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Besides nucleic acid-based test systems and point-of-care (POCT) antigen (Ag) tests, quantitative, laboratory-based nucleocapsid Ag tests for SARS-CoV-2 have recently been launched. Here, we evaluated four commercial Ag tests on automated platforms and one POCT to detect SARS-CoV-2. We evaluated PCR-positive (<jats:italic>n<\/jats:italic>\u2009=\u2009107) and PCR-negative (<jats:italic>n<\/jats:italic>\u2009=\u2009303) respiratory swabs from asymptomatic and symptomatic patients at the end of the second pandemic wave in Germany (February\u2013March 2021) as well as clinical isolates EU1 (B.1.117), variant of concern (VOC) Alpha (B.1.1.7) or Beta (B.1.351), which had been expanded in a biosafety level 3 laboratory. The specificities of automated SARS-CoV-2 Ag tests ranged between 97.0 and 99.7% (Lumipulse G SARS-CoV-2 Ag (Fujirebio): 97.03%, Elecsys SARS-CoV-2 Ag (Roche Diagnostics): 97.69%; LIAISON<jats:sup>\u00ae<\/jats:sup> SARS-CoV-2 Ag (Diasorin) and SARS-CoV-2 Ag ELISA (Euroimmun): 99.67%). In this study cohort of hospitalized patients, the clinical sensitivities of tests were low, ranging from 17.76 to 52.34%, and analytical sensitivities ranged from 420,000 to 25,000,000 Geq\/ml. In comparison, the detection limit of the Roche Rapid Ag Test (RAT) was 9,300,000 Geq\/ml, detecting 23.58% of respiratory samples. Receiver-operating-characteristics (ROCs) and Youden\u2019s index analyses were performed to further characterize the assays\u2019 overall performance and determine optimal assay cutoffs for sensitivity and specificity. VOCs carrying up to four amino acid mutations in nucleocapsid were detected by all five assays with characteristics comparable to non-VOCs. In summary, automated, quantitative SARS-CoV-2 Ag tests show variable performance and are not necessarily superior to a standard POCT. The efficacy of any alternative testing strategies to complement nucleic acid-based assays must be carefully evaluated by independent laboratories prior to widespread implementation.<\/jats:p>","DOI":"10.1007\/s00430-021-00719-0","type":"journal-article","created":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T07:03:04Z","timestamp":1629442984000},"page":"263-275","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Comparison of four commercial, automated antigen tests to detect SARS-CoV-2 variants of concern"],"prefix":"10.1007","volume":"210","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5435-5972","authenticated-orcid":false,"given":"Andreas","family":"Osterman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maximilian","family":"Iglhaut","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Lehner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Sp\u00e4th","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7692-6905","authenticated-orcid":false,"given":"Marcel","family":"Stern","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna","family":"Autenrieth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7016-0470","authenticated-orcid":false,"given":"Maximilian","family":"Muenchhoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4435-6861","authenticated-orcid":false,"given":"Alexander","family":"Graf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5112-9507","authenticated-orcid":false,"given":"Stefan","family":"Krebs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3994-2332","authenticated-orcid":false,"given":"Helmut","family":"Blum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armin","family":"Baiker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natascha","family":"Grzimek-Koschewa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9421-1911","authenticated-orcid":false,"given":"Ulrike","family":"Protzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2359-2294","authenticated-orcid":false,"given":"Lars","family":"Kaderali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0589-697X","authenticated-orcid":false,"given":"Hanna-Mari","family":"Baldauf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1384-8946","authenticated-orcid":false,"given":"Oliver T.","family":"Keppler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,8,20]]},"reference":[{"issue":"7","key":"719_CR1","doi-asserted-by":"publisher","first-page":"e311","DOI":"10.1016\/s2666-5247(21)00056-2","volume":"2","author":"VM Corman","year":"2021","unstructured":"Corman VM, Haage VC, Bleicker T, Schmidt ML, M\u00fchlemann B, Zuchowski M, Jo WK, Tscheak P, M\u00f6ncke-Buchner E, M\u00fcller MA, Krumbholz A, Drexler JF, Drosten C (2021) Comparison of seven commercial SARS-CoV-2 rapid point-of-care antigen tests: a single-centre laboratory evaluation study. 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