{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:13:18Z","timestamp":1792782798176,"version":"4.5.0"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Collaborative Research Center \/ Transregio 124"],"award-info":[{"award-number":["Collaborative Research Center \/ Transregio 124"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Project A2"],"award-info":[{"award-number":["Project A2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Collaborative Research Center \/ Transregio 124"],"award-info":[{"award-number":["Collaborative Research Center \/ Transregio 124"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Project A2"],"award-info":[{"award-number":["Project A2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"IZKF Wuerzburg","award":["Z-3\/56"],"award-info":[{"award-number":["Z-3\/56"]}]},{"DOI":"10.13039\/501100006463","name":"Bayerisches Staatsministerium\u00a0f\u00fcr Wirtschaft und Medien,\u00a0Energie und Technologie","doi-asserted-by":"publisher","award":["BayBIO-1606-003"],"award-info":[{"award-number":["BayBIO-1606-003"]}],"id":[{"id":"10.13039\/501100006463","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Med Microbiol Immunol"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1007\/s00430-020-00665-3","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T08:02:42Z","timestamp":1585641762000},"page":"579-592","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Impact of immunosuppressive and antifungal drugs on PBMC- and whole blood-based flow cytometric CD154+ Aspergillus fumigatus specific T-cell quantification"],"prefix":"10.1007","volume":"209","author":[{"given":"Lukas","family":"Page","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris D.","family":"Lauruschkat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Helm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Weis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Lazariotou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Einsele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Ullmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Loeffler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7784-7256","authenticated-orcid":false,"given":"Sebastian","family":"Wurster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,31]]},"reference":[{"key":"665_CR1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1128\/CMR.00014-09","volume":"22","author":"SJ Park","year":"2009","unstructured":"Park SJ, Mehrad B (2009) Innate immunity to Aspergillus species. Clin Microbiol Rev 22:535\u2013551","journal-title":"Clin Microbiol Rev"},{"key":"665_CR2","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1182\/blood-2003-02-0456","volume":"102","author":"T Fukuda","year":"2003","unstructured":"Fukuda T, Boeckh M, Carter RA, Sandmaier BM, Maris MB, Maloney DG, Martin PJ, Storb RF, Marr KA (2003) Risks and outcomes of invasive fungal infections in recipients of allogeneic hematopoietic stem cell transplants after nonmyeloablative conditioning. Blood 102:827\u2013833","journal-title":"Blood"},{"key":"665_CR3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1086\/590004","volume":"47","author":"G Chamilos","year":"2008","unstructured":"Chamilos G, Lewis RE, Kontoyiannis DP (2008) Delaying amphotericin B-based frontline therapy significantly increases mortality among patients with hematologic malignancy who have zygomycosis. Clin Infect Dis 47:503\u2013509","journal-title":"Clin Infect Dis"},{"issue":"Suppl 3","key":"665_CR4","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/1469-0691.12371","volume":"20","author":"OA Cornely","year":"2014","unstructured":"Cornely OA, Arikan-Akdagli S, Dannaoui E, Groll AH, Lagrou K, Chakrabarti A, Lanternier F, Pagano L, Skiada A, Akova M, Arendrup MC, Boekhout T, Chowdhary A, Cuenca-Estrella M, Freiberger T, Guinea J, Guarro J, de Hoog S, Hope W, Johnson E, Kathuria S, Lackner M, Lass-Florl C, Lortholary O, Meis JF, Meletiadis J, Munoz P, Richardson M, Roilides E, Tortorano AM, Ullmann AJ, van Diepeningen A, Verweij P, Petrikkos G, European Society of Clinical M, Infectious