{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:44:52Z","timestamp":1793277892365,"version":"4.5.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"11","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001769","name":"Charles Sturt University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001769","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract\n<\/jats:title><jats:p>Platyhelminths belonging to the family Clinostomidae (Digenea) have a worldwide distribution and are known to infect piscivorous birds through their intermediate hosts, usually fish species. In the present study, clinostome metacercariae were collected from fish hosts, including <jats:italic>Channa punctata<\/jats:italic> (Bloch 1793) (<jats:italic>n<\/jats:italic>\u2009=\u200925) and <jats:italic>Trichogaster fasciata<\/jats:italic> Bloch and Schneider 1801 (<jats:italic>n<\/jats:italic>\u2009=\u200925), from a freshwater system in India. The experimental infection of cattle egrets, <jats:italic>Ardea<\/jats:italic> (<jats:italic>Bubulcus<\/jats:italic>) <jats:italic>ibis<\/jats:italic> Linnaeus 1758, with some of the live metacercariae found in the present study was successful. Live adult parasites were obtained from the buccal cavity of the birds. Both metacercaria and adult specimens were subjected to molecular studies to obtain the sequences of 28S, ITS1, and ITS2 (nuclear rDNA) regions. The parasites were found to belong to three species, <jats:italic>Clinostomum giganticum<\/jats:italic> Agarwal 1959; <jats:italic>C. piscidium<\/jats:italic> Southwell and Prashad 1918; and <jats:italic>Euclinostomum heterostomum<\/jats:italic> (Rudolphi 1809). Phylogenetic analyses of the sequences obtained from the adults and metacercariae established a link between the metacercariae in the fish hosts and adults in the avian host, which is essential to elucidate their partial life cycle and specify morphological characteristics in the metacercarial stage.<\/jats:p>","DOI":"10.1007\/s00436-022-07644-y","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T07:04:07Z","timestamp":1662534247000},"page":"3083-3089","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India"],"prefix":"10.1007","volume":"121","author":[{"given":"Kirti","family":"Choudhary","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailendra","family":"Ray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8606-6400","authenticated-orcid":false,"given":"Shokoofeh","family":"Shamsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirupama","family":"Agrawal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"issue":"1\/2","key":"7644_CR1","first-page":"75","volume":"11","author":"S Agarwal","year":"1960","unstructured":"Agarwal S (1960) Studies on the morphology, systematics and life history of Clinostomum giganticum n. sp. (Trematoda: Clinostomatidae). Indian J Helminthol 11(1\/2):75\u2013115","journal-title":"Indian J Helminthol"},{"key":"7644_CR2","first-page":"147","volume":"7","author":"F Aghlmandi","year":"2018","unstructured":"Aghlmandi F, Habibi F, Afraii MA, Abdoli A, Shamsi S (2018) Infection with metacercaria of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes from Southern Caspian Sea Basin. Rev M\u00e9d V\u00e9t 7:147\u2013151","journal-title":"Rev M\u00e9d V\u00e9t"},{"key":"7644_CR3","doi-asserted-by":"crossref","unstructured":"Bhalerao G (1942) Some metacercarial forms of Clinostomatidae (Trematoda) from India. Proc Indian Acad Sci-Section B Vol 16. Springer India, p 67\u201371","DOI":"10.1007\/BF03046951"},{"issue":"2","key":"7644_CR4","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1006\/mpev.1995.1011","volume":"4","author":"J Bowles","year":"1995","unstructured":"Bowles J, Blair D, McManus DP (1995) A molecular phylogeny of the human schistosomes. Mol Phylogenet Evol 4(2):103\u2013109. https:\/\/doi.org\/10.1006\/mpev.1995.1011","journal-title":"Mol Phylogenet Evol"},{"issue":"4","key":"7644_CR5","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1017\/S0022149X18000445","volume":"93","author":"R Briosio-Aguilar","year":"2019","unstructured":"Briosio-Aguilar R, Garc\u00eda-Varela M, Hern\u00e1ndez-Mena D, Rubio-Godoy M, De Le\u00f3n GP-P (2019) Morphological and molecular characterization of an enigmatic clinostomid trematode (Digenea: Clinostomidae) parasitic as