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Similar to the closely related parasite <jats:italic>Toxoplasma gondii<\/jats:italic>, <jats:italic>B. besnoiti<\/jats:italic> has fast proliferating properties leading to rapid host cell lysis within 24\u201330\u00a0h p.i. in vitro. Some apicomplexan parasites were demonstrated to modulate the host cellular cell cycle to successfully perform their intracellular development. As such, we recently demonstrated that <jats:italic>T. gondii<\/jats:italic> tachyzoites induce G2\/M arrest accompanied by chromosome missegregation, cell spindle alteration, formation of supernumerary centrosomes, and cytokinesis impairment when infecting primary bovine umbilical vein endothelial cells (BUVEC). Here, we follow a comparative approach by using the same host endothelial cell system for <jats:italic>B. besnoiti<\/jats:italic> infections. The current data showed that\u2014in terms of host cell cycle modulation\u2014infections of BUVEC by <jats:italic>B. besnoiti<\/jats:italic> tachyzoites indeed differ significantly from those by <jats:italic>T. gondii<\/jats:italic>. As such, cyclin expression patterns demonstrated a significant upregulation of cyclin E1 in <jats:italic>B. besnoiti\u2013<\/jats:italic>infected BUVEC, thereby indicating parasite-driven host cell stasis at G1-to-S phase transition. In line, the mitotic phase of host cell cycle was not influenced since alterations of chromosome segregation, mitotic spindle formation, and cytokinesis were not observed. In contrast to respective <jats:italic>T. gondii<\/jats:italic>\u2013related data, we furthermore found a significant upregulation of histone H3 (S10) phosphorylation in <jats:italic>B. besnoiti<\/jats:italic>\u2013infected BUVEC, thereby indicating enhanced chromosome condensation to occur in these cells. In line to altered G1\/S-transition, we here additionally showed that subcellular abundance of proliferating cell nuclear antigen (PCNA), a marker for G1 and S phase sub-stages, was affected by <jats:italic>B. besnoiti<\/jats:italic> since infected cells showed increased nuclear PCNA levels when compared with that of control cells.<\/jats:p>","DOI":"10.1007\/s00436-020-06744-x","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T03:41:43Z","timestamp":1592365303000},"page":"2563-2577","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Besnoitia besnoiti\u2013driven endothelial host cell cycle alteration"],"prefix":"10.1007","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1360-2209","authenticated-orcid":false,"given":"Zahady D.","family":"Vel\u00e1squez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Lopez-Osorio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Learta","family":"Pervizaj-Oruqaj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Herold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Hermosilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Taubert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,6,17]]},"reference":[{"key":"6744_CR1","doi-asserted-by":"crossref","unstructured":"Baker VS, Imade GE, Molta NB, Tawde P, Pam SD, Obadofin MO, Sagay SA, Egah DZ, Iya D, Afolabi BB, Baker M, Ford K, Ford R, Roux KH, Keller TCS, III (2008) Cytokine-associated neutrophil extracellular traps and antinuclear antibodies in Plasmodium falciparum infected children under six years of age Malar J 7:41","DOI":"10.1186\/1475-2875-7-41"},{"key":"6744_CR2","unstructured":"Basson PA, McCully RM, Bigalke RD (1970) Observations on the pathogenesis of bovine and antelope strains of Besnoitia besnoiti (Marotel, 1912) infection in cattle and rabbits"},{"key":"6744_CR3","first-page":"429","volume-title":"Diseases of cattle in the tropics: economic and zoonotic relevance","author":"RD Bigalke","year":"1981","unstructured":"Bigalke RD (1981) Besnoitiosis and Globidiosis. 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