{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T20:26:39Z","timestamp":1771273599792,"version":"3.50.1"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2019,7,9]],"date-time":"2019-07-09T00:00:00Z","timestamp":1562630400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2019,7,9]],"date-time":"2019-07-09T00:00:00Z","timestamp":1562630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2019,9,1]]},"DOI":"10.1007\/s00436-019-06388-6","type":"journal-article","created":{"date-parts":[[2019,7,9]],"date-time":"2019-07-09T02:02:44Z","timestamp":1562637764000},"page":"2715-2721","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Serological and molecular investigation of selected parasitic pathogens in European brown hare (Lepus europaeus) in Greece: inferring the ecological niche of Toxoplasma gondii and Leishmania infantum in hares"],"prefix":"10.1007","volume":"118","author":[{"given":"Constantina N.","family":"Tsokana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christos","family":"Sokos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexios","family":"Giannakopoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Periklis","family":"Birtsas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Labrini V.","family":"Athanasiou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Valiakos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Sofia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios C.","family":"Chatzopoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Kantere","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassiliki","family":"Spyrou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angeliki Rodi","family":"Burriel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charalambos","family":"Billinis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,7,9]]},"reference":[{"key":"6388_CR1","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1017\/S0950268809991270","volume":"138","author":"E Afonso","year":"2010","unstructured":"Afonso E, Thulliez P, Gilot-Fromont E (2010) Local meteorological conditions, dynamics of seroconversion to Toxoplasma gondii in cats (Felis catus) and oocyst burden in a rural environment. Epidemiol Infect 138:1105\u20131113. https:\/\/doi.org\/10.1017\/S0950268809991270","journal-title":"Epidemiol Infect"},{"key":"6388_CR2","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/j.actatropica.2012.02.003","volume":"122","author":"LV Athanasiou","year":"2012","unstructured":"Athanasiou LV, Kontos VI, Saridomichelakis MN et al (2012) A cross-sectional sero-epidemiological study of canine leishmaniasis in Greek mainland. Acta Trop 122:291\u2013295. https:\/\/doi.org\/10.1016\/j.actatropica.2012.02.003","journal-title":"Acta Trop"},{"key":"6388_CR3","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.vetpar.2010.03.033","volume":"171","author":"D Aubert","year":"2010","unstructured":"Aubert D, Ajzenberg D, Richomme C et al (2010) Molecular and biological characteristics of Toxoplasma gondii isolates from wildlife in France. Vet Parasitol 171:346\u2013349. https:\/\/doi.org\/10.1016\/j.vetpar.2010.03.033","journal-title":"Vet Parasitol"},{"key":"6388_CR4","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.vetpar.2010.03.002","volume":"171","author":"E B\u00e1rtov\u00e1","year":"2010","unstructured":"B\u00e1rtov\u00e1 E, Sedl\u00e1k K, Treml F et al (2010) Neospora caninum and Toxoplasma gondii antibodies in European brown hares in the Czech Republic, Slovakia and Austria. Vet Parasitol 171:155\u2013158. https:\/\/doi.org\/10.1016\/j.vetpar.2010.03.002","journal-title":"Vet Parasitol"},{"key":"6388_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1469-7998.2011.00887.x","volume":"287","author":"PW Bateman","year":"2012","unstructured":"Bateman PW, Fleming PA (2012) Big city life: carnivores in urban environments: urban