{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T03:04:58Z","timestamp":1780542298390,"version":"3.54.1"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"11","license":[{"start":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T00:00:00Z","timestamp":1631145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T00:00:00Z","timestamp":1631145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001809","name":"national natural science foundation of china","doi-asserted-by":"publisher","award":["32002306"],"award-info":[{"award-number":["32002306"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the fund for shanxi \u201c1331 project\u201d"},{"DOI":"10.13039\/501100012421","name":"agricultural science and technology innovation program","doi-asserted-by":"publisher","award":["CAAS-ASTIP-2016-LVRI-03"],"award-info":[{"award-number":["CAAS-ASTIP-2016-LVRI-03"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the yunnan expert workstation","award":["202005AF150041"],"award-info":[{"award-number":["202005AF150041"]}]},{"name":"the veterinary public health innovation team of yunnan provinc","award":["202105AE160014"],"award-info":[{"award-number":["202105AE160014"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2021,11]]},"DOI":"10.1007\/s00436-021-07308-3","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T04:02:31Z","timestamp":1631160151000},"page":"3749-3759","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of functions in parasite growth and virulence of four Toxoplasma gondii genes involved in lipid synthesis by CRISPR-Cas9 system"],"prefix":"10.1007","volume":"120","author":[{"given":"Jie","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Ting","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin-Li","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany M.","family":"Elsheikha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan-Yu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Pei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2530-4628","authenticated-orcid":false,"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,9]]},"reference":[{"key":"7308_CR1","doi-asserted-by":"publisher","first-page":"1005765","DOI":"10.1371\/journal.ppat.1005765","volume":"12","author":"S Amiar","year":"2016","unstructured":"Amiar S, MacRae JI, Callahan DL, Dubois D, van Dooren GG, Shears MJ, Cesbron-Delauw MF, Mar\u00e9chal E, McConville MJ, McFadden GI, Yamaryo-Bott\u00e9 Y, Bott\u00e9 CY (2016) Apicoplast-localized lysophosphatidic acid precursor assembly is required for bulk phospholipid synthesis in Toxoplasma gondii and relies on an algal\/plant-like glycerol 3-phosphate acyltransferase. PLoS Pathog 12:1005765","journal-title":"PLoS Pathog"},{"key":"7308_CR2","doi-asserted-by":"publisher","first-page":"300","DOI":"10.3389\/fcimb.2018.00300","volume":"8","author":"MJ Bai","year":"2018","unstructured":"Bai MJ, Wang JL, Elsheikha HM, Liang QL, Chen K, Nie LB, Zhu XQ (2018) Functional characterization of dense granule proteins in Toxoplasma gondii RH strain using CRISPR-Cas9 system. Front Cell Infect Microbiol 8:300","journal-title":"Front Cell Infect Microbiol"},{"key":"7308_CR3","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1042\/BJ20050609","volume":"394","author":"C Bisanz","year":"2006","unstructured":"Bisanz C, Bastien O, Grando D, Jouhet J, Mar\u00e9chal E, Cesbron-Delauw MF (2006) Toxoplasma gondii acyl-lipid metabolism: de novo synthesis from apicoplast-generated fatty acids versus scavenging of host cell precursors. Biochem J 394:197\u2013205","journal-title":"Biochem J"},{"issue":"18","key":"7308_CR4","doi-asserted-by":"publisher","first-page":"7506","DOI":"10.1073\/pnas.1301251110","volume":"110","author":"CY Bott\u00e9","year":"2013","unstructured":"Bott\u00e9 CY, Yamaryo-Bott\u00e9 Y, Rupasinghe TW, Mullin KA, MacRae JI, Spurck TP, Kalanon M, Shears MJ, Coppel RL, Crellin PK, Mar\u00e9chal E, McConville MJ, McFadden GI (2013) Atypical lipid composition in the purified relict plastid (apicoplast) of malaria parasites. Proc Natl Acad Sci U S A 110(18):7506\u20137511","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"7308_CR5","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1111\/j.1574-6968.2007.00767.x","volume":"272","author":"X