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Initially, all samples were examined by phase-contrast microscopy. <jats:italic>Cryptosporidium<\/jats:italic>-positive samples (55.4%; <jats:italic>n<\/jats:italic>\u2009=\u200998) were screened by <jats:italic>gp60<\/jats:italic> PCR, resulting in 68.4% (<jats:italic>n<\/jats:italic>\u2009=\u200967) <jats:italic>C. parvum<\/jats:italic>\u2013positive samples. The remaining 31 <jats:italic>gp60<\/jats:italic>-PCR-negative and the phase-contrast microscopy negative samples (<jats:italic>n<\/jats:italic>\u2009=\u200979) were screened by PCR targeting a 700\u00a0bp fragment of the <jats:italic>18S<\/jats:italic> rRNA gene. Sequencing of the PCR products revealed the presence of <jats:italic>C. parvum<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u200969), <jats:italic>C. ryanae<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u200911), and <jats:italic>C. bovis<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20097). The latter two species have never been described in Austria. <jats:italic>C. parvum\u2013<\/jats:italic>positive samples were genotyped at the <jats:italic>gp60<\/jats:italic> gene locus, featuring four subtypes (IIaA15G2R1, IIaA21G2R1, IIaA19G2R1, IIaA14G1R1). The most frequently detected subtype IIaA15G2R1 (<jats:italic>n<\/jats:italic>\u2009=\u200952) was present in calves from 30 different farms. IIaA14G1R1 (<jats:italic>n<\/jats:italic>\u2009=\u20095) occurred on a single farm, subtype IIaA21G2R1 (<jats:italic>n<\/jats:italic>\u2009=\u20094) on two farms, and subtype IIaA19G2R1 (n\u2009=\u20094) on three farms. The results confirm the widespread occurrence of zoonotic <jats:italic>C. parvum<\/jats:italic> in diarrheic calves.<\/jats:p>","DOI":"10.1007\/s00436-020-06928-5","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T00:02:34Z","timestamp":1602720154000},"page":"4291-4295","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Cryptosporidium parvum, Cryptosporidium ryanae, and Cryptosporidium bovis in samples from calves in Austria"],"prefix":"10.1007","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5916-0610","authenticated-orcid":false,"given":"Katharina","family":"Lichtmannsperger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Harl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Freudenthaler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Hinney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Wittek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Joachim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,15]]},"reference":[{"key":"6928_CR1","doi-asserted-by":"publisher","first-page":"100010","DOI":"10.1016\/j.vpoa.2019.100010","volume":"X","author":"M \u00c5berg","year":"2019","unstructured":"\u00c5berg M, Emanuelson U, Troell K, Bj\u00f6rkman C (2019) Infection dynamics of Cryptosporidium bovis and Cryptosporidium ryanae in a Swedish dairy herd. 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