{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T08:42:09Z","timestamp":1783068129549,"version":"3.54.6"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2009,5,5]],"date-time":"2009-05-05T00:00:00Z","timestamp":1241481600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2009,8]]},"DOI":"10.1007\/s00436-009-1443-8","type":"journal-article","created":{"date-parts":[[2009,5,4]],"date-time":"2009-05-04T21:05:11Z","timestamp":1241471111000},"page":"721-730","source":"Crossref","is-referenced-by-count":55,"title":["Detection of tick blood parasites in Egypt using PCR assay I\u2014Babesia bovis and Babesia bigemina"],"prefix":"10.1007","volume":"105","author":[{"given":"F. K.","family":"Adham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. M.","family":"Abd-El-Samie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. M.","family":"Gabre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. El.","family":"Hussein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2009,5,5]]},"reference":[{"key":"1443_CR1","unstructured":"Abd-El Gawad M (1993) Some studies on Babesia species in cattle in Beni-Suef governorate. Ph.D. Thesis, Fac. Vet. Med., Cairo Univ., Giza, Egypt"},{"key":"1443_CR2","unstructured":"Abo-Elkheir EA (1989) Epidemiological aspect on the distribution of haemotrophic parasites among cattle in Upper Egypt confirmed by the identification of encountered organisms. M.Sc. Thesis, Fac. Vet. Med., Cairo Univ., Giza, Egypt"},{"key":"1443_CR3","unstructured":"Ahmed AB (1980) Some biological studies on blood parasites of farm animals. Ph.D. Thesis, Fac. Vet. Med., Zagazig Univ., Zagazig, Egypt"},{"key":"1443_CR4","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"SF Altschul","year":"1997","unstructured":"Altschul SF, Madden TL, Sch\u00e4fferl AA, Zhang J, Zheng Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389\u20133402","journal-title":"Nucleic Acids Res"},{"key":"1443_CR5","unstructured":"Calder JA (1994) Improved diagnosis of bovine babesiosis. Ph.D. Thesis, Fac. Vet. Med. Florida Univ., Gainesville, FL, USA"},{"key":"1443_CR6","unstructured":"Chafick ML (1987) Serological tests for the field diagnosis of Babesia infection in claves. M.Sc. Thesis, Fac. Vet. Med., Cairo Univ., Cairo, Egypt"},{"key":"1443_CR7","unstructured":"Drummond RO, George JE, Kunz SE (ed) (1988) Control of arthropod pests of livestock. Rev. Tech., CRC Press, Boca Raton, pp 1\u201319 and 145\u2013158"},{"key":"1443_CR8","unstructured":"El-Allawy TA (1973) Some studies on bovine piroplasmosis in Assiut. M.Sc. Thesis, Fac. Vet. Med., Assiut Univ., Assiut, Egypt"},{"key":"1443_CR9","unstructured":"El-Ghaysh AA (1993) Studies on Babesia sp. infecting cattle and water buffaloes in Egypt. Ph.D. Thesis, Fac. Vet. Med., Cairo Univ. Giza, Egypt"},{"key":"1443_CR10","unstructured":"Fadly RS (1996) Serological studies on Babesia in Behera Province M.Sc. Thesis, Fac. Vet. Med. Alexandria Univ. Alexandria, Egypt"},{"key":"1443_CR11","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1128\/JCM.30.6.1374-1379.1992","volume":"30","author":"Y Fahrimal","year":"1992","unstructured":"Fahrimal Y, Goff WL, Jasmer DP (1992) Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA. J Clin Microbiol 30:2091\u20132096","journal-title":"J Clin Microbiol"},{"key":"1443_CR12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0304-4017(93)90008-B","volume":"50","author":"JV Figueroa","year":"1993","unstructured":"Figueroa JV, Chieves LP, Johnson GS, Buening GM (1993) Multiplex polymerase chain reaction based assay for detection of Babesia bigemina, Babesia bovis and Anaplasma marginale DNA in bovine blood. Vet Parasitol 50:69\u201381","journal-title":"Vet Parasitol"},{"key":"1443_CR13","unstructured":"Gattas MW (1983) Some studies on cattle Babesiosis in Suez Canal zone. M.Sc. Thesis, Fac. Vet. Med., Cairo Univ., Giza, Egypt"},{"key":"1443_CR14","unstructured":"Gattas MW (1990) Further studies on the biological aspect of Babesia species in Suez Canal zone with special references to their control. Ph.D. Thesis, Cairo Univ., Giza, Egypt"},{"key":"1443_CR15","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1093\/jmedent\/32.3.213","volume":"32","author":"JA Higgins","year":"1995","unstructured":"Higgins JA, Azad AF (1995) Use of polymerase chain reaction to detect bacteria in arthropods: a review. J Med Entomol 32:213\u2013222","journal-title":"J Med Entomol"},{"key":"1443_CR16","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1146\/annurev.en.26.010181.000451","volume":"26","author":"H Hoogstraal","year":"1981","unstructured":"Hoogstraal H (1981) Changing patterns of tick borne diseases in modern society. Ann Rev Entomol 26:75\u201399","journal-title":"Ann Rev Entomol"},{"key":"1443_CR17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00034983.1969.11686595","volume":"63","author":"DF Mahoney","year":"1969","unstructured":"Mahoney DF (1969) Bovine babesiosis: a study of factors concerned in transmission. Ann Trop Parasitol 63:1\u201314","journal-title":"Ann Trop Parasitol"},{"key":"1443_CR18","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.vetpar.2008.01.037","volume":"153","author":"TM Martins","year":"2008","unstructured":"Martins TM, Pedro OC, Caldeira RA, Do Ros\u00e1rio VE, Neves L, Domingos A (2008) Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method. Vet Parasitol 