Diseases Fungal Infection Study G (2014) ESCMID and ECMM joint clinical guidelines for the diagnosis and management of mucormycosis. Clin Microbiol Infect 20(Suppl 3):5\u201326","journal-title":"Clin Microbiol Infect"},{"key":"665_CR5","doi-asserted-by":"crossref","first-page":"429","DOI":"10.2217\/bmm.13.129","volume":"8","author":"G Johnson","year":"2014","unstructured":"Johnson G, Ferrini A, Dolan SK, Nolan T, Agrawal S, Doyle S, Bustin SA (2014) Biomarkers for invasive aspergillosis: the challenges continue. Biomark Med 8:429\u2013451","journal-title":"Biomark Med"},{"key":"665_CR6","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1164\/rccm.201407-1235LE","volume":"191","author":"P Bacher","year":"2015","unstructured":"Bacher P, Steinbach A, Kniemeyer O, Hamprecht A, Assenmacher M, Vehreschild MJ, Vehreschild JJ, Brakhage AA, Cornely OA, Scheffold A (2015) Fungus-specific CD4(+) T cells for rapid identification of invasive pulmonary mold infection. Am J Respir Crit Care Med 191:348\u2013352","journal-title":"Am J Respir Crit Care Med"},{"key":"665_CR7","doi-asserted-by":"crossref","first-page":"e74326","DOI":"10.1371\/journal.pone.0074326","volume":"8","author":"L Potenza","year":"2013","unstructured":"Potenza L, Vallerini D, Barozzi P, Riva G, Forghieri F, Beauvais A, Beau R, Candoni A, Maertens J, Rossi G, Morselli M, Zanetti E, Quadrelli C, Codeluppi M, Guaraldi G, Pagano L, Caira M, Del Giovane C, Maccaferri M, Stefani A, Morandi U, Tazzioli G, Girardis M, Delia M, Specchia G, Longo G, Marasca R, Narni F, Merli F, Imovilli A, Apolone G, Carvalho A, Comoli P, Romani L, Latge JP, Luppi M (2013) Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients. PLoS ONE 8:e74326","journal-title":"PLoS ONE"},{"key":"665_CR8","doi-asserted-by":"crossref","first-page":"e0149108","DOI":"10.1371\/journal.pone.0149108","volume":"11","author":"L Potenza","year":"2016","unstructured":"Potenza L, Vallerini D, Barozzi P, Riva G, Gilioli A, Forghieri F, Candoni A, Cesaro S, Quadrelli C, Maertens J, Rossi G, Morselli M, Codeluppi M, Mussini C, Colaci E, Messerotti A, Paolini A, Maccaferri M, Fantuzzi V, Del Giovane C, Stefani A, Morandi U, Maffei R, Marasca R, Narni F, Fanin R, Comoli P, Romani L, Beauvais A, Viale PL, Latge JP, Lewis RE, Luppi M (2016) Mucorales-specific T cells in patients with hematologic malignancies. PLoS ONE 11:e0149108","journal-title":"PLoS ONE"},{"key":"665_CR9","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.3389\/fmicb.2018.01381","volume":"9","author":"FC Koehler","year":"2018","unstructured":"Koehler FC, Cornely OA, Wisplinghoff H, Schauss AC, Salmanton-Garcia J, Ostermann H, Ziegler M, Bacher P, Scheffold A, Alex R, Richter A, Koehler P (2018) Candida-reactive T cells for the diagnosis of invasive candida infection-A prospective pilot study. Front Microbiol. 9:1381","journal-title":"Front Microbiol."},{"issue":"7","key":"665_CR10","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1111\/myc.12919","volume":"62","author":"A Steinbach","year":"2019","unstructured":"Steinbach A, Cornely OA, Wisplinghoff H, Schauss AC, Vehreschild JJ, Rybniker J, Hamprecht A, Richter A, Bacher P, Scheffold A, Koehler P (2019) Mould-reactive T cells for the diagnosis of invasive mould infection-A prospective study. Mycoses 62(7):562\u2013569","journal-title":"Mycoses"},{"key":"665_CR11","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1093\/mmy\/myw054","volume":"55","author":"S Wurster","year":"2017","unstructured":"Wurster S, Weis P, Page L, Lazariotou M, Einsele H, Ullmann AJ (2017) Quantification of A. fumigatus-specific CD154+T-cells-preanalytic considerations. Med Mycol 55:223\u2013227","journal-title":"Med Mycol"},{"key":"665_CR12","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jim.2010.05.005","volume":"359","author":"G Afonso","year":"2010","unstructured":"Afonso G, Scotto M, Renand A, Arvastsson J, Vassilieff D, Cilio CM, Mallone R (2010) Critical parameters in blood processing for T-cell assays: validation on ELISpot and tetramer platforms. J Immunol Methods 359:28\u201336","journal-title":"J