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Moreover, there is limited information on coinfections between these parasites and on <jats:italic>L. infantum\u2019s<\/jats:italic> distribution in blood, skin and lymphoid tissue in cats. We used PCR and DNA sequencing to investigate the prevalence of these parasites and the aetiology of <jats:italic>Hepatozoon<\/jats:italic> spp. and <jats:italic>Babesia<\/jats:italic> spp., in blood, skin, spleen and lymph node samples from up to 212 stray cats and 82 abandoned dogs in southeast Spain. All except 2 dogs were healthy; instead, 112 cats had clinical signs. The estimated PCR prevalences (95% confidence interval) were 25% (19\u201331%) <jats:italic>Hepatozoon felis<\/jats:italic> in cats, 13% (6\u201321%) <jats:italic>Hepatozoon canis<\/jats:italic> in dogs, 1% (0\u20134%) <jats:italic>Babesia vogeli<\/jats:italic> in dogs, 0% <jats:italic>Babesia<\/jats:italic> spp. in cats and 21% (15\u201326%) and 44% (33\u201355%) <jats:italic>L. infantum<\/jats:italic> in cats and dogs, respectively, and infections were not associated with each other. <jats:italic>Leishmania infantum<\/jats:italic> prevalence in lymphoid tissue was significantly higher in dogs than in cats (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), and dogs had higher parasite loads than cats (<jats:italic>p<\/jats:italic>\u2009=\u20090.012). Moreover, <jats:italic>L. infantum<\/jats:italic> prevalence was significantly higher in the skin and lymphoid tissue compared to blood in infected, asymptomatic animals but it was similar in cats with clinical signs, which also had higher parasite loads compared to infected, asymptomatic cats (p\u2009&lt;\u20090.05). The study highlights significant differences between sympatric dogs and cats with respect to the parasite infections investigated, as well as the need to examine both lymphoid tissue and skin samples to maximise the sensitivity of <jats:italic>L. infantum<\/jats:italic> infection diagnosis.<\/jats:p>","DOI":"10.1007\/s00436-022-07705-2","type":"journal-article","created":{"date-parts":[[2022,11,5]],"date-time":"2022-11-05T06:02:31Z","timestamp":1667628151000},"page":"35-47","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Clinical, diagnostic and epidemiological implications of Hepatozoon spp., Babesia spp. and Leishmania infantum infection in cats and dogs in a Mediterranean periurban setting"],"prefix":"10.1007","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2994-1384","authenticated-orcid":false,"given":"Mar\u00eda","family":"Ortu\u00f1o","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Bernal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaarit","family":"Nachum-Biala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Risue\u00f1o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana","family":"Ortiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gad","family":"Baneth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Berriatua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,5]]},"reference":[{"issue":"4","key":"7705_CR1","doi-asserted-by":"publisher","first-page":"472","DOI":"10.3390\/pathogens10040472","volume":"10","author":"F Abramo","year":"2021","unstructured":"Abramo F, Albanese F, Gattuso S, Randone A, Fileccia I, Dedola C, Ibba F, Ottaiano P, Brianti E (2021) Skin lesions in feline leishmaniosis: a systematic review. 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