{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:18:47Z","timestamp":1792181927225,"version":"4.4.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100012515","name":"Cyrus Tang Foundation","doi-asserted-by":"publisher","award":["ZSBK0070"],"award-info":[{"award-number":["ZSBK0070"]}],"id":[{"id":"10.13039\/100012515","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Municipal Key Clinical Specialty","award":["no. shslczdzk05703"],"award-info":[{"award-number":["no. shslczdzk05703"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["no. 82072782"],"award-info":[{"award-number":["no. 82072782"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008750","name":"Shanghai Hospital Development Center","doi-asserted-by":"publisher","award":["Grant No. SHDC12018X22"],"award-info":[{"award-number":["Grant No. SHDC12018X22"]}],"id":[{"id":"10.13039\/501100008750","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Children's National Medical Center","award":["Grant No. EK112520180301"],"award-info":[{"award-number":["Grant No. EK112520180301"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Cancer Res Clin Oncol"],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1007\/s00432-022-04073-3","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T11:03:40Z","timestamp":1655463820000},"page":"3267-3280","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Single-cell transcriptional profiling reveals heterogeneity and developmental trajectories of Ewing sarcoma"],"prefix":"10.1007","volume":"148","author":[{"given":"Bo","family":"Hong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ShuYang","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9645-4768","authenticated-orcid":false,"given":"Rui","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"4073_CR1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.51576","author":"E Barruet","year":"2020","unstructured":"Barruet E, Garcia SM, Striedinger K et al (2020) Functionally heterogeneous human satellite cells identified by single cell RNA sequencing. Elife. https:\/\/doi.org\/10.7554\/eLife.51576","journal-title":"Elife"},{"key":"4073_CR2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-14-32","author":"M Brentnall","year":"2013","unstructured":"Brentnall M, Rodriguez-Menocal L, de Guevara RL et al (2013) Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis. BMC Cell Biol. https:\/\/doi.org\/10.1186\/1471-2121-14-32","journal-title":"BMC Cell Biol"},{"key":"4073_CR3","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-018-0456-5","author":"L Cheng","year":"2019","unstructured":"Cheng L, Pandya PH, Liu E et al (2019) Integration of genomic copy-number variations and chemotherapy-response biomarkers in pediatric sarcoma. BMC Med Genom. https:\/\/doi.org\/10.1186\/s12920-018-0456-5","journal-title":"BMC Med Genom"},{"key":"4073_CR4","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.wneu.2018.04.164","volume":"115","author":"A Cherif El Asri","year":"2018","unstructured":"Cherif El Asri A, Benzagmout M, Chakour K et al (2018) Primary intracranial pPNET\/Ewing sarcoma: diagnosis, management, and prognostic factors dilemma\u2014a systematic review of the literature. World Neurosurg 115:346","journal-title":"World Neurosurg"},{"key":"4073_CR5","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13040879","author":"G Domenici","year":"2021","unstructured":"Domenici G, Eduardo R, Castillo-Ecija H et al (2021) Pdx-derived Ewing\u2019s sarcoma cells retain high viability and disease phenotype in alginate encapsulated spheroid cultures. Cancers (basel). https:\/\/doi.org\/10.3390\/cancers13040879","journal-title":"Cancers (basel)"},{"key":"4073_CR6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.08.014","author":"R Dong","year":"2020","unstructured":"Dong R, Yang R, Zhan Y et al (2020) Single-cell characterization of malignant phenotypes and developmental trajectories of adrenal neuroblastoma. Cancer Cell. https:\/\/doi.org\/10.1016\/j.ccell.2020.08.014","journal-title":"Cancer Cell"},{"key":"4073_CR7","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00794-1","author":"UC Eze","year":"2021","unstructured":"Eze UC, Bhaduri A, Haeussler M et al (2021) Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia. Nat Neurosci. https:\/\/doi.org\/10.1038\/s41593-020-00794-1","journal-title":"Nat Neurosci"},{"key":"4073_CR8","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-021-00163-z","author":"Y Gan","year":"2021","unstructured":"Gan Y, He J, Zhu J et al (2021) Spatially defined single-cell transcriptional profiling