559_CR47","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1007\/s11033-011-0824-0","volume":"39","author":"H Fan","year":"2012","unstructured":"Fan H, Wang L, Luo J, Long B (2012) Protection against Escherichia coli O157:H7 challenge by immunization of mice with purified Tir proteins. Mol Biol Rep 39:989\u2013997. https:\/\/doi.org\/10.1007\/s11033-011-0824-0","journal-title":"Mol Biol Rep"},{"key":"559_CR48","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1038\/mi.2011.41","volume":"4","author":"NJ Mantis","year":"2011","unstructured":"Mantis NJ, Rol N, Corth\u00e9sy B (2011) Secretory IgA\u2019s complex roles in immunity and mucosal homeostasis in the gut. Mucosal Immunol 4:603\u2013611. https:\/\/doi.org\/10.1038\/mi.2011.41","journal-title":"Mucosal Immunol"},{"key":"559_CR49","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1089\/fpd.2012.1401","volume":"10","author":"LH Gould","year":"2013","unstructured":"Gould LH, Mody RK, Ong KL et al (2013) Increased recognition of non-O157 shiga toxin\u2013producing Escherichia coli infections in the United States during 2000\u20132010: epidemiologic features and comparison with E. coli O157 Infections. Foodborne Pathog Dis 10:453\u2013460. https:\/\/doi.org\/10.1089\/fpd.2012.1401","journal-title":"Foodborne Pathog Dis"},{"key":"559_CR50","doi-asserted-by":"publisher","first-page":"1422","DOI":"10.1086\/466536","volume":"192","author":"JT Brooks","year":"2005","unstructured":"Brooks JT, Sowers EG, Wells JG et al (2005) Non-O157 shiga toxin\u2013producing Escherichia coli infections in the United States, 1983\u20132002. J Infect Dis 192:1422\u20131429. https:\/\/doi.org\/10.1086\/466536","journal-title":"J Infect Dis"},{"key":"559_CR51","doi-asserted-by":"publisher","first-page":"e0128391","DOI":"10.1371\/journal.pone.0128391","volume":"10","author":"TN McNeilly","year":"2015","unstructured":"McNeilly TN, Mitchell MC, Corbishley A et al (2015) Optimizing the protection of cattle against Escherichia coli O157:H7 colonization through immunization with different combinations of H7 Flagellin, Tir, Intimin-531 or EspA. PLoS One 10:e0128391. https:\/\/doi.org\/10.1371\/journal.pone.0128391","journal-title":"PLoS One"}],"container-title":["Medical Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00430-018-0559-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00430-018-0559-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00430-018-0559-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T22:14:34Z","timestamp":1744150474000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00430-018-0559-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,12]]},"references-count":51,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,2,4]]}},"alternative-id":["559"],"URL":"https:\/\/doi.org\/10.1007\/s00430-018-0559-9","relation":{},"ISSN":["0300-8584","1432-1831"],"issn-type":[{"type":"print","value":"0300-8584"},{"type":"electronic","value":"1432-1831"}],"subject":[],"published":{"date-parts":[[2018,9,12]]},"assertion":[{"value":"27 April 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 September 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that no conflict of interest exists.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"For this type of study, formal consent is not required.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"All procedures performed in studies involving animals were in accordance with the ethical standards of the institution as per the Institutional Animal Ethical Committee (IAEC code 15\/2016), DFRL, Mysuru, Karnataka, India.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Statement on the welfare of animals"}},{"value":"The datasets generated during and\/or analysed during the current study are available from the corresponding author on reasonable request.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Data availability statement"}}]}}