Immunol Methods"},{"key":"665_CR13","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.1365-2249.2010.04272.x","volume":"163","author":"R Mallone","year":"2011","unstructured":"Mallone R, Mannering SI, Brooks-Worrell BM, Durinovic-Bello I, Cilio CM, Wong FS, Schloot NC, T-Cell Workshop Committee IoDS (2011) Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell workshop committee of the immunology of diabetes society. Clin Exp Immunol 163:33\u201349","journal-title":"Clin Exp Immunol"},{"issue":"8","key":"665_CR14","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1111\/myc.12780","volume":"61","author":"CD Lauruschkat","year":"2018","unstructured":"Lauruschkat CD, Wurster S, Page L, Lazariotou M, Dragan M, Weis P, Ullmann AJ, Einsele H, L\u00f6ffler J (2018) Susceptibility of A. fumigatus-specific T-cell assays to pre-analytic blood storage and PBMC cryopreservation greatly depends on readout platform and analytes. Mycoses 61(8):549\u2013560","journal-title":"Mycoses"},{"issue":"2","key":"665_CR15","first-page":"187","volume":"58","author":"P Weis","year":"2020","unstructured":"Weis P, Helm J, Page L, Lauruschkat CD, Lazariotou M, Einsele H, Loeffler J, Ullmann AJ, Wurster S (2020) Development and evaluation of a whole blood-based approach for flow cytometric quantification of CD154+mould-reactive T cells. Med Mycol 58(2):187\u2013196","journal-title":"Med Mycol"},{"key":"665_CR16","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1183\/09031936.00013409","volume":"34","author":"U Sester","year":"2009","unstructured":"Sester U, Wilkens H, van Bentum K, Singh M, Sybrecht GW, Schafers HJ, Sester M (2009) Impaired detection of Mycobacterium tuberculosis immunity in patients using high levels of immunosuppressive drugs. Eur Respir J 34:702\u2013710","journal-title":"Eur Respir J"},{"key":"665_CR17","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.crohns.2011.09.011","volume":"6","author":"U Helwig","year":"2012","unstructured":"Helwig U, Muller M, Hedderich J, Schreiber S (2012) Corticosteroids and immunosuppressive therapy influence the result of QuantiFERON TB Gold testing in inflammatory bowel disease patients. J Crohns Colitis 6:419\u2013424","journal-title":"J Crohns Colitis"},{"key":"665_CR18","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ijmyco.2016.02.005","volume":"5","author":"EN Ndzi","year":"2016","unstructured":"Ndzi EN, Nkenfou CN, Gwom LC, Fainguem N, Fokam J, Pefura Y (2016) The pros and cons of the QuantiFERON test for the diagnosis of tuberculosis, prediction of disease progression, and treatment monitoring. Int J Mycobacteriol 5:177\u2013184","journal-title":"Int J Mycobacteriol"},{"key":"665_CR19","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1177\/1753425914567692","volume":"21","author":"JF Hedges","year":"2015","unstructured":"Hedges JF, Mitchell AM, Jones K, Kimmel E, Ramstead AG, Snyder DT, Jutila MA (2015) Amphotericin B stimulates gammadelta T and NK cells, and enhances protection from Salmonella infection. Innate Immun 21:598\u2013608","journal-title":"Innate Immun"},{"key":"665_CR20","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1086\/314495","volume":"178","author":"PD Rogers","year":"1998","unstructured":"Rogers PD, Jenkins JK, Chapman SW, Ndebele K, Chapman BA, Cleary JD (1998) Amphotericin B activation of human genes encoding for cytokines. J Infect Dis 178:1726\u20131733","journal-title":"J Infect Dis"},{"key":"665_CR21","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0952-3278(98)90148-X","volume":"58","author":"K Shindo","year":"1998","unstructured":"Shindo K, Fukumura M, Ito A (1998) Inhibitory effect of amphotericin B on leukotriene B4 synthesis in human neutrophils in vitro. Prostaglandins Leukot Essent Fatty Acids 58:105\u2013109","journal-title":"Prostaglandins Leukot Essent Fatty Acids"},{"key":"665_CR22","doi-asserted-by":"crossref","first-page":"37561","DOI":"10.1074\/jbc.M306137200","volume":"278","author":"K Sau","year":"2003","unstructured":"Sau K, Mambula SS, Latz E, Henneke P, Golenbock DT, Levitz SM (2003) The antifungal drug amphotericin