{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:30:05Z","timestamp":1762432205434,"version":"3.37.3"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T00:00:00Z","timestamp":1645142400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T00:00:00Z","timestamp":1645142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"universidad de antioquia","award":["5-1-890"],"award-info":[{"award-number":["5-1-890"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2022,5]]},"DOI":"10.1007\/s00436-022-07467-x","type":"journal-article","created":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T05:02:42Z","timestamp":1645160562000},"page":"1419-1428","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Utility of the combination of hederagenin glucoside saponins and chromane hydrazone in the topical treatment of canine cutaneous leishmaniasis. An observational study"],"prefix":"10.1007","volume":"121","author":[{"given":"S. P.","family":"Piragauta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. L.","family":"Higuita-Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Arbel\u00e1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Restrepo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Archbold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Qui\u00f1ones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Echeverri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Escobar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. D.","family":"V\u00e9lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Montoya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2752-4931","authenticated-orcid":false,"given":"S. M.","family":"Robledo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,2,18]]},"reference":[{"issue":"19","key":"7467_CR1","first-page":"28","volume":"84","author":"CM Aguilar","year":"1990","unstructured":"Aguilar CM, Rangel EF, Garcia L et al (1990) Zoonotic cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis associated with domestic animals in Venezuela and Brazil. Mem Inst Oswaldo Cruz 84(19):28","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"261","key":"7467_CR2","first-page":"263","volume":"87","author":"AA Ahuja","year":"2012","unstructured":"Ahuja AA, Bumb RA, Mehta RD et al (2012) Successful treatment of canine cutaneous leishmaniasis using radio-frequency induced heat (RFH) therapy. Am J Trop Med Hyg 87(261):263","journal-title":"Am J Trop Med Hyg"},{"issue":"175","key":"7467_CR3","first-page":"180","volume":"5","author":"EGO Barbosa Santos","year":"1998","unstructured":"Barbosa Santos EGO, Marzochi MCA, Concei\u00e7\u00e3o NF et al (1998) N-methylglucamine antimonate (Sbv+): intralesional canine tegumentary leishmaniasis therapy. Parasite 5(175):180","journal-title":"Parasite"},{"key":"7467_CR4","doi-asserted-by":"publisher","first-page":"1878","DOI":"10.1056\/NEJM200006223422506","volume":"342","author":"K Benson","year":"1886","unstructured":"Benson K (1886) Hartz AJ 2000 A comparison of observational studies and randomized, controlled trials. New Engl J Med 342:1878","journal-title":"New Engl J Med"},{"issue":"108","key":"7467_CR5","first-page":"120","volume":"162","author":"A Blavier","year":"2001","unstructured":"Blavier A, Keroack S, Denerolle P et al (2001) Atypical forms of canine leishmaniosis. Vet J 162(108):120","journal-title":"Vet J"},{"issue":"1","key":"7467_CR6","first-page":"4","volume":"25","author":"F Dantas-Torres","year":"2019","unstructured":"Dantas-Torres F, Mir\u00f3 G, Baneth G et al (2019) Canine leishmaniasis control in the context of one health. Emerg Infect Dis 25(1):4","journal-title":"Emerg Infect Dis"},{"key":"7467_CR7","unstructured":"Echeverri F, Quinones W, Torres F, et al 2015 Saponins and chromans derivatives mixture compositions against leishmaniasis, trypanosomiasis americana, malaria, trypanosomiasis africana and Fasciola hepatica. USPTO 9168268B1"},{"issue":"90","key":"7467_CR8","first-page":"105","volume":"7","author":"K Ita","year":"2015","unstructured":"Ita K (2015) Transdermal delivery of drugs with microneedles, potential and challenges. Pharmaceutics 