{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:19:45Z","timestamp":1790835585833,"version":"4.1.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2011,7,23]],"date-time":"2011-07-23T00:00:00Z","timestamp":1311379200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2012,2]]},"DOI":"10.1007\/s00436-011-2557-3","type":"journal-article","created":{"date-parts":[[2011,7,22]],"date-time":"2011-07-22T11:46:14Z","timestamp":1311335174000},"page":"799-808","source":"Crossref","is-referenced-by-count":25,"title":["Light and electron microscopy observations of embryogenesis and egg development in the human liver fluke, Opisthorchis viverrini (Platyhelminthes, Digenea)"],"prefix":"10.1007","volume":"110","author":[{"given":"Panita","family":"Khampoosa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm K.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica M.","family":"Lovas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuanchai","family":"Srisawangwong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thewarach","family":"Laha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supawadee","family":"Piratae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chalida","family":"Thammasiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apiporn","family":"Suwannatrai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bungorn","family":"Sripanidkulchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veerachai","family":"Eursitthichai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smarn","family":"Tesana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,7,23]]},"reference":[{"key":"2557_CR1","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1017\/S0031182001007351","volume":"122","author":"PD Ashton","year":"2001","unstructured":"Ashton PD, Harrop R, Shah B, Wilson RA (2001) The schistosome egg: development and secretions. Parasitology 122:329\u2013338","journal-title":"Parasitology"},{"key":"2557_CR2","first-page":"18","volume":"2","author":"SA Beer","year":"1991","unstructured":"Beer SA, German SM, Ditrich O, Giboda M (1991) The morphofunctional characteristics of the ultrastructure of the outer membrane of opisthorchid eggs. 1. The hatching of miracidia. Med Parazitol (Mosk) 2:18\u201322","journal-title":"Med Parazitol (Mosk)"},{"key":"2557_CR3","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s00436-008-1236-5","volume":"104","author":"G Cai","year":"2008","unstructured":"Cai G, Bae Y, Zhang Y, He Y, Jiang M, He L (2008) Expression and characterization of two tyrosinases from the trematode Schistosoma japonicum. Parasitol Res 104:601\u2013609","journal-title":"Parasitol Res"},{"key":"2557_CR4","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1017\/S0031182098002662","volume":"116","author":"LM Colhoun","year":"1998","unstructured":"Colhoun LM, Fairweather I, Brennan GP (1998) Observations on the mechanism of eggshell formation in the liver fluke, Fasciola hepatica. Parasitology 116:555\u2013567","journal-title":"Parasitology"},{"key":"2557_CR5","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0065-308X(03)54004-0","volume":"54","author":"TH Cribb","year":"2003","unstructured":"Cribb TH, Bray RA, Olson PD, Littlewood DT (2003) Life cycle evolution in the digenea: a new perspective from phylogeny. Adv Parasitol 54:197\u2013254","journal-title":"Adv Parasitol"},{"key":"2557_CR6","first-page":"123","volume":"39","author":"O Ditrich","year":"1992","unstructured":"Ditrich O, Giboda M, Scholz T, Beer SA (1992) Comparative morphology of eggs of the Haplorchiinae (Trematoda: Heterophyidae) and some other medically important heterophyid and opisthorchiid flukes. Folia Parasitol (Praha) 39:123\u2013132","journal-title":"Folia Parasitol (Praha)"},{"key":"2557_CR7","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s00427-005-0467-z","volume":"215","author":"I Ebersberger","year":"2005","unstructured":"Ebersberger I, Knobloch J, Kunz W (2005) Cracks in the shell-zooming in on eggshell formation in the human parasite Schistosoma mansoni. Dev Genes Evol 215:261\u2013267","journal-title":"Dev Genes Evol"},{"key":"2557_CR8","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/0035-9203(90)90159-C","volume":"84","author":"DB Elkins","year":"1990","unstructured":"Elkins DB, Haswell-Elkins MR, Mairiang E, Mairiang P, Sithithaworn P, Kaewkes S, Bhudhisawasdi V, Uttaravichien T (1990) A high frequency of hepatobiliary disease and suspected cholangiocarcinoma associated with heavy Opisthorchis