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Historically, rumen fluke infections in Europe were assumed to be caused mainly by <jats:italic>Paramphistomum cervi<\/jats:italic> (or species, which were later considered to be synonymous with <jats:italic>P. cervi<\/jats:italic>), but more recently molecular studies demonstrated <jats:italic>Calicophoron daubneyi<\/jats:italic> to be the predominating species. For the present investigation, adult rumen flukes isolated from 23 cattle originating from ten farms in Germany (Saxony [1], Baden-W\u00fcrttemberg [4], Bavaria [5]) and one farm in Austria (Tyrol) were analyzed to establish partial sequences of the mitochondrial cytochrome c oxidase subunit I (<jats:italic>COI<\/jats:italic>) and the complete sequence of the nuclear internal transcribed spacer 2 (<jats:italic>ITS2<\/jats:italic>). Flukes of five animals (dairy cows from three farms in Bavaria) were determined as <jats:italic>P. leydeni<\/jats:italic>, and flukes of 18 animals (dairy cows or cattle from cow-calf operations from eight farms in Saxony [1], Baden-W\u00fcrttemberg [4], Bavaria [2], and Tyrol [1]) were identified as <jats:italic>C. daubneyi<\/jats:italic>. Based on the molecular analysis of adult rumen flukes collected from cattle, the results of this investigation confirm the common occurrence of <jats:italic>C. daubneyi<\/jats:italic> in Germany and reveal the first definitive findings of <jats:italic>P. leydeni<\/jats:italic> in Germany and <jats:italic>C. daubneyi<\/jats:italic> in Austria.<\/jats:p>","DOI":"10.1007\/s00436-021-07344-z","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T14:14:10Z","timestamp":1635171250000},"page":"4061-4066","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["DNA barcoding of rumen flukes (Paramphistomidae) from bovines in Germany and Austria"],"prefix":"10.1007","volume":"120","author":[{"given":"Sandra","family":"Wiedermann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Harl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Fuehrer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Mayr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7757-1002","authenticated-orcid":false,"given":"Barbara","family":"Hinney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Rehbein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,18]]},"reference":[{"key":"7344_CR1","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1515\/helmin-2015-0011","volume":"52","author":"G Duscher","year":"2015","unstructured":"Duscher G, Harl J, Fuehrer H (2015) Evidence of Troglotrema acutum and Skrjabingylus sp. coinfection in a polecat from Lower Austria. 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