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In order to monitor ticks and tick-borne pathogens, the \u201cTekenscanner\u201d (Dutch for Tick scanner), a citizen science project, was launched in The Netherlands. It is a smartphone application for pet-owners to get ticks from their dog or cat, identified and checked for pathogens for free. At the same time, information about the pet and the geographic location of tick infestation becomes available for research. The application was launched in 2018, and the results of the first 6\u00a0months after launch of the app were reported. Ticks were identified based on morphology, and DNA was extracted and amplified by a panel of tick-borne pathogen-specific primers. Next, the amplicons were subjected to reverse line blot with specific probes for important pathogens to determine their presence or absence. The present paper describes the results of 2019 and 2020. There were 2260 ticks collected from 871 dogs and 255 cats (26 ticks were from an unknown host) and all pet owners were informed about the results. Four species of ticks were collected: <jats:italic>Ixodes ricinus<\/jats:italic> (90.0%), <jats:italic>Ixodes hexagonus<\/jats:italic> (7.3%), <jats:italic>Dermacentor reticulatus<\/jats:italic> (2.8%) and <jats:italic>Rhipicephalus sanguineus<\/jats:italic> (0.1%). <jats:italic>Ixodes ricinus<\/jats:italic> was the tick with the most divergent pathogens: <jats:italic>Anaplasma<\/jats:italic> sp. (1.3%), <jats:italic>Babesia<\/jats:italic> sp. (0.8%), <jats:italic>Borrelia<\/jats:italic> spp. (4.8%), <jats:italic>Neoehrlichia<\/jats:italic> sp. (3.7%) and <jats:italic>Rickettsia helvetica<\/jats:italic> (12.6%). In <jats:italic>I. hexagonus, R. helvetica<\/jats:italic> (1.8%) and <jats:italic>Babesia<\/jats:italic> sp. (0.6%) were detected and <jats:italic>Rickettsia raoultii<\/jats:italic> in <jats:italic>D. reticulatus<\/jats:italic> (16.2%). One of the two nymphs of <jats:italic>R. sanguineus<\/jats:italic> was co-infected with <jats:italic>Borrelia<\/jats:italic> and <jats:italic>R. helvetica<\/jats:italic> and the other one was uninfected. The high numbers of different pathogens found in this study suggest that companion animals, by definition synanthropic animals, and their ticks can serve as sentinels for emerging tick-borne pathogens.<\/jats:p>","DOI":"10.1007\/s00436-022-07518-3","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T04:30:41Z","timestamp":1650601841000},"page":"1887-1893","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Monitoring of ticks and their pathogens from companion animals obtained by the \u201ctekenscanner\u201d application in The Netherlands"],"prefix":"10.1007","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3625-3432","authenticated-orcid":false,"given":"F. N. 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