{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:37:18Z","timestamp":1792175838606,"version":"4.4.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T00:00:00Z","timestamp":1326412800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2012,7]]},"DOI":"10.1007\/s00436-011-2804-7","type":"journal-article","created":{"date-parts":[[2012,1,16]],"date-time":"2012-01-16T06:02:43Z","timestamp":1326693763000},"page":"89-96","source":"Crossref","is-referenced-by-count":35,"title":["Rapid detection and differentiation of Clonorchis sinensis and Opisthorchis viverrini eggs in human fecal samples using a duplex real-time fluorescence resonance energy transfer PCR and melting curve analysis"],"prefix":"10.1007","volume":"111","author":[{"given":"Oranuch","family":"Sanpool","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penchom","family":"Janwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viraphong","family":"Lulitanond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pham Ngoc","family":"Doanh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoang","family":"Van Hien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Trung","family":"Dung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukifumi","family":"Nawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,1,13]]},"reference":[{"key":"2804_CR1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.parint.2011.06.010","volume":"61","author":"XQ Cai","year":"2012","unstructured":"Cai XQ, Yu HQ, Bai JS, Tang JD, Hu XC, Chen DH, Zhang RL, Chen MX, Ai L, Zhu XQ (2012) Development of a TaqMan based real-time PCR assay for detection of Clonorchis sinensis DNA in human stool samples and fishes. Parasitol Int 61:183\u2013186","journal-title":"Parasitol Int"},{"key":"2804_CR2","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1007\/s00436-008-1086-1","volume":"103","author":"Y Caron","year":"2008","unstructured":"Caron Y, Rondelaud D, Losson B (2008) The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp. Parasitol Res 103:735\u2013744","journal-title":"Parasitol Res"},{"key":"2804_CR3","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1469-0691.2010.03218.x","volume":"17","author":"L Dormond","year":"2011","unstructured":"Dormond L, Jaton-Ogay K, de Valli\u00e8re S, Genton B, Bille J, Greub G (2011) Multiplex real-time PCR for the diagnosis of malaria: correlation with microscopy. Clin Microbiol Infect 17:469\u2013475","journal-title":"Clin Microbiol Infect"},{"key":"2804_CR4","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.vetpar.2009.05.026","volume":"163","author":"P Dorny","year":"2009","unstructured":"Dorny P, Praet N, Deckers N, Gabriel S (2009) Emerging food-borne parasites. Vet Parasitol 163:196\u2013206","journal-title":"Vet Parasitol"},{"key":"2804_CR5","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/0035-9203(86)90384-6","volume":"80","author":"DB Elkins","year":"1986","unstructured":"Elkins DB, Haswell-Elkins M, Anderson RM (1986) The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Trans R Soc Trop Med Hyg 80:774\u2013792","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2804_CR6","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1007\/s00436-010-1807-0","volume":"106","author":"B Fried","year":"2010","unstructured":"Fried B, Abruzzi A (2010) Food-borne trematode infections of humans in the United States of America. Parasitol Res 106:1263\u20131280","journal-title":"Parasitol Res"},{"key":"2804_CR7","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.canlet.2010.07.008","volume":"305","author":"B Fried","year":"2011","unstructured":"Fried B, Reddy A, Mayer D (2011) Helminths in human carcinogenesis. Cancer Lett 305:239\u2013249","journal-title":"Cancer Lett"},{"key":"2804_CR8","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/S0031-3955(16)33930-X","volume":"27","author":"RS Galen","year":"1980","unstructured":"Galen RS (1980) Predictive value and efficiency of laboratory testing. Pediatr Clin North Am 27:861\u2013869","journal-title":"Pediatr Clin North Am"},{"key":"2804_CR9","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.parint.2011.06.007","volume":"61","author":"ST Hong","year":"2012","unstructured":"Hong ST, Fang Y (2012) Clonorchis sinensis and clonorchiasis, an update. Parasitol Int 61:17\u201324","journal-title":"Parasitol Int"},{"key":"2804_CR10","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.actatropica.2011.05.006","volume":"120","author":"SY Hwang","year":"2011","unstructured":"Hwang SY, Kim SH, Lee GY, Hang VT, Moon CS, Shin JH, Koo WL, Kim SY, Park HJ, Park HO, Kho WG (2011) A novel real-time PCR assay for the detection of Plasmodium falciparum and Plasmodium