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Infection results in significant economic costs to the Australian sheep industry due to reduced wool quality caused by chronic itching from sheep rubbing and biting fleece. Treatment relies on use of insecticides; however, resistance has developed against pyrethroid and other insect growth regulator lousicides. There is urgent need to develop cost-effective lice management to reduce the use of insecticides, with the application of insecticidal treatments only applied when an infestation is detected. However, the current detection method relies on fleece parting for detection of <jats:italic>B. ovis<\/jats:italic> which is highly dependent on the skill of the inspector, the number of sheep examined, and the prevalence and severity of the infestation. To improve <jats:italic>B. ovis<\/jats:italic> detection, a highly sensitive (5 \u00d7 10<jats:sup>\u22128<\/jats:sup> ng\/\u03bcL) and specific multiplex quantitative PCR which simultaneously detects sheep lice and sheep DNA was developed. In addition, a <jats:italic>B. ovis<\/jats:italic> loop-mediated isothermal amplification (LAMP) assay was developed for field use. The <jats:italic>B. ovis<\/jats:italic> LAMP (Bov-LAMP) assay was optimized to reliably detect <jats:italic>B. ovis<\/jats:italic> from wool samples down to 5 \u00d7 10<jats:sup>\u22126<\/jats:sup> ng\/\u03bcL, with time to positive (T<jats:sub>p<\/jats:sub>) &lt; 10 min. Both assays demonstrate high sensitivity and specificity, enabling rapid identification of <jats:italic>B. ovis<\/jats:italic> DNA from sheep fleece samples and have the capacity to be used for ongoing management and surveillance of <jats:italic>B. ovis<\/jats:italic> in Australian sheep flocks.<\/jats:p>","DOI":"10.1007\/s00436-022-07520-9","type":"journal-article","created":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T07:03:24Z","timestamp":1650265404000},"page":"1597-1606","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of molecular detection methods of Bovicola ovis from sheep fleece"],"prefix":"10.1007","volume":"121","author":[{"given":"Lily","family":"Tran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant T.","family":"Rawlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4550-2277","authenticated-orcid":false,"given":"Travis","family":"Beddoe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,4,18]]},"reference":[{"issue":"3","key":"7520_CR1","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.fsigen.2011.08.006","volume":"6","author":"R Alaeddini","year":"2012","unstructured":"Alaeddini R (2012) Forensic implications of PCR inhibition\u2014a review. 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