Cai","year":"2007","unstructured":"Cai X, Lorraine Fuller A, McDougald LR, Tan X, Cai J, Wang F, Sacchettini JC, Zhu G (2007) Biochemical characterization of enoyl reductase involved in Type II fatty acid synthesis in the intestinal coccidium Eimeria tenella (Phylum Apicomplexa). FEMS Microbiol Lett 272(2):238\u2013244","journal-title":"FEMS Microbiol Lett"},{"key":"7308_CR6","doi-asserted-by":"publisher","first-page":"418","DOI":"10.3389\/fcimb.2019.00418","volume":"9","author":"XZ Cao","year":"2019","unstructured":"Cao XZ, Wang JL, Elsheikha HM, Li TT, Sun LX, Liang QL, Zhang ZW, Lin RQ (2019) Characterization of the role of amylo-alpha-1,6-glucosidase protein in the infectivity of Toxoplasma gondii. Front Cell Infect Microbiol 9:418","journal-title":"Front Cell Infect Microbiol"},{"issue":"12","key":"7308_CR7","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1038\/nrmicro3139","volume":"11","author":"I Coppens","year":"2013","unstructured":"Coppens I (2013) Targeting lipid biosynthesis and salvage in apicomplexan parasites for improved chemotherapies. Nat Rev Microbiol 11(12):823\u2013835","journal-title":"Nat Rev Microbiol"},{"key":"7308_CR8","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1038\/s41426-018-0164-4","volume":"7","author":"K El Bissati","year":"2018","unstructured":"El Bissati K, Levigne P, Lykins J, Adlaoui EB, Barkat A, Berraho A, Laboudi M, El Mansouri B, Ibrahimi A, Rhajaoui M, Quinn F, Murugesan M, Seghrouchni F, G\u00f3mez-Mar\u00edn JE, Peyron F, McLeod R (2018) Global initiative for congenital toxoplasmosis: an observational and international comparative clinical analysis. Emerg Microbes Infect 7:165","journal-title":"Emerg Microbes Infect"},{"issue":"4","key":"7308_CR9","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/j.puhe.2007.08.009","volume":"122","author":"HM Elsheikha","year":"2018","unstructured":"Elsheikha HM (2018) Congenital toxoplasmosis: priorities for further health promotion action. Public Health 122(4):335\u2013353","journal-title":"Public Health"},{"issue":"1","key":"7308_CR10","first-page":"00115","volume":"34","author":"HM Elsheikha","year":"2021","unstructured":"Elsheikha HM, Marra CM, Zhu XQ (2021) Epidemiology, pathophysiology, diagnosis, and management of cerebral toxoplasmosis. Clin Microbiol Rev 34(1):00115\u201300119","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"7308_CR11","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1111\/j.1365-2958.2004.04407.x","volume":"55","author":"BJ Foth","year":"2005","unstructured":"Foth BJ, Stimmler LM, Handman E, Crabb BS, Hodder AN, McFadden GI (2005) The malaria parasite Plasmodium falciparum has only one pyruvate dehydrogenase complex, which is located in the apicoplast. Mol Microbiol 55(1):39\u201353","journal-title":"Mol Microbiol"},{"key":"7308_CR12","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/B978-0-444-53490-3.00008-X","volume":"114","author":"SK Halonen","year":"2013","unstructured":"Halonen SK, Weiss LM (2013) Toxoplasmosis. Handb Clin Neurol 114:125\u2013145","journal-title":"Handb Clin Neurol"},{"issue":"26","key":"7308_CR13","doi-asserted-by":"publisher","first-page":"11009","DOI":"10.1074\/jbc.M116.768176","volume":"292","author":"X Hu","year":"2017","unstructured":"Hu X, Binns D, Reese ML (2017) The coccidian parasites Toxoplasma and Neospora dysregulate mammalian lipid droplet biogenesis. J Biol Chem 292(26):11009\u201311020","journal-title":"J Biol Chem"},{"issue":"1","key":"7308_CR14","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/0005-2760(88)90253-6","volume":"958","author":"K Ichihara","year":"1988","unstructured":"Ichihara K, Takahashi T, Fujii S (1988) Diacylglycerol acyltransferase in maturing safflower seeds: its influences on the fatty acid composition of triacylglycerol and on the rate of triacylglycerol synthesis. Biochim Biophys Acta 958(1):125\u2013129","journal-title":"Biochim Biophys Acta"},{"key":"7308_CR15","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1186\/1471-2091-12-44","volume":"12","author":"F Jing","year":"2011","unstructured":"Jing F, Cantu DC, Tvaruzkova J, Chipman JP, Nikolau