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Infection with <jats:italic>Cryptosporidium<\/jats:italic> spp. is especially common in young domestic ruminants (calves, lambs and goat kids) and has been associated with economic losses worldwide. In contrast to cattle, to date, detailed studies on <jats:italic>Cryptosporidium<\/jats:italic> infections in sheep from Europe are still limited; thus, their importance as reservoirs of <jats:italic>Cryptosporidium<\/jats:italic> species with implications on animal and public health still needs to be clarified. This study evaluates the prevalence and zoonotic potential of <jats:italic>Cryptosporidium<\/jats:italic> spp. in sheep farms in Italy. A total of 915 individual faecal samples divided into three different animal categories were collected from 61 sheep farms. Each sample was examined by microscopy of faecal smears stained by modified Ziehl-Neelsen and by biomolecular techniques. <jats:italic>Cryptosporidium<\/jats:italic> oocysts were detected in 10.1% of the animals examined and in 34.4% of the farms. The prevalence of <jats:italic>Cryptosporidium<\/jats:italic> spp. was significantly higher (\u03c7<jats:sup>2<\/jats:sup>\u00a0=\u200951.854; <jats:italic>P<\/jats:italic>\u00a0&lt;\u20090.001) in diarrhoeic samples than in pasty or normal faeces. Genotype analyses showed the presence of two <jats:italic>Cryptosporidium<\/jats:italic> species: <jats:italic>C. parvum<\/jats:italic> and <jats:italic>C. ubiquitum<\/jats:italic>. Subtyping analysis of <jats:italic>C. parvum<\/jats:italic> isolates revealed the presence of subtypes IIa15G2R1 and IIdA20G1 and of subtype XIIa for <jats:italic>C. ubiquitum<\/jats:italic>. These findings have public health implications since both <jats:italic>Cryptosporidium<\/jats:italic> species identified are considered zoonotic, and <jats:italic>C. parvum<\/jats:italic> is the second-most common <jats:italic>Cryptosporidium<\/jats:italic> species infecting humans. Our data reveal that lambs, especially those excreting diarrhoeic faeces, may be important reservoirs of <jats:italic>Cryptosporidium.<\/jats:italic> We also highlight the need to establish adequate control and monitoring programmes for the control of this infection in sheep farms primarily through coprological monitoring.<\/jats:p>","DOI":"10.1007\/s00436-020-06947-2","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T03:02:31Z","timestamp":1604372551000},"page":"4211-4218","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Cryptosporidium infections in sheep farms from Italy"],"prefix":"10.1007","volume":"119","author":[{"given":"G.","family":"Dess\u00ec","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Tamponi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6306-9978","authenticated-orcid":false,"given":"A.","family":"Varcasia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Sanna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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