{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T09:19:03Z","timestamp":1787476743693,"version":"build-2736575974"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universit\u00e4tsklinikum Hamburg-Eppendorf (UKE)"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Med Microbiol Immunol"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    With around 2\u00a0billion cases each year, infectious gastroenteritis remains a worldwide health problem. A major cause of acute gastroenteritis is infection with enteric viruses, which often leads to hospitalization in children and immunocompromised people. We adapted and validated a gastrointestinal qPCR panel, which simultaneously detects the most common enteric viruses: Norovirus GI and GII, Rotavirus, Adenovirus, Sapovirus, Astrovirus and Enterovirus in stool samples on a fully automated, high-throughput system (Roche cobas5800\/6800\/8800). Limits of detection (LOD), linear range and precision were determined using dilutions of clinical stool samples, which were quantified by digital droplet PCR. Specificity and sensitivity were evaluated using clinical stool samples from patients with diarrhoea and results were compared with commercial CE-IVD qPCR assays. LODs were below 100 for all targets except for Norovirus GI (3,180 copies\/ml), Norovirus GII (299 copies\/ml) and Rotavirus (851 copies\/ml). The assay showed excellent linearity over 5\u20136 log steps for all pathogens (r\n                    <jats:sup>2<\/jats:sup>\n                    : 0.992\u20130.998). For inclusivity External Quality Assessment samples were correctly identified, and no false positives occurred in exclusivity panels containing 26 bacterial isolates and 12 clinical virus samples. Specificity and sensitivity determined by using 243 patient samples ranged between 98.2 and 100.0% and 85.7\u2013100.0%, respectively. In this study we validated a lab-developed syndromic qPCR assay that reliably detects the seven most common enteric viruses in clinical stool samples. Our assay provides a fast, fully automated and easily scalable high-throughput solution for gastrointestinal routine virus testing and screening in high-risk patient groups and outbreaks.\n                  <\/jats:p>","DOI":"10.1007\/s00430-025-00837-z","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T05:15:56Z","timestamp":1748927756000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Adaptation and validation of a gastrointestinal panel to detect diarrheal virus pathogens on a high-throughput qPCR system"],"prefix":"10.1007","volume":"214","author":[{"given":"Katja","family":"Giersch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"N\u00f6rz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz","family":"Grunwald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Pfefferle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa Sophie","family":"Pfl\u00fcger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Aepfelbacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"L\u00fctgehetmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"key":"837_CR1","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/j.cll.2015.02.006","volume":"35","author":"H Zhang","year":"2015","unstructured":"Zhang H, Morrison S, Tang Y-W (2015) Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis. Clin Lab Med 35:461\u2013486. https:\/\/doi.org\/10.1016\/j.cll.2015.02.006","journal-title":"Clin Lab Med"},{"key":"837_CR2","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1016\/S1473-3099(17)30276-1","volume":"17","author":"Estimates of","year":"2017","unstructured":"Estimates of (2017) global, regional, and National morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the global burden of disease study 