7(90):105","journal-title":"Pharmaceutics"},{"issue":"441","key":"7467_CR9","first-page":"445","volume":"182","author":"L Manna","year":"2009","unstructured":"Manna L, Vitale F, Reale S et al (2009) Study of efficacy of miltefosine and allopurinol in dogs with leishmaniasis. Vet J 182(441):445","journal-title":"Vet J"},{"key":"7467_CR10","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Valencia AJ, Daza-Rivera CF, Rosales-Chilama M, et al 2017 Clinical and parasitological factors in parasite persistence after treatment and clinical cure of cutaneous leishmaniasis. PLoS Negl Trop Dis 11 e0005713","DOI":"10.1371\/journal.pntd.0005713"},{"issue":"1018","key":"7467_CR11","first-page":"23","volume":"189","author":"MG Mendon\u00e7a","year":"2004","unstructured":"Mendon\u00e7a MG, de Brito ME, Rodrigues EH et al (2004) Persistence of leishmania parasites in scars after clinical cure of American cutaneous leishmaniasis: is there a sterile cure? J Infect Dis 189(1018):23","journal-title":"J Infect Dis"},{"key":"7467_CR12","doi-asserted-by":"crossref","unstructured":"Montalvo AM, Fraga J, Montano I, et al 2016 Molecular identification of Leishmania spp. clinical isolates from Colombia based on hsp70 gene. Biomedica 36 37 44","DOI":"10.7705\/biomedica.v36i2.2688"},{"key":"7467_CR13","doi-asserted-by":"crossref","unstructured":"Montalvo AM, Fraga J, Tirado D, et al 2017 Detection and identification of Leishmania spp.: application of two hsp70-based PCR-RFLP protocols to clinical samples from the New World. Parasitol Res 116 1843 1848","DOI":"10.1007\/s00436-017-5454-6"},{"issue":"1281","key":"7467_CR14","first-page":"1290","volume":"17","author":"M Murgu","year":"2006","unstructured":"Murgu M, Rodrigues-Filho E (2006) Dereplication of Glycosides from Sapindus saponaria. J Braz Chem Soc 17(1281):1290","journal-title":"J Braz Chem Soc"},{"key":"7467_CR15","unstructured":"OECD 2004 Test No. 427: Skin Absorption: In Vivo Method. OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris"},{"key":"7467_CR16","unstructured":"OECD 2017 Test No. 402: Acute Dermal Toxicity, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris"},{"key":"7467_CR17","unstructured":"OECD. 2019a Test No. 431: In vitro skin corrosion: reconstructed human epidermis (RHE) test method, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris"},{"key":"7467_CR18","unstructured":"OECD (2019b) Test No. 439: In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris"},{"key":"7467_CR19","doi-asserted-by":"crossref","unstructured":"Patino LH, Mendez C, Rodriguez O, et al 2017 Spatial distribution,\u00a0Leishmania\u00a0species and clinical traits of cutaneous leishmaniasis cases in the Colombian army. PLoS Negl Trop Dis 11 e0005876","DOI":"10.1371\/journal.pntd.0005876"},{"key":"7467_CR20","doi-asserted-by":"crossref","unstructured":"Percie du Sert N, Hurst V, Ahluwalia A, et al (2020) The ARRIVE guidelines 2.0: updated guidelines for reporting animal research. BMC Vet Res 16:242","DOI":"10.1186\/s12917-020-02451-y"},{"issue":"132","key":"7467_CR21","first-page":"137","volume":"57","author":"LOR Pereira","year":"2017","unstructured":"Pereira LOR, Moreira RB, de Oliveira MP et al (2017) Is Leishmania (Viannia) braziliensis parasite load associated with disease pathogenesis? Int J Infect Dis 57(132):137","journal-title":"Int J Infect Dis"},{"key":"7467_CR22","doi-asserted-by":"publisher","first-page":"28266","DOI":"10.1038\/srep28266","volume":"6","author":"JJ Ram\u00edrez","year":"2016","unstructured":"Ram\u00edrez JJ, Hern\u00e1ndez C, Le\u00f3n CM et al (2016) Taxonomy, diversity, temporal and geographical distribution of cutaneous leishmaniasis in Colombia: a retrospective study. Sci Rep 6:28266","journal-title":"Sci Rep"},{"key":"7467_CR23","doi-asserted-by":"publisher","first-page":"148","DOI":"10.3390\/pharmaceutics10030148","volume":"10","author":"C Salamanca","year":"2018","unstructured":"Salamanca C, Barrera-Ocampo A, Lasso J et al (2018) Franz diffusion cell approach for pre-formulation characterization of ketoprofen semi-solid dosage forms. Pharmaceutics 