{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:21:28Z","timestamp":1764688888338},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2012,8,11]],"date-time":"2012-08-11T00:00:00Z","timestamp":1344643200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2012,11]]},"DOI":"10.1007\/s00436-012-3064-x","type":"journal-article","created":{"date-parts":[[2012,8,10]],"date-time":"2012-08-10T07:53:23Z","timestamp":1344585203000},"page":"2157-2163","source":"Crossref","is-referenced-by-count":27,"title":["Application of HRM assays with EvaGreen dye for genotyping Giardia duodenalis zoonotic assemblages"],"prefix":"10.1007","volume":"111","author":[{"given":"Ping","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanjia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhamd","family":"Alsarakibi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yawen","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoqing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,8,11]]},"reference":[{"issue":"1","key":"3064_CR1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.4269\/ajtmh.2009.80.16","volume":"80","author":"SS Ajjampur","year":"2009","unstructured":"Ajjampur SS, Sankaran P, Kannan A, Sathyakumar K, Sarkar R, Gladstone BP, Kang G (2009) Giardia duodenalis assemblages associated with diarrhea in children in South India identified by PCR-RFLP. Am J Trop Med Hyg 80(1):16\u201319","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"3064_CR2","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1128\/AEM.02305-09","volume":"76","author":"A Almeida","year":"2010","unstructured":"Almeida A, Pozio E, Cacci\u00f2 SM (2010) Genotyping of Giardia duodenalis cysts by new real-time PCR assays for detection of mixed infections in human samples. Appl Environ Microbiol 76(6):1895\u20131901","journal-title":"Appl Environ Microbiol"},{"key":"3064_CR3","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1007\/s00436-010-2033-5","volume":"108","author":"HI Al-Mohammed","year":"2011","unstructured":"Al-Mohammed HI (2011) Genotypes of Giardia intestinalis clinical isolates of gastrointestinal symptomatic and asymptomatic Saudi children. Parasitol Res 108:1375\u20131381","journal-title":"Parasitol Res"},{"key":"3064_CR4","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1128\/JCM.40.2.446-452.2002","volume":"40","author":"CF Amar","year":"2002","unstructured":"Amar CF, Dear PH, Pedraza-Diaz S, Looker N, Linnane E, McLauchlin J (2002) Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces. J Clin Microbiol 40:446\u2013452","journal-title":"J Clin Microbiol"},{"key":"3064_CR5","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1099\/jmm.0.05193-0","volume":"52","author":"CF Amar","year":"2003","unstructured":"Amar CF, Dear PH, McLauchlin J (2003) Detection and genotyping by real-time PCR\/RFLP analyses of Giardia duodenalis from human faeces. J Med Microbiol 52:681\u2013683","journal-title":"J Med Microbiol"},{"key":"3064_CR6","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.vetpar.2004.04.008","volume":"122","author":"F Berrilli","year":"2004","unstructured":"Berrilli F, Di Cave D, De Liberato C, Franco A, Scaramozzino P, Orecchia P (2004) Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing. Vet Parasitol 122:193\u2013199","journal-title":"Vet Parasitol"},{"key":"3064_CR7","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1186\/1756-3305-4-199","volume":"4","author":"F Berrilli","year":"2011","unstructured":"Berrilli F, Prisco C, Friedrich KG, Di Cerbo P, Di Cavel D, De Liberato C (2011) Giardia duodenalis assemblages and Entamoeba species infecting non-human primates in an Italian zoological garden: zoonotic potential and management traits. Parasites Vectors 4:199","journal-title":"Parasites Vectors"},{"key":"3064_CR8","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.molbiopara.2008.04.006","volume":"160","author":"SM Cacci\u00f2","year":"2008","unstructured":"Cacci\u00f2 SM, Ryan U (2008) Molecular epidemiology of giardiasis. Mol