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For this purpose, it is essential to generate data regarding the pathogen load of the meat to be consumed and handled. It is known that raccoons, particularly in Germany, show a high seroprevalence of<jats:italic>Toxoplasma gondii<\/jats:italic>. Because serological data only indicates contact of a host to a parasite additional direct detection is needed to prove presence of parasitic stages in particular tissues. Therefore, a total of 150 samples from raccoons with known serostatus were tested and quantified using magnetic-capture real-time PCR for<jats:italic>Toxoplasma gondii.<\/jats:italic>As it represents potentially consumption-relevant parts of raccoons, meat from forelimb and hindlimb was examined. Samples were stratified into three groups based on the animals\u2019 serostatus (each 50 negative, low positive, and high positive). All samples from seronegative animals were found negative by MC-PCR as well. In a total of 56 meat samples from 100 seropositive animals,<jats:italic>T. gondii<\/jats:italic>DNA was detected. Statistically significant more samples were positive by MC-PCR in the high positive than in the low positive serostatus group (38\/50 vs. 18\/50,<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Furthermore, samples from the former group were also found to have statistically significant higher DNA equivalent values compared to samples from the low positive serostatus group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). These results suggest that meat from seropositive raccoons may contain considerable numbers of<jats:italic>T. gondii<\/jats:italic>presenting a potential public health risk for humans whilst handling and consumption.<\/jats:p>","DOI":"10.1007\/s00436-022-07730-1","type":"journal-article","created":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T09:03:59Z","timestamp":1668848639000},"page":"307-313","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Direct detection and quantification of Toxoplasma gondii in meat samples from feral raccoons (Procyon lotor) in Germany by magnetic-capture real-time PCR"],"prefix":"10.1007","volume":"122","author":[{"given":"Lydia","family":"Engel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Hamedy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Koethe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,19]]},"reference":[{"key":"7730_CR1","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1016\/j.ijpara.2017.05.005","volume":"47","author":"IG Algaba","year":"2017","unstructured":"Algaba IG, Geerts M, Jennes M, Coucke W, Opsteegh M, Cox E, Dorny P, Dierick K, de Craeye S (2017) A more sensitive, efficient and ISO 17025 validated magnetic capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat. 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