B promotes inflammatory cytokine release by a Toll-like receptor- and CD14-dependent mechanism. J Biol Chem 278:37561\u201337568","journal-title":"J Biol Chem"},{"key":"665_CR23","doi-asserted-by":"crossref","first-page":"704","DOI":"10.3109\/13693780903471784","volume":"48","author":"JH Choi","year":"2010","unstructured":"Choi JH, Kwon EY, Park CM, Choi SM, Lee DG, Yoo JH, Shin WS, Stevens DA (2010) Immunomodulatory effects of antifungal agents on the response of human monocytic cells to Aspergillus fumigatus conidia. Med Mycol 48:704\u2013709","journal-title":"Med Mycol"},{"key":"665_CR24","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1128\/AAC.35.5.879","volume":"35","author":"JM Boggs","year":"1991","unstructured":"Boggs JM, Chang NH, Goundalkar A (1991) Liposomal amphotericin B inhibits in vitro T-lymphocyte response to antigen. Antimicrob Agents Chemother 35:879\u2013885","journal-title":"Antimicrob Agents Chemother"},{"key":"665_CR25","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1086\/315517","volume":"181","author":"E Reyes","year":"2000","unstructured":"Reyes E, Cardona J, Prieto A, Bernstein ED, Rodriguez-Zapata M, Pontes MJ, Alvarez-Mon M (2000) Liposomal amphotericin B and amphotericin B-deoxycholate show different immunoregulatory effects on human peripheral blood mononuclear cells. J Infect Dis 181:2003\u20132010","journal-title":"J Infect Dis"},{"key":"665_CR26","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1097\/MAJ.0000000000000236","volume":"348","author":"I Fidan","year":"2014","unstructured":"Fidan I, Yesilyurt E, Kalkanci A, Aslan SO, Sahin N, Ogan MC, Dizbay M (2014) Immunomodulatory effects of voriconazole and caspofungin on human peripheral blood mononuclear cells stimulated by Candida albicans and Candida krusei. Am J Med Sci 348:219\u2013223","journal-title":"Am J Med Sci"},{"key":"665_CR27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.immuni.2012.07.010","volume":"37","author":"CM Britten","year":"2012","unstructured":"Britten CM, Janetzki S, Butterfield LH, Ferrari G, Gouttefangeas C, Huber C, Kalos M, Levitsky HI, Maecker HT, Melief CJ, O\u2019Donnell-Tormey J, Odunsi K, Old LJ, Ottenhoff TH, Ottensmeier C, Pawelec G, Roederer M, Roep BO, Romero P, van der Burg SH, Walter S, Hoos A, Davis MM (2012) T cell assays and MIATA: the essential minimum for maximum impact. Immunity 37:1\u20132","journal-title":"Immunity"},{"issue":"4","key":"665_CR28","doi-asserted-by":"crossref","first-page":"E47","DOI":"10.3390\/cells6040047","volume":"6","author":"A Schiller","year":"2017","unstructured":"Schiller A, Zhang T, Li R, Duechting A, Sundararaman S, Przybyla A, Kuerten S, Lehmann PV (2017) A positive control for detection of functional CD4 T cells in PBMC: the CPI pool. Cells 6(4):E47","journal-title":"Cells"},{"key":"665_CR29","doi-asserted-by":"crossref","first-page":"2340","DOI":"10.1002\/ibd.21605","volume":"17","author":"E Belard","year":"2011","unstructured":"Belard E, Semb S, Ruhwald M, Werlinrud AM, Soborg B, Jensen FK, Thomsen H, Brylov A, Hetland ML, Nordgaard-Lassen I, Ravn P (2011) Prednisolone treatment affects the performance of the QuantiFERON gold in-tube test and the tuberculin skin test in patients with autoimmune disorders screened for latent tuberculosis infection. Inflamm Bowel Dis 17:2340\u20132349","journal-title":"Inflamm Bowel Dis"},{"key":"665_CR30","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1183\/09031936.00194314","volume":"45","author":"RJ Hewitt","year":"2015","unstructured":"Hewitt RJ, Singanayagam A, Sridhar S, Wickremasinghe M, Min Kon O (2015) Screening for latent tuberculosis before tumour necrosis factor antagonist therapy. Eur Respir J 45:1510\u20131512","journal-title":"Eur Respir J"},{"key":"665_CR31","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1128\/IAI.01700-14","volume":"82","author":"L Tramsen","year":"2014","unstructured":"Tramsen L, Schmidt S, Roeger F, Schubert R, Salzmann-Manrique E, Latge JP, Klingebiel T, Lehrnbecher T (2014) Immunosuppressive compounds exhibit particular effects on functional properties