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In each study, one group served as non-treated control, whereas the other groups were treated with the Seresto\u00ae collar. All dogs were exposed to naturally<jats:italic>Bb<\/jats:italic>sl- and<jats:italic>Ap<\/jats:italic>-infected hard ticks (<jats:italic>Ixodes ricinus<\/jats:italic>,<jats:italic>Ixodes scapularis<\/jats:italic>). In study 1, tick infestation was performed on study day (SD) 63 (2\u00a0months post-treatment [p.t.]); in study 2, it was performed on SD 32 (one month p.t.) respectively SD 219 (seven months p.t.). In situ tick counts were performed 2\u00a0days after infestation. Tick counts and removals followed 6 (study 1) or 5\u00a0days (study 2) later. Blood sampling was performed for the detection of specific<jats:italic>Bb<\/jats:italic>sl and<jats:italic>Ap<\/jats:italic>antibodies and, in study 1, for the documentation of<jats:italic>Ap<\/jats:italic>DNA by PCR. Skin biopsies were examined for<jats:italic>Bb<\/jats:italic>sl by PCR and culture (only study 1). The efficacy against<jats:italic>Ixodes<\/jats:italic>spp. was 100% at all time points. In study 1, two of six non-treated dogs became infected with<jats:italic>Bb<\/jats:italic>sl, and four of six tested positive for<jats:italic>Ap<\/jats:italic>; none of the treated dogs tested positive for<jats:italic>Bb<\/jats:italic>sl or<jats:italic>Ap<\/jats:italic>. In study 2, ten of ten non-treated dogs became infected with<jats:italic>Bb<\/jats:italic>sl and<jats:italic>Ap<\/jats:italic>; none of the treated dogs tested positive for<jats:italic>Bb<\/jats:italic>sl or<jats:italic>Ap<\/jats:italic>; 100% acaricidal efficacy was shown in both studies. Transmission of<jats:italic>Bb<\/jats:italic>sl and<jats:italic>Ap<\/jats:italic>was successfully blocked for up to 7\u00a0months.<\/jats:p>","DOI":"10.1007\/s00436-019-06394-8","type":"journal-article","created":{"date-parts":[[2019,11,16]],"date-time":"2019-11-16T09:01:47Z","timestamp":1573894907000},"page":"299-315","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Prevention of transmission of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum by Ixodes spp. ticks to dogs treated with the Seresto\u00ae collar (imidacloprid 10% + flumethrin 4.5%)"],"prefix":"10.1007","volume":"119","author":[{"given":"Friederike","family":"Kr\u00e4mer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricarda","family":"H\u00fcsken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva Maria","family":"Kr\u00fcdewagen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Deuster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byron","family":"Blagburn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard K.","family":"Straubinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamie","family":"Butler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volker","family":"Fingerle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam","family":"Charles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry","family":"Settje","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Schunack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3960-3656","authenticated-orcid":false,"given":"Dorothee","family":"Stanneck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,11,16]]},"reference":[{"key":"6394_CR1","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1093\/infdis\/167.3.651","volume":"167","author":"MJG Appel","year":"1993","unstructured":"Appel MJG, Allan S, Jacobson RH, Lauderdale TL, Chang Y-F, Shin SJ, Thomford JW, Todhunter RJ, Summers BA (1993) Experimental Lyme disease in dogs produces arthritis and persistent infection. 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All institutional and national guidelines for the care and use of laboratory and study animals were followed.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics statement"}},{"value":"VF received honoraria for advanced trainings for medical professionals from DiaSorin, Mikrogen, Seramun and Virotech. The other authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}