{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T08:15:26Z","timestamp":1775981726705,"version":"3.50.1"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2017,3,17]],"date-time":"2017-03-17T00:00:00Z","timestamp":1489708800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"name":"FP7 European Commission","award":["277775 - IMMOMEC"],"award-info":[{"award-number":["277775 - IMMOMEC"]}]},{"name":"Boehringer Ingelheim RCV"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Cancer Res Clin Oncol"],"published-print":{"date-parts":[[2017,7]]},"DOI":"10.1007\/s00432-017-2399-1","type":"journal-article","created":{"date-parts":[[2017,3,17]],"date-time":"2017-03-17T18:02:46Z","timestamp":1489773766000},"page":"1199-1207","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Non-reproducible sequence artifacts in FFPE tissue: an experience report"],"prefix":"10.1007","volume":"143","author":[{"given":"Richard","family":"Ofner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathrin","family":"Ritter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selma","family":"Ugurel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Cerroni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias","family":"Stiller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Bogenrieder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavio","family":"Solca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Schrama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9183-653X","authenticated-orcid":false,"given":"J\u00fcrgen C.","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,3,17]]},"reference":[{"issue":"7","key":"2399_CR1","doi-asserted-by":"publisher","first-page":"1681","DOI":"10.1016\/j.cell.2015.05.044","volume":"161","author":"R Akbani","year":"2015","unstructured":"Akbani R, Akdemir KC, Aksoy BA, Albert M, Ally A, Amin SB, Zou L (2015) Genomic classification of cutaneous melanoma. Cell 161(7):1681\u20131696. doi: 10.1016\/j.cell.2015.05.044","journal-title":"Cell"},{"key":"2399_CR2","doi-asserted-by":"crossref","unstructured":"Akbari M, Dor\u00e9 Hansen M, Halgunset J, Skorpen F, Krokan HE (2005) Low copy number DNA template can render polymerase chain reaction error prone in a sequence-dependent manner. J Mol Diagn\u2020\u00af 7(1):36\u201339. Retrieved from http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1867510\/","DOI":"10.1016\/S1525-1578(10)60006-2"},{"issue":"1","key":"2399_CR3","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1186\/s12864-015-1982-6","volume":"16","author":"A Astolfi","year":"2015","unstructured":"Astolfi A, Urbini M, Indio V, Nannini M, Genovese CG, Santini D, Pantaleo MA (2015) Whole exome sequencing (WES) on formalin-fixed, paraffin-embedded (FFPE) tumor tissue in gastrointestinal stromal tumors (GIST). BMC Genom 16(1):892. doi: 10.1186\/s12864-015-1982-6","journal-title":"BMC Genom"},{"issue":"7399","key":"2399_CR4","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1038\/nature11071","volume":"485","author":"MF Berger","year":"2012","unstructured":"Berger MF, Hodis E, Heffernan TP, Deribe YL, Lawrence MS, Protopopov A, Garraway LA (2012) Melanoma genome sequencing reveals frequent PREX2 mutations. Nature 485(7399):502\u2013506. doi: 10.1038\/nature11071","journal-title":"Nature"},{"issue":"5","key":"2399_CR5","doi-asserted-by":"publisher","first-page":"e0127146","DOI":"10.1371\/journal.pone.0127146","volume":"10","author":"J Betge","year":"2015","unstructured":"Betge J, Kerr G, Miersch T, Leible S, Erdmann G, Galata CL, Boutros M (2015) Amplicon sequencing of colorectal cancer: variant calling in frozen and formalin-fixed samples. PLoS One 10(5):e0127146. doi: 10.1371\/journal.pone.0127146","journal-title":"PLoS One"},{"key":"2399_CR6","doi-asserted-by":"publisher","first-page":"36540","DOI":"10.1038\/srep36540","volume":"6","author":"L Cai","year":"2016","unstructured":"Cai L, Yuan W, Zhang Z, He L, Chou K-C (2016). In-depth comparison of somatic point mutation callers based on different tumor next-generation sequencing depth data. Sci Rep 6:36540. doi: 