BJ, Yandeau-Nelson MD, Reilly PJ (2011) Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity. BMC Biochem 12:44","journal-title":"BMC Biochem"},{"issue":"2","key":"7308_CR16","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1016\/j.chom.2020.01.002","volume":"27","author":"A Krishnan","year":"2020","unstructured":"Krishnan A, Kloehn J, Lunghi M, Chiappino-Pepe A, Waldman BS, Nicolas D, Varesio E, Hehl A, Lourido S, Hatzimanikatis V, Soldati-Favre D (2020) Functional and computational genomics reveal unprecedented flexibility in stage-specific Toxoplasma metabolism. Cell Host Microbe 27(2):290\u2013306","journal-title":"Cell Host Microbe"},{"issue":"22","key":"7308_CR17","doi-asserted-by":"publisher","first-page":"7743","DOI":"10.1074\/jbc.RA120.013004","volume":"295","author":"X Liang","year":"2020","unstructured":"Liang X, Cui J, Yang X, Xia N, Li Y, Zhao J, Gupta N, Shen B (2020) Acquisition of exogenous fatty acids renders apicoplast-based biosynthesis dispensable in tachyzoites of Toxoplasma. J Biol Chem 295(22):7743\u20137752","journal-title":"J Biol Chem"},{"issue":"4","key":"7308_CR18","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1111\/mmi.12485","volume":"91","author":"SE Lindner","year":"2014","unstructured":"Lindner SE, Sartain MJ, Hayes K, Harupa A, Moritz RL, Kappe SH, Vaughan AM (2014) Enzymes involved in plastid-targeted phosphatidic acid synthesis are essential for Plasmodium yoelii liver-stage development. Mol Microbiol 91(4):679\u2013693","journal-title":"Mol Microbiol"},{"key":"7308_CR19","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1186\/s13071-019-3464-1","volume":"12","author":"M Liu","year":"2019","unstructured":"Liu M, Li FX, Li CY, Li XC, Chen LF, Wu K, Yang PL, Lai ZF, Liu TK, Sullivan WJ Jr, Cui L, Chen XG (2019) Characterization of protein arginine methyltransferase of TgPRMT5 in Toxoplasma gondii. Parasit Vectors 12:221","journal-title":"Parasit Vectors"},{"issue":"24","key":"7308_CR20","first-page":"3490","volume":"18","author":"JI MacRae","year":"2012","unstructured":"MacRae JI, Mar\u00e9chal E, Biot C, Bott\u00e9 CY (2012) The apicoplast: a key target to cure malaria. Curr Pharm Des 18(24):3490\u20133504","journal-title":"Curr Pharm Des"},{"key":"7308_CR21","doi-asserted-by":"publisher","first-page":"20163860","DOI":"10.1542\/peds.2016-3860","volume":"139","author":"YA Maldonado","year":"2017","unstructured":"Maldonado YA, Read JS (2017) Diagnosis, treatment, and prevention of congenital toxoplasmosis in the United States. Pediatrics 139:20163860","journal-title":"Pediatrics"},{"issue":"35","key":"7308_CR22","doi-asserted-by":"publisher","first-page":"13192","DOI":"10.1073\/pnas.0603391103","volume":"103","author":"J Mazumdar","year":"2006","unstructured":"Mazumdar J, Wilson EH, Masek K, Hunter CA, Striepen B (2006) Apicoplast fatty acid synthesis is essential for organelle biogenesis and parasite survival in Toxoplasma gondii. Proc Natl Acad Sci U S A 103(35):13192\u201313197","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"7308_CR23","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.1128\/EC.00255-07","volume":"6","author":"J Mazumdar","year":"2007","unstructured":"Mazumdar J, Striepen B (2007) Make it or take it: fatty acid metabolism of apicomplexan parasites. Eukaryot Cell 6(10):1727\u20131735","journal-title":"Eukaryot Cell"},{"issue":"6","key":"7308_CR24","doi-asserted-by":"publisher","first-page":"1006362","DOI":"10.1371\/journal.ppat.1006362","volume":"13","author":"SJ Nolan","year":"2017","unstructured":"Nolan SJ, Romano JD, Coppens I (2017) Host lipid droplets: an important source of lipids salvaged by the intracellular parasite Toxoplasma gondii. PLoS Pathog 13(6):1006362","journal-title":"PLoS Pathog"},{"key":"7308_CR25","first-page":"00347","volume":"24","author":"SJ Nolan","year":"2018","unstructured":"Nolan SJ, Romano JD, Kline JT, Coppens I (2018) Novel approaches to kill Toxoplasma by exploiting the uncontrolled uptake of unsaturated fatty acids and vulnerability to lipid storage inhibition of the parasite. Antimicrob Agents Chemother 24:00347\u201300418","journal-title":"Antimicrob Agents