vivax malaria in low parasitized individuals. Acta Trop 120:40\u201345","journal-title":"Acta Trop"},{"key":"2804_CR11","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1007\/s00436-008-1026-0","volume":"103","author":"PM Intapan","year":"2008","unstructured":"Intapan PM, Thanchomnang T, Lulitanond V, Pongsaskulchoti P, Maleewong W (2008a) Detection of Opisthorchis viverrini in infected bithynid snails by real-time fluorescence resonance energy transfer PCR-based method and melting curve analysis. Parasitol Res 103:649\u2013655","journal-title":"Parasitol Res"},{"key":"2804_CR12","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.vetpar.2008.07.012","volume":"157","author":"PM Intapan","year":"2008","unstructured":"Intapan PM, Thanchomnang T, Lulitanond V, Phongsaskulchoti P, Maleewong W (2008b) Real-time fluorescence resonance energy transfer PCR with melting curve analysis for the detection of Opisthorchis viverrini in fish intermediate hosts. Vet Parasitol 157:65\u201371","journal-title":"Vet Parasitol"},{"key":"2804_CR13","doi-asserted-by":"crossref","first-page":"917","DOI":"10.4269\/ajtmh.2009.09-0275","volume":"81","author":"PM Intapan","year":"2009","unstructured":"Intapan PM, Thanchomnang T, Lulitanond V, Pongsaskulchoti P, Maleewong W (2009a) Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction. AmJTrop Med Hyg 81:917\u2013920","journal-title":"AmJTrop Med Hyg"},{"key":"2804_CR14","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1603\/033.046.0119","volume":"46","author":"PM Intapan","year":"2009","unstructured":"Intapan PM, Thanchomnang T, Lulitanond V, Maleewong W (2009b) Rapid detection of Wuchereria bancrofti and Brugia malayi in mosquito vectors (Diptera: Culicidae) using a real-time fluorescence resonance energy transfer multiplex PCR and melting curve analysis. J Med Entomol 46:158\u2013164","journal-title":"J Med Entomol"},{"key":"2804_CR15","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1007\/s00436-011-2419-z","volume":"109","author":"P Janwan","year":"2011","unstructured":"Janwan P, Intapan PM, Thanchomnang T, Lulitanond V, Anamnart W, Maleewong W (2011) Rapid detection of Opisthorchis viverrini and Strongyloides stercoralis in human fecal samples using a duplex real-time PCR and melting curve analysis. Parasitol Res 109:1593\u20131601","journal-title":"Parasitol Res"},{"key":"2804_CR16","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.parint.2007.12.007","volume":"57","author":"S Kang","year":"2008","unstructured":"Kang S, Sultana T, Loktev VB, Wongratanacheewin S, Sohn WM, Eom KS, Park JK (2008) Molecular identification and phylogenetic analysis of nuclear rDNA sequences among three opisthorchid liver fluke species (Opisthorchiidae: Trematoda). Parasitol Int 57:191\u2013197","journal-title":"Parasitol Int"},{"key":"2804_CR17","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.pt.2007.07.012","volume":"23","author":"J Keiser","year":"2007","unstructured":"Keiser J, Utzinger J (2007) Food-borne trematodiasis: current chemotherapy and advances with artemisinins and synthetic trioxolanes. Trends Parasitol 23:555\u2013562","journal-title":"Trends Parasitol"},{"key":"2804_CR18","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1128\/CMR.00012-09","volume":"22","author":"J Keiser","year":"2009","unstructured":"Keiser J, Utzinger J (2009) Food-borne trematodiases. Clin Microbiol Rev 22:466\u2013483","journal-title":"Clin Microbiol Rev"},{"key":"2804_CR19","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1186\/1475-2875-8-284","volume":"8","author":"K Khairnar","year":"2009","unstructured":"Khairnar K, Martin D, Lau R, Ralevski F, Pilla IDR (2009) Multiplex real-time quantitative PCR, microscopy and rapid diagnostic immuno-chromatographic tests for the detection of Plasmodium spp performance, limit of detection analysis and quality assurance. Malar J 8:284","journal-title":"Malar J"},{"key":"2804_CR20","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1179\/136485909X451834","volume":"103","author":"EM Kim","year":"2009","unstructured":"Kim EM, Verweij JJ, Jalili A, van Lieshout L, Choi MH, Bae YM, Lim MK, Hong ST (2009) Detection of Clonorchis sinensis in stool samples using real-time PCR. Ann Trop Med Parasitol 103:513\u2013518","journal-title":"Ann Trop Med Parasitol"},{"key":"2804_CR21","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.exppara.2005.09.012","volume":"112","author":"TH Le","year":"2006","unstructured":"Le TH, Van De N, Blair D, Sithithaworn P, McManus DP (2006) Clonorchis sinensis and Opisthorchis viverrini: development of a mitochondrial-based multiplex PCR for their identification and discrimination. Exp Parasitol 