2015. Lancet Infect Dis 17:909\u2013948. https:\/\/doi.org\/10.1016\/S1473-3099(17)30276-1","journal-title":"Lancet Infect Dis"},{"key":"837_CR3","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1016\/S0140-6736(23)02037-8","volume":"403","author":"TG Flynn","year":"2024","unstructured":"Flynn TG, Olortegui MP, Kosek MN (2024) Viral gastroenteritis. Lancet 403:862\u2013876. https:\/\/doi.org\/10.1016\/S0140-6736(23)02037-8","journal-title":"Lancet"},{"key":"837_CR4","doi-asserted-by":"publisher","first-page":"e01457","DOI":"10.1128\/JCM.01457-17","volume":"56","author":"SG Beal","year":"2018","unstructured":"Beal SG, Tremblay EE, Toffel S, Velez L, Rand KH (2018) A Gastrointestinal PCR panel improves clinical management and lowers health care costs. J Clin Microbiol 56:e01457\u2013e01417. https:\/\/doi.org\/10.1128\/JCM.01457-17","journal-title":"J Clin Microbiol"},{"key":"837_CR5","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/S0140-6736(18)31128-0","volume":"392","author":"K B\u00e1nyai","year":"2018","unstructured":"B\u00e1nyai K, Estes MK, Martella V, Parashar UD (2018) Viral gastroenteritis. Lancet 392:175\u2013186. https:\/\/doi.org\/10.1016\/S0140-6736(18)31128-0","journal-title":"Lancet"},{"key":"837_CR6","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/S1473-3099(11)70253-5","volume":"12","author":"JE Tate","year":"2012","unstructured":"Tate JE, Burton AH, Boschi-Pinto C, Steele AD, Duque J, Parashar UD et al (2012) 2008 Estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis. Lancet Infect Dis 12:136\u2013141. https:\/\/doi.org\/10.1016\/S1473-3099(11)70253-5","journal-title":"Lancet Infect Dis"},{"key":"837_CR7","doi-asserted-by":"publisher","first-page":"e72788","DOI":"10.1371\/journal.pone.0072788","volume":"8","author":"CF Lanata","year":"2013","unstructured":"Lanata CF, Fischer-Walker CL, Olascoaga AC, Torres CX, Aryee MJ, Black RE et al (2013) Global causes of diarrheal disease mortality in children\u2009<\u20095 years of age: a systematic review. PLoS ONE 8:e72788. https:\/\/doi.org\/10.1371\/journal.pone.0072788","journal-title":"PLoS ONE"},{"key":"837_CR8","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.3390\/v12121432","volume":"12","author":"X Fernandez-Cassi","year":"2020","unstructured":"Fernandez-Cassi X, Mart\u00ednez-Puchol S, Silva-Sales M, Cornejo T, Bartolome R, Bofill-Mas S et al (2020) Unveiling viruses associated with gastroenteritis using a metagenomics approach. Viruses 12:1432. https:\/\/doi.org\/10.3390\/v12121432","journal-title":"Viruses"},{"key":"837_CR9","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1016\/S2352-4642(19)30270-6","volume":"3","author":"S Becker-Dreps","year":"2019","unstructured":"Becker-Dreps S, Bucardo F, Vinj\u00e9 J (2019) Sapovirus: an important cause of acute gastroenteritis in children. Lancet Child Adolesc Health 3:758\u2013759. https:\/\/doi.org\/10.1016\/S2352-4642(19)30270-6","journal-title":"Lancet Child Adolesc Health"},{"key":"837_CR10","doi-asserted-by":"publisher","first-page":"001795","DOI":"10.1099\/jmm.0.001795","volume":"73","author":"J Ambrosius-Eichner","year":"2024","unstructured":"Ambrosius-Eichner J, Hogardt M, Berger A, Dultz G, Idris R, Kempf VAJ et al (2024) Comparative evaluation of the detection rate, workflow and associated costs of a multiplex PCR panel versus conventional methods in diagnosis of infectious gastroenteritis. J Med Microbiol 73:001795. https:\/\/doi.org\/10.1099\/jmm.0.001795","journal-title":"J Med Microbiol"},{"key":"837_CR11","doi-asserted-by":"publisher","first-page":"3286","DOI":"10.1111\/jgh.15581","volume":"36","author":"R Teh","year":"2021","unstructured":"Teh R, Tee WD, Tan E, Fan K, Koh CJ, Tambyah PA et al (2021) Review of the role of