Chemother"},{"key":"7308_CR26","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1007\/s00726-010-0702-4","volume":"40","author":"Y Perez-Cervera","year":"2011","unstructured":"Perez-Cervera Y, Harichaux G, Schmidt J, Debierre-Grockiego F, Dehennaut V, Bieker U, Meurice E, Lefebvre T, Schwarz RT (2011) Direct evidence of O-GlcNAcylation in the apicomplexan Toxoplasma gondii: a biochemical and bioinformatic study. Amino Acids 40:847\u2013856","journal-title":"Amino Acids"},{"key":"7308_CR27","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.molbiopara.2004.08.004","volume":"138","author":"F Quittnat","year":"2004","unstructured":"Quittnat F, Nishikawa Y, Stedman TT, Voelker DR, Choi JY, Zahn MM, Murphy RC, Barkley RM, Pypaert M, Joiner KA, Coppens I (2004) On the biogenesis of lipid bodies in ancient eukaryotes: synthesis of triacylglycerols by a Toxoplasma DGAT1-related enzyme. Mol Biochem Parasitol 138:107\u2013122","journal-title":"Mol Biochem Parasitol"},{"issue":"3","key":"7308_CR28","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1038\/nrmicro843","volume":"2","author":"SA Ralph","year":"2004","unstructured":"Ralph SA, van Dooren GG, Waller RF, Crawford MJ, Fraunholz MJ, Foth BJ, Tonkin CJ, Roos DS, McFadden GI (2004) Tropical infectious diseases: metabolic maps and functions of the Plasmodium falciparum apicoplast. Nat Rev Microbiol 2(3):203\u2013216","journal-title":"Nat Rev Microbiol"},{"key":"7308_CR29","doi-asserted-by":"publisher","first-page":"4957","DOI":"10.1074\/jbc.M111.310144","volume":"287","author":"S Ramakrishnan","year":"2011","unstructured":"Ramakrishnan S, Docampo MD, Macrae JI, Pujol FM, Brooks CF, van Dooren GG, Hiltunen JK, Kastaniotis AJ, McConville MJ, Striepen B (2011) Apicoplast and endoplasmic reticulum cooperate in fatty acid biosynthesis in apicomplexan parasite Toxoplasma gondii. J Biol Chem 287:4957\u20134971","journal-title":"J Biol Chem"},{"issue":"4","key":"7308_CR30","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1016\/j.plipres.2013.06.003","volume":"52","author":"S Ramakrishnan","year":"2013","unstructured":"Ramakrishnan S, Serricchio M, Striepen B, B\u00fctikofer P (2013) Lipid synthesis in protozoan parasites: a comparison between kinetoplastids and apicomplexans. Prog Lipid Res 52(4):488\u2013512","journal-title":"Prog Lipid Res"},{"issue":"1\u20132","key":"7308_CR31","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.molbiopara.2015.03.004","volume":"199","author":"MJ Shears","year":"2015","unstructured":"Shears MJ, Bott\u00e9 CY, McFadden GI (2015) Fatty acid metabolism in the Plasmodium apicoplast: drugs, doubts and knockouts. Mol Biochem Parasitol 199(1\u20132):34\u201350","journal-title":"Mol Biochem Parasitol"},{"key":"7308_CR32","first-page":"01114-14","volume":"5","author":"B Shen","year":"2014","unstructured":"Shen B, Brown KM, Lee TD, Sibley LD (2014) Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR\/CAS9. mBio 5:01114\u201314","journal-title":"mBio"},{"issue":"6390","key":"7308_CR33","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1038\/359082a0","volume":"359","author":"LD Sibley","year":"1992","unstructured":"Sibley LD, Boothroyd JC (1992) Virulent strains of Toxoplasma gondii comprise a single clonal lineage. Nature 359(6390):82\u201385","journal-title":"Nature"},{"key":"7308_CR34","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.ijpara.2020.11.001","volume":"51","author":"NC Smith","year":"2021","unstructured":"Smith NC, Goulart C, Hayward JA, Kupz A, Miller CM, van Dooren GG (2021) Control of human toxoplasmosis. Int J Parasitol 51:95\u2013121","journal-title":"Int J Parasitol"},{"key":"7308_CR35","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1042\/bse0510111","volume":"51","author":"B Striepen","year":"2011","unstructured":"Striepen B (2011) The apicoplast: a red alga in human parasites. Essays Biochem 51:111\u2013125","journal-title":"Essays Biochem"},{"issue":"3","key":"7308_CR36","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1111\/j.1462-5822.2008.01270.x","volume":"11","author":"AM Vaughan","year":"2009","unstructured":"Vaughan AM, O\u2019Neill MT, Tarun AS, Camargo N, Phuong TM, Aly AS, Cowman AF, Kappe SH (2009) Type II fatty acid synthesis is essential