of human anti-Aspergillus Th1 cells. Infect Immun 82:2649\u20132656","journal-title":"Infect Immun"},{"key":"665_CR32","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1172\/JCI117089","volume":"93","author":"R Fuleihan","year":"1994","unstructured":"Fuleihan R, Ramesh N, Horner A, Ahern D, Belshaw PJ, Alberg DG, Stamenkovic I, Harmon W, Geha RS (1994) Cyclosporin A inhibits CD40 ligand expression in T lymphocytes. J Clin Invest 93:1315\u20131320","journal-title":"J Clin Invest"},{"key":"665_CR33","doi-asserted-by":"crossref","first-page":"29624","DOI":"10.1074\/jbc.270.50.29624","volume":"270","author":"LA Schubert","year":"1995","unstructured":"Schubert LA, King G, Cron RQ, Lewis DB, Aruffo A, Hollenbaugh D (1995) The human gp39 promoter. Two distinct nuclear factors of activated T cell protein-binding elements contribute independently to transcriptional activation. J Biol Chem 270:29624\u201329627","journal-title":"J Biol Chem"},{"key":"665_CR34","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1172\/JCI10168","volume":"107","author":"HH Jabara","year":"2001","unstructured":"Jabara HH, Brodeur SR, Geha RS (2001) Glucocorticoids upregulate CD40 ligand expression and induce CD40L-dependent immunoglobulin isotype switching. J Clin Invest 107:371\u2013378","journal-title":"J Clin Invest"},{"key":"665_CR35","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1006\/cimm.1998.1308","volume":"187","author":"F Bischof","year":"1998","unstructured":"Bischof F, Melms A (1998) Glucocorticoids inhibit CD40 ligand expression of peripheral CD4+lymphocytes. Cell Immunol 187:38\u201344","journal-title":"Cell Immunol"},{"key":"665_CR36","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/B978-0-12-800885-0.00002-3","volume-title":"Personalized Immunosuppression in Transplantation","author":"A Dasgupta","year":"2016","unstructured":"Dasgupta A (2016) Limitations of immunoassays used for therapeutic drug monitoring of immunosuppressants. In: Oellerich M, Dasgupta A (eds) Personalized Immunosuppression in Transplantation. Elsevier, Amsterdam, pp 29\u201356"},{"key":"665_CR37","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.imlet.2011.06.010","volume":"140","author":"J Leitner","year":"2011","unstructured":"Leitner J, Drobits K, Pickl WF, Majdic O, Zlabinger G, Steinberger P (2011) The effects of Cyclosporine A and azathioprine on human T cells activated by different costimulatory signals. Immunol Lett 140:74\u201380","journal-title":"Immunol Lett"},{"key":"665_CR38","doi-asserted-by":"crossref","first-page":"7700","DOI":"10.1074\/jbc.271.13.7700","volume":"271","author":"P Ghosh","year":"1996","unstructured":"Ghosh P, Sica A, Cippitelli M, Subleski J, Lahesmaa R, Young HA, Rice NR (1996) Activation of nuclear factor of activated T cells in a cyclosporin A-resistant pathway. J Biol Chem 271:7700\u20137704","journal-title":"J Biol Chem"},{"issue":"4","key":"665_CR39","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.ymeth.2005.12.004","volume":"38","author":"MC Gauduin","year":"2006","unstructured":"Gauduin MC (2006) Intracellular cytokine staining for the characterization and quantitation of antigen-specific T lymphocyte responses. Methods 38(4):263\u2013273","journal-title":"Methods"},{"key":"665_CR40","doi-asserted-by":"crossref","first-page":"5284","DOI":"10.4049\/jimmunol.161.10.5284","volume":"161","author":"SL Waldrop","year":"1998","unstructured":"Waldrop SL, Davis KA, Maino VC, Picker LJ (1998) Normal human CD4+memory T cells display broad heterogeneity in their activation threshold for cytokine synthesis. J Immunol 161:5284\u20135295","journal-title":"J Immunol"},{"key":"665_CR41","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jim.2016.04.010","volume":"434","author":"J Lemieux","year":"2016","unstructured":"Lemieux J, Jobin C, Simard C, Neron S (2016) A global look into human T cell subsets before and after cryopreservation using multiparametric flow cytometry and two-dimensional visualization analysis. J Immunol Methods 434:73\u201382","journal-title":"J Immunol