carnivores. J Zool 287:1\u201323. https:\/\/doi.org\/10.1111\/j.1469-7998.2011.00887.x","journal-title":"J Zool"},{"key":"6388_CR6","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1186\/1756-3305-3-31","volume":"3","author":"L Chamaill\u00e9","year":"2010","unstructured":"Chamaill\u00e9 L, Tran A, Meunier A et al (2010) Environmental risk mapping of canine leishmaniasis in France. Parasit Vectors 3:31. https:\/\/doi.org\/10.1186\/1756-3305-3-31","journal-title":"Parasit Vectors"},{"key":"6388_CR7","doi-asserted-by":"publisher","first-page":"728","DOI":"10.1016\/j.jfms.2008.01.009","volume":"11","author":"A Diakou","year":"2009","unstructured":"Diakou A, Papadopoulos E, Lazarides K (2009) Specific anti-Leishmania spp. antibodies in stray cats in Greece. J Feline Med Surg 11:728\u2013730. https:\/\/doi.org\/10.1016\/j.jfms.2008.01.009","journal-title":"J Feline Med Surg"},{"key":"6388_CR8","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/j.apjtm.2016.03.032","volume":"9","author":"VV Ebani","year":"2016","unstructured":"Ebani VV, Poli A, Rocchigiani G et al (2016) Serological survey on some pathogens in wild brown hares (Lepus europaeus) in Central Italy. Asian Pac J Trop Med 9:465\u2013469. https:\/\/doi.org\/10.1016\/j.apjtm.2016.03.032","journal-title":"Asian Pac J Trop Med"},{"key":"6388_CR9","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1006\/expr.2001.4592","volume":"97","author":"NO El Tai","year":"2001","unstructured":"El Tai NO, El Fari M, Mauricio I et al (2001) Leishmania donovani: intraspecific polymorphisms of Sudanese isolates revealed by PCR-based analyses and DNA sequencing. Exp Parasitol 97:35\u201344. https:\/\/doi.org\/10.1006\/expr.2001.4592","journal-title":"Exp Parasitol"},{"key":"6388_CR10","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.vetpar.2013.01.061","volume":"196","author":"X Fern\u00e1ndez-Aguilar","year":"2013","unstructured":"Fern\u00e1ndez-Aguilar X, Alzaga V, Villan\u00faa D et al (2013) Epidemiology and prevalence of Toxoplasma gondii infection in the Iberian hare (Lepus granatensis). Vet Parasitol 196:194\u2013198. https:\/\/doi.org\/10.1016\/j.vetpar.2013.01.061","journal-title":"Vet Parasitol"},{"key":"6388_CR11","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/S0304-4017(03)00201-2","volume":"115","author":"E Ferroglio","year":"2003","unstructured":"Ferroglio E, Trisciuoglio A (2003) Antibodies to Neospora caninum in European brown hare (Lepus europaeus). Vet Parasitol 115:75\u201378. https:\/\/doi.org\/10.1016\/S0304-4017(03)00201-2","journal-title":"Vet Parasitol"},{"key":"6388_CR12","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1017\/S0031182016000378","volume":"143","author":"A Giannakopoulos","year":"2016","unstructured":"Giannakopoulos A, Tsokana CN, Pervanidou D et al (2016) Environmental parameters as risk factors for human and canine Leishmania infection in Thessaly, Central Greece. Parasitology 143:1179\u20131186. https:\/\/doi.org\/10.1017\/S0031182016000378","journal-title":"Parasitology"},{"key":"6388_CR13","doi-asserted-by":"publisher","first-page":"27","DOI":"10.12681\/jhvms.15553","volume":"68","author":"A Giannakopoulos","year":"2017","unstructured":"Giannakopoulos A, Tsokana CN, Papadopoulos E et al (2017) Molecular investigation and geographical distribution of Leishmania spp. infection in stray and owned cats (Felis catus) in Thessaly, central Greece. J Hellenic Vet Med Soc 68:27\u201334","journal-title":"J Hellenic Vet Med Soc"},{"key":"6388_CR14","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/S0020-7519(99)00170-8","volume":"30","author":"W Homan","year":"2000","unstructured":"Homan W, Vercammen M, De Braekeleer J, Verschueren H (2000) Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative. Int J Parasitol 30:69\u201375. https:\/\/doi.org\/10.1016\/S0020-7519(99)00170-8","journal-title":"Int J