112:109\u2013114","journal-title":"Exp Parasitol"},{"key":"2804_CR22","doi-asserted-by":"crossref","first-page":"93","DOI":"10.2353\/jmoldx.2009.080094","volume":"11","author":"E Lyon","year":"2009","unstructured":"Lyon E, Wittwer CT (2009) LightCycler technology in molecular diagnostics. J Mol Diagn 11:93\u2013101","journal-title":"J Mol Diagn"},{"key":"2804_CR23","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1097\/QCO.0b013e32833df718","volume":"23","author":"M Montes","year":"2010","unstructured":"Montes M, Sawhney C, Barros N (2010) Strongyloides stercoralis: there but not seen. Curr Opin Infect Dis 23:500\u2013504","journal-title":"Curr Opin Infect Dis"},{"key":"2804_CR24","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s00436-006-0269-x","volume":"100","author":"GM Park","year":"2007","unstructured":"Park GM (2007) Genetic comparison of liver flukes, Clonorchis sinensis and Opisthorchis viverrini, based on rDNA and mtDNA gene sequences. Parasitol Res 100:351\u2013357","journal-title":"Parasitol Res"},{"key":"2804_CR25","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s00436-011-2253-3","volume":"109","author":"SM Rahman","year":"2011","unstructured":"Rahman SM, Bae YM, Hong ST, Choi MH (2011) Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis. Parasitol Res 109:297\u2013303","journal-title":"Parasitol Res"},{"key":"2804_CR26","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.actatropica.2008.09.015","volume":"109","author":"M Sato","year":"2009","unstructured":"Sato M, Thaenkham U, Dekumyoy P, Waikagul J (2009) Discrimination of O. viverrini, C. sinensis, H. pumilio and H. taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions. Acta Trop 109:81\u201383","journal-title":"Acta Trop"},{"key":"2804_CR27","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s00436-009-1628-1","volume":"106","author":"SV Shekhovtsov","year":"2009","unstructured":"Shekhovtsov SV, Katokhin AV, Romanov KV, Besprozvannykh VV, Fedorov KP, Yurlova NI, Serbina EA, Sithithaworn P, Kolchanov NA, Mordvinov VA (2009) A novel nuclear marker, Pm-int9, for phylogenetic studies of Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis (Opisthorchiidae, Trematoda). Parasitol Res 106:293\u2013297","journal-title":"Parasitol Res"},{"key":"2804_CR28","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1007\/s00436-010-2140-3","volume":"108","author":"P Sri-Aroon","year":"2011","unstructured":"Sri-Aroon P, Intapan PM, Lohachit C, Phongsasakulchoti P, Thanchomnang T, Lulitanond V, Hiscox A, Phompida S, Sananikhom P, Maleewong W, Brey PT (2011) Molecular evidence of Opisthorchis viverrini in infected bithyniid snails in the Lao People\u2019s Democratic Republic by specific hybridization probe-based real-time fluorescence resonance energy transfer PCR method. Parasitol Res 108:973\u2013978","journal-title":"Parasitol Res"},{"key":"2804_CR29","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0065-308X(10)72011-X","volume":"72","author":"B Sripa","year":"2010","unstructured":"Sripa B, Kaewkes S, Intapan PM, Maleewong W, Brindley PJ (2010) Food-borne trematodiases in Southeast Asia epidemiology, pathology, clinical manifestation and control. Adv Parasitol 72:305\u2013350","journal-title":"Adv Parasitol"},{"key":"2804_CR30","first-page":"157","volume":"11","author":"WM Stauffer","year":"2004","unstructured":"Stauffer WM, Sellman JS, Walker PF (2004) Biliary liver flukes (opisthorchiasis and clonorchiasis) in immigrants in the United States: often subtle and diagnosed years after arrival. J Travel Med 11:157\u2013159","journal-title":"J Travel Med"},{"key":"2804_CR31","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.exppara.2008.01.018","volume":"119","author":"N Suksumek","year":"2008","unstructured":"Suksumek N, Leelawat K, Leelawat S, Russell B, Lek-Uthai U (2008) TaqMan real-time PCR assay for specific detection of Opisthorchis viverrini DNA in Thai patients with hepatocellular carcinoma and cholangiocarcinoma. Exp Parasitol 119:217\u2013224","journal-title":"Exp Parasitol"},{"key":"2804_CR32","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/1756-3305-4-98","volume":"4","author":"J Sun","year":"2011","unstructured":"Sun J, Xu J, Liang P, Mao Q, Huang Y, Lv X, Deng C, Liang C, de Hoog GS, Yu X (2011) Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA). Parasit Vectors 4:98","journal-title":"Parasit Vectors"},{"key":"2804_CR33","doi-asserted-by":"crossref","first-page":"332","DOI":"10.4269\/ajtmh.2011.10-0461","volume":"84","author":"M Taniuchi","year":"2011","unstructured":"Taniuchi M, Verweij JJ, Noor Z, Sobuz SU, Lieshout L, Petri WA Jr, Haque R, Houpt ER (2011) High throughput multiplex PCR and probe-based