metacercariae in the body cavity of freshwater fishes (Cichlidae) across Middle America. J Helminthol 93(4):461\u2013474","journal-title":"J Helminthol"},{"issue":"4","key":"7644_CR6","doi-asserted-by":"publisher","first-page":"575","DOI":"10.2307\/3284227","volume":"83","author":"AO Bush","year":"1997","unstructured":"Bush AO, Lafferty KD, Lotz JM, Shostak AW (1997) Parasitology meets ecology on its own terms: Margolis et al revisited. J Parasitol 83(4):575\u2013583. https:\/\/doi.org\/10.2307\/3284227","journal-title":"J Parasitol"},{"issue":"3","key":"7644_CR7","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1017\/S0022149X13000151","volume":"88","author":"M Caffara","year":"2014","unstructured":"Caffara M, Bruni G, Paoletti C, Gustinelli A, Fioravanti M (2014) Metacercariae of Clinostomum complanatum (Trematoda: Digenea) in European newts Triturus carnifex and Lissotriton vulgaris (Caudata: Salamandridae). J Helminthol 88(3):278\u2013285","journal-title":"J Helminthol"},{"key":"7644_CR8","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1051\/parasite\/2014034","volume":"21","author":"M Caffara","year":"2014","unstructured":"Caffara M, Davidovich N, Falk R, Smirnov M, Ofek T, Cummings D, Gustinelli A, Fioravanti ML (2014) Redescription of Clinostomum phalacrocoracis metacercariae (Digenea: Clinostomidae) in cichlids from Lake Kinneret Israel. Parasite 21:32. https:\/\/doi.org\/10.1051\/parasite\/2014034","journal-title":"Parasite"},{"issue":"2","key":"7644_CR9","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1645\/15-823","volume":"102","author":"M Caffara","year":"2016","unstructured":"Caffara M, Locke SA, Cristanini C, Davidovich N, Markovich MP, Fioravanti ML (2016) A combined morphometric and molecular approach to identifying metacercariae of Euclinostomum heterostomum (Digenea: Clinostomidae). J Parasitol 102(2):239\u2013248","journal-title":"J Parasitol"},{"issue":"3","key":"7644_CR10","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1007\/s00436-019-06586-2","volume":"119","author":"M Caffara","year":"2020","unstructured":"Caffara M, Locke SA, Echi PC, Halajian A, Luus-Powell WJ, Benini D, Tedesco P, Fioravanti ML (2020) A new species of Clinostomum Leidy, 1856 based on molecular and morphological analysis of metacercariae from African siluriform fishes. Parasitol Res 119(3):885\u2013892","journal-title":"Parasitol Res"},{"issue":"4","key":"7644_CR11","doi-asserted-by":"publisher","first-page":"238","DOI":"10.2478\/s11686-006-0037-1","volume":"51","author":"SS Curran","year":"2006","unstructured":"Curran SS, Tkach VV, Overstreet RM (2006) A review of Polylekithum Arnold, 1934 and its familial affinities using morphological and molecular data, with description of Polylekithum catahoulensis sp. nov. Acta Parasitol 51(4):238\u2013248. https:\/\/doi.org\/10.2478\/s11686-006-0037-1","journal-title":"Acta Parasitol"},{"key":"7644_CR12","unstructured":"Fernando CH, Furtado JI, Gussev AV, Hanek G, Kakonge SA (1972) Methods for the study of freshwater fish parasites. University of Waterloo series. No 12"},{"issue":"2","key":"7644_CR13","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1093\/sysbio\/42.2.182","volume":"42","author":"DM Hillis","year":"1993","unstructured":"Hillis DM, Bull JJ, Felsenstein J, Kishino H (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Is there something wrong with the bootstrap on phylogenies? A reply to Hillis and Bull. Syst Biol 42(2):182\u2013200","journal-title":"Syst Biol"},{"issue":"9","key":"7644_CR14","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1016\/S0020-7519(98)00117-9","volume":"28","author":"O Jousson","year":"1998","unstructured":"Jousson O, Bartoli P, Zaninetti L, Pawlowski J (1998) Use of the ITS rDNA for elucidation of some life-cycles of Mesometridae (Trematoda, Digenea). Int J Parasitol 28(9):1403\u20131411. https:\/\/doi.org\/10.1016\/S0020-7519(98)00117-9","journal-title":"Int J Parasitol"},{"key":"7644_CR15","unstructured":"Kagei N, Yanohara Y, Uchikawa R, Sato A (1988) Natural infection with Clinostomum complanatum Rud. 1819 in the birds of southern Japan. Jpn J Parasitol 37(4):254\u2013257"},{"key":"7644_CR16","doi-asserted-by":"crossref","unstructured":"Kanev I, Radev V, Fried B (2002) Family Clinostomidae L\u00fche, 1901. In:  Keys to the Trematoda, vol 1, Editors: Gibson DI, Jones A, Bray RA. CAB International and the Natural History Museum, Wallingford, UK:113\u2013120","DOI":"10.1079\/9780851995472.0113"},{"issue":"2","key":"7644_CR17","first-page":"37","volume":"6","author":"H Kamo","year":"1962","unstructured":"Kamo H, Ogino K, Hatsushika R (1962) A unique infection of man with Clinostomum sp., a small trematode causing acute laryngitis. Yonago Acta Med 6(2):37\u201340","journal-title":"Yonago Acta Med"},{"issue":"3\u20134","key":"7644_CR18","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.vetpar.2010.08.027","volume":"174","author":"WM Lotfy","year":"2010","unstructured":"Lotfy WM, Brant SV, Ashmawy KI, Devkota R, Mkoji GM, Loker ES (2010) A molecular approach for identification of paramphistomes from Africa and Asia. Vet Parasitol 174(3\u20134):234\u2013240. https:\/\/doi.org\/10.1016\/j.vetpar.2010.08.027","journal-title":"Vet Parasitol"},{"key":"7644_CR19","first-page":"93","volume":"21","author":"A Mitchell","year":"1995","unstructured":"Mitchell A (1995) Yellow grubs and other problems associated with aquatic birds. Aquac Mag 21:93\u201397","journal-title":"Aquac Mag"},{"issue":"5","key":"7644_CR20","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1016\/S0020-7519(00)00039-4","volume":"30","author":"I Mollaret","year":"2000","unstructured":"Mollaret I, Lim LHS, Justine J-L (2000) Phylogenetic position of the monogeneans Sundanonchus, Thaparocleidus, and Cichlidogyrus inferred from 28S rDNA sequences. Int J Parasitol 30(5):659\u2013662. https:\/\/doi.org\/10.1016\/S0020-7519(00)00039-4","journal-title":"Int J Parasitol"},{"key":"7644_CR21","doi-asserted-by":"publisher","unstructured":"Montes M, Plaul S, Croci Y, Waldbillig M, Ferrari W, Topa E, Martorelli S (2020) Pathology associated with three new Clinostomum metacercariae from Argentina with morphological and DNA barcode identification. J Helminthol 94(e148):1\u201311. https:\/\/doi.org\/10.1017\/S0022149X20000292","DOI":"10.1017\/S0022149X20000292"},{"key":"7644_CR22","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/S0065-308X(05)60002-4","volume":"60","author":"MJ Nolan","year":"2005","unstructured":"Nolan MJ, Cribb TH (2005) The use and implications of ribosomal DNA sequencing for the discrimination of digenean species. Adv Parasitol 60:101\u2013163","journal-title":"Adv Parasitol"},{"key":"7644_CR23","unstructured":"Pandey K, Baugh S (1969) Studies on clinostome metacercariae. II. A restudy of Clinostomum piscidium from metacercaria and adult. Zool Anz 183(5\/6):463\u2013480"},{"issue":"4","key":"7644_CR24","doi-asserted-by":"publisher","first-page":"401","DOI":"10.3347\/kjp.2009.47.4.401","volume":"47","author":"CW Park","year":"2009","unstructured":"Park CW, Kim JS, Joo HS, Kim J (2009) A human case of Clinostomum complanatum infection in Korea. Korean J Parasitol 47(4):401\u2013404. https:\/\/doi.org\/10.3347\/kjp.2009.47.4.401","journal-title":"Korean J Parasitol"},{"issue":"3","key":"7644_CR25","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/S1995-7645(14)60022-5","volume":"7","author":"A Phalee","year":"2014","unstructured":"Phalee A, Wongsawad C (2014) Prevalence of infection and molecular confirmation by using ITS-2 region of Fasciola gigantica found in domestic cattle from Chiang Mai province, Thailand. Asian Pac J Trop Med 7(3):207\u2013211. https:\/\/doi.org\/10.1016\/S1995-7645(14)60022-5","journal-title":"Asian Pac J Trop Med"},{"key":"7644_CR26","doi-asserted-by":"publisher","first-page":"3585","DOI":"10.1007\/s00436-020-06892-0","volume":"119","author":"AR Rahmati","year":"2020","unstructured":"Rahmati AR, Kiani B, Afshari A, Moghaddas E, Williams M, Shamsi S (2020) World-wide prevalence of Anisakis larvae in fish and its relationship to human allergic anisakiasis: a systematic review. Parasitol Res 119:3585\u20133594. https:\/\/doi.org\/10.1007\/s00436-020-06892-0","journal-title":"Parasitol Res"},{"key":"7644_CR27","doi-asserted-by":"crossref","unstructured":"Rosser TG, Baumgartner WA, Alberson NR, Noto TW, Woodyard ET, King DT, Wise DJ, Griffin MJ (2018) Clinostomum poteae n. sp. (Digenea: Clinostomidae), in the trachea of a double-crested cormorant Phalacrocorax auritus Lesson, 1831 and molecular data linking the life-cycle stages of Clinostomum album Rosser, Alberson, Woodyard, Cunningham, Pote & Griffin, 2017 in