Parasitol"},{"key":"6388_CR15","doi-asserted-by":"publisher","first-page":"1574","DOI":"10.1017\/S0031182015001158","volume":"142","author":"S Karayiannis","year":"2015","unstructured":"Karayiannis S, Ntais P, Messaritakis I et al (2015) Detection of Leishmania infantum in red foxes (Vulpes vulpes) in Central Greece. Parasitology 142:1574\u20131578. https:\/\/doi.org\/10.1017\/S0031182015001158","journal-title":"Parasitology"},{"key":"6388_CR16","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1007\/s00436-014-3929-2","volume":"113","author":"J Mill\u00e1n","year":"2014","unstructured":"Mill\u00e1n J, Ferroglio E, Solano-Gallego L (2014) Role of wildlife in the epidemiology of Leishmania infantum infection in Europe. Parasitol Res 113:2005\u20132014. https:\/\/doi.org\/10.1007\/s00436-014-3929-2","journal-title":"Parasitol Res"},{"key":"6388_CR17","doi-asserted-by":"crossref","first-page":"2850","DOI":"10.1128\/jcm.34.11.2850-2852.1996","volume":"34","author":"N M\u00fcller","year":"1996","unstructured":"M\u00fcller N, Zimmermann V, Hentrich B, Gottstein B (1996) Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. J Clin Microbiol 34:2850\u20132852","journal-title":"J Clin Microbiol"},{"key":"6388_CR18","doi-asserted-by":"publisher","first-page":"906","DOI":"10.4269\/ajtmh.13-0070","volume":"89","author":"P Ntais","year":"2013","unstructured":"Ntais P, Sifaki-Pistola D, Christodoulou V et al (2013) Leishmaniases in Greece. Am J Trop Med Hyg 89:906\u2013915. https:\/\/doi.org\/10.4269\/ajtmh.13-0070","journal-title":"Am J Trop Med Hyg"},{"key":"6388_CR19","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.ecolmodel.2005.03.026","volume":"190","author":"SJ Phillips","year":"2006","unstructured":"Phillips SJ, Anderson RP, Schapire RE (2006) Maximum entropy modeling of species geographic distributions. Ecol Model 190:231\u2013259. https:\/\/doi.org\/10.1016\/j.ecolmodel.2005.03.026","journal-title":"Ecol Model"},{"key":"6388_CR20","first-page":"341","volume-title":"Diseases of wild-living hares (Lepus europaeus P.) in Tuscany","author":"A Poli","year":"1987","unstructured":"Poli A, Mancianti F, Marconcini A et al (1987) In: Ippen R, Schroder HD (eds) Diseases of wild-living hares (Lepus europaeus P.) in Tuscany. Akademie- Verlag, Berlin, pp 341\u2013346"},{"key":"6388_CR21","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1111\/j.1365-2915.2012.01033.x","volume":"27","author":"M Quintana","year":"2013","unstructured":"Quintana M, Salom\u00f3N O, Guerra R et al (2013) Phlebotominae of epidemiological importance in cutaneous leishmaniasis in northwestern Argentina: risk maps and ecological niche models. Med Vet Entomol 27:39\u201348. https:\/\/doi.org\/10.1111\/j.1365-2915.2012.01033.x","journal-title":"Med Vet Entomol"},{"key":"6388_CR22","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.meegid.2018.02.005","volume":"59","author":"G Rocchigiani","year":"2018","unstructured":"Rocchigiani G, Ebani VV, Nardoni S et al (2018) Molecular survey on the occurrence of arthropod-borne pathogens in wild brown hares (Lepus europaeus) from Central Italy. Infect Genet Evol 59:142\u2013147. https:\/\/doi.org\/10.1016\/j.meegid.2018.02.005","journal-title":"Infect Genet Evol"},{"key":"6388_CR23","first-page":"20541","volume":"18","author":"F Ruiz-Fons","year":"2013","unstructured":"Ruiz-Fons F, Ferroglio E, Gort\u00e1zar C (2013) Leishmania infantum in free-ranging hares, Spain, 2004\u20132010. Euro Surveill Bull Eur Sur Mal Transm Eur Commun Dis Bull 18:20541","journal-title":"Euro Surveill Bull Eur Sur Mal Transm Eur Commun Dis Bull"},{"key":"6388_CR24","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1177\/1098612X15589358","volume":"18","author":"CSA Soares","year":"2016","unstructured":"Soares CSA, Duarte SC, Sousa SR (2016) What do we know about feline leishmaniosis? J Feline Med Surg 