characterizes diverse chondrocyte subtypes and nucleus pulposus progenitors in human intervertebral discs. Bone Res. https:\/\/doi.org\/10.1038\/s41413-021-00163-z","journal-title":"Bone Res"},{"key":"4073_CR9","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-00795-2","author":"R Gao","year":"2021","unstructured":"Gao R, Bai S, Henderson YC et al (2021) Delineating copy number and clonal substructure in human tumors from single-cell transcriptomes. Nat Biotechnol. https:\/\/doi.org\/10.1038\/s41587-020-00795-2","journal-title":"Nat Biotechnol"},{"key":"4073_CR10","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203452","author":"Y Gong","year":"2021","unstructured":"Gong Y, Yang J, Li X et al (2021) A systematic dissection of human primary osteoblasts in vivo at single-cell resolution. Aging. https:\/\/doi.org\/10.18632\/aging.203452","journal-title":"Aging"},{"key":"4073_CR11","doi-asserted-by":"crossref","unstructured":"Gr\u00fcnewald TGP, Cidre-Aranaz F, Surdez D et al (2018) Ewing sarcoma. Nat Rev Dis Prim 4","DOI":"10.1038\/s41572-018-0003-x"},{"key":"4073_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048","author":"Y Hao","year":"2021","unstructured":"Hao Y, Hao S, Andersen-Nissen E et al (2021) Integrated analysis of multimodal single-cell data. Cell. https:\/\/doi.org\/10.1016\/j.cell.2021.04.048","journal-title":"Cell"},{"key":"4073_CR13","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1434-6","author":"V Hovestadt","year":"2019","unstructured":"Hovestadt V, Smith KS, Bihannic L et al (2019) Resolving medulloblastoma cellular architecture by single-cell genomics. Nature. https:\/\/doi.org\/10.1038\/s41586-019-1434-6","journal-title":"Nature"},{"key":"4073_CR14","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0619-0","author":"I Korsunsky","year":"2019","unstructured":"Korsunsky I, Millard N, Fan J et al (2019) Fast, sensitive and accurate integration of single-cell data with Harmony. Nat Methods. https:\/\/doi.org\/10.1038\/s41592-019-0619-0","journal-title":"Nat Methods"},{"key":"4073_CR15","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9050368","author":"BD la Fuente","year":"2020","unstructured":"la Fuente BD, L\u00f3pez-Garc\u00eda G, M\u00e1\u00f1ez V et al (2020) Antiproliferative effect of bioaccessible fractions of four Brassicaceae microgreens on human colon cancer cells linked to their phytochemical composition. Antioxidants. https:\/\/doi.org\/10.3390\/antiox9050368","journal-title":"Antioxidants"},{"key":"4073_CR16","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-72680-6","author":"Y Liu","year":"2020","unstructured":"Liu Y, Yuan Y, Zhang F et al (2020) Outcome of multidisciplinary treatment of peripheral primitive neuroectodermal tumor. Sci Rep. https:\/\/doi.org\/10.1038\/s41598-020-72680-6","journal-title":"Sci Rep"},{"key":"4073_CR17","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.606456","author":"C Mazewski","year":"2020","unstructured":"Mazewski C, Perez RE, Fish EN et al (2020) Type I interferon (IFN)-regulated activation of canonical and non-canonical signaling pathways. Front Immunol. https:\/\/doi.org\/10.3389\/fimmu.2020.606456","journal-title":"Front Immunol"},{"key":"4073_CR18","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2021.751204","author":"L Meng","year":"2021","unstructured":"Meng L, Liu J, Wang J et al (2021) Characteristics of the gut microbiome and IL-13\/TGF-\u03b21 mediated fibrosis in post-kasai cholangitis of biliary atresia. Front Pediatr. https:\/\/doi.org\/10.3389\/fped.2021.751204","journal-title":"Front Pediatr"},{"key":"4073_CR19","doi-asserted-by":"publisher","DOI":"10.4103\/jcvjs.JCVJS_70_17","author":"S Munjal","year":"2017","unstructured":"Munjal S, Srivastava A, Tucker S et al (2017) Primary dorsal spine primitive neuroectodermal tumor in an adult patient: case report and literature review. J Cranioverteb Junct Spine. https:\/\/doi.org\/10.4103\/jcvjs.JCVJS_70_17","journal-title":"J Cranioverteb Junct Spine"},{"key":"4073_CR20","doi-asserted-by":"publisher","DOI":"10.3171\/PED-07\/11\/411","author":"R Navarro","year":"2007","unstructured":"Navarro R, Laguna A, de Torres C et al (2007) Primary Ewing sarcoma of the tentorium presenting with intracranial hemorrhage in a child. J Neurosurg. https:\/\/doi.org\/10.3171\/PED-07\/11\/411","journal-title":"J Neurosurg"},{"key":"4073_CR21","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22083","author":"AR Paek","year":"2014","unstructured":"Paek AR, Lee CH, You HJ (2014) A role of zinc-finger protein 143 for cancer cell