Biochem Parasitol 160:75\u201380","journal-title":"Mol Biochem Parasitol"},{"key":"3064_CR9","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.pt.2005.06.013","volume":"21","author":"SM Cacci\u00f2","year":"2005","unstructured":"Cacci\u00f2 SM, Thompson RCA, McLauchlin J, Smith HV (2005) Unravelling Cryptosporidium and Giardia epidemiology. Trends Parasitol 21:430\u2013437","journal-title":"Trends Parasitol"},{"key":"3064_CR10","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.vetpar.2008.04.029","volume":"156","author":"L Eligio-Garcia","year":"2008","unstructured":"Eligio-Garcia L, Cortes-Campos A, Cota-Guajardo S, Gaxiola S, Jimenez-Cardoso E (2008a) Frequency of Giardia intestinalis assemblages isolated from dogs and humans in a community from Culiacan, Sinaloa, Mexico using beta-giardin restriction gene. Vet Parasitol 156:205\u2013209","journal-title":"Vet Parasitol"},{"key":"3064_CR11","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1007\/s00436-008-1042-0","volume":"103","author":"L Eligio-Garcia","year":"2008","unstructured":"Eligio-Garcia L, Cortes-Campos A, Jimenez-Cardoso E (2008b) Classification of Giardia intestinalis isolates by multiple polymerase chain reaction (multiplex). Parasitol Res 103:797\u2013800","journal-title":"Parasitol Res"},{"key":"3064_CR12","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.yexmp.2008.03.012","volume":"85","author":"M Erali","year":"2008","unstructured":"Erali M, Voelkerding KV, Witter CT (2008) High resolution melting applications for clinical laboratory medicine. Exp Mol Pathol 85:50\u201358","journal-title":"Exp Mol Pathol"},{"issue":"1","key":"3064_CR13","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1128\/CMR.00033-10","volume":"24","author":"Y Feng","year":"2011","unstructured":"Feng Y, Xiao L (2011) Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev 24(1):110\u2013140","journal-title":"Clin Microbiol Rev"},{"issue":"7","key":"3064_CR14","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1128\/JCM.42.7.3317-3320.2004","volume":"42","author":"RA Guy","year":"2004","unstructured":"Guy RA, Xiao C, Horgen PA (2004) Real-Time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens. J Clin Microbiol 42(7):3317\u20133320","journal-title":"J Clin Microbiol"},{"key":"3064_CR15","doi-asserted-by":"crossref","first-page":"713","DOI":"10.4269\/ajtmh.2007.76.713","volume":"76","author":"R Haque","year":"2007","unstructured":"Haque R, Roy S, Siddique A, Mondal U, Rahman SM, Mondal D, Houpt E, Petry WA Jr (2007) Multiplex real-time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. Am J Trop Med Hyg 76:713\u2013717","journal-title":"Am J Trop Med Hyg"},{"key":"3064_CR16","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ijpara.2004.10.022","volume":"35","author":"M Lalle","year":"2005","unstructured":"Lalle M, Pozio E, Capelli G, Bruschi F, Crotti D, Cacci\u00f2 SM (2005) Genetic heterogeneity at the beta-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes. Int J Parasitol 35:207\u2013213","journal-title":"Int J Parasitol"},{"issue":"1","key":"3064_CR17","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.actatropica.2008.01.004","volume":"106","author":"M Lebbad","year":"2008","unstructured":"Lebbad M, Ankarklev J, Tellez A, Leiva B, Andersson JO, Sv\u00e4rd S (2008) Dominance of Giardia assemblage B in Le\u00f3n, Nicaragua. Acta Trop 106(1):44\u201353","journal-title":"Acta Trop"},{"key":"3064_CR18","doi-asserted-by":"crossref","unstructured":"Li J, Zhang P, Wang P, Alsarakibi M, Zhu H, Liu Y, Meng X, Li J, Guo J, Li G (2012) Genotype identification and prevalence of Giardia duodenalis in pet dogs of Guangzhou, Southern China. Vet Parasitol. Available from doi: 10.1016\/j.vetpar.2012.04.004","DOI":"10.1016\/j.vetpar.2012.04.004"},{"key":"3064_CR19","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/1472-6750-7-76","volume":"7","author":"F Mao","year":"2007","unstructured":"Mao F, Leung WY, Xin X (2007) Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications. BMC