10.1038\/srep36540","journal-title":"Sci Rep"},{"issue":"7","key":"2399_CR7","doi-asserted-by":"publisher","first-page":"e0127353","DOI":"10.1371\/journal.pone.0127353","volume":"10","author":"DM Carrick","year":"2015","unstructured":"Carrick DM, Mehaffey MG, Sachs MC, Altekruse S, Camalier C, Chuaqui R, Schully SD (2015) Robustness of next generation sequencing on older formalin-fixed paraffin-embedded tissue. PLoS One 10(7):e0127353. doi: 10.1371\/journal.pone.0127353","journal-title":"PLoS One"},{"issue":"5","key":"2399_CR8","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1007\/s40291-014-0115-2","volume":"18","author":"G Chen","year":"2014","unstructured":"Chen G, Mosier S, Gocke CD, Lin M-T, Eshleman JR (2014). Cytosine deamination is a major cause of baseline noise in next-generation sequencing. Mol Diagn Ther 18(5):587\u2013593. doi: 10.1007\/s40291-014-0115-2","journal-title":"Mol Diagn Ther"},{"issue":"5","key":"2399_CR9","doi-asserted-by":"publisher","first-page":"546","DOI":"10.18632\/oncotarget.503","volume":"3","author":"H Do","year":"2012","unstructured":"Do H, Dobrovic A (2012). Dramatic reduction of sequence artefacts from DNA isolated from formalin-fixed cancer biopsies by treatment with uracil-DNA glycosylase. Oncotarget 3(5):546\u2013558. doi: 10.18632\/oncotarget.503","journal-title":"Oncotarget"},{"issue":"1","key":"2399_CR10","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1373\/clinchem.2014.223040","volume":"61","author":"H Do","year":"2015","unstructured":"Do H, Dobrovic A (2015). Sequence artifacts in DNA from formalin-fixed tissues: causes and strategies for minimization. Clin Chem 61(1):64\u201371. doi: 10.1373\/clinchem.2014.223040","journal-title":"Clin Chem"},{"issue":"2","key":"2399_CR11","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1111\/j.1755-148X.2012.00975.x","volume":"25","author":"K Dutton-Regester","year":"2012","unstructured":"Dutton-Regester K, Hayward NK (2012) Reviewing the somatic genetics of melanoma: from current to future analytical approaches. Pigment Cell Melanoma Res 25(2):144\u2013154. doi: 10.1111\/j.1755-148X.2012.00975.x","journal-title":"Pigment Cell Melanoma Res"},{"issue":"D1","key":"2399_CR12","doi-asserted-by":"publisher","first-page":"D805","DOI":"10.1093\/nar\/gku1075","volume":"43","author":"SA Forbes","year":"2015","unstructured":"Forbes SA, Beare D, Gunasekaran P, Leung K, Bindal N, Boutselakis H, Campbell PJ (2015) COSMIC: exploring the world\u2019s knowledge of somatic mutations in human cancer. Nucleic Acids Res 43(D1):D805\u2013D811. doi: 10.1093\/nar\/gku1075","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"2399_CR13","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1186\/s13073-015-0203-x","volume":"7","author":"J Gagan","year":"2015","unstructured":"Gagan J, Van Allen EM (2015) Next-generation sequencing to guide cancer therapy. Genome Med 7(1):80. doi: 10.1186\/s13073-015-0203-x","journal-title":"Genome Med"},{"key":"2399_CR14","doi-asserted-by":"crossref","unstructured":"Gallegos Ruiz MI, Floor K, Rijmen F, Gr\u00fcnberg K, Rodriguez JA, Giaccone G (2007) EGFR and K-ras mutation analysis in non-small cell lung cancer: comparison of paraffin embedded versus frozen specimens. Cell Oncol\u2020\u00af 29(3):257\u201364. Retrieved from http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=4618423&tool=pmcentrez&rendertype=abstract","DOI":"10.1155\/2007\/568205"},{"issue":"1","key":"2399_CR15","doi-asserted-by":"publisher","first-page":"e009532","DOI":"10.1136\/bmjopen-2015-009532","volume":"6","author":"J Gao","year":"2016","unstructured":"Gao J, Wu H, Wang L, Zhang H, Duan H, Lu J, Liang Z (2016) Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: comparison with Sanger sequencing and ARMS-Scorpion real-time PCR. BMJ Open 6(1):e009532. doi: 10.1136\/bmjopen-2015-009532","journal-title":"BMJ Open"},{"issue":"5","key":"2399_CR16","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1002\/mgg3.223","volume":"4","author":"A Hamilton","year":"2016","unstructured":"Hamilton A, T\u00e9treault M, Dyment DA, Zou R, Kernohan K, Geraghty