detection with Luminex beads for seven intestinal parasites. AmJTrop Med Hyg 84:332\u2013337","journal-title":"AmJTrop Med Hyg"},{"key":"2804_CR34","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1111\/j.1469-0691.2007.01788.x","volume":"13","author":"R Hove ten","year":"2007","unstructured":"ten Hove R, Schuurman T, Kooistra M, M\u00f6ller L, van Lieshout L, Verweij JJ (2007) Detection of diarrhoea-causing protozoa in general practice patients in the Netherlands by multiplex real-time PCR. Clin Microbiol Infect 13:1001\u20131007","journal-title":"Clin Microbiol Infect"},{"key":"2804_CR35","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.trstmh.2007.10.011","volume":"102","author":"RJ Hove ten","year":"2008","unstructured":"ten Hove RJ, Verweij JJ, Vereecken K, Polman K, Dieye L, van Lieshout L (2008) Multiplex real-time PCR for the detection and quantification of Schistosoma mansoni and S. haematobium infection in stool samples collected in northern Senegal. Trans R Soc Trop Med Hyg 102:179\u2013185","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2804_CR36","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1007\/s10096-009-0745-1","volume":"28","author":"RJ Hove ten","year":"2009","unstructured":"ten Hove RJ, van Esbroeck M, Vervoort T, van den Ende J, van Lieshout L, Verwei JJ (2009) Molecular diagnostics of intestinal parasites in returning travellers. Eur J Clin Microbiol Infect Dis 28:1045\u20131053","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2804_CR37","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1371\/journal.pntd.0000367","volume":"3","author":"RJ Traub","year":"2009","unstructured":"Traub RJ, Macaranas J, Mungthin M, Leelayoova S, Cribb T, Murrell KD, Thompson RC (2009) A new PCR-based approach indicates the range of Clonorchis sinensis now extends to Central Thailand. PLoS Negl Trop Dis 3:367","journal-title":"PLoS Negl Trop Dis"},{"key":"2804_CR38","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1097\/QCO.0b013e32833de0eb","volume":"23","author":"L Lieshout van","year":"2010","unstructured":"van Lieshout L, Verweij JJ (2010) Newer diagnostic approaches to intestinal protozoa. Curr Opin Infect Dis 23:488\u2013493","journal-title":"Curr Opin Infect Dis"},{"key":"2804_CR39","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.exppara.2008.12.012","volume":"121","author":"V Veron","year":"2009","unstructured":"Veron V, Simon S, Carme B (2009) Multiplex real-time PCR detection of P. falciparum, P. vivax and P. malariae in human blood samples. Exp Parasitol 121:346\u2013351","journal-title":"Exp Parasitol"},{"key":"2804_CR40","doi-asserted-by":"crossref","first-page":"685","DOI":"10.4269\/ajtmh.2007.77.685","volume":"77","author":"JJ Verweij","year":"2007","unstructured":"Verweij JJ, Brienen EA, Ziem J, Yelifari L, Polderman AM, Van Lieshout L (2007) Simultaneous detection and quantification of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in fecal samples using multiplex real-time PCR. AmJTrop Med Hyg 77:685\u2013690","journal-title":"AmJTrop Med Hyg"},{"key":"2804_CR41","first-page":"1","volume":"849","author":"World Health Organization","year":"1995","unstructured":"World Health Organization (1995) Control of foodborne trematode infections. Report of a WHO Study Group. World Health Organ Tech Rep Ser 849:1\u2013157","journal-title":"World Health Organ Tech Rep Ser"},{"key":"2804_CR42","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.3201\/eid1012.040410","volume":"10","author":"O Yossepowitch","year":"2004","unstructured":"Yossepowitch O, Gotesman T, Assous M, Marva E, Zimlichman R, Dan M (2004) Opisthorchiasis from imported raw fish. Emerg Infect Dis 10:2122\u20132126","journal-title":"Emerg Infect Dis"},{"key":"2804_CR43","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0304-4017(01)00568-4","volume":"101","author":"DS Zarlenga","year":"2001","unstructured":"Zarlenga DS, Higgins J (2001) PCR as a diagnostic and quantitative technique in veterinary parasitology. Vet Parasitol 101:215\u2013230","journal-title":"Vet Parasitol"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-011-2804-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00436-011-2804-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-011-2804-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,22]],"date-time":"2019-06-22T10:35:40Z","timestamp":1561199740000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00436-011-2804-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,13]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["2804"],"URL":"https:\/\/doi.org\/10.1007\/s00436-011-2804-7","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,13]]}}}