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Due to its anaerobic metabolism, it has to quickly remove intracellular oxygen in order to avoid deactivation of essential metabolic enzymes such as oxygen-sensitive pyruvate:ferredoxin oxidoreductase (PFOR). Two major enzyme activities which are responsible for the removal, i.e. reduction, of molecular oxygen have been identified in <jats:italic>T<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic> flavin reductase, formerly designated NADPH oxidase, which indirectly reduces oxygen to hydrogen peroxide via flavin mononucleotide (FMN), and NADH oxidase which reduces oxygen to water. Flavin reductase has been identified and characterized at the gene level as well as enzymatically, but NADH oxidase has so far only been characterized enzymatically with enzyme isolated from <jats:italic>T<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic> cell extracts. In this study, we identified NADH oxidase by mass spectrometry after isolation of the enzyme from gel bands positively staining for NADH oxidase activity. In strain C1 (ATCC 30001) which is known to lack NADH oxidase activity completely, the NADH oxidase gene has a deletion at position 1540 of the open reading frame leading to a frame shift and, as a consequence, to premature termination of the encoded polypeptide.<\/jats:p>","DOI":"10.1007\/s00436-019-06572-8","type":"journal-article","created":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T09:04:31Z","timestamp":1576659871000},"page":"683-686","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of the NADH-oxidase gene in Trichomonas vaginalis"],"prefix":"10.1007","volume":"119","author":[{"given":"Aline","family":"Lamien-Meda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9128-4501","authenticated-orcid":false,"given":"David","family":"Leitsch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,12,18]]},"reference":[{"key":"6572_CR1","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/1471-2148-6-27","volume":"6","author":"JO Andersson","year":"2006","unstructured":"Andersson JO, Hirt RP, Foster PG, Roger AJ (2006) Evolution of four gene families with patchy phylogenetic distributions: influx of genes into protist genomes. 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