MT, Boycott KM (2016) Concordance between whole-exome sequencing and clinical Sanger sequencing: implications for patient care. Mol Genet Genom Med 4(5):504\u2013512. doi: 10.1002\/mgg3.223","journal-title":"Mol Genet Genom Med"},{"issue":"25","key":"2399_CR17","doi-asserted-by":"publisher","first-page":"16000","DOI":"10.1073\/pnas.252524999","volume":"99","author":"L Haracska","year":"2002","unstructured":"Haracska L, Prakash L, Prakash S (2002) Role of human DNA polymerase kappa as an extender in translesion synthesis. Proc Natl Acad Sci USA 99(25):16000\u201316005. doi: 10.1073\/pnas.252524999","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5","key":"2399_CR18","doi-asserted-by":"publisher","first-page":"e98187","DOI":"10.1371\/journal.pone.0098187","volume":"9","author":"J Hedegaard","year":"2014","unstructured":"Hedegaard J, Thorsen K, Lund MK, Hein A-MK, Hamilton-Dutoit SJ, Vang S, Dyrskj\u00f8t L (2014) Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue. PLoS One 9(5):e98187. doi: 10.1371\/journal.pone.0098187","journal-title":"PLoS One"},{"issue":"2","key":"2399_CR19","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.cell.2012.06.024","volume":"150","author":"E Hodis","year":"2012","unstructured":"Hodis E, Watson IR, Kryukov GV, Arold ST, Imielinski M, Theurillat J-P, Chin L (2012) A landscape of driver mutations in melanoma. Cell 150(2):251\u2013263. doi: 10.1016\/j.cell.2012.06.024","journal-title":"Cell"},{"issue":"1","key":"2399_CR20","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/1755-8794-4-68","volume":"4","author":"M Kerick","year":"2011","unstructured":"Kerick M, Isau M, Timmermann B, S\u00fcltmann H, Herwig R, Krobitsch S, Schweiger MR (2011) Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity. BMC Med Genom 4(1):68. doi: 10.1186\/1755-8794-4-68","journal-title":"BMC Med Genom"},{"issue":"8","key":"2399_CR21","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1056\/NEJMoa044238","volume":"352","author":"S Kobayashi","year":"2005","unstructured":"Kobayashi S, Boggon TJ, Dayaram T, J\u00e4nne PA, Kocher O, Meyerson M, Halmos B (2005) EGFR mutation and resistance of non-small-cell lung cancer to gefitinib. N Engl J Med 352(8):786\u2013792. doi: 10.1056\/NEJMoa044238","journal-title":"N Engl J Med"},{"issue":"9","key":"2399_CR22","doi-asserted-by":"publisher","first-page":"1006","DOI":"10.1038\/ng.2359","volume":"44","author":"M Krauthammer","year":"2012","unstructured":"Krauthammer M, Kong Y, Ha BH, Evans P, Bacchiocchi A, McCusker JP, Halaban R (2012) Exome sequencing identifies recurrent somatic RAC1 mutations in melanoma. Nat Genet 44(9):1006\u20131014. doi: 10.1038\/ng.2359","journal-title":"Nat Genet"},{"issue":"2","key":"2399_CR23","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.ab.2009.08.016","volume":"395","author":"J Lin","year":"2009","unstructured":"Lin J, Kennedy SH, Svarovsky T, Rogers J, Kemnitz JW, Xu A, Zondervan KT (2009) High-quality genomic DNA extraction from formalin-fixed and paraffin-embedded samples deparaffinized using mineral oil. Anal Biochem 395(2):265\u2013267. doi: 10.1016\/j.ab.2009.08.016","journal-title":"Anal Biochem"},{"issue":"2","key":"2399_CR24","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1007\/s00428-011-1184-9","volume":"460","author":"N Ludyga","year":"2012","unstructured":"Ludyga N, Gr\u00fcnwald B, Azimzadeh O, Englert S, H\u00f6fler H, Tapio S, Aubele M (2012). Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses. Virchows Arch 460(2):131\u2013140. doi: 10.1007\/s00428-011-1184-9","journal-title":"Virchows Arch"},{"issue":"12","key":"2399_CR25","doi-asserted-by":"publisher","first-page":"e0144162","DOI":"10.1371\/journal.pone.0144162","volume":"10","author":"E Oh","year":"2015","unstructured":"Oh E, Choi Y-L, Kwon MJ, Kim RN, Kim YJ, Song J-Y, Shin YK (2015) Comparison of accuracy of whole-exome sequencing with formalin-fixed paraffin-embedded and fresh frozen tissue samples. PLoS One 10(12):e0144162. doi: 10.1371\/journal.pone.0144162","journal-title":"PLoS