Biotechnol 7:76","journal-title":"BMC Biotechnol"},{"issue":"2","key":"3064_CR20","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1128\/AEM.01164-09","volume":"76","author":"S Merchant-Patel","year":"2010","unstructured":"Merchant-Patel S, Blackall PJ, Templeton J, Price EP, Tong SY, Huygens F, Giffard PM (2010) Campylobacter jejuni and Campylobacter coli genotyping by high-resolution melting analysis of a flaA Fragment. Appl Environ Microbiol 76(2):493\u2013499","journal-title":"Appl Environ Microbiol"},{"key":"3064_CR21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182000065021","volume":"112","author":"PT Monis","year":"1996","unstructured":"Monis PT, Mayrhofer G, Andrews RH, Homan WL, Limper L, Ey PL (1996) Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus. Parasitology 112:1\u201312","journal-title":"Parasitology"},{"key":"3064_CR22","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1093\/oxfordjournals.molbev.a026204","volume":"16","author":"PT Monis","year":"1999","unstructured":"Monis PT, Andrews RH, Mayrhofer G, Ey PL (1999) Molecular systematics of the parasitic protozoan Giardia intestinalis. Mol Biol Evol 16:1135\u20131144","journal-title":"Mol Biol Evol"},{"key":"3064_CR23","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1006\/expr.1994.1008","volume":"78","author":"MR Mowatt","year":"1994","unstructured":"Mowatt MR, Weinbach EC, Howard TC, Nash TT (1994) Complementation of Escherichia coli glycolysis mutant by Giardia lamblia triosephosphate isomerase. Exp Parasitol 78:85\u201392","journal-title":"Exp Parasitol"},{"key":"3064_CR24","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0020-7519(01)00340-X","volume":"32","author":"C Read","year":"2002","unstructured":"Read C, Walters J, Robertson ID, Thompson RC (2002) Correlation between genotype of Giardia duodenalis and diarrhoea. Int J Parasitol 32:229\u2013231","journal-title":"Int J Parasitol"},{"key":"3064_CR25","doi-asserted-by":"crossref","first-page":"597","DOI":"10.2217\/14622416.8.6.597","volume":"8","author":"G Reed","year":"2007","unstructured":"Reed G, Kent J, Walters C (2007) High resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics 8:597\u2013608","journal-title":"Pharmacogenomics"},{"issue":"7","key":"3064_CR26","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1016\/j.ijpara.2006.03.001","volume":"36","author":"JW Giessen Van der","year":"2006","unstructured":"Van der Giessen JW, de Vries A, Roos M, Wielinqa P, Kortbeek LM, Mank TG (2006) Genotyping of Giardia in Dutch patients and animals: a phylogenetic analysis of human and animal isolates. Int J Parasitol 36(7):849\u2013858","journal-title":"Int J Parasitol"},{"key":"3064_CR27","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0166-6851(91)90051-7","volume":"46","author":"H Keulen Van","year":"1991","unstructured":"Van Keulen H, Campbell SR, Erlandsen SL, Jarroll EL (1991) Cloning and restriction enzyme mapping of ribosomal DNA of Giardia duodenalis, Giardia ardeae and Giardia muris. Mol Biochem Parasitol 46:275\u2013284","journal-title":"Mol Biochem Parasitol"},{"key":"3064_CR28","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0022-1759(03)00223-0","volume":"278","author":"JA Whelan","year":"2003","unstructured":"Whelan JA, Russel NB, Whelan MA (2003) A method for the absolute quantification of cDNA using real time PCR. J Immunol Meth 278:261\u2013269","journal-title":"J Immunol Meth"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-012-3064-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00436-012-3064-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-012-3064-x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T22:59:14Z","timestamp":1559084354000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00436-012-3064-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,11]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["3064"],"URL":"https:\/\/doi.org\/10.1007\/s00436-012-3064-x","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,11]]}}}