viverrini infection in a small community in north-east Thailand. Trans R Soc Trop Med Hyg 84:715\u2013719","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2557_CR9","first-page":"219","volume":"24","author":"SM El-Shoura","year":"1995","unstructured":"El-Shoura SM, Ramadan NF, Hassouna O, Mahmoud HI, Shetty S, Westmuckett A (1995) Human bilharzial ureters: I. Fine structure of eggs deposited in the submucosa and muscularis. Afr J Med Med Sci 24:219\u2013225","journal-title":"Afr J Med Med Sci"},{"key":"2557_CR10","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1096\/fj.06-7314com","volume":"21","author":"JM Fitzpatrick","year":"2007","unstructured":"Fitzpatrick JM, Hirai Y, Hirai H, Hoffmann KF (2007) Schistosome egg production is dependent upon the activities of two developmentally regulated tyrosinases. FASEB J 21:823\u2013835","journal-title":"FASEB J"},{"key":"2557_CR11","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s004360050689","volume":"86","author":"T Fujino","year":"2000","unstructured":"Fujino T, Nakano T, Washioka H, Tonosaki A, Ichikawa H, Fried B (2000) Comparative ultrastructure of eggs in Echinostoma paraensei, E. caproni, and E. trivolvis (Trematoda: Echinostomatidae). Parasitol Res 86:427\u2013430","journal-title":"Parasitol Res"},{"key":"2557_CR12","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0014-4894(72)90093-8","volume":"31","author":"SWB Irwin","year":"1971","unstructured":"Irwin SWB, Threadgold LT (1971) Electron microscope studied of Fasciola hepatica. X. Egg formation. Exp Parasitol 31:321\u2013331","journal-title":"Exp Parasitol"},{"key":"2557_CR13","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/S0020-7519(97)00007-6","volume":"27","author":"MK Jones","year":"1997","unstructured":"Jones MK, Ernst I, Whittington ID (1997) Variation in the egg cell forming region of Gyrodactylus kobayashii Hukuda, 1940 (Monogenea:Gyrodactylidae). Int J Parasitol 27:507\u2013516","journal-title":"Int J Parasitol"},{"key":"2557_CR14","doi-asserted-by":"crossref","first-page":"e334","DOI":"10.1371\/journal.pntd.0000334","volume":"2","author":"MK Jones","year":"2008","unstructured":"Jones MK, Bong SH, Green KM, Holmes P, Duke M, Loukas A, McManus DP (2008) Correlative and dynamic imaging of the hatching biology of Schistosoma japonicum from eggs prepared by high pressure freezing. PLoS Negl Trop Dis 2:e334","journal-title":"PLoS Negl Trop Dis"},{"key":"2557_CR15","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1017\/S003118200007726X","volume":"60","author":"JR Kusel","year":"1970","unstructured":"Kusel JR (1970) Studies on the structure and hatching of the eggs of Schistosoma mansoni. Parasitology 60:79\u201388","journal-title":"Parasitology"},{"key":"2557_CR16","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0020-7519(92)90081-U","volume":"22","author":"T Moczon","year":"1992","unstructured":"Moczon T, Swiderski Z, Huggel H (1992) Schistosoma mansoni: the chemical nature of the secretions produced by the Mehlis' gland and ootype as revealed by cytochemical studies. Int J Parasitol 22:65\u201373","journal-title":"Int J Parasitol"},{"key":"2557_CR17","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1007\/s00436-006-0321-x","volume":"100","author":"B M\u00fcller","year":"2007","unstructured":"M\u00fcller B, Schmidt J, Mehlhorn H (2007) PCR diagnosis of infections with different species of Opisthorchiidae using a rapid clean-up procedure for stool samples and specific primers. Parasitol Res 100:905\u2013909","journal-title":"Parasitol Res"},{"key":"2557_CR18","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4269\/ajtmh.1988.39.52","volume":"39","author":"PJ Neill","year":"1988","unstructured":"Neill PJ, Smith JH, Doughty BL, Kemp M (1988) The ultrastructure of the Schistosoma mansoni egg. Am J Trop Med Hyg 39:52\u201365","journal-title":"Am J Trop Med Hyg"},{"key":"2557_CR19","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/jmor.1052070103","volume":"207","author":"Y Orido","year":"1991","unstructured":"Orido Y (1991) Ultrastructure of Mehlis' gland in the lung fluke, Paragonimus ohirai (Trematoda: Troglotrematidae). J Morphol 207:9\u201316","journal-title":"J Morphol"},{"key":"2557_CR20","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/BF02351871","volume":"80","author":"Y Orido","year":"1994","unstructured":"Orido Y, Takamure A, Akamatsu T, Takashima Y (1994) Ultrastructure of the ootype of the lung fluke Paragonimus ohirai (Digenea, Troglotrematidae) in mated and single-infection worms. Parasitol Res 80:307\u2013311","journal-title":"Parasitol Res"},{"key":"2557_CR21","first-page":"405","volume":"16","author":"S Pungpak","year":"1985","unstructured":"Pungpak S, Riganti M, Bunnag D, Harinasuta T (1985) Clinical features in severe opisthorchiasis viverrini. Southeast Asian J Trop Med Public Health 16:405\u2013409","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"2557_CR22","first-page":"633","volume":"19","author":"M Riganti","year":"1988","unstructured":"Riganti M, Pungpak S, Sachakul V, Bunnag D, Harinasuta T (1988) Opisthorchis viverrini eggs and adult flukes as nidus and composition of gallstones. Southeast Asian J Trop Med Public Health 19:633\u2013636","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"2557_CR23","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s00436-003-0963-x","volume":"91","author":"HJ Rim","year":"2003","unstructured":"Rim HJ, Chai JY, Min DY, Cho SY, Eom KS, Hong SJ, Sohn WM, Yong TS, Deodato G, Standgaard H, Phommasack B, Yun CH, Hoang EH (2003) Prevalence of intestinal parasite infections on a national scale among primary schoolchildren in Laos. Parasitol Res 91:267\u2013272","journal-title":"Parasitol Res"},{"key":"2557_CR24","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1016\/j.ijpara.2006.06.012","volume":"36","author":"J Ruangsittichai","year":"2006","unstructured":"Ruangsittichai J, Viyanant V, Vichasri-Grams S, Sobhon P, Tesana S, Upatham ES, Hofmann A, Korge G, Grams R (2006) Opisthorchis viverrini: identification of a glycine-tyrosine rich eggshell protein and its potential as a diagnostic tool for human opisthorchiasis. Int J Parasitol 36:1329\u20131339","journal-title":"Int J Parasitol"},{"key":"2557_CR25","first-page":"81","volume":"62","author":"EH Sadun","year":"1955","unstructured":"Sadun EH (1955) Studies on Opisthorchis viverrini in Thailand. Am J Hyg 62:81\u2013115","journal-title":"Am J Hyg"},{"key":"2557_CR26","first-page":"60","volume":"5","author":"ChR Salikhova","year":"1989","unstructured":"Salikhova ChR, Liubarskaia OD, Beer SA (1989) The outer membranes of the eggs of Opisthorchis felineus and Clonorchis sinensis. Med Parazitol (Mosk) 5:60\u201362","journal-title":"Med Parazitol (Mosk)"},{"key":"2557_CR27","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/S0040-8166(98)80056-6","volume":"30","author":"J Schmidt","year":"1998","unstructured":"Schmidt J (1998) Glycan vesicle formation in vitellocytes and hatching vacuoles in eggs of Echinostoma caproni and Fasciola hepatica (Digenea). Tissue Cell 30:416\u2013426","journal-title":"Tissue Cell"},{"key":"2557_CR28","first-page":"125","volume":"75","author":"P Sithithaworn","year":"1997","unstructured":"Sithithaworn P, Pipitgool V, Srisawangwong T, Elkins DB, Haswell-Elkins MR (1997) Seasonal variation of Opisthorchis viverrini infection in cyprinoid fish in north-east Thailand: implications for parasite control and food safety. Bull World Health Organ 75:125\u2013131","journal-title":"Bull World Health Organ"},{"key":"2557_CR29","first-page":"446","volume-title":"The physiology of trematodes","author":"JD Smyth","year":"1983","unstructured":"Smyth JD, Halton DW (1983) The physiology of trematodes, 2nd edn. Cambridge University Press, Cambridge, p 446","edition":"2"},{"key":"2557_CR30","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1111\/j.1365-3156.2004.01234.x","volume":"9","author":"S Sriamporn","year":"2004","unstructured":"Sriamporn S, Pisani P, Pipitgool V, Suwanrungruang K, Kamsa-ard S, Parkin DM (2004) Prevalence of Opisthorchis viverrini infection and incidence of cholangiocarcinoma in Khon Kaen, Northeast Thailand. Trop Med Int Health 9:588\u2013594","journal-title":"Trop Med Int Health"},{"key":"2557_CR31","first-page":"653","volume":"36","author":"P Sri-Aroon","year":"2005","unstructured":"Sri-Aroon P, Butraporn P, Limsomboon J, Kerdpuech Y, Kaewpoolsri M, Kiatsiri S (2005) Freshwater mollusks of medical importance in Kalasin Province, northeast Thailand. Southeast Asian J Trop Med Public Health 36:653\u2013657","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"2557_CR32","doi-asserted-by":"crossref","first-page":"3318","DOI":"10.3748\/wjg.v10.i22.3318","volume":"10","author":"B Sripa","year":"2004","unstructured":"Sripa B, Kanla P, Sinawat P, Haswell-Elkins MR (2004) Opisthorchiasis-associated biliary stones: light and scanning electron microscopic study. World J Gastroenterol 10:3318\u20133321","journal-title":"World