{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T01:07:04Z","timestamp":1775092024396,"version":"3.50.1"},"reference-count":63,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100010292","name":"Fundaci\u00f3n Miguel Alem\u00e1n, A.C.","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010292","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008688","name":"Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008688","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["296155"],"award-info":[{"award-number":["296155"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["295997"],"award-info":[{"award-number":["295997"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1007\/s00436-018-6163-5","type":"journal-article","created":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T16:26:04Z","timestamp":1543681564000},"page":"289-306","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of metalloproteases and serine proteases of Toxoplasma gondii tachyzoites and their effect on epithelial cells"],"prefix":"10.1007","volume":"118","author":[{"given":"Carlos J.","family":"Ram\u00edrez-Flores","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosalba","family":"Cruz-Mir\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossana","family":"Arroyo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica E.","family":"Mondrag\u00f3n-Castel\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tais","family":"Nopal-Guerrero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirenia","family":"Gonz\u00e1lez-Pozos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"R\u00edos-Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1913-9945","authenticated-orcid":false,"given":"Ricardo","family":"Mondrag\u00f3n-Flores","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,12,1]]},"reference":[{"key":"6163_CR1","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1006\/bbrc.2001.5637","volume":"287","author":"HJ Ahn","year":"2001","unstructured":"Ahn HJ, Song KJ, Son ES, Shin JC, Nam HW (2001) Protease activity and host cell binding of the 42-kDa rhoptry protein from Toxoplasma gondii after secretion. Biochem Biophys Res Commun 287:630\u2013635","journal-title":"Biochem Biophys Res Commun"},{"key":"6163_CR2","doi-asserted-by":"publisher","first-page":"3387","DOI":"10.1093\/bioinformatics\/btx431","volume":"33","author":"JJ Almagro Armenteros","year":"2017","unstructured":"Almagro Armenteros JJ, Sonderby CK, Sonderby SK, Nielsen H, Winther O (2017) DeepLoc: prediction of protein subcellular localization using deep learning. Bioinformatics 33:3387\u20133395","journal-title":"Bioinformatics"},{"key":"6163_CR3","doi-asserted-by":"publisher","first-page":"1625","DOI":"10.1084\/jem.20020258","volume":"195","author":"A Barrag\u00e1n","year":"2002","unstructured":"Barrag\u00e1n A, Sibley LD (2002) Transepithelial migration of Toxoplasma gondii is linked to parasite motility and virulence. J Exp Med 195:1625\u20131633","journal-title":"J Exp Med"},{"key":"6163_CR4","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1016\/S0966-842X(03)00205-1","volume":"11","author":"A Barrag\u00e1n","year":"2003","unstructured":"Barrag\u00e1n A, Sibley LD (2003) Migration of Toxoplasma gondii across biological barriers. Trends Microbiol 11:426\u2013430","journal-title":"Trends Microbiol"},{"key":"6163_CR5","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1111\/j.1462-5822.2005.00486.x","volume":"7","author":"A Barrag\u00e1n","year":"2005","unstructured":"Barrag\u00e1n A, Brossier F, Sibley LD (2005) Transepithelial migration of Toxoplasma gondii involves an interaction of intercellular adhesion molecule 1 (ICAM-1) with the parasite adhesin MIC2. Cell Microbiol 7:561\u2013568","journal-title":"Cell Microbiol"},{"key":"6163_CR6","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1006\/expr.2000.4524","volume":"95","author":"J Berthonneau","year":"2000","unstructured":"Berthonneau J, Rodier