153:225\u2013230","journal-title":"Vet Parasitol"},{"key":"1443_CR19","first-page":"647","volume":"38","author":"RN Mohan","year":"1968","unstructured":"Mohan RN (1968) Diseases and parasites of buffaloes. Vet Bull 38:647\u2013659","journal-title":"Vet Bull"},{"issue":"Suppl 3","key":"1443_CR20","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1590\/S0074-02761992000700033","volume":"87","author":"S Morzaria","year":"1992","unstructured":"Morzaria S, Katende J, Kairo A, Nene V, Musoke A (1992) New methods for the diagnosis of Babesia bigemina infection. Mem Inst Oswaldo Cruz Rio J 87(Suppl 3):201\u2013205","journal-title":"Mem Inst Oswaldo Cruz Rio J"},{"key":"1443_CR21","first-page":"145","volume":"59","author":"HE Nagati","year":"1947","unstructured":"Nagati HE (1947) Some new and rare records of piroplasmosis with a list of the species of Babesia and Theileria so far recorded from Egypt. Vet Rec 59:145\u2013147","journal-title":"Vet Rec"},{"key":"1443_CR22","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.ijpara.2004.09.002","volume":"35","author":"TCG Oliveira-Sequeira","year":"2005","unstructured":"Oliveira-Sequeira TCG, Oliveira MCS, Araujo JP, Amarante AFT (2005) PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle. Int J Parasitol 35:105\u2013111","journal-title":"Int J Parasitol"},{"key":"1443_CR23","doi-asserted-by":"crossref","first-page":"3641","DOI":"10.1093\/nar\/19.13.3641","volume":"19","author":"GR Reddy","year":"1991","unstructured":"Reddy GR, Charkarbari D, Yowell CA, Dame JB (1991) Sequence microheterogeneity of the three small subunit ribosomal RNA genes of B. bigemina expression in erythrocyte culture. Nucleic Acids Res 19:3641\u20133645","journal-title":"Nucleic Acids Res"},{"key":"1443_CR24","first-page":"644","volume-title":"Foundations of parasitology","author":"LS Roberts","year":"2005","unstructured":"Roberts LS, Janovy J (2005) Foundations of parasitology, 7th edn. McGraw Hill, New York, pp 644\u2013646","edition":"7"},{"key":"1443_CR25","first-page":"488","volume":"23","author":"RK Saiki","year":"1988","unstructured":"Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 23:488\u2013491","journal-title":"Science"},{"key":"1443_CR26","unstructured":"Sakla AA (1975) Studies on ticks in Assiut Governorate, with special reference to their role in transmission of parasitic diseases. M.Sc. Thesis, Fac. Vet. Med., Assiut Univ., Assiut, Egypt"},{"key":"1443_CR27","first-page":"92","volume":"39","author":"GH Salem","year":"1998","unstructured":"Salem GH (1998) Using of ribosomal DNA based PCR method for sensitive detection of carrier and mild babesial infection among cattle in Egypt. Assiut Vet Med J 39:92\u2013108","journal-title":"Assiut Vet Med J"},{"key":"1443_CR28","volume-title":"Molecular cloning: a laboratory manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor"},{"key":"1443_CR29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF02383236","volume":"7","author":"RA Todorovic","year":"1975","unstructured":"Todorovic RA (1975) Serological diagnosis of babesiosis: a review. Trop Anim Health Prod 7:1\u201314","journal-title":"Trop Anim Health Prod"},{"key":"1443_CR30","doi-asserted-by":"crossref","unstructured":"Wall R, Shearer D (ed) (1997) Veterinary entomology. Chapman and Hall, London, pp 97\u2013140 and 132\u2013133","DOI":"10.1007\/978-94-011-5852-7"},{"key":"1443_CR31","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/0169-4758(90)90071-B","volume":"6","author":"AP Waters","year":"1990","unstructured":"Waters AP, McCutchan TF (1990) Ribosomal RNA, nature\u2019s own polymerase-amplified target for diagnosis. Parasit Today 6:56\u201359","journal-title":"Parasit Today"},{"key":"1443_CR32","first-page":"143","volume-title":"Babesiosis of domestic animals and man","author":"G Weiland","year":"1988","unstructured":"Weiland G, Reiter I (1988) Methods for measurement of the serological response to Babesia. In: Ristic M (ed) Babesiosis of domestic animals and man. CRC Press, Boca Raton, pp 143\u2013162"},{"key":"1443_CR33","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0934-8840(11)80568-2","volume":"281","author":"MM Wittenbrink","year":"1994","unstructured":"Wittenbrink MM, Thiele D, Krauss H (1994) Comparison of dark-field microscopy, culture, and polymerase chain reaction (PCR) for detection of Borrelia burgdorferi in field-collected Ixodes ricinus ticks. Zentralbl Bakteriol 281:183\u2013191","journal-title":"Zentralbl Bakteriol"},{"key":"1443_CR34","first-page":"263","volume":"39","author":"M Zhang","year":"1995","unstructured":"Zhang M, Gothe R, Rinder H (1995) Detection of spotted fever group rickettsiae in ticks and rodents by PCR technique in People\u2019s Republic of China. Acta Virol 39:263\u2013267","journal-title":"Acta Virol"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-009-1443-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00436-009-1443-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-009-1443-8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T22:40:47Z","timestamp":1589755247000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00436-009-1443-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5,5]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["1443"],"URL":"https:\/\/doi.org\/10.1007\/s00436-009-1443-8","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5,5]]}}}