Methods"},{"issue":"4","key":"665_CR42","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/j.cell.2016.09.050","volume":"167","author":"P Bacher","year":"2016","unstructured":"Bacher P, Heinrich F, Stervbo U, Nienen M, Vahldieck M, Iwert C, Vogt K, Kollet J, Babel N, Sawitzki B, Schwarz C, Bereswill S, Heimesaat MM, Heine G, Gadermaier G, Asam C, Assenmacher M, Kniemeyer O, Brakhage AA, Ferreira F, Wallner M, Worm M, Scheffold A (2016) Regulatory T cell specificity directs tolerance versus allergy against aeroantigens in humans. Cell 167(4):1067\u20131078","journal-title":"Cell"},{"issue":"7","key":"665_CR43","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.4049\/jimmunol.1400776","volume":"193","author":"P Bacher","year":"2014","unstructured":"Bacher P, Kniemeyer O, Teutschbein J, Th\u00f6n M, V\u00f6disch M, Wartenberg D, Scharf DH, Koester-Eiserfunke N, Sch\u00fctte M, D\u00fcbel S, Assenmacher M, Brakhage AA, Scheffold A (2014) Identification of immunogenic antigens from Aspergillus fumigatus by direct multiparameter characterization of specific conventional and regulatory CD4+\u2009 cells. J Immunol. 193(7):3332\u20133343","journal-title":"J Immunol."},{"issue":"8","key":"665_CR44","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1016\/j.ijmm.2018.09.002","volume":"308","author":"L Page","year":"2018","unstructured":"Page L, Weis P, M\u00fcller T, Dittrich M, Lazariotou M, Dragan M, Waaga-Gasser AM, Helm J, Dandekar T, Einsele H, L\u00f6ffler J, Ullmann AJ, Wurster S (2018) Evaluation of Aspergillus and Mucorales specific T-cells and peripheral blood mononuclear cell cytokine signatures as biomarkers of environmental mold exposure. Int J Med Microbiol 308(8):1018\u20131026","journal-title":"Int J Med Microbiol"},{"key":"665_CR45","doi-asserted-by":"crossref","first-page":"429","DOI":"10.3389\/fmicb.2015.00429","volume":"6","author":"R Thakur","year":"2015","unstructured":"Thakur R, Anand R, Tiwari S, Singh AP, Tiwary BN, Shankar J (2015) Cytokines induce effector T-helper cells during invasive aspergillosis; what we have learned about T-helper cells? Front Microbiol. 6:429","journal-title":"Front Microbiol."},{"key":"665_CR46","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1128\/AAC.47.6.1818-1823.2003","volume":"47","author":"HL Van Epps","year":"2003","unstructured":"Van Epps HL, Feldmesser M, Pamer EG (2003) Voriconazole inhibits fungal growth without impairing antigen presentation or T-cell activation. Antimicrob Agents Chemother 47:1818\u20131823","journal-title":"Antimicrob Agents Chemother"},{"key":"665_CR47","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1111\/tid.12089","volume":"15","author":"L Tramsen","year":"2013","unstructured":"Tramsen L, Schmidt S, Koehl U, Huenecke S, Latge JP, Roeger F, Schubert R, Klingebiel T, Lehrnbecher T (2013) No effect of antifungal compounds on functional properties of human antifungal T-helper type 1 cells. Transpl Infect Dis 15:430\u2013434","journal-title":"Transpl Infect Dis"},{"key":"665_CR48","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1086\/429921","volume":"40","author":"KA Marr","year":"2005","unstructured":"Marr KA, Laverdiere M, Gugel A, Leisenring W (2005) Antifungal therapy decreases sensitivity of the Aspergillus galactomannan enzyme immunoassay. Clin Infect Dis 40:1762\u20131769","journal-title":"Clin Infect Dis"},{"key":"665_CR49","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.1093\/jac\/dks208","volume":"67","author":"M Reinwald","year":"2012","unstructured":"Reinwald M, Hummel M, Kovalevskaya E, Spiess B, Heinz WJ, Vehreschild JJ, Schultheis B, Krause SW, Claus B, Suedhoff T, Schwerdtfeger R, Reuter S, Kiehl MG, Hofmann WK, Buchheidt D (2012) Therapy with antifungals decreases the diagnostic performance of PCR for diagnosing invasive aspergillosis in bronchoalveolar lavage samples of patients with haematological malignancies. J Antimicrob Chemother 67:2260\u20132267","journal-title":"J Antimicrob Chemother"},{"key":"665_CR50","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/jac\/dkh542","volume":"55","author":"S Bellocchio","year":"2005","unstructured":"Bellocchio