MH, El Moudni B, Jacquemin JL (2000) Toxoplasma gondii: purification and characterization of an immunogenic metallopeptidase. Exp Parasitol 95:158\u2013162","journal-title":"Exp Parasitol"},{"key":"6163_CR7","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1016\/j.bbrc.2007.05.089","volume":"359","author":"E Buache","year":"2007","unstructured":"Buache E, Garnotel R, Aubert D, Gillery P, Villena I (2007) Reduced secretion and expression of gelatinase profile in Toxoplasma gondii-infected human monocytic cells. Biochem Biophys Res Commun 359:298\u2013303","journal-title":"Biochem Biophys Res Commun"},{"key":"6163_CR8","doi-asserted-by":"publisher","first-page":"e1000858","DOI":"10.1371\/journal.ppat.1000858","volume":"6","author":"JS Buguliskis","year":"2010","unstructured":"Buguliskis JS, Brossier F, Shuman J, Sibley LD (2010) Rhomboid 4 (ROM4) affects the processing of surface adhesins and facilitates host cell invasion by Toxoplasma gondii. PLoS Pathog 6:e1000858","journal-title":"PLoS Pathog"},{"key":"6163_CR9","doi-asserted-by":"publisher","first-page":"e0193982","DOI":"10.1371\/journal.pone.0193982","volume":"13","author":"JD Chaparro","year":"2018","unstructured":"Chaparro JD, Cheng T, Tran UP, Andrade RM, Brenner SBT, Hwang G, Cohn S, Hirata K, McKerrow JH, Reed SL (2018) Two key cathepsins, TgCPB and TgCPL, are targeted by the vinyl sulfone inhibitor K11777 in in vitro and in vivo models of toxoplasmosis. PLoS One 13:e0193982","journal-title":"PLoS One"},{"key":"6163_CR10","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1016\/j.tcb.2015.01.004","volume":"25","author":"E Cocucci","year":"2015","unstructured":"Cocucci E, Meldolesi J (2015) Ectosomes and exosomes: shedding the confusion between extracellular vesicles. Trends Cell Biol 25:364\u2013372","journal-title":"Trends Cell Biol"},{"key":"6163_CR11","doi-asserted-by":"publisher","first-page":"8409183","DOI":"10.1155\/2016\/8409183","volume":"2016","author":"DF Coelho","year":"2016","unstructured":"Coelho DF, Saturnino TP, Fernandes FF, Mazzola PG, Silveira E, Tambourgi EB (2016) Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples. Biomed Res Int 2016:8409183","journal-title":"Biomed Res Int"},{"key":"6163_CR12","doi-asserted-by":"publisher","first-page":"e10809","DOI":"10.7554\/eLife.10809","volume":"4","author":"MJ Coffey","year":"2015","unstructured":"Coffey MJ, Sleebs BE, Uboldi AD, Garnham A, Franco M, Marino ND, Panas MW, Ferguson DJ, Enciso M, O'Neill MT, Lopaticki S, Stewart RJ, Dewson G, Smyth GK, Smith BJ, Masters SL, Boothroyd JC, Boddey JA, Tonkin CJ (2015) An aspartyl protease defines a novel pathway for export of Toxoplasma proteins into the host cell. Elife 4:e10809","journal-title":"Elife"},{"key":"6163_CR13","doi-asserted-by":"publisher","first-page":"1358","DOI":"10.1128\/AAC.43.6.1358","volume":"43","author":"V Conseil","year":"1999","unstructured":"Conseil V, Soete M, Dubremetz JF (1999) Serine protease inhibitors block invasion of host cells by Toxoplasma gondii. Antimicrob Agents Chemother 43:1358\u20131361","journal-title":"Antimicrob Agents Chemother"},{"key":"6163_CR14","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1182\/blood-2005-02-0666","volume":"107","author":"N Courret","year":"2006","unstructured":"Courret N, Darche S, Sonigo P, Milon G, Buzoni-Gatel D, Tardieux I (2006) CD11c- and CD11b-expressing mouse leukocytes transport single Toxoplasma gondii tachyzoites to the brain. Blood 107:309\u2013316","journal-title":"Blood"},{"key":"6163_CR15","doi-asserted-by":"publisher","first-page":"372","DOI":"10.3389\/fcimb.2017.00372","volume":"7","author":"P Cuellar","year":"2017","unstructured":"Cuellar P, Hernandez-Nava E, Garcia-Rivera G, Chavez-Munguia B, Schnoor M, Betanzos A, Orozco E (2017) Entamoeba histolytica EhCP112 dislocates and degrades Claudin-1 and Claudin-2 at tight junctions of the intestinal epithelium. Front Cell Infect Microbiol 7:372","journal-title":"Front Cell