J Gastroenterol"},{"key":"2557_CR33","doi-asserted-by":"crossref","first-page":"e201","DOI":"10.1371\/journal.pmed.0040201","volume":"4","author":"B Sripa","year":"2007","unstructured":"Sripa B, Kaewkes S, Sithithaworn P, Mairiang E, Laha T, Smout M, Pairojkul C, Bhudhisawasdi V, Tesana S, Thinkamrop B, Bethony JM, Loukas A, Brindley PJ (2007) Liver fluke induces cholangiocarcinoma. PLoS Med 4:e201","journal-title":"PLoS Med"},{"key":"2557_CR34","first-page":"64","volume":"39","author":"Z \u015awiderski","year":"1994","unstructured":"\u015awiderski Z (1994) Origin, differentiation and ultrastructure of egg envelopes surrounding the miracidia of Schistosoma mansoni. Acta Parasitol 39:64\u201372","journal-title":"Acta Parasitol"},{"key":"2557_CR35","doi-asserted-by":"crossref","first-page":"245","DOI":"10.2478\/s11686-010-0031-5","volume":"55","author":"Z \u015awiderski","year":"2010","unstructured":"\u015awiderski Z, Bakhoum AJS, M\u0142ocicki D, Miquel J (2010) Ultrastructural studies on egg envelopes surrounding the miracidia of Mediogonimus jourdanei Mas-Coma et Rocamora, 1978 (Digenea, Microphalloidea, Prosthogonimidae). Acta Parasitol 55:245\u2013253","journal-title":"Acta Parasitol"},{"key":"2557_CR36","first-page":"631","volume":"22","author":"S Tesana","year":"1991","unstructured":"Tesana S, Srisawangwonk T, Kaewkes S, Sithithaworn P, Kanla P, Arunyanart C (1991) Eggshell morphology of the small eggs of human trematodes in Thailand. Southeast Asian J Trop Med Public Health 22:631\u2013636","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"2557_CR37","first-page":"489","volume":"21","author":"V Vatanasapt","year":"1990","unstructured":"Vatanasapt V, Tangvoraphonkchai V, Titapant V, Pipitgool V, Viriyapap D, Sriamporn S (1990) A high incidence of liver cancer in Khon Kaen Province, Thailand. Southeast Asian J Trop Med Public Health 21:489\u2013494","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"2557_CR38","first-page":"138","volume":"13","author":"S Vichasri","year":"1982","unstructured":"Vichasri S, Viyanant V, Upatham ES (1982) Opisthorchis viverrini: intensity and rates of infection in cyprinoid fish from an endemic focus in Northeast Thailand. Southeast Asian J Trop Med Public Health 13:138\u2013141","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"2557_CR39","doi-asserted-by":"crossref","first-page":"7819","DOI":"10.1021\/bi00398a043","volume":"26","author":"JH Waite","year":"1987","unstructured":"Waite JH, Rice-Ficht AC (1987) Presclerotized eggshell protein from the liver fluke Fasciola hepatica. Biochemistry 26:7819\u20137825","journal-title":"Biochemistry"},{"key":"2557_CR40","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0166-6851(92)90107-U","volume":"54","author":"JH Waite","year":"1992","unstructured":"Waite JH, Rice-Ficht AC (1992) Eggshell precursor proteins of Fasciola hepatica. II. Microheterogeneity in vitelline protein B. Mol Biochem Parasitol 54:143\u2013151","journal-title":"Mol Biochem Parasitol"},{"key":"2557_CR41","unstructured":"World Health Organization (2009) Review on the epidemiological profile of helminthiases and their control in the Western Pacific Region, 1997\u20132008"},{"issue":"2","key":"2557_CR42","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/0014-4894(90)90104-K","volume":"70","author":"YZ Xu","year":"1990","unstructured":"Xu YZ, Dresden MH (1990) The hatching of schistosome eggs. Exp Parasitol 70(2):236\u2013240","journal-title":"Exp Parasitol"},{"key":"2557_CR43","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0166-6851(89)90097-2","volume":"37","author":"M Zurita","year":"1989","unstructured":"Zurita M, Bieber D, Mansour TE (1989) Identification, expression and in situ hybridization of an eggshell protein gene from Fasciola hepatica. Mol Biochem Parasitol 37:11\u201317","journal-title":"Mol Biochem Parasitol"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-011-2557-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00436-011-2557-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-011-2557-3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,13]],"date-time":"2019-06-13T02:22:01Z","timestamp":1560392521000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00436-011-2557-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,23]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["2557"],"URL":"https:\/\/doi.org\/10.1007\/s00436-011-2557-3","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7,23]]}}}