One"},{"issue":"2","key":"2399_CR26","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1093\/bib\/bbs086","volume":"15","author":"S Pabinger","year":"2014","unstructured":"Pabinger S, Dander A, Fischer M, Snajder R, Sperk M, Efremova M, Trajanoski Z (2014) A survey of tools for variant analysis of next-generation genome sequencing data. Brief Bioinform 15(2):256\u2013278. doi: 10.1093\/bib\/bbs086","journal-title":"Brief Bioinform"},{"issue":"3","key":"2399_CR27","doi-asserted-by":"publisher","first-page":"e73","DOI":"10.1371\/journal.pmed.0020073","volume":"2","author":"W Pao","year":"2005","unstructured":"Pao W, Miller VA, Politi KA, Riely GJ, Somwar R, Zakowski MF, Varmus H (2005) Acquired resistance of lung adenocarcinomas to gefitinib or erlotinib is associated with a second mutation in the EGFR kinase domain. PLoS Med 2(3):e73. doi: 10.1371\/journal.pmed.0020073","journal-title":"PLoS Med"},{"issue":"7278","key":"2399_CR28","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1038\/nature08658","volume":"463","author":"ED Pleasance","year":"2010","unstructured":"Pleasance ED, Cheetham RK, Stephens PJ, McBride DJ, Humphray SJ, Greenman CD, Stratton MR (2010) A comprehensive catalogue of somatic mutations from a human cancer genome. Nature 463(7278):191\u2013196. doi: 10.1038\/nature08658","journal-title":"Nature"},{"issue":"8 Supplement","key":"2399_CR29","doi-asserted-by":"publisher","first-page":"LB-134-LB-134","DOI":"10.1158\/1538-7445.AM10-LB-134","volume":"70","author":"DK Pokholok","year":"2014","unstructured":"Pokholok DK, Le JM, Steemers FJ, Ronaghi M, Gunderson KL (2014) Abstract LB-134: analysis of restored FFPE samples on high-density SNP arrays. Cancer Res 70(8 Supplement):LB-134-LB-134. doi: 10.1158\/1538-7445.AM10-LB-134","journal-title":"Cancer Res"},{"issue":"10","key":"2399_CR30","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1038\/ng.438","volume":"41","author":"TD Prickett","year":"2009","unstructured":"Prickett TD, Agrawal NS, Wei X, Yates KE, Lin JC, Wunderlich JR, Samuels Y (2009) Analysis of the tyrosine kinome in melanoma reveals recurrent mutations in ERBB4. Nat Genet 41(10):1127\u20131132. doi: 10.1038\/ng.438","journal-title":"Nat Genet"},{"key":"2399_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1472-6890-4-1","volume":"4","author":"N Quach","year":"2004","unstructured":"Quach N, Goodman MF, Shibata D (2004) In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR. BMC Clin Pathol 4:1. doi: 10.1186\/1472-6890-4-1","journal-title":"BMC Clin Pathol"},{"issue":"8","key":"2399_CR32","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/gm481","volume":"5","author":"S Sah","year":"2013","unstructured":"Sah S, Chen L, Houghton J, Kemppainen J, Marko AC, Zeigler R, Latham GJ (2013). Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies. Genome Med 5(8):77. doi: 10.1186\/gm481","journal-title":"Genome Med"},{"issue":"12","key":"2399_CR33","doi-asserted-by":"publisher","first-page":"1667","DOI":"10.1002\/pro.2554","volume":"23","author":"N Schormann","year":"2014","unstructured":"Schormann N, Ricciardi R, Chattopadhyay D (2014) Uracil-DNA glycosylases-structural and functional perspectives on an essential family of DNA repair enzymes. Protein Sci\u2020\u00af Publ Protein Soc 23(12):1667\u20131685. doi: 10.1002\/pro.2554","journal-title":"Protein Sci\u2020\u00af Publ Protein Soc"},{"issue":"5","key":"2399_CR34","doi-asserted-by":"publisher","first-page":"e5548","DOI":"10.1371\/journal.pone.0005548","volume":"4","author":"MR Schweiger","year":"2009","unstructured":"Schweiger MR, Kerick M, Timmermann B, Albrecht MW, Borodina T, Parkhomchuk D, Lehrach H (2009) Genome-wide massively parallel sequencing of formaldehyde fixed-paraffin embedded (FFPE) tumor tissues for copy-number- and mutation-analysis. PLoS One 4(5):e5548. doi: 10.1371\/journal.pone.0005548","journal-title":"PLoS One"},{"issue":"1","key":"2399_CR35","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/1480-9222-15-5","volume":"15","author":"T Sedlackova","year":"2013","unstructured":"Sedlackova T, Repiska G, Celec P, Szemes T, Minarik G (2013) Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods. Biol Proced Online 15(1):5. doi: 10.1186\/1480-9222-15-5","journal-title":"Biol Proced Online"},{"issue":"5","key":"2399_CR36","doi-asserted-by":"publisher","first-page":"3191","DOI":"10.3390\/ijms12053191","volume":"12","author":"J Solassol","year":"2011","unstructured":"Solassol J, Ramos J, Crapez E, Saifi M, Mang\u00e9 A, Vian\u00e8s E, Maudelonde T (2011) KRAS mutation detection in paired frozen and formalin-fixed paraffin-embedded (FFPE) colorectal cancer tissues. Int J Mol Sci 12(5):3191\u20133204. doi: 10.3390\/ijms12053191","journal-title":"Int J Mol Sci"},{"issue":"5","key":"2399_CR37","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/j.jmoldx.2013.05.004","volume":"15","author":"DH Spencer","year":"2013","unstructured":"Spencer DH, Sehn JK, Abel HJ, Watson MA, Pfeifer JD, Duncavage EJ (2013) Comparison of clinical targeted next-generation sequence data from formalin-fixed and fresh-frozen tissue specimens. J Mol Diagn\u2020\u00af 15(5):623\u2013633. doi: 10.1016\/j.jmoldx.2013.05.004","journal-title":"J Mol Diagn\u2020\u00af"},{"key":"2399_CR38","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10827","author":"M Stiller","year":"2016","unstructured":"Stiller M, Sucker A, Griewank K, Aust D, Baretton GB, Schadendorf D, Horn S (2016). Single-strand DNA library preparation improves sequencing of formalin-fixed and paraffin-embedded (FFPE) cancer DNA. Oncotarget. doi: 10.18632\/oncotarget.10827","journal-title":"Oncotarget"},{"issue":"1","key":"2399_CR39","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1038\/jid.2013.272","volume":"134","author":"RA Sturm","year":"2014","unstructured":"Sturm RA, Fox C, McClenahan P, Jagirdar K, Ibarrola-Villava M, Banan P, Soyer HP (2014) Phenotypic characterization of nevus and tumor patterns in MITF E318K mutation carrier melanoma patients. J Invest Dermatol 134(1):141\u2013149. doi: 10.1038\/jid.2013.272","journal-title":"J Invest Dermatol"},{"issue":"suppl 2","key":"2399_CR40","doi-asserted-by":"publisher","first-page":"W71","DOI":"10.1093\/nar\/gkm306","volume":"35","author":"A Untergasser","year":"2007","unstructured":"Untergasser A, Nijveen H, Rao X, Bisseling T, Geurts R, Leunissen JAM (2007) Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res 35(suppl 2):W71\u2013W74. doi: 10.1093\/nar\/gkm306","journal-title":"Nucleic Acids Res"},{"key":"2399_CR41","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/1755-8794-7-23","volume":"7","author":"SQ Wong","year":"2014","unstructured":"Wong SQ, Li J, Tan A Y-C, Vedururu R, Pang J-MB, Do H, Dobrovic A (2014) Sequence artefacts in a prospective series of formalin-fixed tumours tested for mutations in hotspot regions by massively parallel sequencing. BMC Med Genom 7:23. doi: 10.1186\/1755-8794-7-23","journal-title":"BMC Med Genom"},{"issue":"9","key":"2399_CR42","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1097\/JTO.0b013e31829f691f","volume":"8","author":"X Ye","year":"2013","unstructured":"Ye X, Zhu Z-Z, Zhong L, Lu Y, Sun Y, Yin X, Ji Q (2013) High T790M detection rate in TKI-naive NSCLC with EGFR sensitive mutation: truth or artifact? J Thorac Oncol\u2020\u00af 8(9):1118\u20131120. doi: 10.1097\/JTO.0b013e31829f691f","journal-title":"J Thorac Oncol\u2020\u00af"}],"container-title":["Journal of Cancer Research and Clinical Oncology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00432-017-2399-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00432-017-2399-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00432-017-2399-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T18:02:59Z","timestamp":1692727379000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00432-017-2399-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,17]]},"references-count":42,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["2399"],"URL":"https:\/\/doi.org\/10.1007\/s00432-017-2399-1","relation":{},"ISSN":["0171-5216","1432-1335"],"issn-type":[{"value":"0171-5216","type":"print"},{"value":"1432-1335","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,17]]}}}