Infect Microbiol"},{"key":"6163_CR16","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1007\/s10930-010-9250-8","volume":"29","author":"KP Sousa de","year":"2010","unstructured":"de Sousa KP, Atouguia J, Silva MS (2010) Partial biochemical characterization of a metalloproteinase from the bloodstream forms of Trypanosoma brucei brucei parasites. Protein J 29:283\u2013289","journal-title":"Protein J"},{"key":"6163_CR17","doi-asserted-by":"publisher","first-page":"3523","DOI":"10.1074\/jbc.M112.443697","volume":"288","author":"Z Dou","year":"2013","unstructured":"Dou Z, Coppens I, Carruthers VB (2013) Non-canonical maturation of two papain-family proteases in Toxoplasma gondii. J Biol Chem 288:3523\u20133534","journal-title":"J Biol Chem"},{"key":"6163_CR18","doi-asserted-by":"publisher","first-page":"e01188","DOI":"10.1128\/mBio.01188-14","volume":"5","author":"Z Dou","year":"2014","unstructured":"Dou Z, McGovern OL, Di Cristina M, Carruthers VB (2014) Toxoplasma gondii ingests and digests host cytosolic proteins. MBio 5:e01188\u2013e01114","journal-title":"MBio"},{"key":"6163_CR19","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1051\/parasite\/2018025","volume":"25","author":"S Escotte-Binet","year":"2018","unstructured":"Escotte-Binet S, Huguenin A, Aubert D, Martin AP, Kaltenbach M, Florent I, Villena I (2018) Metallopeptidases of Toxoplasma gondii: in silico identification and gene expression. Parasite 25:26","journal-title":"Parasite"},{"key":"6163_CR20","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1007\/s007920070033","volume":"4","author":"MI Gimenez","year":"2000","unstructured":"Gimenez MI, Studdert CA, Sanchez JJ, De Castro RE (2000) Extracellular protease of Natrialba magadii: purification and biochemical characterization. Extremophiles 4:181\u2013188","journal-title":"Extremophiles"},{"key":"6163_CR21","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1111\/j.1600-0854.2011.01308.x","volume":"13","author":"BE Hajagos","year":"2012","unstructured":"Hajagos BE, Turetzky JM, Peng ED, Cheng SJ, Ryan CM, Souda P, Whitelegge JP, Lebrun M, Dubremetz JF, Bradley PJ (2012) Molecular dissection of novel trafficking and processing of the Toxoplasma gondii rhoptry metalloprotease toxolysin-1. Traffic 13:292\u2013304","journal-title":"Traffic"},{"key":"6163_CR22","doi-asserted-by":"publisher","first-page":"505","DOI":"10.3347\/kjp.2017.55.5.505","volume":"55","author":"Y Han","year":"2017","unstructured":"Han Y, Zhou A, Lu G, Zhao G, Sha W, Wang L, Guo J, Zhou J, Zhou H, Cong H, He S (2017) DNA vaccines encoding Toxoplasma gondii Cathepsin C 1 induce protection against toxoplasmosis in mice. Korean J Parasitol 55:505\u2013512","journal-title":"Korean J Parasitol"},{"key":"6163_CR23","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1111\/pim.12163","volume":"37","author":"KS Harker","year":"2015","unstructured":"Harker KS, Ueno N, Lodoen MB (2015) Toxoplasma gondii dissemination: a parasite's journey through the infected host. Parasite Immunol 37:141\u2013149","journal-title":"Parasite Immunol"},{"key":"6163_CR24","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.exppara.2004.05.004","volume":"107","author":"R Hern\u00e1ndez-Guti\u00e9rrez","year":"2004","unstructured":"Hern\u00e1ndez-Guti\u00e9rrez R, \u00c1vila-Gonz\u00e1lez L, Ortega-L\u00f3pez J, Cruz-Talonia F, G\u00f3mez-Guti\u00e9rrez G, Arroyo R (2004) Trichomonas vaginalis: characterization of a 39-kDa cysteine proteinase found in patient vaginal secretions. Exp Parasitol 107:125\u2013135","journal-title":"Exp Parasitol"},{"key":"6163_CR25","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/0003-2697(80)90338-3","volume":"102","author":"C Heussen","year":"1980","unstructured":"Heussen C, Dowdle EB (1980) Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Anal Biochem 102:196\u2013202","journal-title":"Anal Biochem"},{"key":"6163_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.molbiopara.2009.11.005","volume":"170","author":"H