migration and invasion through ZEB1 and E-cadherin in colon cancer cells. Mol Carcinog. https:\/\/doi.org\/10.1002\/mc.22083","journal-title":"Mol Carcinog"},{"key":"4073_CR22","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4150","author":"X Qiu","year":"2017","unstructured":"Qiu X, Hill A, Packer J et al (2017) Single-cell mRNA quantification and differential analysis with Census. Nat Methods. https:\/\/doi.org\/10.1038\/nmeth.4150","journal-title":"Nat Methods"},{"key":"4073_CR23","doi-asserted-by":"publisher","DOI":"10.20517\/2394-4722.2020.58","author":"Z Qureshy","year":"2020","unstructured":"Qureshy Z, Johnson DE, Grandis JR (2020) Targeting the JAK\/STAT pathway in solid tumors. J Cancer Metastasis Treat.\u00a0https:\/\/doi.org\/10.20517\/2394-4722.2020.58","journal-title":"J Cancer Metastasis Treat"},{"key":"4073_CR24","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-021-00619-8","author":"EA Roundhill","year":"2021","unstructured":"Roundhill EA, Chicon-Bosch M, Jeys L et al (2021) RNA sequencing and functional studies of patient-derived cells reveal that neurexin-1 and regulators of this pathway are associated with poor outcomes in Ewing sarcoma. Cell Oncol. https:\/\/doi.org\/10.1007\/s13402-021-00619-8","journal-title":"Cell Oncol"},{"key":"4073_CR25","doi-asserted-by":"publisher","DOI":"10.4103\/2152-7806.96154","author":"S Saeedinia","year":"2012","unstructured":"Saeedinia S, Nouri M, Alimohammadi M et al (2012) Primary spinal extradural Ewing\u2019s sarcoma (primitive neuroectodermal tumor): report of a case and meta-analysis of the reported cases in the literature. Surg Neurol Int. https:\/\/doi.org\/10.4103\/2152-7806.96154","journal-title":"Surg Neurol Int"},{"key":"4073_CR26","doi-asserted-by":"publisher","DOI":"10.1126\/science.aai8478","author":"AS Venteicher","year":"2017","unstructured":"Venteicher AS, Tirosh I, Hebert C et al (2017) Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq. Science. https:\/\/doi.org\/10.1126\/science.aai8478","journal-title":"Science"},{"key":"4073_CR27","doi-asserted-by":"publisher","DOI":"10.1101\/2020.04.06.027904","author":"Z Wang","year":"2020","unstructured":"Wang Z, Li X, Yang J et al (2020) Single-cell RNA sequencing deconvolutes the in vivo heterogeneity of human bone marrow-derived mesenchymal stem cells. bioRrxiv. https:\/\/doi.org\/10.1101\/2020.04.06.027904","journal-title":"bioRrxiv"},{"key":"4073_CR28","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141","author":"T Wu","year":"2021","unstructured":"Wu T, Hu E, Xu S et al (2021) clusterProfiler 4.0: a universal enrichment tool for interpreting omics data. Innovation (china). https:\/\/doi.org\/10.1016\/j.xinn.2021.100141","journal-title":"Innovation (china)"},{"key":"4073_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.05.144","author":"CJ Ye","year":"2020","unstructured":"Ye CJ, Zhan Y, Yang R et al (2020) Single-cell transcriptional profiling identifies a cluster of potential metastasis-associated UBE2C+ cells in immature ovarian teratoma. Biochem Biophys Res Commun. https:\/\/doi.org\/10.1016\/j.bbrc.2020.05.144","journal-title":"Biochem Biophys Res Commun"}],"container-title":["Journal of Cancer Research and Clinical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00432-022-04073-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00432-022-04073-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00432-022-04073-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T03:07:18Z","timestamp":1666321638000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00432-022-04073-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,17]]},"references-count":29,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["4073"],"URL":"https:\/\/doi.org\/10.1007\/s00432-022-04073-3","relation":{},"ISSN":["0171-5216","1432-1335"],"issn-type":[{"value":"0171-5216","type":"print"},{"value":"1432-1335","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,17]]},"assertion":[{"value":"2 November 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 May 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 June 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study was approved by the ethics committee of Fudan University Children\u2019s Hospital.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"All the authors consent for publication, and informed consent was obtained from the participants\u2019 guardians. All the experiments were carried out in accordance with the relevant guidelines and regulations.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}]}}