18:435\u2013442. https:\/\/doi.org\/10.1177\/1098612X15589358","journal-title":"J Feline Med Surg"},{"key":"6388_CR25","doi-asserted-by":"publisher","first-page":"e000077","DOI":"10.1136\/vetreco-2014-000077","volume":"2","author":"A Touloudi","year":"2015","unstructured":"Touloudi A, Valiakos G, Athanasiou LV et al (2015) A serosurvey for selected pathogens in Greek European wild boar. Vet Rec Open 2:e000077. https:\/\/doi.org\/10.1136\/vetreco-2014-000077","journal-title":"Vet Rec Open"},{"key":"6388_CR26","doi-asserted-by":"publisher","first-page":"451","DOI":"10.17221\/2050-VETMED","volume":"52","author":"F Treml","year":"2007","unstructured":"Treml F, Pikula J, Bandouchova H, Horakova J (2007) European brown hare as a potential source of zoonotic agents. Vet Med (Praha) 52:451\u2013456","journal-title":"Vet Med (Praha)"},{"key":"6388_CR27","doi-asserted-by":"publisher","first-page":"1523","DOI":"10.1111\/tmi.12982","volume":"22","author":"\u0399 Tsakmakidis","year":"2017","unstructured":"Tsakmakidis \u0399, Angelopoulou K, Dovas CI et al (2017) Leishmania infection in rodents in Greece. Trop Med Int Health TM IH 22:1523\u20131532. https:\/\/doi.org\/10.1111\/tmi.12982","journal-title":"Trop Med Int Health TM IH"},{"key":"6388_CR28","doi-asserted-by":"publisher","unstructured":"Tsokana CN, Sokos C, Giannakopoulos A et al (2015) First evidence of Leishmania infection in European brown hare (Lepus europaeus) in Greece: GIS analysis and phylogenetic position within the Leishmania spp. Parasitol Res. https:\/\/doi.org\/10.1007\/s00436-015-4749-8","DOI":"10.1007\/s00436-015-4749-8"},{"key":"6388_CR29","doi-asserted-by":"publisher","unstructured":"Yan C, Liang L-J, Zheng K-Y, Zhu X-Q (2016) Impact of environmental factors on the emergence, transmission and distribution of Toxoplasma gondii. Parasit Vectors 9. https:\/\/doi.org\/10.1186\/s13071-016-1432-6","DOI":"10.1186\/s13071-016-1432-6"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-019-06388-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00436-019-06388-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-019-06388-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T06:04:15Z","timestamp":1744178655000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00436-019-06388-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,9]]},"references-count":29,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9,1]]}},"alternative-id":["6388"],"URL":"https:\/\/doi.org\/10.1007\/s00436-019-06388-6","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,9]]},"assertion":[{"value":"4 October 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 June 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 July 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"In this research, the authors did not perform any studies involving human participants or animals.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The hare serum and liver samples used in the study were collected opportunistically (no active capture, killing, and sampling of wild animals specifically for this study were performed) from animals hunter-harvested by members of the Greek Hunting Federation of Macedonia and Thrace and the Greek Hunting Federation of Thessaly and Sporades during the hunting seasons according to the prerequisites of the Greek Legislation and submitted to our laboratory for Passive Wildlife Disease Surveillance. Thus, special approval was not necessary, and steps to ameliorate suffering were not applicable to this study. Research on animals as defined in the EU Ethics for Researchers document (European Commission, 2007, Ethics for Researchers\u2014Facilitating Research Excellence in FP7, Luxembourg: Office for Official Publications of the European Communities, ISBN 978-92-79-05474-7) is not applicable to this study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}