Jia","year":"2010","unstructured":"Jia H, Nishikawa Y, Luo Y, Yamagishi J, Sugimoto C, Xuan X (2010) Characterization of a leucine aminopeptidase from Toxoplasma gondii. Mol Biochem Parasitol 170:1\u20136","journal-title":"Mol Biochem Parasitol"},{"key":"6163_CR27","doi-asserted-by":"publisher","first-page":"1611","DOI":"10.1016\/j.ijpara.2009.06.004","volume":"39","author":"NA Khan","year":"2009","unstructured":"Khan NA, Siddiqui R (2009) Acanthamoeba affects the integrity of human brain microvascular endothelial cells and degrades the tight junction proteins. Int J Parasitol 39:1611\u20131616","journal-title":"Int J Parasitol"},{"key":"6163_CR28","doi-asserted-by":"publisher","first-page":"1792","DOI":"10.1111\/j.1462-5822.2010.01509.x","volume":"12","author":"V Lagal","year":"2010","unstructured":"Lagal V, Binder EM, Huynh MH, Kafsack BF, Harris PK, Diez R, Chen D, Cole RN, Carruthers VB, Kim K (2010) Toxoplasma gondii protease TgSUB1 is required for cell surface processing of micronemal adhesive complexes and efficient adhesion of tachyzoites. Cell Microbiol 12:1792\u20131808","journal-title":"Cell Microbiol"},{"key":"6163_CR29","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.molbiopara.2011.01.009","volume":"177","author":"J Laliberte","year":"2011","unstructured":"Laliberte J, Carruthers VB (2011) Toxoplasma gondii toxolysin 4 is an extensively processed putative metalloproteinase secreted from micronemes. Mol Biochem Parasitol 177:49\u201356","journal-title":"Mol Biochem Parasitol"},{"key":"6163_CR30","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/j.ijpara.2015.01.003","volume":"45","author":"LP Leroux","year":"2015","unstructured":"Leroux LP, Dasanayake D, Rommereim LM, Fox BA, Bzik DJ, Jardim A, Dzierszinski FS (2015) Secreted Toxoplasma gondii molecules interfere with expression of MHC-II in interferon gamma-activated macrophages. Int J Parasitol 45:319\u2013332","journal-title":"Int J Parasitol"},{"key":"6163_CR31","doi-asserted-by":"publisher","first-page":"1696","DOI":"10.1002\/pmic.200800564","volume":"9","author":"GZ Li","year":"2009","unstructured":"Li GZ, Vissers JP, Silva JC, Golick D, Gorenstein MV, Geromanos SJ (2009) Database searching and accounting of multiplexed precursor and product ion spectra from the data independent analysis of simple and complex peptide mixtures. Proteomics 9:1696\u20131719","journal-title":"Proteomics"},{"key":"6163_CR32","doi-asserted-by":"publisher","first-page":"789","DOI":"10.7150\/thno.18133","volume":"7","author":"P Li","year":"2017","unstructured":"Li P, Kaslan M, Lee SH, Yao J, Gao Z (2017) Progress in exosome isolation techniques. Theranostics 7:789\u2013804","journal-title":"Theranostics"},{"key":"6163_CR33","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1645\/0022-3395(2002)088[1113:CONCPN]2.0.CO;2","volume":"88","author":"K Louie","year":"2002","unstructured":"Louie K, Nordhausen R, Robinson TW, Barr BC, Conrad PA (2002) Characterization of Neospora caninum protease, NcSUB1 (NC-P65), with rabbit anti-N54. J Parasitol 88:1113\u20131119","journal-title":"J Parasitol"},{"key":"6163_CR34","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1007\/978-1-4939-7111-4_9","volume":"1626","author":"MG Madanan","year":"2017","unstructured":"Madanan MG, Mechoor A (2017) Detection and characterization of bacterial proteinases using zymography. Methods Mol Biol 1626:103\u2013114","journal-title":"Methods Mol Biol"},{"key":"6163_CR35","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1128\/IAI.71.2.1008-1010.2003","volume":"71","author":"BS McGwire","year":"2003","unstructured":"McGwire BS, Chang KP, Engman DM (2003) Migration through the extracellular matrix by the parasitic protozoan Leishmania is enhanced by surface metalloprotease gp63. Infect Immun 71:1008\u20131010","journal-title":"Infect Immun"},{"key":"6163_CR36","doi-asserted-by":"publisher","first-page":"e1006351","DOI":"10.1371\/journal.ppat.1006351","volume":"13","author":"OA