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Dogs are the main domestic reservoir of zoonotic visceral leishmaniasis, and the role of secondary vectors such as ticks and fleas and particularly Rhipicephalus sanguineus (the brown dog tick) in transmitting <jats:italic>L. infantum<\/jats:italic> has been investigated. In the present paper, the presence of <jats:italic>Leishmania<\/jats:italic> DNA was investigated in questing <jats:italic>Ixodes ricinus<\/jats:italic> ticks collected from 4 rural areas included in three parks of the Emilia-Romagna Region (north-eastern Italy), where active foci of human visceral leishmaniasis have been identified. The analyses were performed on 236 DNA extracts from 7 females, 6 males, 72 nymph pools, and 151 larvae pools. Four samples (1.7%) (i.e., one larva pool, 2 nymph pools, and one adult male) tested positive for <jats:italic>Leishmania<\/jats:italic> kDNA. To the best of our knowledge, this is the first report of the presence of <jats:italic>Leishmania<\/jats:italic> kDNA in questing <jats:italic>I. ricinus<\/jats:italic> ticks collected from a rural environment. This finding in unfed larvae, nymphs, and adult male ticks supports the hypothesis that <jats:italic>L. infantum<\/jats:italic> can have both transstadial and transovarial passage in <jats:italic>I. ricinus<\/jats:italic> ticks. The potential role of <jats:italic>I. ricinus<\/jats:italic> ticks in the sylvatic cycle of leishmaniasis should be further investigated.<\/jats:p>","DOI":"10.1007\/s00436-022-07655-9","type":"journal-article","created":{"date-parts":[[2022,9,9]],"date-time":"2022-09-09T02:02:52Z","timestamp":1662688972000},"page":"3331-3336","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Detection of Leishmania sp. kDNA in questing Ixodes ricinus (Acari, Ixodidae) from the Emilia-Romagna Region in northeastern Italy"],"prefix":"10.1007","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0154-9527","authenticated-orcid":false,"given":"Alice","family":"Magri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3429-6121","authenticated-orcid":false,"given":"Monica","family":"Caffara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8234-432X","authenticated-orcid":false,"given":"Marialetizia","family":"Fioravanti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6959-8731","authenticated-orcid":false,"given":"Roberta","family":"Galuppi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,9,9]]},"reference":[{"issue":"4","key":"7655_CR1","first-page":"329","volume":"38","author":"VA Almeida","year":"2016","unstructured":"Almeida VA, da Hora TN, J\u00fanior NFL, Carvalho FS, da Silva AL, Wenceslau AA, Albuquerque GR, Silva FL (2016) Detection of Leishmania infantum DNA in hamsters infested with ticks collected from naturally infected dogs. 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