Mississippi, USA. Syst Parasitol 95(6):543\u2013566","DOI":"10.1007\/s11230-018-9801-5"},{"issue":"3","key":"7644_CR28","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1645\/13-285.1","volume":"100","author":"S Senapin","year":"2014","unstructured":"Senapin S, Phiwsaiya K, Laosinchai P, Kowasupat C, Ruenwongsa P, Panijpan B (2014) Phylogenetic analysis of parasitic trematodes of the genus Euclinostomum found in trichopsis and betta fish. J Parasitol 100(3):368\u2013371. https:\/\/doi.org\/10.1645\/13-285.1","journal-title":"J Parasitol"},{"key":"7644_CR29","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.mcp.2010.09.003","volume":"25","author":"S Shamsi","year":"2011","unstructured":"Shamsi S, Gasser RB, Beveridge I (2011) Mutation scanning-coupled sequencing of nuclear ribosomal DNA spacers (as a taxonomic tool) for the specific identification of different Contracaecum (Nematoda: Anisakidae) larval types. Mol Cell Probes 25:13\u201318","journal-title":"Mol Cell Probes"},{"issue":"2","key":"7644_CR30","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1016\/j.rvsc.2013.06.018","volume":"95","author":"S Shamsi","year":"2013","unstructured":"Shamsi S, Halajian A, Tavakol S, Mortazavi P, Boulton J (2013) Pathogenicity of Clinostomum complanatum (Digenea: Clinostomidae) in piscivorous birds. Res Vet Sci 95(2):537\u2013539. https:\/\/doi.org\/10.1016\/j.rvsc.2013.06.018","journal-title":"Res Vet Sci"},{"key":"7644_CR31","doi-asserted-by":"publisher","unstructured":"Shamsi S, Barton DP, Day S, Masiga J, Zhu X, McLellan M (2021a) Characterization of Clinostomum sp. (Trematoda: Clinostomidae) infecting cormorants in south-eastern Australia. Parasitol Res 120:2793\u20132803. https:\/\/doi.org\/10.1007\/s00436-021-07246-0","DOI":"10.1007\/s00436-021-07246-0"},{"key":"7644_CR32","doi-asserted-by":"publisher","unstructured":"Shamsi S, Day S, Zhu X, McLellan M, Barton DP, Dang M, Nowak BF (2021b) Wild fish as reservoirs of parasites on Australian Murray Cod farms. Aquaculture 539:736584.\u00a0https:\/\/doi.org\/10.1016\/j.aquaculture.2021.736584","DOI":"10.1016\/j.aquaculture.2021.736584"},{"issue":"1","key":"7644_CR33","first-page":"12","volume":"29","author":"R Singh","year":"1959","unstructured":"Singh R (1959) Studies on the morphology and life history of Clinostomum piscidium Southwell and Prashad, 1918 (Trematoda: Clinostomatidae). Proc Nat Acad Sciences India, Section B Biol Sci 29(1):12\u201333","journal-title":"Proc Nat Acad Sciences India, Section B Biol Sci"},{"key":"7644_CR34","doi-asserted-by":"crossref","unstructured":"Sutili FJ, Gressler LT, de Pelegrini LFV (2014) Clinostomum complanatum (Trematoda, Digenea): a parasite of birds and fishes with zoonotic potential in southern Brazil. A Review. Revista Brasileira de Higiene e Sanidade Animal: RBHSA 8(1):99\u2013114","DOI":"10.5935\/1981-2965.20140007"},{"key":"7644_CR35","doi-asserted-by":"publisher","unstructured":"Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725\u20132729 https:\/\/doi.org\/10.1093\/molbev\/mst197","DOI":"10.1093\/molbev\/mst197"},{"issue":"22","key":"7644_CR36","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","volume":"22","author":"JD Thompson","year":"1994","unstructured":"Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22(22):4673\u20134680. https:\/\/doi.org\/10.1093\/nar\/22.22.4673","journal-title":"Nucleic Acids Res"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-022-07644-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00436-022-07644-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-022-07644-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T18:05:44Z","timestamp":1665597944000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00436-022-07644-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,7]]},"references-count":36,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["7644"],"URL":"https:\/\/doi.org\/10.1007\/s00436-022-07644-y","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,7]]},"assertion":[{"value":"7 February 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 August 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 September 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The corresponding author (SS) is on the editorial board of the journal.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}