Mendez","year":"2017","unstructured":"Mendez OA, Koshy AA (2017) Toxoplasma gondii: entry, association, and physiological influence on the central nervous system. PLoS Pathog 13:e1006351","journal-title":"PLoS Pathog"},{"key":"6163_CR37","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1046\/j.1365-2958.2003.03604.x","volume":"49","author":"SA Miller","year":"2003","unstructured":"Miller SA, Thathy V, Ajioka JW, Blackman MJ, Kim K (2003) TgSUB2 is a Toxoplasma gondii rhoptry organelle processing proteinase. Mol Microbiol 49:883\u2013894","journal-title":"Mol Microbiol"},{"key":"6163_CR38","doi-asserted-by":"publisher","first-page":"1358","DOI":"10.1111\/j.1365-2958.2010.07165.x","volume":"76","author":"K Miranda","year":"2010","unstructured":"Miranda K, Pace DA, Cintron R, Rodrigues JC, Fang J, Smith A, Rohloff P, Coelho E, de Haas F, de Souza W, Coppens I, Sibley LD, Moreno SN (2010) Characterization of a novel organelle in Toxoplasma gondii with similar composition and function to the plant vacuole. Mol Microbiol 76:1358\u20131375","journal-title":"Mol Microbiol"},{"key":"6163_CR39","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1111\/j.1550-7408.1996.tb04491.x","volume":"43","author":"R Mondrag\u00f3n","year":"1996","unstructured":"Mondrag\u00f3n R, Frixione E (1996) Ca(2+)-dependence of conoid extrusion in Toxoplasma gondii tachyzoites. J Eukaryot Microbiol 43:120\u2013127","journal-title":"J Eukaryot Microbiol"},{"key":"6163_CR40","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1007\/978-1-4939-7111-4_20","volume":"1626","author":"JFS Monte","year":"2017","unstructured":"Monte JFS, Moreno CJG, Monteiro J, de Oliveira Rocha HA, Ribeiro AR, Silva MS (2017) Use of zymography in trypanosomiasis studies. Methods Mol Biol 1626:213\u2013220","journal-title":"Methods Mol Biol"},{"key":"6163_CR41","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1002\/elps.1150090603","volume":"9","author":"V Neuhoff","year":"1988","unstructured":"Neuhoff V, Arold N, Taube D, Ehrhardt W (1988) Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9:255\u2013262","journal-title":"Electrophoresis"},{"key":"6163_CR42","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1016\/j.exppara.2010.01.023","volume":"125","author":"AC Nogueira de Melo","year":"2010","unstructured":"Nogueira de Melo AC, de Souza EP, Elias CG, dos Santos AL, Branquinha MH, d'Avila-Levy CM, dos Reis FC, Costa TF, Lima AP, de Souza Pereira MC, Meirelles MN, Vermelho AB (2010) Detection of matrix metallopeptidase-9-like proteins in Trypanosoma cruzi. Exp Parasitol 125:256\u2013263","journal-title":"Exp Parasitol"},{"key":"6163_CR43","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1017\/S0031182015001973","volume":"143","author":"AR Nogueira","year":"2016","unstructured":"Nogueira AR, Leve F, Morgado-Diaz J, Tedesco RC, Pereira MC (2016) Effect of Toxoplasma gondii infection on the junctional complex of retinal pigment epithelial cells. Parasitology 143:568\u2013575","journal-title":"Parasitology"},{"key":"6163_CR44","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1017\/S0031182012001448","volume":"140","author":"FO Oliveira-Jr","year":"2013","unstructured":"Oliveira-Jr FO, Alves CR, Silva FS, Cortes LM, Toma L, Boucas RI, Aguilar T, Nader HB, Pereira MC (2013) Trypanosoma cruzi heparin-binding proteins present a flagellar membrane localization and serine proteinase activity. Parasitology 140:171\u2013180","journal-title":"Parasitology"},{"key":"6163_CR45","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2012\/748206","volume":"2012","author":"C Pi\u00f1a-V\u00e1zquez","year":"2012","unstructured":"Pi\u00f1a-V\u00e1zquez C, Reyes-L\u00f3pez M, Ort\u00edz-Estrada G, de la Garza M, Serrano-Luna J (2012) Host-parasite interaction: parasite-derived and -induced proteases that degrade human extracellular matrix. J Parasitol Res 2012:1\u201324","journal-title":"J