Gastrointestinal multiplex polymerase chain reaction in the management of diarrheal illness. J Gastroenterol Hepatol 36:3286\u20133297. https:\/\/doi.org\/10.1111\/jgh.15581","journal-title":"J Gastroenterol Hepatol"},{"key":"837_CR12","doi-asserted-by":"publisher","first-page":"3723","DOI":"10.1128\/jcm.02103-15","volume":"53","author":"MJ Binnicker","year":"2015","unstructured":"Binnicker MJ (2015) Multiplex molecular panels for diagnosis of Gastrointestinal infection: performance, result interpretation, and Cost-Effectiveness. J Clin Microbiol 53:3723\u20133728. https:\/\/doi.org\/10.1128\/jcm.02103-15","journal-title":"J Clin Microbiol"},{"key":"837_CR13","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1007\/s10096-023-04739-x","volume":"43","author":"B Massa","year":"2024","unstructured":"Massa B, Vervaeke S, Van Hoecke F (2024) Semi-quantitative assessment of Gastrointestinal viruses in stool samples with seegene allplex Gastrointestinal panel assays: a solution to the interpretation problem of multiple pathogen detection? Eur J Clin Microbiol Infect Dis 43:435\u2013443. https:\/\/doi.org\/10.1007\/s10096-023-04739-x","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"837_CR14","doi-asserted-by":"publisher","unstructured":"Jo SJ, Kang HM, Kim JO, Cho H, Heo W, Yoo IY et al Evaluation of the biofire Gastrointestinal panel to detect diarrheal pathogens in pediatric patients. Diagnostics 2022;12. https:\/\/doi.org\/10.3390\/diagnostics12010034","DOI":"10.3390\/diagnostics12010034"},{"key":"837_CR15","doi-asserted-by":"publisher","first-page":"e01737","DOI":"10.1128\/JCM.01737-19","volume":"58","author":"SA Boers","year":"2020","unstructured":"Boers SA, Peters CJA, Wessels E, Melchers WJG, Claas ECJ (2020) Performance of the QIAstat-Dx Gastrointestinal panel for diagnosing infectious gastroenteritis. J Clin Microbiol 58:e01737\u2013e01719. https:\/\/doi.org\/10.1128\/JCM.01737-19","journal-title":"J Clin Microbiol"},{"key":"837_CR16","doi-asserted-by":"publisher","first-page":"403","DOI":"10.2217\/fmb.15.152","volume":"11","author":"G Greub","year":"2016","unstructured":"Greub G, Sahli R, Brouillet R, Jaton K (2016) Ten years of R&D and full automation in molecular diagnosis. Future Microbiol 11:403\u2013425. https:\/\/doi.org\/10.2217\/fmb.15.152","journal-title":"Future Microbiol"},{"key":"837_CR17","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.1002\/jmv.22162","volume":"83","author":"SA Feeney","year":"2011","unstructured":"Feeney SA, Armstrong VJ, Mitchell SJ, Crawford L, McCaughey C, Coyle PV (2011) Development and clinical validation of multiplex TaqMan\u00ae assays for rapid diagnosis of viral gastroenteritis. J Med Virol 83:1650\u20131656. https:\/\/doi.org\/10.1002\/jmv.22162","journal-title":"J Med Virol"},{"key":"837_CR18","doi-asserted-by":"publisher","unstructured":"Chen D (2014) Development of two multiplex Real-Time PCR assays for the rapid detection of RNA and DNA viruses associated with gastroenteritis in pediatric patients. Pediat Therapeut 04. https:\/\/doi.org\/10.4172\/2161-0665.1000208","DOI":"10.4172\/2161-0665.1000208"},{"key":"837_CR19","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1002\/jmv.21228","volume":"80","author":"MM Freeman","year":"2008","unstructured":"Freeman MM, Kerin T, Hull J, McCaustland K, Gentsch J (2008) Enhancement of detection and quantification of rotavirus in stool using a modified real-time RT-PCR assay. J Med Virol 80:1489\u20131496. https:\/\/doi.org\/10.1002\/jmv.21228","journal-title":"J Med Virol"},{"key":"837_CR20","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1002\/jmv.10382","volume":"70","author":"A Heim","year":"2003","unstructured":"Heim A, Ebnet C, Harste G, Pring-Akerblom P (2003) Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J Med Virol 70:228\u2013239. https:\/\/doi.org\/10.1002\/jmv.10382","journal-title":"J Med Virol"},{"key":"837_CR21","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1128\/JCM.03297-12","volume":"51","author":"X Lu","year":"2013","unstructured":"Lu X, Trujillo-Lopez E, Lott L, Erdman DD (2013) Quantitative real-time PCR assay panel for detection and type-specific identification of epidemic respiratory human adenoviruses. J Clin Microbiol 51:1089\u20131093. https:\/\/doi.org\/10.1128\/JCM.03297-12","journal-title":"J Clin Microbiol"},{"key":"837_CR22","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1002\/jmv.20699","volume":"78","author":"T Oka","year":"2006","unstructured":"Oka T, Katayama K, Hansman GS, Kageyama T, Ogawa S, Wu F-T et al (2006) Detection of human Sapovirus by real-time reverse transcription-polymerase chain reaction. J Med Virol 78:1347\u20131353. https:\/\/doi.org\/10.1002\/jmv.20699","journal-title":"J Med Virol"},{"key":"837_CR23","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.jcv.2010.12.009","volume":"50","author":"J Liu","year":"2011","unstructured":"Liu J, Kibiki G, Maro V, Maro A, Kumburu H, Swai N et al (2011) Multiplex reverse transcription PCR luminex assay for detection and quantitation of viral agents of gastroenteritis. J Clin Virol 50:308\u2013313. https:\/\/doi.org\/10.1016\/j.jcv.2010.12.009","journal-title":"J Clin Virol"},{"key":"837_CR24","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.jcv.2007.11.016","volume":"42","author":"U Dierssen","year":"2008","unstructured":"Dierssen U, Rehren F, Henke-Gendo C, Harste G, Heim A (2008) Rapid routine detection of enterovirus RNA in cerebrospinal fluid by a one-step real-time RT-PCR assay. J Clin Virol 42:58\u201364. https:\/\/doi.org\/10.1016\/j.jcv.2007.11.016","journal-title":"J Clin Virol"},{"key":"837_CR25","doi-asserted-by":"publisher","first-page":"2208","DOI":"10.1128\/JCM.00455-17","volume":"55","author":"JL Cannon","year":"2017","unstructured":"Cannon JL, Barclay L, Collins NR, Wikswo ME, Castro CJ, Maga\u00f1a LC et al (2017) Genetic and epidemiologic trends of Norovirus outbreaks in the united States from 2013 to 2016 demonstrated emergence of novel GII.4 Recombinant viruses. J Clin Microbiol 55:2208\u20132221. https:\/\/doi.org\/10.1128\/JCM.00455-17","journal-title":"J Clin Microbiol"},{"key":"837_CR26","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.jcv.2010.07.019","volume":"49","author":"NM van Maarseveen","year":"2010","unstructured":"van Maarseveen NM, Wessels E, de Brouwer CS, Vossen ACTM, Claas ECJ (2010) Diagnosis of viral gastroenteritis by simultaneous detection of adenovirus group F, astrovirus, rotavirus group A, Norovirus genogroups I and II, and Sapovirus in two internally controlled multiplex real-time PCR assays. J Clin Virol 49:205\u2013210. https:\/\/doi.org\/10.1016\/j.jcv.2010.07.019","journal-title":"J Clin Virol"},{"key":"837_CR27","doi-asserted-by":"publisher","first-page":"255","DOI":"10.7883\/yoken.JJID.2022.704","volume":"76","author":"YH Doan","year":"2023","unstructured":"Doan YH, Yamashita Y, Shinomiya H, Motoya T, Sakon N, Suzuki R et al (2023) Distribution of human Sapovirus strain genotypes over the last four decades in Japan: a global perspective. Jpn J Infect Dis 76:255\u2013258. https:\/\/doi.org\/10.7883\/yoken.JJID.2022.704","journal-title":"Jpn J Infect Dis"},{"key":"837_CR28","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1007\/s10096-019-03697-7","volume":"39","author":"RK Kulis-Horn","year":"2020","unstructured":"Kulis-Horn RK, Tiemann C (2020) Evaluation of a laboratory-developed test for simultaneous detection of Norovirus and rotavirus by real-time RT-PCR on the Panther Fusion\u00ae