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In the absence of specific antiviral therapies, the development of novel treatment strategies is crucial. This study evaluates the in vivo antiviral efficacy of 2-BFU, a 2\u2032-\u03b1-fluoro,2\u2032-\u03b2-bromouridine monophosphate prodrug, in a murine model of DENV Serotype 2 (DENV-2) infection. Adult A129 mice were infected subcutaneously with DENV-2 and treated intraperitoneally with 2-BFU at 15\u00a0mg\/kg, starting 12\u00a0h post-infection. Treatment with 2-BFU significantly reduced viral titers in plasma, spleen, and liver, demonstrating potent antiviral activity. Moreover, 2-BFU effectively attenuated DENV-2 -induced thrombocytopenia at 72- and 120-h post-infection. However, the treatment did not significantly affect the production of inflammatory mediators, nor did it prevent infection-associated weight loss or mortality. These findings suggest that 2-BFU holds promise as an antiviral candidate by lowering viral burden and ameliorating thrombocytopenia, although it may require adjunctive anti-inflammatory strategies to improve overall clinical outcomes. Further investigation is warranted to optimize its therapeutic potential for dengue.<\/jats:p>","DOI":"10.1007\/s00430-026-00868-0","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T07:12:18Z","timestamp":1772435538000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The 2\u2032-\u03b1-Fluoro,2\u2032-\u03b2-Bromo uridine phosphoramidate prodrug (2-BFU) reduces dengue virus replication and attenuates infection-induced thrombocytopenia In Vivo"],"prefix":"10.1007","volume":"215","author":[{"given":"Ang\u00e9lica Samer Lallo","family":"Dias","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Rocha da Silva","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenniffer Ramos","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane Lima","family":"Batista","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talita Cristina Martins","family":"da Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia Pereira","family":"Soldati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Martins","family":"Queiroz-Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Amblard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond F.","family":"Schinazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro Martins","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian Vasconcelos","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"868_CR1","doi-asserted-by":"publisher","unstructured":"Bhatt S, Gething PW, Brady OJ et al (2013) The global distribution and burden of dengue. 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