Parasitol Res"},{"key":"6163_CR46","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1515\/hsz-2012-0174","volume":"393","author":"M Potempa","year":"2012","unstructured":"Potempa M, Potempa J (2012) Protease-dependent mechanisms of complement evasion by bacterial pathogens. Biol Chem 393:873\u2013888","journal-title":"Biol Chem"},{"key":"6163_CR47","doi-asserted-by":"publisher","first-page":"6436","DOI":"10.4049\/jimmunol.175.10.6436","volume":"175","author":"M Prato","year":"2005","unstructured":"Prato M, Giribaldi G, Polimeni M, Gallo V, Arese P (2005) Phagocytosis of hemozoin enhances matrix metalloproteinase-9 activity and TNF-alpha production in human monocytes: role of matrix metalloproteinases in the pathogenesis of falciparum malaria. J Immunol 175:6436\u20136442","journal-title":"J Immunol"},{"key":"6163_CR48","doi-asserted-by":"publisher","first-page":"25791","DOI":"10.1074\/jbc.M202659200","volume":"277","author":"X Que","year":"2002","unstructured":"Que X, Ngo H, Lawton J, Gray M, Liu Q, Engel J, Brinen L, Ghosh P, Joiner KA, Reed SL (2002) The cathepsin B of Toxoplasma gondii, toxopain-1, is critical for parasite invasion and rhoptry protein processing. J Biol Chem 277:25791\u201325797","journal-title":"J Biol Chem"},{"key":"6163_CR49","doi-asserted-by":"publisher","first-page":"4994","DOI":"10.1074\/jbc.M606764200","volume":"282","author":"X Que","year":"2007","unstructured":"Que X, Engel JC, Ferguson D, Wunderlich A, Tomavo S, Reed SL (2007) Cathepsin Cs are key for the intracellular survival of the protozoan parasite, Toxoplasma gondii. J Biol Chem 282:4994\u20135003","journal-title":"J Biol Chem"},{"key":"6163_CR50","doi-asserted-by":"publisher","first-page":"964","DOI":"10.1023\/A:1011953405272","volume":"15","author":"B Rothen-Rutishauser","year":"1998","unstructured":"Rothen-Rutishauser B, Kramer SD, Braun A, Gunthert M, Wunderli-Allenspach H (1998) MDCK cell cultures as an epithelial in vitro model: cytoskeleton and tight junctions as indicators for the definition of age-related stages by confocal microscopy. Pharm Res 15:964\u2013971","journal-title":"Pharm Res"},{"key":"6163_CR51","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1186\/1756-3305-6-50","volume":"6","author":"L Saboia-Vahia","year":"2013","unstructured":"Saboia-Vahia L, Borges-Veloso A, Mesquita-Rodrigues C, Cuervo P, Dias-Lopes G, Britto C, Silva AP, De Jesus JB (2013) Trypsin-like serine peptidase profiles in the egg, larval, and pupal stages of Aedes albopictus. Parasit Vectors 6:50","journal-title":"Parasit Vectors"},{"key":"6163_CR52","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1186\/1756-3305-7-253","volume":"7","author":"L Saboia-Vahia","year":"2014","unstructured":"Saboia-Vahia L, Cuervo P, Borges-Veloso A, de Souza NP, Britto C, Dias-Lopes G, De Jesus JB (2014) The midgut of Aedes albopictus females expresses active trypsin-like serine peptidases. Parasit Vectors 7:253","journal-title":"Parasit Vectors"},{"key":"6163_CR53","doi-asserted-by":"publisher","first-page":"1500","DOI":"10.3748\/wjg.v16.i12.1500","volume":"16","author":"CL Sampieri","year":"2010","unstructured":"Sampieri CL, de la Pena S, Ochoa-Lara M, Zenteno-Cuevas R, Leon-Cordoba K (2010) Expression of matrix metalloproteinases 2 and 9 in human gastric cancer and superficial gastritis. World J Gastroenterol 16:1500\u20131505","journal-title":"World J Gastroenterol"},{"key":"6163_CR54","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.vetpar.2011.11.035","volume":"186","author":"SH Schuindt","year":"2012","unstructured":"Schuindt SH, Oliveira BC, Pimentel PM, Resende TL, Retamal CA, DaMatta RA, Seipel D, Arnholdt AC (2012) Secretion of multi-protein migratory complex induced by Toxoplasma gondii infection in macrophages involves the uPA\/uPAR activation system. Vet Parasitol 186:207\u2013215","journal-title":"Vet Parasitol"},{"key":"6163_CR55","doi-asserted-by":"publisher","first-page":"2856","DOI":"10.1038\/nprot.2006.468","volume":"1","author":"A