system. Eur J Clin Microbiol Infect Dis 39:103\u2013112. https:\/\/doi.org\/10.1007\/s10096-019-03697-7","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"837_CR29","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1016\/S1473-3099(18)30362-1","volume":"18","author":"C Troeger","year":"2018","unstructured":"Troeger C, Blacker BF, Khalil IA, Rao PC, Cao S, Zimsen SR et al (2018) Estimates of the global, regional, and National morbidity, mortality, and aetiologies of diarrhoea in 195 countries: a systematic analysis for the global burden of disease study 2016. Lancet Infect Dis 18:1211\u20131228. https:\/\/doi.org\/10.1016\/S1473-3099(18)30362-1","journal-title":"Lancet Infect Dis"},{"key":"837_CR30","doi-asserted-by":"publisher","unstructured":"Hall AJ, Wikswo ME, Manikonda K, Roberts VA, Yoder JS, Gould LH Acute Gastroenteritis Surveillance through the National Outbreak Reporting System, United States - Volume 19, Number 8\u2014August 2013 - Emerging Infectious Diseases journal - CDC n.d. https:\/\/doi.org\/10.3201\/eid1908.130482","DOI":"10.3201\/eid1908.130482"},{"key":"837_CR31","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1097\/QCO.0000000000000557","volume":"32","author":"PP Chong","year":"2019","unstructured":"Chong PP, Atmar RL (2019) Norovirus in health care and implications for the immunocompromised host. Curr Opin Infect Dis 32:348. https:\/\/doi.org\/10.1097\/QCO.0000000000000557","journal-title":"Curr Opin Infect Dis"},{"key":"837_CR32","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1186\/1743-422X-11-129","volume":"11","author":"B-L Rooney","year":"2014","unstructured":"Rooney B-L, Pettipas J, Grudeski E, Mykytczuk O, Pang X-L, Booth TF et al (2014) Detection of Circulating Norovirus genotypes: hitting a moving target. Virol J 11:129. https:\/\/doi.org\/10.1186\/1743-422X-11-129","journal-title":"Virol J"},{"key":"837_CR33","doi-asserted-by":"publisher","first-page":"e0228596","DOI":"10.1371\/journal.pone.0228596","volume":"15","author":"JD Machiels","year":"2020","unstructured":"Machiels JD, Cremers AJH, van Bergen-Verkuyten MCGT, Paardekoper-Strijbosch SJM, Frijns KCJ, Wertheim HFL et al (2020) Impact of the biofire filmarray Gastrointestinal panel on patient care and infection control. PLoS ONE 15:e0228596. https:\/\/doi.org\/10.1371\/journal.pone.0228596","journal-title":"PLoS ONE"},{"key":"837_CR34","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1128\/cmr.00116-13","volume":"27","author":"T Lion","year":"2014","unstructured":"Lion T (2014) Adenovirus infections in immunocompetent and immunocompromised patients. Clin Microbiol Rev 27:441\u2013462. https:\/\/doi.org\/10.1128\/cmr.00116-13","journal-title":"Clin Microbiol Rev"},{"key":"837_CR35","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1038\/leu.2010.4","volume":"24","author":"T Lion","year":"2010","unstructured":"Lion T, Kosulin K, Landlinger C, Rauch M, Preuner S, Jugovic D et al (2010) Monitoring of adenovirus load in stool by real-time PCR permits early detection of impending invasive infection in patients after allogeneic stem cell transplantation. Leukemia 24:706\u2013714. https:\/\/doi.org\/10.1038\/leu.2010.4","journal-title":"Leukemia"},{"key":"837_CR36","doi-asserted-by":"publisher","first-page":"1674","DOI":"10.1111\/j.1469-0691.2011.03488.x","volume":"17","author":"H Jeulin","year":"2011","unstructured":"Jeulin H, Salmon A, Bordigoni P, Venard V (2011) Diagnostic value of quantitative PCR for adenovirus detection in stool samples as compared with antigen detection and cell culture in Haematopoietic stem cell transplant recipients. Clin Microbiol Infect 17:1674\u20131680. https:\/\/doi.org\/10.1111\/j.1469-0691.2011.03488.x","journal-title":"Clin Microbiol