S, Gaziano R, Bozza S, Rossi G, Montagnoli C, Perruccio K, Calvitti M, Pitzurra L, Romani L (2005) Liposomal amphotericin B activates antifungal resistance with reduced toxicity by diverting Toll-like receptor ignaling from TLR-2 to TLR-4. J Antimicrob Chemother 55:214\u2013222","journal-title":"J Antimicrob Chemother"},{"key":"665_CR51","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1086\/589304","volume":"198","author":"TM Hohl","year":"2008","unstructured":"Hohl TM, Feldmesser M, Perlin DS, Pamer EG (2008) Caspofungin modulates inflammatory responses to Aspergillus fumigatus through stage-specific effects on fungal beta-glucan exposure. J Infect Dis 198:176\u2013185","journal-title":"J Infect Dis"},{"key":"665_CR52","doi-asserted-by":"crossref","first-page":"3301","DOI":"10.1128\/AAC.01018-07","volume":"52","author":"M Simitsopoulou","year":"2008","unstructured":"Simitsopoulou M, Roilides E, Paliogianni F, Likartsis C, Ioannidis J, Kanellou K, Walsh TJ (2008) Immunomodulatory effects of voriconazole on monocytes challenged with Aspergillus fumigatus: differential role of Toll-like receptors. Antimicrob Agents Chemother 52:3301\u20133306","journal-title":"Antimicrob Agents Chemother"},{"key":"665_CR53","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1111\/j.1574-695X.2010.00720.x","volume":"60","author":"S Salvenmoser","year":"2010","unstructured":"Salvenmoser S, Seidler MJ, Dalpke A, Muller FM (2010) Effects of caspofungin, Candida albicans and Aspergillus fumigatus on toll-like receptor 9 of GM-CSF-stimulated PMNs. FEMS Immunol Med Microbiol 60:74\u201377","journal-title":"FEMS Immunol Med Microbiol"},{"key":"665_CR54","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1097\/QCO.0b013e328348b159","volume":"24","author":"RA Cramer","year":"2011","unstructured":"Cramer RA, Rivera A, Hohl TM (2011) Immune responses against Aspergillus fumigatus: what have we learned? Curr Opin Infect Dis 24:315\u2013322","journal-title":"Curr Opin Infect Dis"},{"key":"665_CR55","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0378-5173(99)00200-8","volume":"188","author":"S Saxena","year":"1999","unstructured":"Saxena S, Bhatnagar PK, Ghosh PC, Sarma PU (1999) Effect of amphotericin B lipid formulation on immune response in aspergillosis. Int J Pharm 188:19\u201330","journal-title":"Int J Pharm"},{"key":"665_CR56","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1093\/jac\/dkg367","volume":"52","author":"MJ Becker","year":"2003","unstructured":"Becker MJ, de Marie S, Fens MH, Verbrugh HA, Bakker-Woudenberg IA (2003) Effect of amphotericin B treatment on kinetics of cytokines and parameters of fungal load in neutropenic rats with invasive pulmonary aspergillosis. J Antimicrob Chemother 52:428\u2013434","journal-title":"J Antimicrob Chemother"}],"container-title":["Medical Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00430-020-00665-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00430-020-00665-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00430-020-00665-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T05:40:10Z","timestamp":1695966010000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00430-020-00665-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,31]]},"references-count":56,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["665"],"URL":"https:\/\/doi.org\/10.1007\/s00430-020-00665-3","relation":{},"ISSN":["0300-8584","1432-1831"],"issn-type":[{"value":"0300-8584","type":"print"},{"value":"1432-1831","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,31]]},"assertion":[{"value":"25 September 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 March 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 March 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors have no conflict of interest related to this study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Informed consent, approved by the ethics committee of the University of Wuerzburg (105\/15 and 42\/17), was obtained from all blood donors.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}