10:148","journal-title":"Pharmaceutics"},{"key":"7467_CR24","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez Buitrago JA, Silva Herrera LJ 2008 Estudio silvicultural de la especie Sapindus saponaria L. (jaboncillo) como base para su aprovechamiento silvoindustrial. Colombia Forestal 11 71 81","DOI":"10.14483\/udistrital.jour.colomb.for.2008.1.a05"},{"issue":"57","key":"7467_CR25","first-page":"60","volume":"40","author":"F Sasani","year":"2016","unstructured":"Sasani F, Javanbakht J, Samani R et al (2016) Canine Cutaneous Leishmaniasis J Parasit Dis 40(57):60","journal-title":"Canine Cutaneous Leishmaniasis J Parasit Dis"},{"issue":"824","key":"7467_CR26","first-page":"827","volume":"58","author":"A Schubach","year":"1998","unstructured":"Schubach A, Marzochi MC, Cuzzi-Maya T et al (1998) Cutaneous scars in American tegumentary leishmaniasis patients: a site of Leishmania (Viannia) braziliensis persistence and viability eleven years after antimonial therapy and clinical cure. Am J Trop Med Hygiene 58(824):827","journal-title":"Am J Trop Med Hygiene"},{"issue":"7","key":"7467_CR27","first-page":"12","volume":"34","author":"BL Travi","year":"2014","unstructured":"Travi BL (2014) Ethical and epidemiological dilemmas in the treatment of dogs for visceral leishmaniasis in Latin America. Biomedica 34(7):12","journal-title":"Biomedica"},{"key":"7467_CR28","doi-asserted-by":"crossref","unstructured":"Upegui Y, Rios K, Qui\u00f1ones W, et al 2019 Chroman-4-one hydrazones derivatives: synthesis, characterization, and in vitro and in vivo antileishmanial effects. Med Chem Res 28 2184 2199","DOI":"10.1007\/s00044-019-02446-x"},{"key":"7467_CR29","doi-asserted-by":"crossref","unstructured":"Upegui Y, Echeverri F, Qui\u00f1ones W, et al 2020 Mode of action of a formulation containing hydrazones and saponins against Leishmania spp. Role in mitochondria, proteases, and reinfection process. Int J Parasitol Drugs Drug Resistance 13 94 106","DOI":"10.1016\/j.ijpddr.2020.06.004"},{"issue":"173","key":"7467_CR30","first-page":"181","volume":"12","author":"A V\u00e1squez-Trujillo","year":"2008","unstructured":"V\u00e1squez-Trujillo A, Gonz\u00e1lez AE, G\u00f3ngora A et al (2008) Leishmania (Viannia) guyanensis identification in canines from rural locality in the Villavicencio municipallity. Meta-Colombia Rev Orinoquia 12(173):181","journal-title":"Meta-Colombia Rev Orinoquia"},{"issue":"351","key":"7467_CR31","first-page":"356","volume":"83","author":"I V\u00e9lez","year":"2010","unstructured":"V\u00e9lez I, L\u00f3pez L, S\u00e1nchez X et al (2010) Efficacy of miltefosine for the treatment of American cutaneous leishmaniasis. Am J Trop Med Hyg 83(351):356","journal-title":"Am J Trop Med Hyg"},{"issue":"807","key":"7467_CR32","first-page":"811","volume":"86","author":"ID V\u00e9lez","year":"2012","unstructured":"V\u00e9lez ID, Carrillo LM, L\u00f3pez L et al (2012) An epidemic outbreak of canine cutaneous leishmaniasis in Colombia caused by Leishmania braziliensis and Leishmania panamensis. Am J Trop Med Hyg 86(807):811","journal-title":"Am J Trop Med Hyg"},{"key":"7467_CR33","unstructured":"World Health Organization 2010 Control of the leishmaniases World Health Organ Tech Rep Ser 949 1 186"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-022-07467-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00436-022-07467-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-022-07467-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T12:08:18Z","timestamp":1649419698000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00436-022-07467-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,18]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["7467"],"URL":"https:\/\/doi.org\/10.1007\/s00436-022-07467-x","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"type":"print","value":"0932-0113"},{"type":"electronic","value":"1432-1955"}],"subject":[],"published":{"date-parts":[[2022,2,18]]},"assertion":[{"value":"28 July 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 February 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 February 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}