Shevchenko","year":"2006","unstructured":"Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M (2006) In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 1:2856\u20132860","journal-title":"Nat Protoc"},{"key":"6163_CR56","doi-asserted-by":"publisher","first-page":"e0006512","DOI":"10.1371\/journal.pntd.0006512","volume":"12","author":"JL Siqueira-Neto","year":"2018","unstructured":"Siqueira-Neto JL, Debnath A, McCall LI, Bernatchez JA, Ndao M, Reed SL, Rosenthal PJ (2018) Cysteine proteases in protozoan parasites. PLoS Negl Trop Dis 12:e0006512","journal-title":"PLoS Negl Trop Dis"},{"key":"6163_CR57","doi-asserted-by":"publisher","first-page":"165","DOI":"10.3347\/kjp.2003.41.3.165","volume":"41","author":"KJ Song","year":"2003","unstructured":"Song KJ, Nam HW (2003) Protease activity of 80\u00a0kDa protein secreted from the apicomplexan parasite Toxoplasma gondii. Korean J Parasitol 41:165\u2013169","journal-title":"Korean J Parasitol"},{"key":"6163_CR58","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","volume":"30","author":"AM Tenter","year":"2000","unstructured":"Tenter AM, Heckeroth AR, Weiss LM (2000) Toxoplasma gondii: from animals to humans. Int J Parasitol 30:1217\u20131258","journal-title":"Int J Parasitol"},{"key":"6163_CR59","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.ijbiomac.2016.01.060","volume":"86","author":"SE Tork","year":"2016","unstructured":"Tork SE, Shahein YE, El-Hakim AE, Abdel-Aty AM, Aly MM (2016) Purification and partial characterization of serine-metallokeratinase from a newly isolated Bacillus pumilus NRC21. Int J Biol Macromol 86:189\u2013196","journal-title":"Int J Biol Macromol"},{"key":"6163_CR60","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/978-1-4939-7111-4_3","volume":"1626","author":"J Wilkesman","year":"2017","unstructured":"Wilkesman J (2017) Cysteine protease zymography: brief review. Methods Mol Biol 1626:25\u201331","journal-title":"Methods Mol Biol"},{"key":"6163_CR61","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1186\/1471-2334-13-207","volume":"13","author":"G Zhao","year":"2013","unstructured":"Zhao G, Zhou A, Lv G, Meng M, Sun M, Bai Y, Han Y, Wang L, Zhou H, Cong H, Zhao Q, Zhu XQ, He S (2013) Toxoplasma gondii cathepsin proteases are undeveloped prominent vaccine antigens against toxoplasmosis. BMC Infect Dis 13:207","journal-title":"BMC Infect Dis"},{"key":"6163_CR62","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.actatropica.2017.02.030","volume":"171","author":"G Zhao","year":"2017","unstructured":"Zhao G, Song X, Kong X, Zhang N, Qu S, Zhu W, Yang Y, Wang Q (2017) Immunization with Toxoplasma gondii aspartic protease 3 increases survival time of infected mice. Acta Trop 171:17\u201323","journal-title":"Acta Trop"},{"key":"6163_CR63","doi-asserted-by":"publisher","first-page":"34448","DOI":"10.1038\/srep34448","volume":"6","author":"J Zheng","year":"2016","unstructured":"Zheng J, Cheng Z, Jia H, Zheng Y (2016) Characterization of aspartyl aminopeptidase from Toxoplasma gondii. Sci Rep 6:34448","journal-title":"Sci Rep"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00436-018-6163-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-018-6163-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-018-6163-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T08:01:39Z","timestamp":1575273699000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00436-018-6163-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,1]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["6163"],"URL":"https:\/\/doi.org\/10.1007\/s00436-018-6163-5","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,1]]},"assertion":[{"value":"4 June 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 November 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflicts of interest with the contents of this article.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}