Infect"},{"key":"837_CR37","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1186\/s12985-020-01431-w","volume":"17","author":"RS Machado","year":"2020","unstructured":"Machado RS, de Sousa IP, Monteiro JC, Ferreira JL, dos Santos Alves JC, Tavares FN (2020) Detection and identification of enteroviruses Circulating in children with acute gastroenteritis in Par\u00e1 State, Northern Brazil (2010\u20132011). Virol J 17:156. https:\/\/doi.org\/10.1186\/s12985-020-01431-w","journal-title":"Virol J"},{"key":"837_CR38","doi-asserted-by":"publisher","unstructured":"Rojjanadumrongkul K, Kumthip K, Khamrin P, Ukarapol N, Ushijima H, Maneekarn N Enterovirus infections in pediatric patients hospitalized with acute gastroenteritis in Chiang Mai, Thailand, 2015\u20132018. PeerJ 2020;8:e9645. https:\/\/doi.org\/10.7717\/peerj.9645","DOI":"10.7717\/peerj.9645"},{"key":"837_CR39","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.jcv.2018.01.008","volume":"101","author":"H Harvala","year":"2018","unstructured":"Harvala H, Broberg E, Benschop K, Berginc N, Ladhani S, Susi P et al (2018) Recommendations for enterovirus diagnostics and characterisation within and beyond Europe. J Clin Virol 101:11\u201317. https:\/\/doi.org\/10.1016\/j.jcv.2018.01.008","journal-title":"J Clin Virol"},{"key":"837_CR40","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1016\/S1473-3099(23)00066-X","volume":"23","author":"NJ Brendish","year":"2023","unstructured":"Brendish NJ, Beard KR, Malachira AK, Tanner AR, Sanga-Nyirongo L, Gwiggner M et al (2023) Clinical impact of syndromic molecular point-of-care testing for Gastrointestinal pathogens in adults hospitalised with suspected gastroenteritis (GastroPOC): a pragmatic, open-label, randomised controlled trial. Lancet Infect Dis 23:945\u2013955. https:\/\/doi.org\/10.1016\/S1473-3099(23)00066-X","journal-title":"Lancet Infect Dis"},{"key":"837_CR41","doi-asserted-by":"publisher","first-page":"999","DOI":"10.5858\/arpa.2018-0002-OA","volume":"143","author":"J Yoo","year":"2019","unstructured":"Yoo J, Park J, Lee HK, Yu JK, Lee GD, Park KG et al (2019) Comparative evaluation of seegene allplex gastrointestinal, luminex xTAG Gastrointestinal pathogen panel, and BD MAX enteric assays for detection of Gastrointestinal pathogens in clinical stool specimens. Arch Pathol Lab Med 143:999\u20131005. https:\/\/doi.org\/10.5858\/arpa.2018-0002-OA","journal-title":"Arch Pathol Lab Med"}],"container-title":["Medical Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00430-025-00837-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00430-025-00837-z","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00430-025-00837-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:02:47Z","timestamp":1767139367000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00430-025-00837-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,3]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["837"],"URL":"https:\/\/doi.org\/10.1007\/s00430-025-00837-z","relation":{},"ISSN":["0300-8584","1432-1831"],"issn-type":[{"value":"0300-8584","type":"print"},{"value":"1432-1831","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,3]]},"assertion":[{"value":"28 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 May 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 June 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"M.L. received speaker honoraria and related travel expenses from Roche Diagnostics and Qiagen GmbH as well as personal fees for participation on an advisory board from Roche Molecular Systems. D.N. received speaker honoraria and related travel expenses from Roche Diagnostics. The rest of the authors does not declare any competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"28"}}