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For the research, blood samples of 330 dogs tested positive for dirofilariosis using concentration tests or \u201crapid heartworm tests\u201d were sent by private veterinary practitioners for further DNA analyses and species determination. The results revealed an unquestionable change in the pattern of <jats:italic>Dirofilaria<\/jats:italic> species distribution with <jats:italic>Dirofilaria immitis<\/jats:italic>, diagnosed as the agent of mono- and co-infections with <jats:italic>D. repens<\/jats:italic>, responsible for 60.00% of all cases. The results showed that in the course of the last 5 years, <jats:italic>D. immitis<\/jats:italic> has spread significantly in Slovakia and has become the dominant causal agent of dirofilariosis in the former endemic areas of <jats:italic>D. repens<\/jats:italic> distribution, which increases infection risk for both dogs and humans.<\/jats:p>","DOI":"10.1007\/s00436-023-07983-4","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T13:01:46Z","timestamp":1695992506000},"page":"2945-2950","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dirofilaria immitis conquering the regions in Slovakia previously endemic for D. repens"],"prefix":"10.1007","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3742-948X","authenticated-orcid":false,"given":"Martina","family":"Miterp\u00e1kov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6381-3282","authenticated-orcid":false,"given":"Daniela","family":"Valentov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6816-2992","authenticated-orcid":false,"given":"Zuzana","family":"Hurn\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,9,29]]},"reference":[{"key":"7983_CR1","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1556\/004.2022.00006","volume":"70","author":"Z Becker","year":"2022","unstructured":"Becker Z, Holl\u00f3 N, Farkas R, Gyurkovszky M, Reiczigel J, Olaszy K, V\u00e1ri Z, V\u00f6r\u00f6s K (2022) Serodiagnostic difficulties and possibilities of heartworm disease in regions where both Dirofilaria immitis and Dirofilaria repens infections occur. 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