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The organism is genetically diverse, and so far, at least 28 subtypes (STs) have been identified with ST1\u2013ST9 being the most common in humans. The pathogenicity of\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    is controversial. Several routes of transmission have been proposed including fecal\u2013oral (e.g., zoonotic, anthroponotic) and waterborne. Research on the latter has gained traction in the last few years with the organism having been identified in various bodies of water, tap water, and rainwater collection containers including water that has been previously filtered and\/or chlorinated. Herein, we assessed the resistance of 11 strains maintained in culture, spanning ST1\u2013ST9 to various chlorine and hydrogen peroxide concentrations for 24\u00a0h, and performed recovery assays along with re-exposure. Following the treatment with both compounds, all subtypes showed increased resistance, and viability could be visualized at the cellular level. These results are hinting at the presence of mechanism of resistance to both chlorine and hydrogen peroxide. As such, this pilot study can be the platform for developing guidelines for water treatment processes.\n                  <\/jats:p>","DOI":"10.1007\/s00436-022-07713-2","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T04:02:41Z","timestamp":1668484961000},"page":"167-176","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Resistance of Blastocystis to chlorine and hydrogen peroxide"],"prefix":"10.1007","volume":"122","author":[{"given":"Rub\u00e9n","family":"Mart\u00edn-Escolano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geok Choo","family":"Ng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin S. W.","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Rune","family":"Stensvold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni","family":"Gentekaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasios D.","family":"Tsaousis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,15]]},"reference":[{"key":"7713_CR1","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1007\/s00436-002-0821-2","volume":"90","author":"N Abe","year":"2003","unstructured":"Abe N, Wu Z, Yoshikawa H (2003) Zoonotic genotypes of Blastocystis hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene. 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