only for malaria parasite late liver stage development. Cell Microbiol 11(3):506\u2013520","journal-title":"Cell Microbiol"},{"key":"7308_CR37","doi-asserted-by":"publisher","first-page":"3165","DOI":"10.1096\/fj.201901416RR","volume":"34","author":"JL Wang","year":"2020","unstructured":"Wang JL, Bai MJ, Elsheikha HM, Liang QL, Li TT, Cao XZ, Zhu XQ (2020) Novel roles of dense granule protein 12 (GRA12) in Toxoplasma gondii infection. FASEB J 34:3165\u20133178","journal-title":"FASEB J"},{"key":"7308_CR38","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1007\/s00436-015-4791-6","volume":"115","author":"JL Wang","year":"2016","unstructured":"Wang JL, Huang SY, Li TT, Chen K, Ning HR, Zhu XQ (2016) Evaluation of the basic functions of six calcium-dependent protein kinases in Toxoplasma gondii using CRISPR-Cas9 system. Parasitol Res 115:697\u2013702","journal-title":"Parasitol Res"},{"key":"7308_CR39","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1093\/infdis\/jiy211","volume":"218","author":"JL Wang","year":"2018","unstructured":"Wang JL, Li TT, Elsheikha HM, Chen K, Cong W, Yang WB, Bai MJ, Huang SY, Zhu XQ (2018) Live attenuated Pru:1cdpk2 strain of Toxoplasma gondii protects against acute, chronic, and congenital toxoplasmosis. J Infect Dis 218:768\u2013777","journal-title":"J Infect Dis"},{"key":"7308_CR40","first-page":"84","volume":"8","author":"JL Wang","year":"2017","unstructured":"Wang JL, Li TT, Elsheikha HM, Chen K, Zhu WN, Yue DM, Zhu XQ, Huang SY (2017) Functional characterization of rhoptry kinome in the virulent Toxoplasma gondii RH strain. Front Microbiol 8:84","journal-title":"Front Microbiol"},{"key":"7308_CR41","doi-asserted-by":"crossref","unstructured":"Xu XP, Elsheikha HM, Liu WG, Zhang ZW, Sun LX, Liang QL, Song MX, Zhu XQ (2021) The role of type II fatty acid synthesis enzymes FabZ, ODSCI, and ODSCII in the pathogenesis of Toxoplasma gondii infection. Front Microbiol 12:703059","DOI":"10.3389\/fmicb.2021.703059"},{"key":"7308_CR42","doi-asserted-by":"publisher","first-page":"2210","DOI":"10.3389\/fmicb.2017.02210","volume":"8","author":"J Yang","year":"2017","unstructured":"Yang J, Zhang L, Diao H, Xia N, Zhou Y, Zhao J, Shen B (2017) ANK1 and DnaK-TPR, two tetratricopeptide repeat-containing proteins primarily expressed in Toxoplasma bradyzoites, do not contribute to bradyzoite differentiation. Front Microbiol 8:2210","journal-title":"Front Microbiol"},{"key":"7308_CR43","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1016\/j.bbalip.2012.08.018","volume":"1831","author":"J Yao","year":"2013","unstructured":"Yao J, Rock CO (2013) Phosphatidic acid synthesis in bacteria. Biochim Biophys Acta 1831:495\u2013502","journal-title":"Biochim Biophys Acta"},{"issue":"11","key":"7308_CR44","doi-asserted-by":"publisher","first-page":"2283","DOI":"10.1194\/jlr.R800018-JLR200","volume":"49","author":"CL Yen","year":"2008","unstructured":"Yen CL, Stone SJ, Koliwad S, Harris C, Farese RV Jr (2008) Thematic review series: glycerolipids. DGAT enzymes and triacylglycerol biosynthesis. J Lipid Res 49(11):2283\u20132301","journal-title":"J Lipid Res"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-021-07308-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00436-021-07308-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-021-07308-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T11:27:46Z","timestamp":1634210866000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00436-021-07308-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,9]]},"references-count":44,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["7308"],"URL":"https:\/\/doi.org\/10.1007\/s00436-021-07308-3","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,9]]},"assertion":[{"value":"25 June 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 September 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The present study was reviewed and approved by the Animal Ethics Committee of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. All animals were handled strictly according to the Animal Ethics Procedures and Guidelines of the People\u2019s Republic of China.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}